脑瘫伴癫痫儿童肠道菌群变化
|
摘要
目的探讨脑瘫伴癫痫儿童肠道菌群的变化。方法选择龙岗区社会福利中心诊断为脑瘫伴癫痫的31例患儿作为疾病组;另外选取同龄健康儿童30例作为健康组。留取受试者的粪便标本,提取总DNA,并对16S rRNA基因的V3-V4可变区进行扩增,并在Illumina MiSeq平台进行高通量测序。结果与健康儿童相比,疾病组儿童肠道内微生物多样性出现明显差异。在门水平优势菌群中,疾病组儿童放线菌的相对丰度明显高于健康组(32.87%vs 3.56%),而拟杆菌的相对丰度明显低于健康组(22.66%vs 53.59%)。在属水平优势菌群中,疾病组儿童双歧杆菌属、副拟杆菌属的相对丰度均明显高于健康组(26.94%vs 2.61%,5.72%vs 1.90%),其中肠杆菌属的相对丰度达6.00%,而拟杆菌属、粪杆菌属的相对丰度明显低于健康组(11.38%vs 45.16%,0.63%vs 12.78%),普雷沃菌属的相对丰度也低于健康组(3.11%vs 5.30%)。结论与同龄健康儿童相比,脑瘫伴癫痫儿童肠道菌群改变明显,菌群门水平和属水平均有明显差异。
Objective To observe the changes of intestinal flora in children with cerebral palsy accompanied by epilepsy. Methods Thirty-one children who were diagnosed as cerebral palsy and epilepsy were selected as the disease group,with 30 healthy children of the same age as the health group.Bacterial DNA was extracted from their fecal samples and the V3-V4 hypervariable regions of 16S rRNA were sequenced using the Illumina MiSeq system. Results Compared with healthy children,the children in disease group exhibited significant differences in intestinal microbial diversity.On phylum level,the relative abundance of Actinomycetes in the disease group was significantly higher(32.87% vs 3.56%),while that of Bacteroidetes was significantly lower than in the health group(22.66% vs 53.59%),respectively.On genus level,the relative abundances of Bifidobacteria and Parabacteroides in the disease group were significantly higher(26.94% vs 2.61%,5.72% vs 1.90%),and that of Enterobacteriaceae was up to 6.00%,while those of Bacteroides and Faecalibacteria were significantly lower than those in the health group(11.38% vs 45.16%,0.63% vs 12.78%),respectively.The relative abundance of Prevotella in the disease group was also lower than in the health group. Conclusion Compared with healthy children of the same age,children with cerebral palsy and epilepsy show obvious changes in intestinal flora,with obvious differences in both phylum level and genus level.
Objective To observe the changes of intestinal flora in children with cerebral palsy accompanied by epilepsy. Methods Thirty-one children who were diagnosed as cerebral palsy and epilepsy were selected as the disease group,with 30 healthy children of the same age as the health group.Bacterial DNA was extracted from their fecal samples and the V3-V4 hypervariable regions of 16S rRNA were sequenced using the Illumina MiSeq system. Results Compared with healthy children,the children in disease group exhibited significant differences in intestinal microbial diversity.On phylum level,the relative abundance of Actinomycetes in the disease group was significantly higher(32.87% vs 3.56%),while that of Bacteroidetes was significantly lower than in the health group(22.66% vs 53.59%),respectively.On genus level,the relative abundances of Bifidobacteria and Parabacteroides in the disease group were significantly higher(26.94% vs 2.61%,5.72% vs 1.90%),and that of Enterobacteriaceae was up to 6.00%,while those of Bacteroides and Faecalibacteria were significantly lower than those in the health group(11.38% vs 45.16%,0.63% vs 12.78%),respectively.The relative abundance of Prevotella in the disease group was also lower than in the health group. Conclusion Compared with healthy children of the same age,children with cerebral palsy and epilepsy show obvious changes in intestinal flora,with obvious differences in both phylum level and genus level.
引文
[1] WANG Wenjian, ZHOU Qian, DAI Wenkui, et al. Alterations of gut microbiota in infants with refractory epilepsy[J]. Chin J Microecol, 2017, 29(5): 502-505. (in Chinese) 王文建, 周潜, 戴文魁, 等. 难治性癫痫婴幼儿肠道菌群的变化[J]. 中国微生态学杂志, 2017, 29(5): 502-505.
[2] Xie G, Zhou Q, Qiu CZ, et al. Ketogenic diet poses a significant effect on imbalanced gut microbiota in infants with refractory epilepsy[J]. World J Gastroenterol, 2017, 23(33): 6164-6171.
[3] Zhang Y, Zhou S, Zhou Y, et al. Altered gut microbiome composition in children with refractory epilepsy after ketogenic diet[J]. Epilepsy Res, 2018, 145: 163-168.
[4] Olson CA, Vuong HE, Yano JM, et al. The gut microbiota mediates the anti-seizure effects of the ketogenic diet[J]. Cell, 2018, 174(2): 497.
[5] Kang DW, Park JG, Ilhan ZE, et al. Reduced incidence of Prevotella and other fermenters in intestinal microflora of autistic children[J]. PLoS One, 2013, 8(7): e68322.
[6] Scheperjans F, Aho V, Pereira PA, et al. Gut microbiota are related to Parkinson′s disease and clinical phenotype[J]. Mov Disord, 2015, 30(3): 350-358.
[7] Bercik P, Verdu EF, Foster JA, et al. Chronic gastrointestinal inflammation induces anxiety-like behavior and alters central nervous system biochemistry in mice[J]. Gastroenterology, 2010, 139(6): 2102-2112. e1.
[8] Subramanian S, Huq S, Yatsunenko T, et al. Persistent gut microbiota immaturity in malnourished Bangladeshi children[J]. Nature, 2014, 510(7505): 417-421.
[9] Smith MI, Yatsunenko T, Manary MJ, et al. Gut microbiomes of Malawian twin pairs discordant for kwashiorkor[J]. Science, 2013, 339(6119): 548-554.
[10] Monira S, Nakamura S, Gotoh K, et al. Gut microbiota of healthy and malnourished children in bangladesh[J]. Front Microbiol, 2011, 2: 228.
[11] Bercik P. The microbiota-gut-brain axis: learning from intestinal bacteria?[J]. Gut, 2011, 60(3): 288-289.
[12] Grenham S, Clarke G, Cryan JF, et al. Brain-gut-microbe communication in health and disease[J]. Front Physiol, 2011, 2: 94.
[13] Jasarevic E, Howerton CL, Howard CD, et al. Alterations in the vaginal microbiome by maternal stress are associated with metabolic reprogramming of the offspring gut and brain[J]. Endocrinology, 2015, 156(9): 3265-3276.
[14] Rapoport JL, Giedd JN, Gogtay N, et al. Neurodevelopmental model of schizophrenia: Update 2012[J]. Mol Psychiatry, 2012, 17(12): 1228-1238.
[15] Ben-Ari Y. Neuropaediatric and neuroarchaeollgy: understanding development to correct brain disorders[J]. Acta Paediatr, 2013, 102(4): 331-334.
[16] XIANG Min, QING Xiaodan, LIU Lei, et al. Progress of research on the interaction between gut microbiota and brain[J]. Chin J Microecol, 2017, 29(9): 1097-1102. (in Chinese) 向敏, 卿小丹, 刘磊, 等. 肠道菌群与大脑相互作用的研究进展[J]. 中国微生态学杂志, 2017, 29(9): 1097-1102.
[17] Chen CH, Lin CL, Kao CH. Irritable bowel syndrome increases the risk of epilepsy: A population based study[J]. Medicine (Baltimore), 2015, 94(36): e1497.
[18] 李沁芮, 韩颖, 杜军保, 等. 肠道菌群与神经精神系统疾病研究进展[J]. 生理科学进展, 2016, 47(5): 365-368.
[2] Xie G, Zhou Q, Qiu CZ, et al. Ketogenic diet poses a significant effect on imbalanced gut microbiota in infants with refractory epilepsy[J]. World J Gastroenterol, 2017, 23(33): 6164-6171.
[3] Zhang Y, Zhou S, Zhou Y, et al. Altered gut microbiome composition in children with refractory epilepsy after ketogenic diet[J]. Epilepsy Res, 2018, 145: 163-168.
[4] Olson CA, Vuong HE, Yano JM, et al. The gut microbiota mediates the anti-seizure effects of the ketogenic diet[J]. Cell, 2018, 174(2): 497.
[5] Kang DW, Park JG, Ilhan ZE, et al. Reduced incidence of Prevotella and other fermenters in intestinal microflora of autistic children[J]. PLoS One, 2013, 8(7): e68322.
[6] Scheperjans F, Aho V, Pereira PA, et al. Gut microbiota are related to Parkinson′s disease and clinical phenotype[J]. Mov Disord, 2015, 30(3): 350-358.
[7] Bercik P, Verdu EF, Foster JA, et al. Chronic gastrointestinal inflammation induces anxiety-like behavior and alters central nervous system biochemistry in mice[J]. Gastroenterology, 2010, 139(6): 2102-2112. e1.
[8] Subramanian S, Huq S, Yatsunenko T, et al. Persistent gut microbiota immaturity in malnourished Bangladeshi children[J]. Nature, 2014, 510(7505): 417-421.
[9] Smith MI, Yatsunenko T, Manary MJ, et al. Gut microbiomes of Malawian twin pairs discordant for kwashiorkor[J]. Science, 2013, 339(6119): 548-554.
[10] Monira S, Nakamura S, Gotoh K, et al. Gut microbiota of healthy and malnourished children in bangladesh[J]. Front Microbiol, 2011, 2: 228.
[11] Bercik P. The microbiota-gut-brain axis: learning from intestinal bacteria?[J]. Gut, 2011, 60(3): 288-289.
[12] Grenham S, Clarke G, Cryan JF, et al. Brain-gut-microbe communication in health and disease[J]. Front Physiol, 2011, 2: 94.
[13] Jasarevic E, Howerton CL, Howard CD, et al. Alterations in the vaginal microbiome by maternal stress are associated with metabolic reprogramming of the offspring gut and brain[J]. Endocrinology, 2015, 156(9): 3265-3276.
[14] Rapoport JL, Giedd JN, Gogtay N, et al. Neurodevelopmental model of schizophrenia: Update 2012[J]. Mol Psychiatry, 2012, 17(12): 1228-1238.
[15] Ben-Ari Y. Neuropaediatric and neuroarchaeollgy: understanding development to correct brain disorders[J]. Acta Paediatr, 2013, 102(4): 331-334.
[16] XIANG Min, QING Xiaodan, LIU Lei, et al. Progress of research on the interaction between gut microbiota and brain[J]. Chin J Microecol, 2017, 29(9): 1097-1102. (in Chinese) 向敏, 卿小丹, 刘磊, 等. 肠道菌群与大脑相互作用的研究进展[J]. 中国微生态学杂志, 2017, 29(9): 1097-1102.
[17] Chen CH, Lin CL, Kao CH. Irritable bowel syndrome increases the risk of epilepsy: A population based study[J]. Medicine (Baltimore), 2015, 94(36): e1497.
[18] 李沁芮, 韩颖, 杜军保, 等. 肠道菌群与神经精神系统疾病研究进展[J]. 生理科学进展, 2016, 47(5): 365-368.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |