花生远缘杂交后代的氨基酸含量变异分析
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摘要
本实验室利用野生花生(A.monticola)与栽培品种(黑花生、桂花红)杂交,获得了一些具有高产性状的花生新材料。通过对这些远缘杂种后代种子的氨基酸含量进行检测,共能检测到17种水解氨基酸和20种游离氨基酸。其中含量最高的是谷氨酸,其次是天冬氨酸和精氨酸,三者的总和占总氨基酸含量的46.1%～47.3%。不同株系间氨基酸含量的变异系数有较大差异。本研究可为利用远缘杂交获得高产优质的花生新材料提供一定的理论依据。
Some new peanut materials with high yield were obtained by hybridizing wild peanut(A.monticola)with cultivated varieties(Black peanut,Guihuahong).By detecting the amino acid content of the seeds of these distant hybrids,17 hydrolysis amino acids and 20 free amino acids were found in peanut seeds.Therein the content of glutamic acid was the highest,followed by aspartic and arginineacids,the sum of the three amino acids accounted for 46.1%～47.3% of the total amino acid content.It was found that variation coefficient of amino acid content of different lines was quite different.This study can provide some theoretical basis for obtaining new peanut materials with high yield and high quality by distant hybridization.
Some new peanut materials with high yield were obtained by hybridizing wild peanut(A.monticola)with cultivated varieties(Black peanut,Guihuahong).By detecting the amino acid content of the seeds of these distant hybrids,17 hydrolysis amino acids and 20 free amino acids were found in peanut seeds.Therein the content of glutamic acid was the highest,followed by aspartic and arginineacids,the sum of the three amino acids accounted for 46.1%～47.3% of the total amino acid content.It was found that variation coefficient of amino acid content of different lines was quite different.This study can provide some theoretical basis for obtaining new peanut materials with high yield and high quality by distant hybridization.
引文
[1]栾文琪,韩守萍.山东省花生品种资源品质特性的研究[J].山东农业科学,1988(4):12-15.
[2]张吉民,于善新.花生蛋白的营养弱点及其改进途径[J].粮油食品科技,1989(3):10-11.
[3]ZHENG L,SHOCKEY J,GUO F,et al.Discovery of a new mechanism for regulation of plant triacylglycerol metabolism:The peanut diacylglycerol acyltransferase-1gene family transcriptome is highly enriched in alternative splicing variants[J].Journal of Plant Physiology,2017,219:62-70.
[4]ZHENG L,SHOCKEY J,BIAN F,et al.Variant amino acid residues alter the enzyme activity of peanut type 2diacylglycerol acyltransferases[J].Frontiers in Plant Science,2017,8:1751.
[5]阮建,单雷,李新国,等.花生FAD基因家族的全基因组鉴定与表达模式分析[J].山东农业科学,2018,50:1-9.
[6]TANG G,XU P,MA W,et al.Seed-specific expression of AtLEC1increased oil content and altered fatty acid composition in seeds of peanut(Arachis hypogaea L.)[J].Frontiers in Plant Science,2018,9:1-11.
[7]TANG G,WEI L,LIU Z,et al.Ectopic expression of peanut acyl carrier protein in tobacco alters fatty acid composition in the leaf and resistance to cold stress[J].Biologia Plantarum,2012,56:493-501.
[8]芦春斌,黄上志,福家瑞.两种不同蛋白质组成类型的花生种子甲硫氨酸含量的比较分析[J].种子,2000(1):3-5.
[9]苏秋芹.不同株型花生品质性状与主要数量性状的相关分析[J].农艺科学,2006,22:192-194.
[10]唐荣华,周汉群,蔡骥业.花生栽野种间杂交研究[J].广西农业科学,1984(1):5-8.
[11]中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准-食品中氨基酸态氮的测定[S].北京:中国标准出版社,2016.
[12]中华人民共和国农业农村部.[2019-01-12]http://zzys.agri.gov.cn/nongqing.aspx.
[13]万书波.花生品种改良与高产优质栽培[M].北京:中国农业出版社,2008.
[14]MILLER J,YOUNG CT.Protein nutritional quality of Florunner peanut meal as measured by rat[J].JAgric Food Chem,1977,25:653-657.
[15]郭陞垚,陈剑洪,谢少和,等.高蛋白红皮花生新品种泉红花1号的选育[J].福建农业学报,2016,31:1308-1311.
[16]唐兆秀,徐日荣.高蛋白花生新品种福花8号的选育[J].福建农业学报,2013,28:529-532.
[17]高华援,周玉萍,王绍伦,等.高产高蛋白早熟花生新品种吉花19的选育[J].花生学报,2016,45:66.
[18]杨顺国,郭贵敏.高蛋白花生新品种黔花生六号特征特性与高效栽培技术[J].种子,2017,36:112-113.
[19]王传堂,唐月异,王秀贞,等.远缘杂交与化学诱变相结合选育高产传统出口型大花生新品种花育9610[J].种子,2016,35:100-101.
[20]徐静,张新友,汤丰收,等.花生新品种远杂9847选育及启示[J].河南农业科学,2014,43:38-41.
[21]王传堂,唐月异,王秀贞,等.利用不亲和野生种培育抗青枯病高油酸食用型花生新品种[J].山东农业科学,2018,50:57-60.
[2]张吉民,于善新.花生蛋白的营养弱点及其改进途径[J].粮油食品科技,1989(3):10-11.
[3]ZHENG L,SHOCKEY J,GUO F,et al.Discovery of a new mechanism for regulation of plant triacylglycerol metabolism:The peanut diacylglycerol acyltransferase-1gene family transcriptome is highly enriched in alternative splicing variants[J].Journal of Plant Physiology,2017,219:62-70.
[4]ZHENG L,SHOCKEY J,BIAN F,et al.Variant amino acid residues alter the enzyme activity of peanut type 2diacylglycerol acyltransferases[J].Frontiers in Plant Science,2017,8:1751.
[5]阮建,单雷,李新国,等.花生FAD基因家族的全基因组鉴定与表达模式分析[J].山东农业科学,2018,50:1-9.
[6]TANG G,XU P,MA W,et al.Seed-specific expression of AtLEC1increased oil content and altered fatty acid composition in seeds of peanut(Arachis hypogaea L.)[J].Frontiers in Plant Science,2018,9:1-11.
[7]TANG G,WEI L,LIU Z,et al.Ectopic expression of peanut acyl carrier protein in tobacco alters fatty acid composition in the leaf and resistance to cold stress[J].Biologia Plantarum,2012,56:493-501.
[8]芦春斌,黄上志,福家瑞.两种不同蛋白质组成类型的花生种子甲硫氨酸含量的比较分析[J].种子,2000(1):3-5.
[9]苏秋芹.不同株型花生品质性状与主要数量性状的相关分析[J].农艺科学,2006,22:192-194.
[10]唐荣华,周汉群,蔡骥业.花生栽野种间杂交研究[J].广西农业科学,1984(1):5-8.
[11]中华人民共和国国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准-食品中氨基酸态氮的测定[S].北京:中国标准出版社,2016.
[12]中华人民共和国农业农村部.[2019-01-12]http://zzys.agri.gov.cn/nongqing.aspx.
[13]万书波.花生品种改良与高产优质栽培[M].北京:中国农业出版社,2008.
[14]MILLER J,YOUNG CT.Protein nutritional quality of Florunner peanut meal as measured by rat[J].JAgric Food Chem,1977,25:653-657.
[15]郭陞垚,陈剑洪,谢少和,等.高蛋白红皮花生新品种泉红花1号的选育[J].福建农业学报,2016,31:1308-1311.
[16]唐兆秀,徐日荣.高蛋白花生新品种福花8号的选育[J].福建农业学报,2013,28:529-532.
[17]高华援,周玉萍,王绍伦,等.高产高蛋白早熟花生新品种吉花19的选育[J].花生学报,2016,45:66.
[18]杨顺国,郭贵敏.高蛋白花生新品种黔花生六号特征特性与高效栽培技术[J].种子,2017,36:112-113.
[19]王传堂,唐月异,王秀贞,等.远缘杂交与化学诱变相结合选育高产传统出口型大花生新品种花育9610[J].种子,2016,35:100-101.
[20]徐静,张新友,汤丰收,等.花生新品种远杂9847选育及启示[J].河南农业科学,2014,43:38-41.
[21]王传堂,唐月异,王秀贞,等.利用不亲和野生种培育抗青枯病高油酸食用型花生新品种[J].山东农业科学,2018,50:57-60.
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