油菜不同根肿病抗性材料的室内鉴定及相关生理分析
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摘要
结合田间种植的根肿病抗性表现,以菌土法和在蘸根法对多个疑似具有抗性的材料进行室内鉴定,初步筛选出两种对根肿菌4号生理小种具有抗性的油菜材料Kc84-1和OG-13;用病情统计的方法将Kc84-1与目前已推广种植的抗性材料华双5R和易感材料R-197进行对照验证,并对三种材料进行抗性生理分析.结果表明:Kc84-1与华双5R具有相同水平的根肿病抗性;不同材料的防御酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性以及抗病相关物质丙二醛(MDA)含量的表现各不相同,两种抗性材料在较高感病等级下的防御酶SOD、POD、PAL活性显著高于感病材料,PPO活性和MDA含量在抗性材料和易感材料之间没有显著差异.通过水培法观测三种材料感病早期的根毛侵染水平,结果表明,易感材料R-197的根毛侵染率在侵染第6 d到第14 d显著高于抗性材料华双5R和Kc84-1.
According to the resistance performance of clubroot in the field, two kinds of rapeseed materials, Kc84-1 and OG-13, which were resistant to the No.4 physiological race of Plasmodiophora brassicae, were preliminarily screened out by the bacterial soil method and the dipping root method among the several suspected disease resistant materials. The clubroot resistance of material Kc84-1 was identified by comparing with the resistant cultivar Huashuang 5 R and the susceptible material R-197 by the disease investigation statistics, and the related resistance physiological analysis of three materials was carried out. The results showed that, there was the same level clubroot resistance of Kc84-1 and Huashuang 5 R. The activity of defense enzymes Superoxide Dismutase(SOD), Peroxidase(POD), Polyphenol oxidase(PPO), Phenylalanine ammonialyase(PAL) and the Malondialdehyde(MDA) content of resistance related substances were different in distinct materials. The activities of the two kinds of resistant materials, such as SOD, POD, and PAL, were significantly higher than that of the susceptible material. There was no significant difference in PPO activity and MDA content between resistant materials and susceptible materials. The observation on root hair infection of three materials at an early stage by hydroponics, the results showed that the root hairs infection rate of susceptible material R-197 was significantly higher than that of resistant varieties Huashuang 5 R and material Kc84-1 in the sixth to fourteenth days of infection.
According to the resistance performance of clubroot in the field, two kinds of rapeseed materials, Kc84-1 and OG-13, which were resistant to the No.4 physiological race of Plasmodiophora brassicae, were preliminarily screened out by the bacterial soil method and the dipping root method among the several suspected disease resistant materials. The clubroot resistance of material Kc84-1 was identified by comparing with the resistant cultivar Huashuang 5 R and the susceptible material R-197 by the disease investigation statistics, and the related resistance physiological analysis of three materials was carried out. The results showed that, there was the same level clubroot resistance of Kc84-1 and Huashuang 5 R. The activity of defense enzymes Superoxide Dismutase(SOD), Peroxidase(POD), Polyphenol oxidase(PPO), Phenylalanine ammonialyase(PAL) and the Malondialdehyde(MDA) content of resistance related substances were different in distinct materials. The activities of the two kinds of resistant materials, such as SOD, POD, and PAL, were significantly higher than that of the susceptible material. There was no significant difference in PPO activity and MDA content between resistant materials and susceptible materials. The observation on root hair infection of three materials at an early stage by hydroponics, the results showed that the root hairs infection rate of susceptible material R-197 was significantly higher than that of resistant varieties Huashuang 5 R and material Kc84-1 in the sixth to fourteenth days of infection.
引文
[1] 李淼,檀根甲,承河元,等.植物抗病性研究现状与前景展望 [J].江西农业大学学报,2002,24:731.
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[3] Hwang S F,Strelkov S E,Feng J,et al.Plasmodiophora brassicae:a review of an emerging pathogen of the canadian canola (Brassica napus) crop [J].Mol Plant Pathol,2012,13:105.
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[6] Donald E C,Porter I J,Lancaster R A.Band incorporation of fluazinam (Shirlan) into soil to control clubroot of vegetable brassica crops [J].Aust J Expl Agr,2001,41:1223.
[7] 巩文峰,岳海梅,旺姆,等.林芝地区大白菜根肿病的药剂防治试验 [J].北方园艺,2012(12):165.
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[9] 刘勇,黄小琴,柯绍英,等.四川主栽油菜品种根肿病抗性研究 [J].中国油料作物学报,2009,31:90.
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[12] 肖崇刚.甘蓝根肿病菌的生物学特性研究 [J].菌物学报,2002,21:597.
[13] Dixon G R.Plasmodiophora brassicae in its environment [J].J Plant Growth Regul,2009,28:212.
[14] Tommerup I C,Ingram D S.The life-cycle of Plasmodiophora brassicae woron.in brassica tissue cultures and in intact roots [J].New Phytol,1971,70:327.
[15] 朱红丽,肖崇刚,陈国康,等.基于根肿菌早期侵染量的白菜抗性分析 [J].植物保护学报,2015,42:510.
[16] Rashid A,Ahmed H U,Xiao Q,et al.Effects of root exudates and pH on Plasmodiophora brassicae resting spore germination and infection of canola (Brassica napus L.) root hairs [J].Crop Prot,2013,48:16.
[17] Jutta L,Astrid S.What can we learn from clubroots:alterations in host roots and hormone homeostasis caused by Plasmodiophora brassicae [J].Eur J Plant Pathol,2008,121:291.
[18] Keen N T,Williams P H.Translocation of sugars into infected cabbage tissues during clubroot development [J].Plant Physiol,1969,44:748.
[19] Rodman J E.A taxonomic analysis of glucosinolate-producing plants,part 1:phenetics [J].Sys Bot,1991,16:598.
[20] Lovelock D A,Donald C E,Conlan X A,et al.Salicylic acid suppression of clubroot in broccoli (Brassicae oleracea;var.italica) caused by the obligate biotroph Plasmodiophora brassicae [J].Australas Plant Path,2013,42:141.
[21] David B,Astrid S,Jutta L,et al.Induction of trehalase in Arabidopsis plants infected with the trehalose-producing pathogen Plasmodiophora brassicae [J].Mol Plant Microbe Interact,2002,15:693.
[22] 况宁宁,张茹琴,鄢洪海.大白菜品种间根肿病抗性鉴定及抗性生理分析 [J].北方园艺,2012,11:140.
[23] 丁云花,简元才,余阳俊,等.我国8省市十字花科蔬菜根肿病菌生理小种的鉴定 [J].中国蔬菜,2013,1:85.
[24] 马丹丹,庞胜群,丁云花,等.甘蓝类蔬菜根肿病抗性鉴定 [J].石河子大学学报:自科版,2016,34:164.
[25] Asano T,Kageyama K,Hyakumachi M.Surface disinfestation of resting spores of Plasmodiophora brassicae used to infect hairy roots of Brassica spp.[J].Phytopathology,1999,89:314.引用本文格式:中文:蒋学飞,岳出,刘双双,等.油菜不同根肿病抗性材料的室内鉴定及相关生理分析 [J].四川大学学报:自然科学版,2019,56:573.英文:Jiang X F,Yue C,Liu S S,et al.Laboratory identification of different resistant materials with clubroot and related physiological analysis in rapeseed [J].J Sichuan Univ:Nat Sci Ed,2019,56:573.
[2] Kageyama K,Asano T,Dixon G R.Life cycle of Plasmodiophora brassicae [J].J Plant Growth Regul,2009,28:203.
[3] Hwang S F,Strelkov S E,Feng J,et al.Plasmodiophora brassicae:a review of an emerging pathogen of the canadian canola (Brassica napus) crop [J].Mol Plant Pathol,2012,13:105.
[4] Cavalier-Smith T,Chao E Y.Phylogeny and classification of phylum cercozoa (protozoa) [J].Protist,2003,154:341.
[5] 李金萍,柴阿丽,孙日飞,等.十字花科蔬菜根肿病研究新进展 [J].中国蔬菜,2012,1:1.
[6] Donald E C,Porter I J,Lancaster R A.Band incorporation of fluazinam (Shirlan) into soil to control clubroot of vegetable brassica crops [J].Aust J Expl Agr,2001,41:1223.
[7] 巩文峰,岳海梅,旺姆,等.林芝地区大白菜根肿病的药剂防治试验 [J].北方园艺,2012(12):165.
[8] 黄小琴,柯绍英,刘勇.四川省油菜区试材料对根肿病抗性差异研究 [J].西南农业学报,2008,21:349.
[9] 刘勇,黄小琴,柯绍英,等.四川主栽油菜品种根肿病抗性研究 [J].中国油料作物学报,2009,31:90.
[10] 张仕伟.十字花科蔬菜根肿病接种甘蓝型油菜研究初报 [J].云南农业科技,2006,6:42.
[11] Schwelm A,Fogelqvist J,Knaust A,et al.The Plasmodiophora brassicae genome reveals insights in its life cycle and ancestry of chitin synthases [J].Sci Rep,2015,5:11153.
[12] 肖崇刚.甘蓝根肿病菌的生物学特性研究 [J].菌物学报,2002,21:597.
[13] Dixon G R.Plasmodiophora brassicae in its environment [J].J Plant Growth Regul,2009,28:212.
[14] Tommerup I C,Ingram D S.The life-cycle of Plasmodiophora brassicae woron.in brassica tissue cultures and in intact roots [J].New Phytol,1971,70:327.
[15] 朱红丽,肖崇刚,陈国康,等.基于根肿菌早期侵染量的白菜抗性分析 [J].植物保护学报,2015,42:510.
[16] Rashid A,Ahmed H U,Xiao Q,et al.Effects of root exudates and pH on Plasmodiophora brassicae resting spore germination and infection of canola (Brassica napus L.) root hairs [J].Crop Prot,2013,48:16.
[17] Jutta L,Astrid S.What can we learn from clubroots:alterations in host roots and hormone homeostasis caused by Plasmodiophora brassicae [J].Eur J Plant Pathol,2008,121:291.
[18] Keen N T,Williams P H.Translocation of sugars into infected cabbage tissues during clubroot development [J].Plant Physiol,1969,44:748.
[19] Rodman J E.A taxonomic analysis of glucosinolate-producing plants,part 1:phenetics [J].Sys Bot,1991,16:598.
[20] Lovelock D A,Donald C E,Conlan X A,et al.Salicylic acid suppression of clubroot in broccoli (Brassicae oleracea;var.italica) caused by the obligate biotroph Plasmodiophora brassicae [J].Australas Plant Path,2013,42:141.
[21] David B,Astrid S,Jutta L,et al.Induction of trehalase in Arabidopsis plants infected with the trehalose-producing pathogen Plasmodiophora brassicae [J].Mol Plant Microbe Interact,2002,15:693.
[22] 况宁宁,张茹琴,鄢洪海.大白菜品种间根肿病抗性鉴定及抗性生理分析 [J].北方园艺,2012,11:140.
[23] 丁云花,简元才,余阳俊,等.我国8省市十字花科蔬菜根肿病菌生理小种的鉴定 [J].中国蔬菜,2013,1:85.
[24] 马丹丹,庞胜群,丁云花,等.甘蓝类蔬菜根肿病抗性鉴定 [J].石河子大学学报:自科版,2016,34:164.
[25] Asano T,Kageyama K,Hyakumachi M.Surface disinfestation of resting spores of Plasmodiophora brassicae used to infect hairy roots of Brassica spp.[J].Phytopathology,1999,89:314.引用本文格式:中文:蒋学飞,岳出,刘双双,等.油菜不同根肿病抗性材料的室内鉴定及相关生理分析 [J].四川大学学报:自然科学版,2019,56:573.英文:Jiang X F,Yue C,Liu S S,et al.Laboratory identification of different resistant materials with clubroot and related physiological analysis in rapeseed [J].J Sichuan Univ:Nat Sci Ed,2019,56:573.
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