大豆及其加工食品转基因成分检测技术研究进展
|
摘要
随着基因工程技术在食用农产品领域的应用发展,形形色色的转基因食品应运而生。目前包括我国在内的许多国家为保护消费者的知情权和选择权,都建立了转基因食品标识管理制度。科学有效的转基因食品检测技术是实现我国转基因产品标识管理的前提。我国食品成分多样且基质复杂,针对初级农产品的转基因成分检测技术并不完全适用于工业加工食品尤其是深加工食品。这对食品监管及技术检测部门提出了新的要求。本文根据技术原理的不同分类介绍了目前几种主要的食品中转基因成分检测技术,除介绍现在已经成熟应用的技术方法外,本文还重点介绍了国内学者新近研发出的几种新技术,为研究人员进一步研发新技术提供重要参考。
With the genetic engineering technology maturing into the field of edible agricultural products, a variety of genetically modified foods have emerged. At present, many countries, including China, have established a genetically modified food labeling management system to protect consumers' right to know and to choose. Scientific and effective genetically modified food testing technology is the premise for realizing the management of genetically modified products in China. Due to the diverse composition and complex matrix of genetically modified foods, the detection technology of genetically modified ingredients for primary agricultural products is not fully applicable to industrial processed foods, especially deep processed foods. This puts new requirements on food regulatory and technical testing departments. This paper introduced the current detection technologies of genetically modified components in several major foods according to the different classifications of technical principles. In addition to introducing the technical methods that had been matured and applied, this paper also introduced several new technologies newly developed by domestic scholars, in order to provide important reference for researchers to further develop new technologies.
With the genetic engineering technology maturing into the field of edible agricultural products, a variety of genetically modified foods have emerged. At present, many countries, including China, have established a genetically modified food labeling management system to protect consumers' right to know and to choose. Scientific and effective genetically modified food testing technology is the premise for realizing the management of genetically modified products in China. Due to the diverse composition and complex matrix of genetically modified foods, the detection technology of genetically modified ingredients for primary agricultural products is not fully applicable to industrial processed foods, especially deep processed foods. This puts new requirements on food regulatory and technical testing departments. This paper introduced the current detection technologies of genetically modified components in several major foods according to the different classifications of technical principles. In addition to introducing the technical methods that had been matured and applied, this paper also introduced several new technologies newly developed by domestic scholars, in order to provide important reference for researchers to further develop new technologies.
引文
[1]邵彪,周小兰,王琳琳,等.浅析肉制品中植物源性转基因成分风险[J].食品安全质量检测学报,2018,9(20):5275-5280.Shao B, Zhou XL, Wang LL, et al. Analysis of the risk of plant-derived transgenic components in meat products[J]. J Food Saf Qual, 2018,9(20):5275-5280.
[2]崔宁波,张正岩.转基因大豆研究及应用进展[J].西北农业学报,2016,25(8):1111-1124.Cui NB, Zhang ZY. Research and application progress of transgenic soybeans[J]. Northwest Agric J, 2016,25(8):1111-1124.
[3]王颢潜,陈锐,李夏莹,等.转基因产品成分检测技术研究进展[J].生物技术通报,2018, 34(3):31-38.Wang YQ, Chen R, Li XY, et al. Research progress in detection technology of genetically modified products[J]. Biotechnol Bull, 2018,34(3):31-38.
[4]杜智欣,焦悦,张亮亮,等.转基因成分定量检测技术研究进展[J].食品工业科技,2017, 38(10):379-384.Du ZX, Jiao Y, Zhang LL, et al. Research progress in quantitative detection technology of genetically modified components[J]. Sci Technol Food Ind, 2017, 38(10):379-384.
[5]韦红,朱暹明.转基因食品检测技术的应用与发展[J].食品安全导刊,2018,(26):72.Wei H, Zhu SM. Application and development of genetically modified food detection technology[J]. Chin Food Saf Magaz, 2018,(26):72.
[6]高新梅,逯敏慧.研究植物转基因技术的必要性探讨[J].现代农业科技.2019,(4):35-37Gao XM, Min MH. Discussion on the necessity of studying plant transgenic technology[J]. Mod Agric Sci Technol, 2019,(4):35-37.
[7]SN/T 3767. 3-2014出口食品中转基因成分环介导等温扩增(LAMP)检测方法第3部分:玉米Bt-11品系[S].SN/T 3767. 3-2014 Method for the detection of transgenic components in the export foods by loop-mediated isothermal amplification(LAMP)Part3:Maize Bt-11 strain[S].
[8]陈笑芸,汪小福,周育,等.转基因大豆深加工食品DNA鉴定技术研究[J].中国食品学报,2013, 13(4):156-162.Chen XY, Wang XF, Zhou Y, et al. Study on DNA identification technology of transgenic soybean deep processing food[J]. J Chin Instit Food Sci Technol, 2013,13(4):156-162.
[9]陈颖,王媛,葛毅强.GM食品内外源基因在加工过程中的降解变化[J].食品与发酵工业,2005,(8):66-69.Chen Y, Wang Y, Ge YQ. Degradation of exogenous genes in gm food during processing[J]. Food Ferment Ind, 2005,(8):66-69.
[10]王媛.转基因大豆内、外源基因在食品加工过程中变化规律的研究[D].北京:中国农业大学,2005.Wang Y. Study on the changes of internal and exogenous genes in transgenic soybeans during food processing[D]. Beijing:China Agricultural University, 2005.
[11]姚芹,徐泽鹏,曹灿灿,等.食用大豆油加工过程DNA降解及检测研究[J].食品科技,2018,43(4):184-188.Yao Q, Xu ZP, Cao CC, et al. DNA degradation and detection of edible soybean oil during processing[J]. Food Sci Technol, 2018, 43(4):184-188.
[12]刘艳艳,董书光,耿加亮,等.阿胶加工工艺过程中DNA降解规律研究[J].药学研究,2016, 35(9):501-507.Liu YY, Dong SG, Geng JL, et al. Study on DNA degradation in the process of Ejiao processing[J]. Pharm Res, 2016,35(9):501-507.
[13]叶可萍,周光宏,徐幸莲,等.转基因大豆不同加工过程DNA降解研究[J].食品科学,2010,31(13):312-315.Ye KP, Zhou GH, Xu XL, et al. DNA degradation of different processing processes of transgenic soybeans[J]. Food Sci, 2010,31(13):312-315.
[14]柴成梁,唐柏飞,常晓娇,等.利用微滴式数字PCR技术检测大豆毛油中的转基因成分[J/OL].中国粮油学报:1-5[2019-02-28]. http://kns.cnki. net/kcms/detail/11. 2864. TS. 20181228. 1659.002. html.Chai CL, Tang BF, Chang XJ, et al. Detection of transgenic components in soybean oil by microdroplet digital PCR[J/OL]. Journal of the Chinese Cereals and Oils Association:1-5[2019-02-28]. http://kns. cnki.net/kcms/detail/11. 2864. TS. 20181228. 1659.002. html.
[15] Genetically Modified Foods. Findings on genetically modified foods reported by investigators at Zhejiang academy of agricultural sciences(event-specific qualitative and quantitative detection of genetically modified rice G6H1)[Z]. Food Weekly News, 2019.
[16] Jun S, Bei N, Dong W, et al. Comparison of the Monte Carlo and guide to uncertainty in measurement methods in estimating measurement uncertainty:Indirect measurement of the CaMV35S promoter in mixed samples of genetically modified soybean[J]. Food Control, 2018,(2018):90.
[17]蔡翠霞,肖维威,康文杰,等.转基因食品DNA提取研究进展[J].中国生物工程杂志,2011,31(5):121-125.Cai CX, Xiao WW, Kang WJ, et al. Research progress in DNA extraction of genetically modified foods[J]. Chin J Biotechnol, 2011, 31(5):121-125.
[18]蔡翠霞,肖维威,吴慧慧,等.植物油DNA的高效提取方法研究[J].中国油脂,2014, 39(1):69-72Cai CX, Xiao WW, Wu HH, et al. Study on high efficient extraction method of vegetable oil DNA[J]. China Oils Fats, 2014,39(1):69-72.
[19]蔡翠霞,肖维威,康文杰,等.改良Chelex-100法快速提取转基因农产品DNA[J].生物技术通报,2011,(10):210-214.Cai CX, Xiao WW, Kang WJ, et al. Rapid extraction of DNA from transgenic agricultural products by modified Chelex-100 method[J].Biotechnol Bull, 2011,(10):210-214.
[20]刘欣,祝长青,王毅谦,等.大豆转基因检测中DNA提取方法的比较研究[J].食品科学,2013, 34(4):199-203.Liu X, Zhu CQ, Wang YQ, et al. Methodology comparison of DNA extraction from soybean for detection of genetically modified organism[J].Food Sci, 2013,34(4):199-203.
[21]王爱迪,冉晓华,陈磊,等.超强磁性微球提取深加工转基因食品DNA[J].食品科学,2013, 34(10):122-125.Wang AD, Yan XH, Chen L, et al. Extraction of deep processing genetically modified food dna by super magnetic microspheres[J]. Food Sci,2013,34(10):122-125.
[22]付海滨,闫超杰,李姝,等.数字PCR技术在转基因成分检测中的应用[J].辽宁农业科学,2017,(1):50-53.Fu HB, Yan CJ, Li W, et al. Application of digital pcr technology in detection of genetically modified components[J]. Liaoning Agric Sci,2017,(1):50-53.
[23]周杰,黄文胜,邓婷婷,等.转基因检测微流控恒温扩增芯片的研制[J].现代食品科技,2017, 33(6):293-302, 270.Zhou J, Huang WS, Deng TT, et al. Development of microfluidic constant temperature amplification chip for transgenic detection[J]. Mod Food Technol, 2017,33(6):293-302,270.
[24]魏霜,周广彪,刘津,等.多重串联式PCR基因碟片技术检测转基因大豆GTS 40-3-2[J].中国食品学报,2018,18(2):244-249Wei S, Zhou GB, Liu J, et al. Detection of transgenic soybean GTS 40-3-2by multiple tandem PCR gene disc technology[J]. Chin J Food Sci, 2018,18(2):244-249
[25] Genetics-DNA Research. New DNA Research Findings from S. Okumura and Co-Authors Described(Development of an Ultra-sensitive Detection Method for Genetically Modified Soybeans in Natto, a Traditional Japanese Fermented Food)[Z]. 2019.
[26] Zhou C, Sun CJ, Luo ZW, et al. Fiber optic biosensor for detection of genetically modified food based on catalytic hairpin assembly reaction and nanocomposites assisted signal amplification[J]. Sen Actuat B:Chem,2017,7:174.
[27] Cottenet G, Blancpain C, Sonnard, et al. Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food[J]. Food Chem, 2019, 274:760-765.
[28]敖金霞,高学军,于艳波,等.转基因大豆、玉米、水稻深加工产品的五重巢式PCR技术检测[J].中国农业大学学报,2010,15(2):93-99.Ao JX, Gao XJ, Yu YB, et al. Detection of five-fold nested PCR technology for transgenic soybean, com and rice deep processing products[J]. J China Agric Univ, 2010, 15(2):93-99.
[29]邓婷婷,黄文胜,葛毅强,等.基于微滴式和芯片式数字PCR技术的转基因克螟稻成分的定量检测[J/OL].食品科学:1-12[2018-08-13].http://kns. cnki. net/kcms/detail/11. 2206. TS. 20180713. 1700.006. html.Deng TT, Huang WS, Ge YQ, et al. Quantitative detection of transgenic glutinous rice components based on microdroplet and chip digital PCR technology[J/OL]. Food Science:1-12[2018-08-13]. http://kns. cnki.net/kcms/detail/11.2206. TS. 20180713. 1700. 006. html.
[30] Morisset D, Stebih D, Milavec M, et al. Quantitative analysis of food and feed samples with droplet digital PCR[J]. PLoS One, 2013, 8(5):e62583.
[31]李或媛,邢达,章春笋.连续流动微流控PCR快速检测转基因植物的实验研究[J].生物物理学报,2009,25(S1):498.Li YY, Xing D, Zhang CS. Experimental study on rapid detection of transgenic plants by continuous flow microfluidic PCR[J]. Acta Biophys Sin, 2009,25(S1):498.
[32] GB/T 33807-2017玉米中转基因成分的测定基因芯片法[S].GB/T 33807-2017 Determination of genetically modified components in maize-Gene chip method[S].
[33]蔡翠霞.转基因食品DNA提取及寡核苷酸芯片检测技术的研究[D].广州:南方医科大学,2012.Cai CX. DNA extraction and oligonucleotide chip detection technology of genetically modified foods[D]. Guangzhou:Southern Medical University,2012.
[34] GB 19495. 9-2017转基因产品检测植物产品液相芯片检测方法[S].GB 19495. 9-2017 Genetically modified product testing-Plant product liquid chip detection method[S].
[35]赵卫东,郑文杰,王爱迪,等.磁性微球在植物源性转基因食品检测中的应用[J].食品研究与开发,2012,33(6):128-130.Zhao WD, Zheng WJ, Wang AD, et al. Application of magnetic microspheres in the detection of plant-derived transgenic foods[J]. Food Res Dev, 2012,33(6):128-130.
[36]冉晓华.功能化聚合物微球的制备及其在转基因检测中的应用[D].天津:天津大学,2012.Ran XH. Preparation of functionalized polymer microspheres and its application in transgenic detection[D]. Tianjin:Tianjin University, 2012.
[37]环球网财经.阿胶检测不出驴的DNA成分是怎么回事?[EB/OL].[2016-03-18]. http://finance. huanqiu. com/roll/2016-03/8725111. html Global Network Finance. What is the DNA component of Ejiao detection?[EB/OL].[2016-03-18]. http://finance. huanqiu. com/roll/2016-03/8725111.html
[38]肖海龙,赵凯,林赛君,等.牛奶β-酪蛋白和大豆β-伴球蛋白双抗制备及夹心ELISA快速定性检测技术的建立[J].浙江大学学报(农业与生命科学版),2013, 39(2):222-226.Xiao HL, Zhao K, Lin SJ, et al. Preparation of milkβ-casein and soybeanβ-conglycinin double antibody and establishment of sandwich ELISA rapid qualitative detection technology[J]. J Zhejiang Univ(Agric Life Sci Ed), 2013,39(2):222-226.
[39] GB/T 19495. 8-2004转基因产品检测[S].GB/T 19495. 8-2004 Detection of genetically modified products[S].
[40]农业部1782号公告-12-2010转基因生物及其产品食用安全性检测蛋白质氨基酸序列飞行时间质谱分析方法[Z].Agriculture department No. 1782 announcement-12-2010 genetically modified organisms and their products edible safety testing protein amino acid sequence time-of-flight mass spectrometry[Z].
[41]汪琳,邢佑尚,周琦,等.3种转基因成分检测蛋白芯片的研制[J].植物检疫,2011,25(3):1-6.Wang L, Xing YS, Zhou Q, et al. Development of three kinds of transgenic component detection protein chips[J]. Plant Quar, 2011, 25(3):1-6.
[42]周晓光,任鲁风,李运涛,等.下一代测序技术:基因回顾与展望[J].中国科学:生命科学,2010,40(1):23-37.Zhou XG, Ren LF, Li YT, et al. Next generation sequencing technology:gene review and prospect[J]. Chin Sci:Life Sci, 2010,40(1):23-37.
[43] Akritidis P, Pasensis K, Tsaftaris A, et al. Identification of unknow genetically identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method[J]. Electr J Biotechnol, 2008, 11:76-83.
[44] Geoffrey C, Carine B, Veronique S, et al. Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms[J]. Anal BioanalChem, 2013,405(21):6831-6844.
[45] Yang L, Shen H, Pan A, et al. Screening and construct specific detection methods of transgenic Huafan No 1 tomato by conventional and real time PCR[J]. J Sci Food Agric,2005, 85(13):2159-2166.
[46] Nam HK, Ji YH, Hyang GL, et al. Strategic approaches to communicating with food consumers about genetically modified food[J]. Food Control,2018, 92:521-532.
[47]孟静,孙潇慧,钟立霞,等.豆制品中转基因成分检测的研究进展[J].大豆科学,2019, 38(1):148-152.Meng J, Sun WH, Zhong LX, et al. Research progress in detection of genetically modified organisms in bean products[J]. Soybean Sci, 2019,38(1):148-152.
[48]王邵宇.转基因大豆的发展及其风险探究[J].粮食科技与经济,2018,43(7):117-119.Wang SY. The development and risk of genetically modified soybeans[J].Food Sci Technol, 2018,43(7):117-119.
[2]崔宁波,张正岩.转基因大豆研究及应用进展[J].西北农业学报,2016,25(8):1111-1124.Cui NB, Zhang ZY. Research and application progress of transgenic soybeans[J]. Northwest Agric J, 2016,25(8):1111-1124.
[3]王颢潜,陈锐,李夏莹,等.转基因产品成分检测技术研究进展[J].生物技术通报,2018, 34(3):31-38.Wang YQ, Chen R, Li XY, et al. Research progress in detection technology of genetically modified products[J]. Biotechnol Bull, 2018,34(3):31-38.
[4]杜智欣,焦悦,张亮亮,等.转基因成分定量检测技术研究进展[J].食品工业科技,2017, 38(10):379-384.Du ZX, Jiao Y, Zhang LL, et al. Research progress in quantitative detection technology of genetically modified components[J]. Sci Technol Food Ind, 2017, 38(10):379-384.
[5]韦红,朱暹明.转基因食品检测技术的应用与发展[J].食品安全导刊,2018,(26):72.Wei H, Zhu SM. Application and development of genetically modified food detection technology[J]. Chin Food Saf Magaz, 2018,(26):72.
[6]高新梅,逯敏慧.研究植物转基因技术的必要性探讨[J].现代农业科技.2019,(4):35-37Gao XM, Min MH. Discussion on the necessity of studying plant transgenic technology[J]. Mod Agric Sci Technol, 2019,(4):35-37.
[7]SN/T 3767. 3-2014出口食品中转基因成分环介导等温扩增(LAMP)检测方法第3部分:玉米Bt-11品系[S].SN/T 3767. 3-2014 Method for the detection of transgenic components in the export foods by loop-mediated isothermal amplification(LAMP)Part3:Maize Bt-11 strain[S].
[8]陈笑芸,汪小福,周育,等.转基因大豆深加工食品DNA鉴定技术研究[J].中国食品学报,2013, 13(4):156-162.Chen XY, Wang XF, Zhou Y, et al. Study on DNA identification technology of transgenic soybean deep processing food[J]. J Chin Instit Food Sci Technol, 2013,13(4):156-162.
[9]陈颖,王媛,葛毅强.GM食品内外源基因在加工过程中的降解变化[J].食品与发酵工业,2005,(8):66-69.Chen Y, Wang Y, Ge YQ. Degradation of exogenous genes in gm food during processing[J]. Food Ferment Ind, 2005,(8):66-69.
[10]王媛.转基因大豆内、外源基因在食品加工过程中变化规律的研究[D].北京:中国农业大学,2005.Wang Y. Study on the changes of internal and exogenous genes in transgenic soybeans during food processing[D]. Beijing:China Agricultural University, 2005.
[11]姚芹,徐泽鹏,曹灿灿,等.食用大豆油加工过程DNA降解及检测研究[J].食品科技,2018,43(4):184-188.Yao Q, Xu ZP, Cao CC, et al. DNA degradation and detection of edible soybean oil during processing[J]. Food Sci Technol, 2018, 43(4):184-188.
[12]刘艳艳,董书光,耿加亮,等.阿胶加工工艺过程中DNA降解规律研究[J].药学研究,2016, 35(9):501-507.Liu YY, Dong SG, Geng JL, et al. Study on DNA degradation in the process of Ejiao processing[J]. Pharm Res, 2016,35(9):501-507.
[13]叶可萍,周光宏,徐幸莲,等.转基因大豆不同加工过程DNA降解研究[J].食品科学,2010,31(13):312-315.Ye KP, Zhou GH, Xu XL, et al. DNA degradation of different processing processes of transgenic soybeans[J]. Food Sci, 2010,31(13):312-315.
[14]柴成梁,唐柏飞,常晓娇,等.利用微滴式数字PCR技术检测大豆毛油中的转基因成分[J/OL].中国粮油学报:1-5[2019-02-28]. http://kns.cnki. net/kcms/detail/11. 2864. TS. 20181228. 1659.002. html.Chai CL, Tang BF, Chang XJ, et al. Detection of transgenic components in soybean oil by microdroplet digital PCR[J/OL]. Journal of the Chinese Cereals and Oils Association:1-5[2019-02-28]. http://kns. cnki.net/kcms/detail/11. 2864. TS. 20181228. 1659.002. html.
[15] Genetically Modified Foods. Findings on genetically modified foods reported by investigators at Zhejiang academy of agricultural sciences(event-specific qualitative and quantitative detection of genetically modified rice G6H1)[Z]. Food Weekly News, 2019.
[16] Jun S, Bei N, Dong W, et al. Comparison of the Monte Carlo and guide to uncertainty in measurement methods in estimating measurement uncertainty:Indirect measurement of the CaMV35S promoter in mixed samples of genetically modified soybean[J]. Food Control, 2018,(2018):90.
[17]蔡翠霞,肖维威,康文杰,等.转基因食品DNA提取研究进展[J].中国生物工程杂志,2011,31(5):121-125.Cai CX, Xiao WW, Kang WJ, et al. Research progress in DNA extraction of genetically modified foods[J]. Chin J Biotechnol, 2011, 31(5):121-125.
[18]蔡翠霞,肖维威,吴慧慧,等.植物油DNA的高效提取方法研究[J].中国油脂,2014, 39(1):69-72Cai CX, Xiao WW, Wu HH, et al. Study on high efficient extraction method of vegetable oil DNA[J]. China Oils Fats, 2014,39(1):69-72.
[19]蔡翠霞,肖维威,康文杰,等.改良Chelex-100法快速提取转基因农产品DNA[J].生物技术通报,2011,(10):210-214.Cai CX, Xiao WW, Kang WJ, et al. Rapid extraction of DNA from transgenic agricultural products by modified Chelex-100 method[J].Biotechnol Bull, 2011,(10):210-214.
[20]刘欣,祝长青,王毅谦,等.大豆转基因检测中DNA提取方法的比较研究[J].食品科学,2013, 34(4):199-203.Liu X, Zhu CQ, Wang YQ, et al. Methodology comparison of DNA extraction from soybean for detection of genetically modified organism[J].Food Sci, 2013,34(4):199-203.
[21]王爱迪,冉晓华,陈磊,等.超强磁性微球提取深加工转基因食品DNA[J].食品科学,2013, 34(10):122-125.Wang AD, Yan XH, Chen L, et al. Extraction of deep processing genetically modified food dna by super magnetic microspheres[J]. Food Sci,2013,34(10):122-125.
[22]付海滨,闫超杰,李姝,等.数字PCR技术在转基因成分检测中的应用[J].辽宁农业科学,2017,(1):50-53.Fu HB, Yan CJ, Li W, et al. Application of digital pcr technology in detection of genetically modified components[J]. Liaoning Agric Sci,2017,(1):50-53.
[23]周杰,黄文胜,邓婷婷,等.转基因检测微流控恒温扩增芯片的研制[J].现代食品科技,2017, 33(6):293-302, 270.Zhou J, Huang WS, Deng TT, et al. Development of microfluidic constant temperature amplification chip for transgenic detection[J]. Mod Food Technol, 2017,33(6):293-302,270.
[24]魏霜,周广彪,刘津,等.多重串联式PCR基因碟片技术检测转基因大豆GTS 40-3-2[J].中国食品学报,2018,18(2):244-249Wei S, Zhou GB, Liu J, et al. Detection of transgenic soybean GTS 40-3-2by multiple tandem PCR gene disc technology[J]. Chin J Food Sci, 2018,18(2):244-249
[25] Genetics-DNA Research. New DNA Research Findings from S. Okumura and Co-Authors Described(Development of an Ultra-sensitive Detection Method for Genetically Modified Soybeans in Natto, a Traditional Japanese Fermented Food)[Z]. 2019.
[26] Zhou C, Sun CJ, Luo ZW, et al. Fiber optic biosensor for detection of genetically modified food based on catalytic hairpin assembly reaction and nanocomposites assisted signal amplification[J]. Sen Actuat B:Chem,2017,7:174.
[27] Cottenet G, Blancpain C, Sonnard, et al. Two FAST multiplex real-time PCR reactions to assess the presence of genetically modified organisms in food[J]. Food Chem, 2019, 274:760-765.
[28]敖金霞,高学军,于艳波,等.转基因大豆、玉米、水稻深加工产品的五重巢式PCR技术检测[J].中国农业大学学报,2010,15(2):93-99.Ao JX, Gao XJ, Yu YB, et al. Detection of five-fold nested PCR technology for transgenic soybean, com and rice deep processing products[J]. J China Agric Univ, 2010, 15(2):93-99.
[29]邓婷婷,黄文胜,葛毅强,等.基于微滴式和芯片式数字PCR技术的转基因克螟稻成分的定量检测[J/OL].食品科学:1-12[2018-08-13].http://kns. cnki. net/kcms/detail/11. 2206. TS. 20180713. 1700.006. html.Deng TT, Huang WS, Ge YQ, et al. Quantitative detection of transgenic glutinous rice components based on microdroplet and chip digital PCR technology[J/OL]. Food Science:1-12[2018-08-13]. http://kns. cnki.net/kcms/detail/11.2206. TS. 20180713. 1700. 006. html.
[30] Morisset D, Stebih D, Milavec M, et al. Quantitative analysis of food and feed samples with droplet digital PCR[J]. PLoS One, 2013, 8(5):e62583.
[31]李或媛,邢达,章春笋.连续流动微流控PCR快速检测转基因植物的实验研究[J].生物物理学报,2009,25(S1):498.Li YY, Xing D, Zhang CS. Experimental study on rapid detection of transgenic plants by continuous flow microfluidic PCR[J]. Acta Biophys Sin, 2009,25(S1):498.
[32] GB/T 33807-2017玉米中转基因成分的测定基因芯片法[S].GB/T 33807-2017 Determination of genetically modified components in maize-Gene chip method[S].
[33]蔡翠霞.转基因食品DNA提取及寡核苷酸芯片检测技术的研究[D].广州:南方医科大学,2012.Cai CX. DNA extraction and oligonucleotide chip detection technology of genetically modified foods[D]. Guangzhou:Southern Medical University,2012.
[34] GB 19495. 9-2017转基因产品检测植物产品液相芯片检测方法[S].GB 19495. 9-2017 Genetically modified product testing-Plant product liquid chip detection method[S].
[35]赵卫东,郑文杰,王爱迪,等.磁性微球在植物源性转基因食品检测中的应用[J].食品研究与开发,2012,33(6):128-130.Zhao WD, Zheng WJ, Wang AD, et al. Application of magnetic microspheres in the detection of plant-derived transgenic foods[J]. Food Res Dev, 2012,33(6):128-130.
[36]冉晓华.功能化聚合物微球的制备及其在转基因检测中的应用[D].天津:天津大学,2012.Ran XH. Preparation of functionalized polymer microspheres and its application in transgenic detection[D]. Tianjin:Tianjin University, 2012.
[37]环球网财经.阿胶检测不出驴的DNA成分是怎么回事?[EB/OL].[2016-03-18]. http://finance. huanqiu. com/roll/2016-03/8725111. html Global Network Finance. What is the DNA component of Ejiao detection?[EB/OL].[2016-03-18]. http://finance. huanqiu. com/roll/2016-03/8725111.html
[38]肖海龙,赵凯,林赛君,等.牛奶β-酪蛋白和大豆β-伴球蛋白双抗制备及夹心ELISA快速定性检测技术的建立[J].浙江大学学报(农业与生命科学版),2013, 39(2):222-226.Xiao HL, Zhao K, Lin SJ, et al. Preparation of milkβ-casein and soybeanβ-conglycinin double antibody and establishment of sandwich ELISA rapid qualitative detection technology[J]. J Zhejiang Univ(Agric Life Sci Ed), 2013,39(2):222-226.
[39] GB/T 19495. 8-2004转基因产品检测[S].GB/T 19495. 8-2004 Detection of genetically modified products[S].
[40]农业部1782号公告-12-2010转基因生物及其产品食用安全性检测蛋白质氨基酸序列飞行时间质谱分析方法[Z].Agriculture department No. 1782 announcement-12-2010 genetically modified organisms and their products edible safety testing protein amino acid sequence time-of-flight mass spectrometry[Z].
[41]汪琳,邢佑尚,周琦,等.3种转基因成分检测蛋白芯片的研制[J].植物检疫,2011,25(3):1-6.Wang L, Xing YS, Zhou Q, et al. Development of three kinds of transgenic component detection protein chips[J]. Plant Quar, 2011, 25(3):1-6.
[42]周晓光,任鲁风,李运涛,等.下一代测序技术:基因回顾与展望[J].中国科学:生命科学,2010,40(1):23-37.Zhou XG, Ren LF, Li YT, et al. Next generation sequencing technology:gene review and prospect[J]. Chin Sci:Life Sci, 2010,40(1):23-37.
[43] Akritidis P, Pasensis K, Tsaftaris A, et al. Identification of unknow genetically identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method[J]. Electr J Biotechnol, 2008, 11:76-83.
[44] Geoffrey C, Carine B, Veronique S, et al. Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms[J]. Anal BioanalChem, 2013,405(21):6831-6844.
[45] Yang L, Shen H, Pan A, et al. Screening and construct specific detection methods of transgenic Huafan No 1 tomato by conventional and real time PCR[J]. J Sci Food Agric,2005, 85(13):2159-2166.
[46] Nam HK, Ji YH, Hyang GL, et al. Strategic approaches to communicating with food consumers about genetically modified food[J]. Food Control,2018, 92:521-532.
[47]孟静,孙潇慧,钟立霞,等.豆制品中转基因成分检测的研究进展[J].大豆科学,2019, 38(1):148-152.Meng J, Sun WH, Zhong LX, et al. Research progress in detection of genetically modified organisms in bean products[J]. Soybean Sci, 2019,38(1):148-152.
[48]王邵宇.转基因大豆的发展及其风险探究[J].粮食科技与经济,2018,43(7):117-119.Wang SY. The development and risk of genetically modified soybeans[J].Food Sci Technol, 2018,43(7):117-119.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |