草鱼在摄食高能饲料后血清生化指标的动态变化
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摘要
为研究草鱼(Ctenopharyngodon idella)在摄食高能饲料后血清生化指标的动态变化,选择均重为(55.0±2.5) g的健康草鱼320尾,随机分成4组,每组4个重复,分别以3组高能纯化饲料(分别以额外的蛋白质、脂肪、糊精供能,高能饲料组较对照组总能量提高11%)及对照组饲料喂养草鱼11周后,将鱼体禁食48h后采样(对照),再饱食投喂,在摄食后的2h、8h和24h取样,测定实验鱼8种血清生化指标,并对采样时间点(T)和不同饲料(D)进行双因素方差分析。为确保实验结果的准确性,需要保证不同处理组的采样时间同步性,即均在餐后同一时间点采样。结果显示:除血清总胆固醇(TCHO)含量外,血清碱性磷酸酶(ALP)、谷草转氨酶(AST)、谷丙转氨酶(ALT)活性,以及血清甘油三酯(TAG)、葡萄糖(GLU)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDLC)含量均受到采样时间点(T)即餐后时间的显著影响,饲料(D)对上述8个指标亦均有显著影响,且两者存在交互作用。草鱼血清GLU含量和血清AST活性均在餐后2h达到峰值,血清ALT和ALP活性以及LDLC含量均在餐后24h达到峰值,血清TAG含量在餐后8h达到峰值,而血清TCHO和HDLC含量则无明显峰值。在餐后24h, 3个高能组的ALT活性和GLU含量较对照组显著升高,血清AST和ALP活性以及TAG、TCHO和HDLC含量较对照组无显著差异。上述结果表明,草鱼血清生化指标受到餐后采样时间点和饲料的显著影响。
To investigate effects of high energy diets on serum biochemical indices of grass carp(Ctenopharyngodon idella), grass carp [N= 320, average initial body weight=(55.0±2.5) g] were randomly assigned into 4 groups with 4 replicates for each group(N=20 fish/replicate) to fed three times daily to satiation with the control diet(CON) and three high energy diets for 11 weeks. The 3 high energy diets were 11% increased protein, 11% increased fat or 11% increased Carbohydrate compared to the control diet, respectively. The fish were fasted for 48 h and then refed a single meal for 0, 2, 8, or 24 hours before collection for analyzing eight serum biochemical indices. Statistical analysis using two-way ANOVA showed that seven serum biochemical indices except total cholesterol(TCHO) were significantly affected by refeeding time, and all 8 biochemical indices were significantly affected by the different diets. Peak values of glucose(GLU) contents and aspartate transaminase(AST) activities presented at 2 h after refeeding, and peak values of alanine transaminase(ALT), alkaline phosphatase(ALP) activities and low density lipoprotein-cholesterol(LDLC)contents appeared at 24 h after refeeding. The peak value of triacylglycerol(TAG) content occurred at 8 h after refeeding, but there was no obvious peak value for serum TCHO or high density lipoprotein-cholesterol(HDLC) contents. At24 h after refeeding, compared with the CON diet, the higher energy diets had higher GLU contents and ALT activities but with similar activities of AST and ALP, and the contents of TAG, TCHO and HDLC. Our results demonstrated that both refeeding time and high energy diets regulated the serum biochemical indices, indicating that it is necessary to make sampling time synchronize in different groups for the accuracy of serum biochemical indices.
To investigate effects of high energy diets on serum biochemical indices of grass carp(Ctenopharyngodon idella), grass carp [N= 320, average initial body weight=(55.0±2.5) g] were randomly assigned into 4 groups with 4 replicates for each group(N=20 fish/replicate) to fed three times daily to satiation with the control diet(CON) and three high energy diets for 11 weeks. The 3 high energy diets were 11% increased protein, 11% increased fat or 11% increased Carbohydrate compared to the control diet, respectively. The fish were fasted for 48 h and then refed a single meal for 0, 2, 8, or 24 hours before collection for analyzing eight serum biochemical indices. Statistical analysis using two-way ANOVA showed that seven serum biochemical indices except total cholesterol(TCHO) were significantly affected by refeeding time, and all 8 biochemical indices were significantly affected by the different diets. Peak values of glucose(GLU) contents and aspartate transaminase(AST) activities presented at 2 h after refeeding, and peak values of alanine transaminase(ALT), alkaline phosphatase(ALP) activities and low density lipoprotein-cholesterol(LDLC)contents appeared at 24 h after refeeding. The peak value of triacylglycerol(TAG) content occurred at 8 h after refeeding, but there was no obvious peak value for serum TCHO or high density lipoprotein-cholesterol(HDLC) contents. At24 h after refeeding, compared with the CON diet, the higher energy diets had higher GLU contents and ALT activities but with similar activities of AST and ALP, and the contents of TAG, TCHO and HDLC. Our results demonstrated that both refeeding time and high energy diets regulated the serum biochemical indices, indicating that it is necessary to make sampling time synchronize in different groups for the accuracy of serum biochemical indices.
引文
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