重庆巫山独活最佳采收期研究
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摘要
目的比较重庆巫山独活不同采收期产量性状及蛇床子素、二氢欧山芹醇当归酸酯含量,为确定合理的采收期提供参考依据。方法采用田间小区试验法,于2015年9月5日-2016年3月3日,每20 d采收1次,共10个不同采收时期,以独活主根长、根直径、鲜重、干重、折干率为考察指标,以蛇床子素和二氢欧山芹醇当归酸酯为指标成分,应用HPLC对其进行测定,并运用主成分分析法进行综合评价。结果随着采收期的延迟,独活主根长、直径、鲜重、干重、折干率呈先增加后降低的趋势;蛇床子素含量前期不断增加,12月中旬到达最高值,随后逐渐降低;二氢欧山芹醇当归酸酯含量呈曲线变化,分别于11月下旬和2月中旬出现高峰,但差异不明显。结论重庆巫山独活单株鲜重和蛇床子素、二氢欧山芹醇当归酸酯含量与采收期有一定关系;结合鲜重和指标成分含量,其最适合的采收期应为12月中旬。本研究可为独活采收期的选择提供依据。
Objective To compare yield characteristics, contents of osthole and columbianadin of Angelicae Pubescentis Radix of different collecting time in Wushan of Chongqing; To provide references for reasonable collecting time. Methods Adopting small plot experiment in the field, taking the main root length, root diameter, fresh root weight, dry root weight and drying rate as investigation basis, collecting time from September 5th 2015 to March 3rd 2016 was divided into ten collecting time, collecting every 20 d. Taking osthole and columbianadin as the index components, the HPLC was used to determine them, and the principal component analysis method was used for comprehensive evaluation. Results Root length, root diameter, fresh root weight, dry root weight and drying rate increased first then decreased along with the collecting time. Osthole increased first, then decreased gradually after reaching a peak value in the middle of December. The change of columbianadin was a quadratic curve with the collecting time, and reached the peak at the end of November and mid-February, but there was not significant difference. Conclusion The fresh root weight and contents of osthole and columbianadin in Angelicae Pubescentis Radix has a certain relationship with the collecting time, combined with the fresh root weight and index components,the optimum collecting time of Angelicae Pubescentis Radix in Wushan of Chongqing is mid-December, which can provide theoretical basis for the choice of collecting time of Angelicae Pubescentis Radix.
Objective To compare yield characteristics, contents of osthole and columbianadin of Angelicae Pubescentis Radix of different collecting time in Wushan of Chongqing; To provide references for reasonable collecting time. Methods Adopting small plot experiment in the field, taking the main root length, root diameter, fresh root weight, dry root weight and drying rate as investigation basis, collecting time from September 5th 2015 to March 3rd 2016 was divided into ten collecting time, collecting every 20 d. Taking osthole and columbianadin as the index components, the HPLC was used to determine them, and the principal component analysis method was used for comprehensive evaluation. Results Root length, root diameter, fresh root weight, dry root weight and drying rate increased first then decreased along with the collecting time. Osthole increased first, then decreased gradually after reaching a peak value in the middle of December. The change of columbianadin was a quadratic curve with the collecting time, and reached the peak at the end of November and mid-February, but there was not significant difference. Conclusion The fresh root weight and contents of osthole and columbianadin in Angelicae Pubescentis Radix has a certain relationship with the collecting time, combined with the fresh root weight and index components,the optimum collecting time of Angelicae Pubescentis Radix in Wushan of Chongqing is mid-December, which can provide theoretical basis for the choice of collecting time of Angelicae Pubescentis Radix.
引文
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[2]李雄建,袁桂香.药用独活的资源与鉴定[J].基层中药杂志,1998,12(2):5-6.
[3]陆大忠,朱山寅.独活与香独活的鉴别[J].中国药业,2000,9(3):53-54.
[4]单峰,袁媛,郝近大,等.独活、羌活的本草源流考[J].中国中药杂志,2014,39(17):3399-3403.
[5]曹雯,李崇超.独活的本草考证及常用药对[J].陕西中医药大学学报,2018,41(1):127-130.
[6]才谦,沙明,杨松松,等.不同地区商品独活中香豆素含量比较[J].中药材,2003,26(12):857-858.
[7]张才煜,张本刚,杨秀伟.独活化学成分的研究[J].解放军药学学报,2007,23(4):241-245.
[8]丁希飞,冯煦,董云发,等.中药独活化学成分的研究[J].中药材,2008,31(4):516-518.
[9]鞠成国,王巍,姜海平.独活HPLC指纹图谱研究[J].辽宁中医药大学学报,2007,9(2):128-129.
[10]胡太德,陈绍成,谭君.重庆道地药材独活与全国其他产区独活主要药效成分含量对比研究[J].中国药房,2015,26(33):4711-4713.
[11]张志美,张春华,沈志强,等.中药独活药理研究[J].中兽医学杂志,2008(6):42-43.
[12]龚高柏,吴红玲.独活常见药对的临床应用[J].现代中西医结合杂志,2005,20(14):2653-2654.
[13]严宜昌,廖玮,李晓莉,等.独活栽培中的主要因素及种植方案优选试验研究[J].湖北中医杂志,2012,34(2):65-66.
[14]王显安,胡榜文,马超,等.镇坪县独活施肥效果对比试验[J].现代农业科技,2017(23):51,53
[15]王康才,陈暄,唐晓清,等.独活种子发芽特性研究[J].中草药,2005,36(4):595-597.
[16]田新村.中药材采收期研究概述[J].中药材,1989,12(8):40-43.
[17]贾树帜,赵伟群,王桂嫒,等.桔梗最佳采收期实验研究[J].中医药学报,1997,25(4):35-36.
[18]董娟娥,梁宗锁.植物次生代谢物积累量影响因素分析[J].西北植物学报,2004,24(10):1979-1983.
[19]吴洋,王慧,房敏峰,等.环境因子对药用植物药材形成的影响研究进展[J].天然产物研究与开发,2013,25(4):416-420,429.
[20]龙全江.中药材加工学[M]:北京:中国中医药出版社,2006:18.
[21]段金廒,严辉,宿树兰,等.药材适宜采收期综合评价模式的建立与实践[J].中草药,2010,41(11):1755-1760.