不同蜜环菌属真菌胞外多糖生物功能的研究
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摘要
以11种蜜环菌为研究对象,应用深层液体发酵获得胞外多糖,采用硫酸-苯酚法确定多糖的得率,利用肿瘤细胞HepG2共培养的方法研究了胞外多糖抑制肿瘤增殖的活性,通过铁离子还原能力法(FRAP)评价蜜环菌胞外多糖的总抗氧化能力。研究结果表明,各品种间胞外多糖的得率存在着显著差异,从0. 34 g·L~(-1)到9. 31g·L~(-1)不等,体外抗肿瘤研究显示品种间差异较为显著。其中,黄盖蜜环菌胞外多糖的活性最高,其半抑制质量浓度IC50仅为253. 6 mg·L~(-1),抗氧化检测结果显示秦氏蜜环菌的胞外多糖活力最高,可以达到976. 4μmol·g~(-1)。结合发酵多糖产量发现,北方蜜环菌胞外多糖是各品种中抗肿瘤功效显著的优势品种,而芥黄蜜环菌则是抗氧化功效的优势品种。
In this study,11 species of Armillaria were taken as the research objects. The extracellular polysaccharides of 11 species were extracted from culture broth by liquid fermentation technology,and the yields of polysaccharides were determined by sulfuric acid-phenol method. The antitumor and antioxidant activities of extracellular polysaccharides were evaluated with HepG2 co-culture method and ferric reducing ability of plasma( FRAP) method,respectively. The results indicated that there were significant differences among the yields of extracellular polysaccharides with different species,from 0. 34 g·L~(-1) to 9. 31 g·L~(-1). In anti-tumor assays,the antiproliferative activities of extracellular polysaccharides from 11 species were remarkably different,and the polysaccharide of Armillaria luteopileata showed the most remarkable activities among all the species,with 253. 6 mg·L~(-1) of IC50 value. The polysaccharide of Armillaria qinii showed the highest total antioxidant capacity in antioxidant assay,up to 976. 4 μmol·g~(-1). Concerning the yields of extracellular polysaccharides,it was found that Armillar-ia borealis was advantageous species with antitumor activity,and the Armillaria sinapina was the advantageous species in antioxidant effect.
In this study,11 species of Armillaria were taken as the research objects. The extracellular polysaccharides of 11 species were extracted from culture broth by liquid fermentation technology,and the yields of polysaccharides were determined by sulfuric acid-phenol method. The antitumor and antioxidant activities of extracellular polysaccharides were evaluated with HepG2 co-culture method and ferric reducing ability of plasma( FRAP) method,respectively. The results indicated that there were significant differences among the yields of extracellular polysaccharides with different species,from 0. 34 g·L~(-1) to 9. 31 g·L~(-1). In anti-tumor assays,the antiproliferative activities of extracellular polysaccharides from 11 species were remarkably different,and the polysaccharide of Armillaria luteopileata showed the most remarkable activities among all the species,with 253. 6 mg·L~(-1) of IC50 value. The polysaccharide of Armillaria qinii showed the highest total antioxidant capacity in antioxidant assay,up to 976. 4 μmol·g~(-1). Concerning the yields of extracellular polysaccharides,it was found that Armillar-ia borealis was advantageous species with antitumor activity,and the Armillaria sinapina was the advantageous species in antioxidant effect.
引文
[1]史国生,程春雷.蜜环菌多糖的研究进展[J].中国药业,2014,23(20):124-125.
[2]袁媛,刘景圣,蔡丹.蜜环菌化学成分与药理作用的研究进展[J].农产品加工(学刊),2008,7(5):46-49.
[3]XU B K,ZHANG Y.The prevention and treatment of polysaccharide from the rhizomorph of Armillaria mellea on diabetic cataract in rat[J].J Agric Sci Technol,2014,15(7):1086-1088.
[4]沈明花,金梅花.蜜环菌多糖对小鼠急性肝损伤的保护作用[J].食品研究与开发,2014,25(3):91-93.
[5]WU J,ZHOU J,LANG Y,et al.A polysaccharide from Armillaria mellea exhibition strong in vitro anticancer activity via apoptosis-involved mechanisms[J].Int JMed Mushrooms,2012,51(4):663-667.
[6]陈亮稳,张云侠,于敏,等.蜜环菌菌索多糖延缓秀丽隐杆线虫衰老机制研究[J].中草药,2013,44(4):449-453.
[7]刘宇,龚思慧,彭楠,等.蜜环菌液态发酵培养基及补料分批发酵工艺的优化[J].食品与发酵工业,2016,42(5):187-191.
[8]周骏辉,袁媛,梁宇庭,等.蜜环菌遗传转化体系的建立[J].中药材,2017,40(8):1770-1772.
[9]刘国库,杨太新,吴和平,等.蜜环菌菌材高效培养体系的建立[J].中药材,2016,39(9):1952-1955.
[10]杨海旭.秦巴山区天麻共生蜜环菌遗传多样性研究及优良菌种的选育[D].汉中:陕西理工学院,2016.
[11]王锐,张诗悦,穆青.蜜环菌的化学成分及生物活性研究进展[J].中草药,2016,47(11):1992-1999.
[12]朱显峰,余晨晨,范书军,等.蜜环菌水溶性多糖的抗氧化活性研究[J].食品工业科技,2012,33(3):89-91.
[13]翟凤艳,张方鹏,崔明洋,等.蜜环菌提取物抗氧化及抑菌活性研究[J].河南科技学院学报(自然科学版),2013,41(3):71-74.
[14]杜刚,田孟华,高路,等.蜜环菌菌索抗氧化作用初步研究[J].云南化工,2017,44(1):14-17.
[15]GENG Y,ZHU S,CHENG P,et al.Bioassay-guided fractionation of ethyl acetate extract from Armillaria mellea attenuates inflammatory response in lipopolysaccharide(LPS)stimulated BV-2 microglia[J].Phytomedicine,2017,26:55-61.
[16]孔小卫,江力.蜜环菌胞外多糖对小鼠免疫功能的影响[J].安徽大学学报(自然科学版),2007,31(1):87-90.
[17]赵爽,刘宇,许峰,等.毛木耳多糖降血脂功效研究[J].食品科技,2013,38(6):192-195.
[18]陈健,向莹.滑子菇多糖的免疫活性及抗肿瘤作用[J].现代食品科技,2013,29(8):1800-1804.
[19]曹妍,杜木英,张帆,等.蜜环菌液体培养基及多糖提取条件的优化[J].食品科学,2011,32(S1):86-90.
[20]张丽萍,苗春艳,张秉信.红缘层孔菌多糖FP2的结构与体外抗肿瘤作用的研究[J].东北师范大学学报(自然科学版),1994,19(2):74-78.
[21]YAMASAKI K,SONS S,YAMASAKITA T,et al.Synergistic induction of lymphokine(IL-22)-activated killer activity by IL-22 and the poly saccharides lentinan and therapy of spontaneous pulmonary metastases[J].Cancer Immunol Immunother,1989,29:87-92.
[22]姜自彬,李华,曹吉超.糙皮侧耳糖蛋白的性质及体外抗肿瘤活性研究[J].中国现代应用药学,2000,17(2):127-129.
[23]陈义勇,顾小红,汤坚.桦褐孔菌多糖的抗肿瘤活性研究[J].食品与生物技术学报,2011,30(1):65-69.
[24]叶文斌,樊亮,王昱,等.黄果槲寄生果实多糖的流变学研究[J].甘肃农业大学学报,2017,52(6):141-146.
[25]张杰,朱小玲,郝鲁江,等.木糖发酵拜氏梭菌的分离及发酵特性[J].甘肃农业大学学报,2018,53(5):185-190.
[2]袁媛,刘景圣,蔡丹.蜜环菌化学成分与药理作用的研究进展[J].农产品加工(学刊),2008,7(5):46-49.
[3]XU B K,ZHANG Y.The prevention and treatment of polysaccharide from the rhizomorph of Armillaria mellea on diabetic cataract in rat[J].J Agric Sci Technol,2014,15(7):1086-1088.
[4]沈明花,金梅花.蜜环菌多糖对小鼠急性肝损伤的保护作用[J].食品研究与开发,2014,25(3):91-93.
[5]WU J,ZHOU J,LANG Y,et al.A polysaccharide from Armillaria mellea exhibition strong in vitro anticancer activity via apoptosis-involved mechanisms[J].Int JMed Mushrooms,2012,51(4):663-667.
[6]陈亮稳,张云侠,于敏,等.蜜环菌菌索多糖延缓秀丽隐杆线虫衰老机制研究[J].中草药,2013,44(4):449-453.
[7]刘宇,龚思慧,彭楠,等.蜜环菌液态发酵培养基及补料分批发酵工艺的优化[J].食品与发酵工业,2016,42(5):187-191.
[8]周骏辉,袁媛,梁宇庭,等.蜜环菌遗传转化体系的建立[J].中药材,2017,40(8):1770-1772.
[9]刘国库,杨太新,吴和平,等.蜜环菌菌材高效培养体系的建立[J].中药材,2016,39(9):1952-1955.
[10]杨海旭.秦巴山区天麻共生蜜环菌遗传多样性研究及优良菌种的选育[D].汉中:陕西理工学院,2016.
[11]王锐,张诗悦,穆青.蜜环菌的化学成分及生物活性研究进展[J].中草药,2016,47(11):1992-1999.
[12]朱显峰,余晨晨,范书军,等.蜜环菌水溶性多糖的抗氧化活性研究[J].食品工业科技,2012,33(3):89-91.
[13]翟凤艳,张方鹏,崔明洋,等.蜜环菌提取物抗氧化及抑菌活性研究[J].河南科技学院学报(自然科学版),2013,41(3):71-74.
[14]杜刚,田孟华,高路,等.蜜环菌菌索抗氧化作用初步研究[J].云南化工,2017,44(1):14-17.
[15]GENG Y,ZHU S,CHENG P,et al.Bioassay-guided fractionation of ethyl acetate extract from Armillaria mellea attenuates inflammatory response in lipopolysaccharide(LPS)stimulated BV-2 microglia[J].Phytomedicine,2017,26:55-61.
[16]孔小卫,江力.蜜环菌胞外多糖对小鼠免疫功能的影响[J].安徽大学学报(自然科学版),2007,31(1):87-90.
[17]赵爽,刘宇,许峰,等.毛木耳多糖降血脂功效研究[J].食品科技,2013,38(6):192-195.
[18]陈健,向莹.滑子菇多糖的免疫活性及抗肿瘤作用[J].现代食品科技,2013,29(8):1800-1804.
[19]曹妍,杜木英,张帆,等.蜜环菌液体培养基及多糖提取条件的优化[J].食品科学,2011,32(S1):86-90.
[20]张丽萍,苗春艳,张秉信.红缘层孔菌多糖FP2的结构与体外抗肿瘤作用的研究[J].东北师范大学学报(自然科学版),1994,19(2):74-78.
[21]YAMASAKI K,SONS S,YAMASAKITA T,et al.Synergistic induction of lymphokine(IL-22)-activated killer activity by IL-22 and the poly saccharides lentinan and therapy of spontaneous pulmonary metastases[J].Cancer Immunol Immunother,1989,29:87-92.
[22]姜自彬,李华,曹吉超.糙皮侧耳糖蛋白的性质及体外抗肿瘤活性研究[J].中国现代应用药学,2000,17(2):127-129.
[23]陈义勇,顾小红,汤坚.桦褐孔菌多糖的抗肿瘤活性研究[J].食品与生物技术学报,2011,30(1):65-69.
[24]叶文斌,樊亮,王昱,等.黄果槲寄生果实多糖的流变学研究[J].甘肃农业大学学报,2017,52(6):141-146.
[25]张杰,朱小玲,郝鲁江,等.木糖发酵拜氏梭菌的分离及发酵特性[J].甘肃农业大学学报,2018,53(5):185-190.
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