铝胁迫对野葛叶绿素含量和叶绿素荧光参数的影响
|
摘要
本研究采用铝浇灌野葛扦插苗的方法,探索铝胁迫对野葛叶绿素含量、荧光参数和净光合速率的影响。研究结果表明,随着铝胁迫程度的增加,野葛叶绿素含量均逐渐降低,Fo值逐渐增大,Fm、Fv/Fm值和Pn均逐渐降低;低浓度铝胁迫对野葛叶绿素含量、荧光参数和净光合速率均影响不大,高浓度铝胁迫下各指标均受到显著影响。250μmol/L的铝胁迫下Fo值显著(p<0.05)高于CK,250μmol/L的铝胁迫下Fm值显著(p<0.05)低于CK,200μmol/L的铝胁迫下Fv/Fm值显著(p<0.05)高于CK。铝胁迫显著(p<0.05)抑制野葛叶片叶绿素含量的浓度为100μmol/L,显著(p<0.05)抑制野葛净光合速率的浓度为150μmol/L。
The impact of aluminum stress on chlorophyll content, fluorescence parameters and net photosynthetic rate of kudzu was studied by the method of aluminum watering kudzu cuttings in this study. The results showed that with the increase of aluminum concentration, the chlorophyll content decreased, Fo value increased, and Fm, Fv/Fm values and Pn were all gradually decreased. Low concentration aluminum stress had little effect on chlorophyll content, fluorescence parameters and net photosynthetic rate of Pueraria lobata, while all indexes were significantly affected under high concentration aluminum stress. The value of Fo was significantly higher than that of CK under aluminum stress of 250 μmol/L, while the value of Fm was significantly lower than CK under 250 μmol/L aluminum stress and the value of Fv/Fm was significantly lower than CK under 200 μmol/L aluminum stress. Aluminum stress significantly(p<0.05) inhibited the chlorophyll content of Pueraria lobata leaves at a concentration of 100 μmol/L, and significantly inhibited the net photosynthetic rate of Pueraria lobata leaves at a concentration of 150 μmol/L(p<0.05).
The impact of aluminum stress on chlorophyll content, fluorescence parameters and net photosynthetic rate of kudzu was studied by the method of aluminum watering kudzu cuttings in this study. The results showed that with the increase of aluminum concentration, the chlorophyll content decreased, Fo value increased, and Fm, Fv/Fm values and Pn were all gradually decreased. Low concentration aluminum stress had little effect on chlorophyll content, fluorescence parameters and net photosynthetic rate of Pueraria lobata, while all indexes were significantly affected under high concentration aluminum stress. The value of Fo was significantly higher than that of CK under aluminum stress of 250 μmol/L, while the value of Fm was significantly lower than CK under 250 μmol/L aluminum stress and the value of Fv/Fm was significantly lower than CK under 200 μmol/L aluminum stress. Aluminum stress significantly(p<0.05) inhibited the chlorophyll content of Pueraria lobata leaves at a concentration of 100 μmol/L, and significantly inhibited the net photosynthetic rate of Pueraria lobata leaves at a concentration of 150 μmol/L(p<0.05).
引文
Chen X.,Hu X.H.,Xiao Y.A.,Lu Y.D.,and Li Y.,2011,Comparison of growth and chlorophyll fluorescence characteristics between Plantago virginica and P.asiatica under aluminum stress,Zhiwu Yanjiu(Bulletin of Botanical Research),31(6):680-685(陈香,胡雪华,肖宜安,陆耀东,李蕴,2011,铝胁迫下北美车前和车前生长及叶绿素荧光特性的比较研究,植物研究,31(6):680-685)
Lei S.X.,2005,Effects of aluminum(Al)stress on physiological characteristics of soybean root and action of alleviating Altoxicity by boron and magnesium,Thesis for M.S.,Jilin University,Supervisor:Yang Z.M.,pp.2(类淑霞,2005,铝胁迫对大豆根系生理特性的影响及镁,硼对铝毒的缓解效应,硕士学位论文,吉林大学,导师:杨振明,pp.2)
Li B.F.,Cao M.J.,Liu B.,and Wu X.D.,2006,Study on maize aluminum tolerance mechanism,Yumi Kexue(Maize Sciences),14(1):111-113,119(李必富,曹敏建,刘蓓,吴晓东,2006,玉米耐铝机制研究初报,玉米科学,14(1):111-113,119)
Li T.T.,Gao Y.F.,Ma X.,Chen Y.F.,Wang Y.,and Ma J.B.,2016,Effects of exogenous brassinosteroid on photosynthesis of three spesies of Populus under drought,salt and copper stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(1):218-226(李涛涛,高永峰,马瑄,陈永富,王阳,马金彪,2016,外源油菜素内酯对三种杨树在干旱,盐和铜胁迫下光合生理的影响,基因组学与应用生物学,35(1):218-226)
Liu J.L.,Xia M.Z.,Luo Z.T.,Yuan Y.,Li L.N.,and Yang H.,2004,Germplasm resource of Pueraria DC.and its gardening application in China,Linye Keji(Forestry Science and Technology),29(4):60-62(刘建林,夏明忠,罗支铁,袁颖,李丽娜,杨红,2004,中国葛属种质资源及在园林绿化中的应用,林业科技,29(4):60-62)
Liu Q.,Zheng S.J.,and Lin X.Y.,2004,Plant physiological and molecular biological mechanism in response to aluminium toxicity,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),15(9):1641-1649(刘强,郑绍建,林咸永,2004,植物适应铝毒胁迫的生理及分子生物学机理,应用生态学报,15(9):1641-1649)
Liu X.L.,Xu C.,Xu K.Z.,Cui J.J.,An J.H.,Ling F.L.,Zhang Z.A.,and Wu Z.H.,2014,Effects on characteristics of photosynthesis and chlorophyll fluorescence of rice under salt stress,Zuowu Zazhi(Crops),(2):88-92(刘晓龙,徐晨,徐克章,崔菁菁,安久海,凌凤楼,张治安,武志海,2014,盐胁迫对水稻叶片光合作用和叶绿素荧光特性的影响,作物杂志,(2):88-92)
Wang Z.Y.,Liu P.,Li J.S.,Wu H.F.,Liu Y.,and Lu B.X.,2011,Effects of exogenous organic acids on root morphology and chlorophyll fluorescence characteristics of oilseed rape under aluminum stress,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),27(4):756-762(王志颖,刘鹏,李锦山,吴惠芳,刘莹,芦伯鑫,2011,铝胁迫下外源有机酸对油菜根系形态及叶绿素荧光特性的影响,江苏农业学报,27(4):756-762)
Wang Z.Y.,Liu P.,Li J.S.,Wu H.F.,Zheng Y.,Wang Y.T.,and Xu Y.,2010,Effect of Al stress on growth and chlorophyll fluorescence parameters,metabolizing enzymes of oil rape,Zhejiang Shifan Daxue Xuebao(Journal of Zhejiang Normal University),33(4):452-458(王志颖,刘鹏,李锦山,吴惠芳,郑赟,汪艳婷,徐艳,2010,铝胁迫对油菜生长及叶绿素荧光参数,代谢酶的影响,浙江师范大学学报,33(4):452-458)
Yang Y.X.,Yang P.Q.,Xu T.,Yu Y.X.,and Li X.F.,2009,Al-detoxification by Si in Stylosanthes,Jiyingzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(1):57-61(杨永先,杨培权,许涛,玉永雄,黎晓峰,2009,硅对柱花草铝毒的解毒作用,基因组学与应用生物学,28(1):57-61)
Lei S.X.,2005,Effects of aluminum(Al)stress on physiological characteristics of soybean root and action of alleviating Altoxicity by boron and magnesium,Thesis for M.S.,Jilin University,Supervisor:Yang Z.M.,pp.2(类淑霞,2005,铝胁迫对大豆根系生理特性的影响及镁,硼对铝毒的缓解效应,硕士学位论文,吉林大学,导师:杨振明,pp.2)
Li B.F.,Cao M.J.,Liu B.,and Wu X.D.,2006,Study on maize aluminum tolerance mechanism,Yumi Kexue(Maize Sciences),14(1):111-113,119(李必富,曹敏建,刘蓓,吴晓东,2006,玉米耐铝机制研究初报,玉米科学,14(1):111-113,119)
Li T.T.,Gao Y.F.,Ma X.,Chen Y.F.,Wang Y.,and Ma J.B.,2016,Effects of exogenous brassinosteroid on photosynthesis of three spesies of Populus under drought,salt and copper stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(1):218-226(李涛涛,高永峰,马瑄,陈永富,王阳,马金彪,2016,外源油菜素内酯对三种杨树在干旱,盐和铜胁迫下光合生理的影响,基因组学与应用生物学,35(1):218-226)
Liu J.L.,Xia M.Z.,Luo Z.T.,Yuan Y.,Li L.N.,and Yang H.,2004,Germplasm resource of Pueraria DC.and its gardening application in China,Linye Keji(Forestry Science and Technology),29(4):60-62(刘建林,夏明忠,罗支铁,袁颖,李丽娜,杨红,2004,中国葛属种质资源及在园林绿化中的应用,林业科技,29(4):60-62)
Liu Q.,Zheng S.J.,and Lin X.Y.,2004,Plant physiological and molecular biological mechanism in response to aluminium toxicity,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),15(9):1641-1649(刘强,郑绍建,林咸永,2004,植物适应铝毒胁迫的生理及分子生物学机理,应用生态学报,15(9):1641-1649)
Liu X.L.,Xu C.,Xu K.Z.,Cui J.J.,An J.H.,Ling F.L.,Zhang Z.A.,and Wu Z.H.,2014,Effects on characteristics of photosynthesis and chlorophyll fluorescence of rice under salt stress,Zuowu Zazhi(Crops),(2):88-92(刘晓龙,徐晨,徐克章,崔菁菁,安久海,凌凤楼,张治安,武志海,2014,盐胁迫对水稻叶片光合作用和叶绿素荧光特性的影响,作物杂志,(2):88-92)
Wang Z.Y.,Liu P.,Li J.S.,Wu H.F.,Liu Y.,and Lu B.X.,2011,Effects of exogenous organic acids on root morphology and chlorophyll fluorescence characteristics of oilseed rape under aluminum stress,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),27(4):756-762(王志颖,刘鹏,李锦山,吴惠芳,刘莹,芦伯鑫,2011,铝胁迫下外源有机酸对油菜根系形态及叶绿素荧光特性的影响,江苏农业学报,27(4):756-762)
Wang Z.Y.,Liu P.,Li J.S.,Wu H.F.,Zheng Y.,Wang Y.T.,and Xu Y.,2010,Effect of Al stress on growth and chlorophyll fluorescence parameters,metabolizing enzymes of oil rape,Zhejiang Shifan Daxue Xuebao(Journal of Zhejiang Normal University),33(4):452-458(王志颖,刘鹏,李锦山,吴惠芳,郑赟,汪艳婷,徐艳,2010,铝胁迫对油菜生长及叶绿素荧光参数,代谢酶的影响,浙江师范大学学报,33(4):452-458)
Yang Y.X.,Yang P.Q.,Xu T.,Yu Y.X.,and Li X.F.,2009,Al-detoxification by Si in Stylosanthes,Jiyingzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(1):57-61(杨永先,杨培权,许涛,玉永雄,黎晓峰,2009,硅对柱花草铝毒的解毒作用,基因组学与应用生物学,28(1):57-61)
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |