夏天无总碱对血管性痴呆大鼠海马神经元凋亡及认知功能的影响
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摘要
目的:研究夏天无总碱对血管性痴呆大鼠海马细胞凋亡及认知功能的影响。方法:50只大鼠分为对照组,模型组和0. 3、0. 6和1. 2 mg/kg夏天无总碱组,每组10只。模型组和夏天无总碱组构建血管性痴呆大鼠模型,0. 3、0. 6和1. 2夏天无总碱组分别灌服0. 3、0. 6和1. 2 mg/kg的夏天无总碱30 d。Morris水迷宫实验检测大鼠认知能力,用相应试剂盒分别检测大鼠海马区去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、乙酰胆碱酯酶(AchE)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,TUNEL法检测海马神经元凋亡情况,Western blot检测活化型Caspase-3(c-Caspase-3)蛋白表达水平。结果:模型组大鼠认知能力降低,NE、DA、5-HT、AchE含量下降,神经元凋亡率升高,c-Caspase-3蛋白水平升高,SOD、GSH-Px活性降低,MDA含量升高(P均<0. 05)。夏天无总碱组大鼠认知能力改善,NE、DA、5-HT、AchE含量升高,神经元细胞凋亡率降低,cCaspase-3蛋白水平降低,SOD、GSH-Px活性升高,MDA含量下降(P均<0. 05)。结论:夏天无总碱能够改善血管性痴呆大鼠认知能力,其作用机制可能与抑制氧化应激、减少海马神经元细胞凋亡、调节神经递质释放有关。
Aim: To study the effects of total alkaloids of Rhizoma Corydalis on the apoptosis of hippocampal cells and cognitive function in vascular dementia rats. Methods: A total of 50 rats were allocated into control group,model group and 0. 3,0. 6 and 1. 2 mg/kg total alkaloids of Rhizoma Corydalis groups,10 rats in each group. Vascular dementia rat models were established in model group and total alkaloids of Rhizoma Corydalis groups. The 0. 3,0. 6 and 1. 2 mg/kg total alkaloids of Rhizoma Corydalis groups were administered with corresponding concentration of total alkaloids of Rhizoma Corydalis for 30 days,respectively. Morris water maze test was used to detect the cognitive ability of rats. The levels of NE,DA,5-HT,Ach E,SOD,GSH-Px and MDA in hippocampus of rats were detected by kits. TUNEL method was used to detect apoptosis of hippocampal neurons. The expression level of c-Caspase-3 protein was detected by Western blot. Results: The cognitive ability of vascular dementia rats was significantly reduced,the levels of NE,DA,5-HT and Ach E decreased,the apoptosis rate of neurons increased,the level of c-Caspase-3 protein increased,the activity of SOD and GSH-Px decreased,and the content of MDA increased(P < 0. 05). After the use of total alkaloids of Rhizoma Corydalis,the cognitive ability of vascular dementia rats was obviously improved,the contents of NE,DA,5-HT and Ach E increased,the apoptosis rate of neurons decreased,the level of c-Caspase-3 protein decreased,the activity of SOD and GSH-Px increased,and the content of MDA decreased(P < 0. 05). Conclusion: Total alkaloids of Rhizoma Corydalis can improve the cognitive function of vascular dementia rats,and its mechanism may be related with inhibiting oxidative stress,reducing apoptosis of hippocampal neurons and regulating neurotransmitter release.
Aim: To study the effects of total alkaloids of Rhizoma Corydalis on the apoptosis of hippocampal cells and cognitive function in vascular dementia rats. Methods: A total of 50 rats were allocated into control group,model group and 0. 3,0. 6 and 1. 2 mg/kg total alkaloids of Rhizoma Corydalis groups,10 rats in each group. Vascular dementia rat models were established in model group and total alkaloids of Rhizoma Corydalis groups. The 0. 3,0. 6 and 1. 2 mg/kg total alkaloids of Rhizoma Corydalis groups were administered with corresponding concentration of total alkaloids of Rhizoma Corydalis for 30 days,respectively. Morris water maze test was used to detect the cognitive ability of rats. The levels of NE,DA,5-HT,Ach E,SOD,GSH-Px and MDA in hippocampus of rats were detected by kits. TUNEL method was used to detect apoptosis of hippocampal neurons. The expression level of c-Caspase-3 protein was detected by Western blot. Results: The cognitive ability of vascular dementia rats was significantly reduced,the levels of NE,DA,5-HT and Ach E decreased,the apoptosis rate of neurons increased,the level of c-Caspase-3 protein increased,the activity of SOD and GSH-Px decreased,and the content of MDA increased(P < 0. 05). After the use of total alkaloids of Rhizoma Corydalis,the cognitive ability of vascular dementia rats was obviously improved,the contents of NE,DA,5-HT and Ach E increased,the apoptosis rate of neurons decreased,the level of c-Caspase-3 protein decreased,the activity of SOD and GSH-Px increased,and the content of MDA decreased(P < 0. 05). Conclusion: Total alkaloids of Rhizoma Corydalis can improve the cognitive function of vascular dementia rats,and its mechanism may be related with inhibiting oxidative stress,reducing apoptosis of hippocampal neurons and regulating neurotransmitter release.
引文
[1]UENO M,CHIBA Y,MATSUMOTO K,et al.Blood-brain barrier damage in vascular dementia[J].Neuropathology,2016,36(2):115
[2]CALABRESE V,GIORDANO J,SIGNORILE A,et al.Major pathogenic mechanisms in vascular dementia:Roles of cellular stress response and hormesis in neuroprotection[J].J Neurosci Res,2016,94(12):1588
[3]潘荣斌,刘升长,候吉华,等.夏天无对神经系统疾病作用研究进展[J].亚太传统医药,2015,11(21):44
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[9]曾贵刚,张申,顾坚忠,等.运动训练对SHR/SP大鼠VD模型认知功能及海马CHAT、Ach E活性的影响[J].重庆医学,2014,43(11):1334
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[11]徐丽华,顾振纶,蒋小岗,等.夏天无总碱中抗胆碱酯酶活性成分的研究[J].药学学报,2002,37(11):902
[12]刘立新,林涌波.复方夏天无片治疗心律失常46例疗效观察[J].现代中西医结合杂志,2007,16(35):5273
[13]高健,顾振纶,王天佑.夏天无总碱对大鼠实验性脑血栓形成的影响[J].中国血液流变学杂志,2003,13(4):325
[14]周巧霞,顾振纶.夏天无的实验研究和临床应用进展[J].中国野生植物资源,2004,23(3):4
[15]ZHU Y,WU Q,LIN L.Effects of electro-acupuncture on Noxa and caspase-3 expression in hippocampal CA1 region of a vascular dementia rat model[J].Neural Regen Res,2008,3(8):826
[2]CALABRESE V,GIORDANO J,SIGNORILE A,et al.Major pathogenic mechanisms in vascular dementia:Roles of cellular stress response and hormesis in neuroprotection[J].J Neurosci Res,2016,94(12):1588
[3]潘荣斌,刘升长,候吉华,等.夏天无对神经系统疾病作用研究进展[J].亚太传统医药,2015,11(21):44
[4]马宏达,史国兵.夏天无药理作用研究进展[J].中国药房,2008,19(36):2867
[5]林海,孟庆玉,肖艳萍,等.夏天无总碱对脑缺血大鼠软脑膜微循环的影响[J].湖南中医杂志,2009,25(2):100
[6]余丽梅,于爽,龚其海,等.夏天无注射液对血管性痴呆大鼠海马血管生成素-1表达的影响[J].中成药,2006,28(6):839
[7]ZHANG LM,JIANG CX,LIU DW.Hydrogen sulfide attenuates neuronal injury induced by vascular dementia via inhibiting apoptosis in rats[J].Neurochem Res,2009,34(11):1984
[8]LIU H,ZHAO M,YANG S,et al.(2R,3S)-Pinobanksin-3-cinnamate improves cognition and reduces oxidative stress in rats with vascular dementia[J].J Nat Med,2015,69(3):358
[9]曾贵刚,张申,顾坚忠,等.运动训练对SHR/SP大鼠VD模型认知功能及海马CHAT、Ach E活性的影响[J].重庆医学,2014,43(11):1334
[10]蔺心敬,胡常林,李正国,等.血管性痴呆大鼠模型海马乙酰胆碱和血小板激活因子含量变化的动态观察[J].中风与神经疾病杂志,2002,19(4):219
[11]徐丽华,顾振纶,蒋小岗,等.夏天无总碱中抗胆碱酯酶活性成分的研究[J].药学学报,2002,37(11):902
[12]刘立新,林涌波.复方夏天无片治疗心律失常46例疗效观察[J].现代中西医结合杂志,2007,16(35):5273
[13]高健,顾振纶,王天佑.夏天无总碱对大鼠实验性脑血栓形成的影响[J].中国血液流变学杂志,2003,13(4):325
[14]周巧霞,顾振纶.夏天无的实验研究和临床应用进展[J].中国野生植物资源,2004,23(3):4
[15]ZHU Y,WU Q,LIN L.Effects of electro-acupuncture on Noxa and caspase-3 expression in hippocampal CA1 region of a vascular dementia rat model[J].Neural Regen Res,2008,3(8):826
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