水库底泥氮释放及其好氧微生物脱氮研究
|
摘要
以丹江口水库为例,考察水库底泥在不同温度、扰动和曝气等条件下,总氮、硝氮、氨氮和亚硝氮的释放规律。设置模拟反应器,探究高效好氧脱氮微生物强化消除水库底泥内源氮污染的效果,并运用高通量测序技术,分析高效好氧脱氮微生物对底泥微生物群落结构的影响。结果表明,温度升高会减少氨氮的释放,增加硝氮和亚硝氮的积累;水体扰动会加速底泥中氮素释放,且上覆水中的氮素释放累积量与扰动速度成正比;溶解氧对底泥氮素释放有显著影响,曝气处理可以明显地降低底泥中总氮和硝氮的释放及其在水体中的累积。在反应器中底泥–上覆水界面投加高效好氧脱氮微生物Pseudomonas stutzeri (PCN-1)后,反应器内各种形态的氮素都出现先上升、后下降的趋势;在反应器运行的第65天,底泥释放的总氮和硝氮的去除率分别高达75.87%和79.96%,底泥内源氮污染得到有效的控制。对比投加菌株前后的微生物群落结构,发现底泥中Proteobacteria,Bacteroidetes和Spirochaetes的相对丰度明显增加,PCN-1强化脱氮处理能够改变底泥的微生物群落结构。
This study focuses on the Danjiangkou reservoir, and investigates the release regulation of total nitrogen, nitrate, nitrite and ammonia from sediments as a function of temperature, perturbation and aeration conditions. Moreover, a simulation reactor was set up to explore the elimination of endogenous nitrogen pollution through high-efficient aerobic denitrification microorganism augmentation. Effects of high-efficient aerobic denitrification microorganisms on the microbial community structure in the sediments was also evaluated by means of high-throughput sequencing technology. The results indicated that increasing temperature could promote the release of nitrate and nitrite from sediments, while inhibiting the release of ammonium. Disturbances of water was beneficial to nitrogen release from sediments, and the nitrogen amount accumulated in the overlying water was proportional to the agitation speed. Concentrations of dissolved oxygen had great effects on the nitrogen release from sediments. It was found that the aeration treatment significantly reduced the release of total nitrogen and nitrite from sediments, and the subsequent accumulation in water. After addition of the a high-efficient aerobic denitrification bacteria Pseudomonas stutzeri(PCN-1) into the simulation reactor, concentrations of all the forms of nitrogen in the reactor increased at first and then decreased. On the 65 th day of the experiment, removal rates of total nitrogen and nitrate released from sediments were as high as 75.87% and 79.96% respectively, suggesting effective control of the endogenous nitrogen. The relative abundance of Proteobacteria, Bacteroidetes and Spirochaetes in sediments was significantly increased after PCN-1 addition, so the microbial community structure in the sediments was changed by microbial augmentation treatment with PCN-1 as well.
This study focuses on the Danjiangkou reservoir, and investigates the release regulation of total nitrogen, nitrate, nitrite and ammonia from sediments as a function of temperature, perturbation and aeration conditions. Moreover, a simulation reactor was set up to explore the elimination of endogenous nitrogen pollution through high-efficient aerobic denitrification microorganism augmentation. Effects of high-efficient aerobic denitrification microorganisms on the microbial community structure in the sediments was also evaluated by means of high-throughput sequencing technology. The results indicated that increasing temperature could promote the release of nitrate and nitrite from sediments, while inhibiting the release of ammonium. Disturbances of water was beneficial to nitrogen release from sediments, and the nitrogen amount accumulated in the overlying water was proportional to the agitation speed. Concentrations of dissolved oxygen had great effects on the nitrogen release from sediments. It was found that the aeration treatment significantly reduced the release of total nitrogen and nitrite from sediments, and the subsequent accumulation in water. After addition of the a high-efficient aerobic denitrification bacteria Pseudomonas stutzeri(PCN-1) into the simulation reactor, concentrations of all the forms of nitrogen in the reactor increased at first and then decreased. On the 65 th day of the experiment, removal rates of total nitrogen and nitrate released from sediments were as high as 75.87% and 79.96% respectively, suggesting effective control of the endogenous nitrogen. The relative abundance of Proteobacteria, Bacteroidetes and Spirochaetes in sediments was significantly increased after PCN-1 addition, so the microbial community structure in the sediments was changed by microbial augmentation treatment with PCN-1 as well.
引文
[1]Zhao H,Li Z,Wang S,et al.Features and influencing factors of nitrogen and phosphorus diffusive fluxes at the sediment-water interface of Erhai Lake.Environmental Science&Pollution Research International,2018,25(2):1-10
[2]Brito D,Ramos T B,Gon?alves M C,et al.Integrated modelling for water quality management in a eutrophic reservoir in south-eastern Portugal.Environmental Earth Sciences,2018,77(2):40-60
[3]Cavalcante H,Araujo F,Noyma N P,et al.Phosphorus fractionation in sediments of tropical semiarid reservoirs.Science of the Total Environment,2018,619:1022-1029
[4]Nowlin W H,Evarts J L,Vanni M J.Release rates and potential fates of nitrogen and phosphorus from sediments in a eutrophic reservoir.Freshwater Biology,2005,50:301-302
[5]Li T,Chu C,Zhang Y,et al.Contrasting eutrophication risks and countermeasures in different water bodies:assessments to support targeted watershed management.International Journal of Environmental Research&Public Health,2017,14(7):695-713
[6]Mi C X.Study on pollution status of nitrogen and phosphorus in typical reservoirs in Liaoning Province.Advanced Materials Research,2013,777:420-423
[7]Marselina M,Burhanudin M.Trophic status assessment of saguling reservoir,Upper Citarum Basin,Indonesia.Air Soil&Water Research,2017,10(38):1-8
[8]Cunha D G F,Lima V F D M,Néri A M,et al.Uptake rates of ammonium and nitrate by phytoplankton communities in two eutrophic tropical reservoirs.International Review of Hydrobiology,2017,102(2):125-134
[9]He J,Deng W,Chen C,et al.Ammonia nitrogen adsorption and release characteristics of surface sediments in Dianchi Lake,China.Environmental Earth Sciences,2015,74(5):3917-3927
[10]Liang Z,Liu Z,Zhen S,et al.Phosphorus speciation and effects of environmental factors on release of phosphorus from sediments obtained from Taihu Lake,Tien Lake,and East Lake.Toxicological&Environmental Chemistry Reviews,2015,97(3/4):335-348
[11]Wang J,Wang S,Jin X,et al.Ammonium release characteristics of the sediments from the shallow lakes in the middle and lower reaches of Yangtze River region,China.Environmental Geology,2008,55(1):37-45
[12]Fukuhara H,Sakamoto M.Enhancement of inorganic nitrogen and phosphate release from lake sediment by tubificid worms and chironomid larvae.Copenhagen.1987,3(48):312-320
[13]Li Y,Zhou Y,Zhou Z,et al.Effects of sustainedrelease composite on the oxygen levels and sediment phosphorus fractions of an urban river in Shanghai.Environmental Technology,2014,35:2176-2182
[14]Kang M,Peng S,Tian Y,et al.Effects of dissolved oxygen and nutrient loading on phosphorus fluxes at the sediment-water interface in the Hai River Estuary,China.Marine Pollution Bulletin,2018,130:132-139
[15]Shi X,Ma W X,Li X,et al.Typical reservoir pollution source analysis//Huang T,Cheng Y,Cong H,et al.Water Pollution and Water Quality Control of Selected Chinese Reservoir Basins.Cham:Springer,2016:95-130
[16]薄涛,季民.内源污染控制技术研究进展.生态环境学报,2017,26(3):514-521
[17]柴蓓蓓.水源水库沉积物多相界面污染物迁移转化与污染控制研究[D].西安:西安建筑科技大学,2012
[18]Liu T,Liu S,Zheng M,et al.Performance assessment of full-scale wastewater treatment plants based on seasonal variability of microbial communities via highthroughput sequencing.PLOS ONE,2016,11(4):1-15
[19]李辉,潘学军,史丽琼,等.湖泊内源氮磷污染分析方法及特征研究进展.环境化学,2011,30(1):281-292
[20]陶玉炎,耿金菊,王荣俊,等.环境条件变化对河流沉积物“三氮”释放的影响.环境科学与技术,2013,36(6L):41-44
[21]孙志高,刘景双.湿地土壤的硝化-反硝化作用及影响因素.土壤通报,2008,39(6):1462-1467
[22]余荣台.城市内河沉积物硝态氮释放行为的模拟研究.环境工程学报,2014,8(7):2870-2874
[23]罗玉兰.城市内河沉积物营养盐污染特性及释放规律研究[D].南京:河海大学,2007
[24]姜永生,李晓晨,邢友华,等.扰动对东平湖表层沉积物中氮磷释放的影响.环境科学与技术,2010,33(8):41-44
[25]代政,祁艳丽,唐永杰,等.上覆水环境因子对滨海水库沉积物氮磷释放的影响.环境科学研究,2016,29(12):1766-1772
[26]张亚楠.东昌湖表层沉积物氮赋存形态及其释放研究[D].青岛:中国海洋大学,2013
[27]周成.湖泊受污底泥营养物释放机理及水质影响试验研究[D].武汉:武汉大学,2017
[28]魏全源.微生物对水库沉积物氮释放的影响研究[D].西安:西安建筑科技大学,2009
[29]李薇.溶解氧水平对富营养化水体底泥氮磷转化影响的研究[D].南京:南京理工大学,2014
[30]黄廷林,杨凤英,柴蓓蓓,等.水源水库污染底泥不同修复方法脱氮效果对比实验研究.中国环境科学,2012,32(11):2032-2038
[31]黄建军.城市河道底泥营养盐释放及化学修复研究[D].天津:天津大学,2009
[32]杨浩,张国珍,杨晓妮,等.16S rRNA高通量测序研究集雨窖水中微生物群落结构及多样性.环境科学,2017,38(4):1704-1716
[33]林燕,张焕杰,刘曦,等.固定反硝化菌强化人工湿地处理低污染水研究.农业环境科学学报,2016,35(11):2154-2162
[34]Chen Q,Ni J,Ma T,et al.Bioaugmentation treatment of municipal wastewater with heterotrophic-aerobic nitrogen removal bacteria in a pilot-scale SBR.Bioresource Technology,2015,183:25-32
[35]范军辉,郝瑞霞,朱晓霞,等.温度对SCSC-S/Fe复合系统脱氮除磷及微生物群落特性的影响.环境科学,2017,38(5):2012-2020
[36]Zheng M,He D,Ma T,et al.Reducing NO and N2Oemission during aerobic denitrification by newly isolated Pseudomonas stutzeri PCN-1.Bioresource Technology,2014,162:80-88
[2]Brito D,Ramos T B,Gon?alves M C,et al.Integrated modelling for water quality management in a eutrophic reservoir in south-eastern Portugal.Environmental Earth Sciences,2018,77(2):40-60
[3]Cavalcante H,Araujo F,Noyma N P,et al.Phosphorus fractionation in sediments of tropical semiarid reservoirs.Science of the Total Environment,2018,619:1022-1029
[4]Nowlin W H,Evarts J L,Vanni M J.Release rates and potential fates of nitrogen and phosphorus from sediments in a eutrophic reservoir.Freshwater Biology,2005,50:301-302
[5]Li T,Chu C,Zhang Y,et al.Contrasting eutrophication risks and countermeasures in different water bodies:assessments to support targeted watershed management.International Journal of Environmental Research&Public Health,2017,14(7):695-713
[6]Mi C X.Study on pollution status of nitrogen and phosphorus in typical reservoirs in Liaoning Province.Advanced Materials Research,2013,777:420-423
[7]Marselina M,Burhanudin M.Trophic status assessment of saguling reservoir,Upper Citarum Basin,Indonesia.Air Soil&Water Research,2017,10(38):1-8
[8]Cunha D G F,Lima V F D M,Néri A M,et al.Uptake rates of ammonium and nitrate by phytoplankton communities in two eutrophic tropical reservoirs.International Review of Hydrobiology,2017,102(2):125-134
[9]He J,Deng W,Chen C,et al.Ammonia nitrogen adsorption and release characteristics of surface sediments in Dianchi Lake,China.Environmental Earth Sciences,2015,74(5):3917-3927
[10]Liang Z,Liu Z,Zhen S,et al.Phosphorus speciation and effects of environmental factors on release of phosphorus from sediments obtained from Taihu Lake,Tien Lake,and East Lake.Toxicological&Environmental Chemistry Reviews,2015,97(3/4):335-348
[11]Wang J,Wang S,Jin X,et al.Ammonium release characteristics of the sediments from the shallow lakes in the middle and lower reaches of Yangtze River region,China.Environmental Geology,2008,55(1):37-45
[12]Fukuhara H,Sakamoto M.Enhancement of inorganic nitrogen and phosphate release from lake sediment by tubificid worms and chironomid larvae.Copenhagen.1987,3(48):312-320
[13]Li Y,Zhou Y,Zhou Z,et al.Effects of sustainedrelease composite on the oxygen levels and sediment phosphorus fractions of an urban river in Shanghai.Environmental Technology,2014,35:2176-2182
[14]Kang M,Peng S,Tian Y,et al.Effects of dissolved oxygen and nutrient loading on phosphorus fluxes at the sediment-water interface in the Hai River Estuary,China.Marine Pollution Bulletin,2018,130:132-139
[15]Shi X,Ma W X,Li X,et al.Typical reservoir pollution source analysis//Huang T,Cheng Y,Cong H,et al.Water Pollution and Water Quality Control of Selected Chinese Reservoir Basins.Cham:Springer,2016:95-130
[16]薄涛,季民.内源污染控制技术研究进展.生态环境学报,2017,26(3):514-521
[17]柴蓓蓓.水源水库沉积物多相界面污染物迁移转化与污染控制研究[D].西安:西安建筑科技大学,2012
[18]Liu T,Liu S,Zheng M,et al.Performance assessment of full-scale wastewater treatment plants based on seasonal variability of microbial communities via highthroughput sequencing.PLOS ONE,2016,11(4):1-15
[19]李辉,潘学军,史丽琼,等.湖泊内源氮磷污染分析方法及特征研究进展.环境化学,2011,30(1):281-292
[20]陶玉炎,耿金菊,王荣俊,等.环境条件变化对河流沉积物“三氮”释放的影响.环境科学与技术,2013,36(6L):41-44
[21]孙志高,刘景双.湿地土壤的硝化-反硝化作用及影响因素.土壤通报,2008,39(6):1462-1467
[22]余荣台.城市内河沉积物硝态氮释放行为的模拟研究.环境工程学报,2014,8(7):2870-2874
[23]罗玉兰.城市内河沉积物营养盐污染特性及释放规律研究[D].南京:河海大学,2007
[24]姜永生,李晓晨,邢友华,等.扰动对东平湖表层沉积物中氮磷释放的影响.环境科学与技术,2010,33(8):41-44
[25]代政,祁艳丽,唐永杰,等.上覆水环境因子对滨海水库沉积物氮磷释放的影响.环境科学研究,2016,29(12):1766-1772
[26]张亚楠.东昌湖表层沉积物氮赋存形态及其释放研究[D].青岛:中国海洋大学,2013
[27]周成.湖泊受污底泥营养物释放机理及水质影响试验研究[D].武汉:武汉大学,2017
[28]魏全源.微生物对水库沉积物氮释放的影响研究[D].西安:西安建筑科技大学,2009
[29]李薇.溶解氧水平对富营养化水体底泥氮磷转化影响的研究[D].南京:南京理工大学,2014
[30]黄廷林,杨凤英,柴蓓蓓,等.水源水库污染底泥不同修复方法脱氮效果对比实验研究.中国环境科学,2012,32(11):2032-2038
[31]黄建军.城市河道底泥营养盐释放及化学修复研究[D].天津:天津大学,2009
[32]杨浩,张国珍,杨晓妮,等.16S rRNA高通量测序研究集雨窖水中微生物群落结构及多样性.环境科学,2017,38(4):1704-1716
[33]林燕,张焕杰,刘曦,等.固定反硝化菌强化人工湿地处理低污染水研究.农业环境科学学报,2016,35(11):2154-2162
[34]Chen Q,Ni J,Ma T,et al.Bioaugmentation treatment of municipal wastewater with heterotrophic-aerobic nitrogen removal bacteria in a pilot-scale SBR.Bioresource Technology,2015,183:25-32
[35]范军辉,郝瑞霞,朱晓霞,等.温度对SCSC-S/Fe复合系统脱氮除磷及微生物群落特性的影响.环境科学,2017,38(5):2012-2020
[36]Zheng M,He D,Ma T,et al.Reducing NO and N2Oemission during aerobic denitrification by newly isolated Pseudomonas stutzeri PCN-1.Bioresource Technology,2014,162:80-88
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |