河南省花生品种(系)基于SSR分子标记的遗传多样性研究
|
摘要
花生是我国重要的经济作物和油料作物。对河南省不同类型花生品种的遗传多样性进行分析、评价具有重要意义。本研究利用200对多态性较好的SSR分子标记对河南省近年来正在推广及新选育的60个花生品种(系)进行遗传多样性研究,品种(系)间相似性分析结果表明:平均遗传相似系数为0.3,在实验分析的1770个关系组合中,仅有616个遗传相似系数大于0.5,说明这些品种大多具有较低的遗传相似性。聚类结果表明,在距离320处,60份花生品种(系)被分成了5大类群,第一类群含有32个品种,第二、第三类群分别含有8个品种,第四类含有3个品种,第五类群含有9个品种。这五大类群中,均含有来源于郑州的品种。研究结果也表明,开19-2、商花10号和开农012具有独特的遗传背景,是重要的花生资源。本研究对60个河南省花生品种(系)的遗传多样性的分析,可以为花生新品种选育提供重要的理论参考。
Peanut is an important economic oil crop in China.There is great significance to analyze and evaluate genetic diversity of different types of peanuts in Henan province.In this study,the genetic diversity of 60 peanut varieties(lines)being promoted or newly cultivated in Henan province was analyzed by using 200 pairs of polymorphic SSR markers.Genetic similarity results showed that the average genetic similarity coefficient was 0.3.Only 616 out of 1770 combinations analyzed in the experiment had genetic similarity coefficient greater than 0.5,indicating that most of these varieties had low genetic similarity.Cluster analysis showed that 60 species(lines)were divided into 5 groups,of which the first group contained 32 varieties,the second group and third group contained 8 varieties respectively,the fourth group contained 3 varieties and the fifth group contained 9 varieties.The varieties from Zhengzhou were included in all five groups.The results also showed that the Kai19-2,Shanghua10 and Kainong012 were important peanut germplasms with unique genetic background.In this study,the genetic diversity of these 60 peanut varieties in Henan province was studied,and the results could provide important theoretical references for the peanut variety breeding.
Peanut is an important economic oil crop in China.There is great significance to analyze and evaluate genetic diversity of different types of peanuts in Henan province.In this study,the genetic diversity of 60 peanut varieties(lines)being promoted or newly cultivated in Henan province was analyzed by using 200 pairs of polymorphic SSR markers.Genetic similarity results showed that the average genetic similarity coefficient was 0.3.Only 616 out of 1770 combinations analyzed in the experiment had genetic similarity coefficient greater than 0.5,indicating that most of these varieties had low genetic similarity.Cluster analysis showed that 60 species(lines)were divided into 5 groups,of which the first group contained 32 varieties,the second group and third group contained 8 varieties respectively,the fourth group contained 3 varieties and the fifth group contained 9 varieties.The varieties from Zhengzhou were included in all five groups.The results also showed that the Kai19-2,Shanghua10 and Kainong012 were important peanut germplasms with unique genetic background.In this study,the genetic diversity of these 60 peanut varieties in Henan province was studied,and the results could provide important theoretical references for the peanut variety breeding.
引文
[1]中华人民共和国农业农村部.农作物数据库[DB/OL].[2018-11-27].http://zzys.agri.gov.cn/nongqing.aspx.
[2]殷冬梅,王允,尚明照,等.花生优异种质的分子标记与遗传多样性分析[J].中国农业科学,2010,43(11):2220-2228.
[3]胡晓辉,吴兰荣,苗华荣,等.33个育成花生品种遗传多样性分析[J].花生学报,2010,39(2):24-28.
[4]夏友霖,李加纳,廖伯寿,等.川渝地区花生品种遗传多样性分析[J].中国油料作物学报,2008,30(3):300-305.
[5]张新友,韩锁义,徐静,等.花生主要品质性状的QTLs定位分析[J].中国油料作物学报,2012,34(3):311-315.
[6]HE G H,MENG R H,NEWMAN M,et al.Microsatellites as DNA markers in cultivated peanut(Arachis hypogaea L.)[J].BMC Plant Biol,2003,3(3):1-6.
[7]FERGUSON M E,BUROW M D,SCHULZE S R,et al.Microsatellite identification and characterization in peanut(Arachis hypogaea L.)[J].Theor Appl Genet,2004,108(6):1064-1070.
[8]MORETZSOHN M C,HOPKISN M S,MITCH-ELL S E,et al.Genetic diversity of peanut(Arachis hypogaea L.)and its wild relatives based on the analysis of hyper variable regions of the genome[J].BMC Plant Biol,2004,4(11):1-11.
[9]MORETZSOHN M C,LEOI L,PROITE K,et al.A microsatellite-based,gene-rich linkage map for the AA genome of Arachis(Fabaceae)[J].Theor Appl Genet,2005,111(6):1060-1071.
[10]PROITE K,LEAL-BERTIOLI S C,BERTIOLI DJ,et al.ESTs from a wild Arachis species for gene discovery and marker development[J].BMC Plant Biol,2007,7.doi:10.1186/1471-2229-7-7.
[11]TAMURA K,STECHER G,PETERSON D,et al.MEGA6:molecular evolutionary genetics analysis Version 6.0[J].Mol Biol Evol,2013,30(12):2725-2729.
[12]张新友.栽培花生产量、品质和抗病性的遗传分析与QTL定位研究[D].杭州:浙江大学,2011.
[13]姜慧芳,任小平,张晓杰,等.中国花生小核心种质SSR遗传多样性[J].中国油料作物学报,2010,32(4):472-478.
[14]姜慧芳,任小平,陈玉宁,等.中国花生地方品种与育成品种的遗传多样性[J].西北植物学报,2011,31(8):1551-1559.
[15]陈本银,姜慧芳,廖伯寿,等.野生花生种质的SSR遗传多样性[J].热带亚热带植物学报,2008,16(4):296-303.
[16]王金彦,潘丽娟,杨庆利,等.我国北方地区花生品种的遗传多样性分析[J].中国农业科技导报,2009,11(6):43-49.
[17]陈静,胡晓辉,苗华荣,等.利用SSR标记分析山东省花生区试品种的遗传多样性特点[J].中国农学通报,2008,24(12):105-110.
[18]李孟军,肖寒,卢金东,等.花生微卫星标记的研究进展[J].植物学通报,2008,25(3):373-380.
[19]康红梅,李保云,孙毅,等.花生表型及SSR遗传多样性的研究[J].植物遗传资源学报,2012,13(1):66-71.
[20]林茂,李正强,郑治洪,等.贵州省花生地方品种的遗传多样性[J].作物学报,2012,38(8):1387-1396.
[21]陈新起,汪云,李小忠,等.广东花生品种遗传多样性ISSR分析[J].南方农业学报,2011,42(7):696-699.
[22]严玫,韩锁义,董文召,等.SSR标记在中国花生研究中的应用[J].中国农学通报,2012,28(6):1-7.
[23]SARVAMANGALA C,GOWDA M V C,VARSH-NEY R K.Identification of quantitative trait loci for protein content,oil content and oil quality for groundnut(Arachis hypogaea L.)[J].Field Crops Research,2011,122:49-59.
[2]殷冬梅,王允,尚明照,等.花生优异种质的分子标记与遗传多样性分析[J].中国农业科学,2010,43(11):2220-2228.
[3]胡晓辉,吴兰荣,苗华荣,等.33个育成花生品种遗传多样性分析[J].花生学报,2010,39(2):24-28.
[4]夏友霖,李加纳,廖伯寿,等.川渝地区花生品种遗传多样性分析[J].中国油料作物学报,2008,30(3):300-305.
[5]张新友,韩锁义,徐静,等.花生主要品质性状的QTLs定位分析[J].中国油料作物学报,2012,34(3):311-315.
[6]HE G H,MENG R H,NEWMAN M,et al.Microsatellites as DNA markers in cultivated peanut(Arachis hypogaea L.)[J].BMC Plant Biol,2003,3(3):1-6.
[7]FERGUSON M E,BUROW M D,SCHULZE S R,et al.Microsatellite identification and characterization in peanut(Arachis hypogaea L.)[J].Theor Appl Genet,2004,108(6):1064-1070.
[8]MORETZSOHN M C,HOPKISN M S,MITCH-ELL S E,et al.Genetic diversity of peanut(Arachis hypogaea L.)and its wild relatives based on the analysis of hyper variable regions of the genome[J].BMC Plant Biol,2004,4(11):1-11.
[9]MORETZSOHN M C,LEOI L,PROITE K,et al.A microsatellite-based,gene-rich linkage map for the AA genome of Arachis(Fabaceae)[J].Theor Appl Genet,2005,111(6):1060-1071.
[10]PROITE K,LEAL-BERTIOLI S C,BERTIOLI DJ,et al.ESTs from a wild Arachis species for gene discovery and marker development[J].BMC Plant Biol,2007,7.doi:10.1186/1471-2229-7-7.
[11]TAMURA K,STECHER G,PETERSON D,et al.MEGA6:molecular evolutionary genetics analysis Version 6.0[J].Mol Biol Evol,2013,30(12):2725-2729.
[12]张新友.栽培花生产量、品质和抗病性的遗传分析与QTL定位研究[D].杭州:浙江大学,2011.
[13]姜慧芳,任小平,张晓杰,等.中国花生小核心种质SSR遗传多样性[J].中国油料作物学报,2010,32(4):472-478.
[14]姜慧芳,任小平,陈玉宁,等.中国花生地方品种与育成品种的遗传多样性[J].西北植物学报,2011,31(8):1551-1559.
[15]陈本银,姜慧芳,廖伯寿,等.野生花生种质的SSR遗传多样性[J].热带亚热带植物学报,2008,16(4):296-303.
[16]王金彦,潘丽娟,杨庆利,等.我国北方地区花生品种的遗传多样性分析[J].中国农业科技导报,2009,11(6):43-49.
[17]陈静,胡晓辉,苗华荣,等.利用SSR标记分析山东省花生区试品种的遗传多样性特点[J].中国农学通报,2008,24(12):105-110.
[18]李孟军,肖寒,卢金东,等.花生微卫星标记的研究进展[J].植物学通报,2008,25(3):373-380.
[19]康红梅,李保云,孙毅,等.花生表型及SSR遗传多样性的研究[J].植物遗传资源学报,2012,13(1):66-71.
[20]林茂,李正强,郑治洪,等.贵州省花生地方品种的遗传多样性[J].作物学报,2012,38(8):1387-1396.
[21]陈新起,汪云,李小忠,等.广东花生品种遗传多样性ISSR分析[J].南方农业学报,2011,42(7):696-699.
[22]严玫,韩锁义,董文召,等.SSR标记在中国花生研究中的应用[J].中国农学通报,2012,28(6):1-7.
[23]SARVAMANGALA C,GOWDA M V C,VARSH-NEY R K.Identification of quantitative trait loci for protein content,oil content and oil quality for groundnut(Arachis hypogaea L.)[J].Field Crops Research,2011,122:49-59.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |