78份冬小麦区试品种抗条锈病基因的分子检测
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摘要
为及时动态掌握我国小麦培育过程中对抗性基因的使用情况以及对优势生理小种的抗性水平,同时为小麦的安全生产和育种提供一定的参考资料,采用Yr5,Yr9,Yr10,Yr18及Yr26的分子标记对78份2017—2018年国家冬小麦区试品种中可能含有的抗性基因进行检测,配合使用我国当前流行生理小种CYR32和CYR34进行成株期抗性鉴定,了解我国新培育品种对条锈病的抗性情况以及抗性基因的分布。在供试的78份材料中,成株期对CYR32具有抗性的有52份;对CYR34具有抗性的有42份;同时对CYR32和CYR34具有抗性的有26份(包括来自四川麦区的全部13份材料);分别占鉴定材料的66.7%,53.8%和33.3%。分子检测结果表明,在78份鉴定材料中,可能携带Yr5,Yr9,Yr10,Yr18及Yr26的材料分别为0,21,5,1及9份,分别占鉴定材料的0%,26.9%,6.4%,1.3%及11.5%。鉴定材料中有效抗性基因的使用率低,1B/1R易位系的使用率高,成株期对当前流行小种CYR32以及新小种CYR34的抗性偏低。
For timely and dynamically grasp about the use of resistance genes and the resistance level of dominant physiological races in wheat( Triticum aestivum L.) cultivation in China,which will be helpful for safe production and facilitate in breeding programs in wheat,in this work,78 winter wheat cultivars in the national regional test during 2017—2018 were tested with molecular markers such as,Yr5,Yr9,Yr10,Yr18 and Yr26. We have also employed the current physiological races CYR32 and CYR34 to explore the resistance level in new cultivars against stripe rust and the distribution of resistance genes in these cultivars. Our results demonstrate that,among 78 cultivars examined,52 showed resistance responses to CYR32,42 were resistant to CYR34,and 26 were resistant to CYR32 and CYR34 at the adult stage,accounting for 66.7%,53.8%,33.3% of the identified materials,respectively. It is worth noting that all 13 cultivars from Sichuan wheat growing regions exhibited resistant responses against CYR32 and CYR34. Additionally,our results revealed that,among 78 identification materials,there were 0,21,5,1 and 9 cultivars possibly carrying Yr5,Yr9,Yr10,Yr18 and Yr26,which accounted for 0%,26.9%,6.4%,1.3% and 11.5% of the identification materials,respectively. In summary,our results unveiled that the utilization rate of effective resistance genes in identification materials was low,the use rate of 1 B/1 R translocation lines was high,and the resistance to current races CYR32 and CYR34 at adult stage was low.
For timely and dynamically grasp about the use of resistance genes and the resistance level of dominant physiological races in wheat( Triticum aestivum L.) cultivation in China,which will be helpful for safe production and facilitate in breeding programs in wheat,in this work,78 winter wheat cultivars in the national regional test during 2017—2018 were tested with molecular markers such as,Yr5,Yr9,Yr10,Yr18 and Yr26. We have also employed the current physiological races CYR32 and CYR34 to explore the resistance level in new cultivars against stripe rust and the distribution of resistance genes in these cultivars. Our results demonstrate that,among 78 cultivars examined,52 showed resistance responses to CYR32,42 were resistant to CYR34,and 26 were resistant to CYR32 and CYR34 at the adult stage,accounting for 66.7%,53.8%,33.3% of the identified materials,respectively. It is worth noting that all 13 cultivars from Sichuan wheat growing regions exhibited resistant responses against CYR32 and CYR34. Additionally,our results revealed that,among 78 identification materials,there were 0,21,5,1 and 9 cultivars possibly carrying Yr5,Yr9,Yr10,Yr18 and Yr26,which accounted for 0%,26.9%,6.4%,1.3% and 11.5% of the identification materials,respectively. In summary,our results unveiled that the utilization rate of effective resistance genes in identification materials was low,the use rate of 1 B/1 R translocation lines was high,and the resistance to current races CYR32 and CYR34 at adult stage was low.
引文
[1]胡小平,王保通,康振生.中国小麦条锈菌毒性变异研究进展[J].麦类作物学报,2014,34(5):709-716.
[2]CHEN W,WELLINGS C,CHEN X,et al.Wheat stripe(yellow)rust caused by Puccinia striiformis f.sp.tritici[J].Mol Plant Pathol,2014,15(5):433-446.
[3]陈万权,康振生,马占鸿,等.中国小麦条锈病综合治理理论与实践[J].中国农业科学,2013,46(20):4254-4262.
[4]陈万权,徐世昌,吴立人,等.中国小麦条锈病流行体系与持续治理研究回顾与展望[J].中国农业科学,2007,40(S1):177-183.
[5]CHEN W Q,WU L R,LIU T G,et al.Race dynamics,diversity,and virulence evolution in Puccinia striiformis f.sp.tritici,the causal agent of wheat stripe rust in China from 2003 to 2007[J].Plant Disease,2009,93(11):1093-1101.
[6]康振生,王晓杰,赵杰,等.小麦条锈菌致病性及其变异研究进展[J].中国农业科学,2015,48(17):3439-3453.
[7]汪可宁,洪锡午,吴立人,等.1951~1983年我国小麦品种抗条诱性变异分析[J].植物保护学报,1986,13(2):117-124.
[8]何中虎,兰彩霞,陈新民,等.小麦条锈病和白粉病成株抗性研究进展与展望[J].中国农业科学,2011,44(11):2193-2215.
[9]UAUY C,BREVIS J C,CHEN X,et al.High-temperature adult-plant(HTAP)stripe rust resistance gene Yr36 from Triticum turgidum ssp.dicoccoides is closely linked to the grain protein content locus Gpc-B1[J].Theor Appl Genet,2005,112(1):97-105.
[10]何中虎,夏先春,罗晶,等.国际小麦育种研究趋势分析[J].麦类作物学报,2006,26(2):154-156.
[11]刘太国,王保通,贾秋珍,等.2010-2011年度我国小麦条锈菌生理专化研究[J].麦类作物学报,2012,32(3):574-578.
[12]LIU T G,PENG Y L,CHEN W Q,et al.First detection of virulence in Puccinia striiformis f.sp.tritici in China to resistance genes Yr24(=Yr26)present in wheat cultivar Chuanmai 42[J].Plant Disease,2010,94(9):1163-1163.
[13]刘博,刘太国,章振羽,等.中国小麦条锈菌条中34号的发现及其致病特性[J].植物病理学报,2017,47(5):681-687.
[14]刘太国,章振羽,刘博,等.小麦抗条锈病基因Yr26毒性小种的发现及其对我国小麦主栽品种苗期致病性分析[J].植物病理学报,2015,45(1):41-47.
[15]何中虎,庄巧生,程顺和,等.中国小麦产业发展与科技进步[J].农学学报,2018,8(1):99-106.
[16]黄亮,刘太国,肖星芷,等.中国79个小麦品种(系)抗条锈病评价及基因分子检测[J].中国农业科学,2017,50(16):3122-3134.
[17]陈万权,刘太国,陈巨连,等.小麦抗病虫性评价技术规范.第1部分:小麦抗条锈病评价技术规范:NT/T1443.1-2007[S].2007-09-14.
[18]MARCHAL C,ZHANG J,ZHANG P,et al.BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust[J].Nat Plants,2018,4(9):662-668.
[19]FRANCIS H A,LEITCH A R,KOEBNER R M.Conversion of a RAPD-generated PCR product,containing a novel dispersed repetitive element,into a fast and robust assay for the presence of rye chromatin in wheat[J].Theor Appl Genet,1995,90(5):636-642.
[20]SHAO Y,NIU Y,ZHU L,et al.Identification of an AFLP marker linked to the stripe rust resistance gene Yr10 in wheat[J].Chin Sci Bull,2001,46(17):1466-1469.
[21]LAGUDAH E S,MCFADDEN H,SINGH R P,et al.Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat[J].Theor Appl Genet,2006,114(1):21-30.
[22]WANG C,ZHANG Y,HAN D,et al.SSR and STS markers for wheat stripe rust resistance gene Yr26[J].Euphytica,2008,159(3):359-366.
[23]赵霞,王长彪,赵兴华,等.小麦抗病相关基因聚合育种的研究进展[J].山西农业科学,2017,45(2):308-313.
[24]ROSEWARNE G M,HERRERA-FOESSEL A S,SINGH R P,et al.Quantitative trait loci of stripe rust resistance in wheat[J].Theor Appl Genet,2013,126(10):2427-2449.
[25]ZHANG P,MCINTOSH A R,HOXHA S,et al.Wheat stripe rust resistance genes Yr5 and Yr7 are allelic[J].Theor Appl Genet,2009,120(1):25-29.
[26]FENG J Y,WANG M N,CHEN X M,et al.Molecular mapping of Yr SP and its relationship with other genes for stripe rust resistance in wheat chromosome 2BL[J].Phytopathology,2015,105(9):1206-1213.
[27]李振岐,曾士迈.中国小麦锈病[M].北京:中国农业出版社,2002.
[28]李敏州,李强,巢凯翔,等.陕西省115个小麦品种(系)抗条锈病基因的分子检测[J].植物病理学报,2015,45(6):632-640.
[29]王树和,龚凯悦,初炳瑶,等.四川省100个小麦品种(系)抗条锈病基因的分子检测[J].植物病理学报,2018,48(2):195-206.
[30]王欣,张怀刚,刘宝龙,等.青海省小麦品种中Yr10和Yr15基因及其1BL/1RS易位的分子检测[J].西北植物学报,2011,31(1):57-63.
[31]于亚雄,刘丽,胡银星,等.1BL/1RS易位对小麦面筋品质的影响[J].西南农业学报,2004,17(6):685-689.
[32]刘建军,何中虎,PENA R J,等.1BL/1RS易位对小麦加工品质的影响[J].作物学报,2004,30(2):149-153.
[33]BAI B B,LIU T G,LIU B,et al.High relative parasitic fitness of G22 derivatives is associated with the epidemic potential of wheat stripe rust in China[J].Plant Disease,2017,102(3):483-487.
[34]鲁秀梅,张宁,陈劲枫,等.作物基因聚合育种的研究进展[J].分子植物育种,2017,15(4):1445-1454.
[2]CHEN W,WELLINGS C,CHEN X,et al.Wheat stripe(yellow)rust caused by Puccinia striiformis f.sp.tritici[J].Mol Plant Pathol,2014,15(5):433-446.
[3]陈万权,康振生,马占鸿,等.中国小麦条锈病综合治理理论与实践[J].中国农业科学,2013,46(20):4254-4262.
[4]陈万权,徐世昌,吴立人,等.中国小麦条锈病流行体系与持续治理研究回顾与展望[J].中国农业科学,2007,40(S1):177-183.
[5]CHEN W Q,WU L R,LIU T G,et al.Race dynamics,diversity,and virulence evolution in Puccinia striiformis f.sp.tritici,the causal agent of wheat stripe rust in China from 2003 to 2007[J].Plant Disease,2009,93(11):1093-1101.
[6]康振生,王晓杰,赵杰,等.小麦条锈菌致病性及其变异研究进展[J].中国农业科学,2015,48(17):3439-3453.
[7]汪可宁,洪锡午,吴立人,等.1951~1983年我国小麦品种抗条诱性变异分析[J].植物保护学报,1986,13(2):117-124.
[8]何中虎,兰彩霞,陈新民,等.小麦条锈病和白粉病成株抗性研究进展与展望[J].中国农业科学,2011,44(11):2193-2215.
[9]UAUY C,BREVIS J C,CHEN X,et al.High-temperature adult-plant(HTAP)stripe rust resistance gene Yr36 from Triticum turgidum ssp.dicoccoides is closely linked to the grain protein content locus Gpc-B1[J].Theor Appl Genet,2005,112(1):97-105.
[10]何中虎,夏先春,罗晶,等.国际小麦育种研究趋势分析[J].麦类作物学报,2006,26(2):154-156.
[11]刘太国,王保通,贾秋珍,等.2010-2011年度我国小麦条锈菌生理专化研究[J].麦类作物学报,2012,32(3):574-578.
[12]LIU T G,PENG Y L,CHEN W Q,et al.First detection of virulence in Puccinia striiformis f.sp.tritici in China to resistance genes Yr24(=Yr26)present in wheat cultivar Chuanmai 42[J].Plant Disease,2010,94(9):1163-1163.
[13]刘博,刘太国,章振羽,等.中国小麦条锈菌条中34号的发现及其致病特性[J].植物病理学报,2017,47(5):681-687.
[14]刘太国,章振羽,刘博,等.小麦抗条锈病基因Yr26毒性小种的发现及其对我国小麦主栽品种苗期致病性分析[J].植物病理学报,2015,45(1):41-47.
[15]何中虎,庄巧生,程顺和,等.中国小麦产业发展与科技进步[J].农学学报,2018,8(1):99-106.
[16]黄亮,刘太国,肖星芷,等.中国79个小麦品种(系)抗条锈病评价及基因分子检测[J].中国农业科学,2017,50(16):3122-3134.
[17]陈万权,刘太国,陈巨连,等.小麦抗病虫性评价技术规范.第1部分:小麦抗条锈病评价技术规范:NT/T1443.1-2007[S].2007-09-14.
[18]MARCHAL C,ZHANG J,ZHANG P,et al.BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust[J].Nat Plants,2018,4(9):662-668.
[19]FRANCIS H A,LEITCH A R,KOEBNER R M.Conversion of a RAPD-generated PCR product,containing a novel dispersed repetitive element,into a fast and robust assay for the presence of rye chromatin in wheat[J].Theor Appl Genet,1995,90(5):636-642.
[20]SHAO Y,NIU Y,ZHU L,et al.Identification of an AFLP marker linked to the stripe rust resistance gene Yr10 in wheat[J].Chin Sci Bull,2001,46(17):1466-1469.
[21]LAGUDAH E S,MCFADDEN H,SINGH R P,et al.Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat[J].Theor Appl Genet,2006,114(1):21-30.
[22]WANG C,ZHANG Y,HAN D,et al.SSR and STS markers for wheat stripe rust resistance gene Yr26[J].Euphytica,2008,159(3):359-366.
[23]赵霞,王长彪,赵兴华,等.小麦抗病相关基因聚合育种的研究进展[J].山西农业科学,2017,45(2):308-313.
[24]ROSEWARNE G M,HERRERA-FOESSEL A S,SINGH R P,et al.Quantitative trait loci of stripe rust resistance in wheat[J].Theor Appl Genet,2013,126(10):2427-2449.
[25]ZHANG P,MCINTOSH A R,HOXHA S,et al.Wheat stripe rust resistance genes Yr5 and Yr7 are allelic[J].Theor Appl Genet,2009,120(1):25-29.
[26]FENG J Y,WANG M N,CHEN X M,et al.Molecular mapping of Yr SP and its relationship with other genes for stripe rust resistance in wheat chromosome 2BL[J].Phytopathology,2015,105(9):1206-1213.
[27]李振岐,曾士迈.中国小麦锈病[M].北京:中国农业出版社,2002.
[28]李敏州,李强,巢凯翔,等.陕西省115个小麦品种(系)抗条锈病基因的分子检测[J].植物病理学报,2015,45(6):632-640.
[29]王树和,龚凯悦,初炳瑶,等.四川省100个小麦品种(系)抗条锈病基因的分子检测[J].植物病理学报,2018,48(2):195-206.
[30]王欣,张怀刚,刘宝龙,等.青海省小麦品种中Yr10和Yr15基因及其1BL/1RS易位的分子检测[J].西北植物学报,2011,31(1):57-63.
[31]于亚雄,刘丽,胡银星,等.1BL/1RS易位对小麦面筋品质的影响[J].西南农业学报,2004,17(6):685-689.
[32]刘建军,何中虎,PENA R J,等.1BL/1RS易位对小麦加工品质的影响[J].作物学报,2004,30(2):149-153.
[33]BAI B B,LIU T G,LIU B,et al.High relative parasitic fitness of G22 derivatives is associated with the epidemic potential of wheat stripe rust in China[J].Plant Disease,2017,102(3):483-487.
[34]鲁秀梅,张宁,陈劲枫,等.作物基因聚合育种的研究进展[J].分子植物育种,2017,15(4):1445-1454.
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