原花青素白藜芦醇浆对大鼠酒精性脑损伤的预防性保护作用研究
|
摘要
以50只SD大鼠为实验对象,随机均分为正常对照组、模型组、原花青素白藜芦醇浆低、中、高剂量[0.5、1.5、4.5 g/(kg·bw)]干预组。除正常对照组给予等量生理盐水灌胃外,其余各组采用52°白酒灌胃建立酒精性脑损伤大鼠模型,同时灌胃给予相应剂量的原花青素白藜芦醇浆,每天1次。灌胃8 d后,通过计算体重增长幅度,检测大鼠血清超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde,MDA)含量,脑组织中SOD、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和Ca~(2+)-Mg~(2+)-三磷酸腺苷酶(adenosine triphosphate,ATPase)活性,抑制羟自由基能力,MDA、钙离子(Ca~(2+))含量,分析不同剂量的原花青素白藜芦醇浆对酒精性脑损伤大鼠的作用。结果表明:原花青素白藜芦醇浆能对抗并清除自由基,提高SOD、GSH-Px和Ca~(2+)-Mg~(2+)-ATP酶活性,提高抑制羟自由基能力,降低大鼠血清MDA和脑MDA、Ca~(2+)含量,减轻精神、行为障碍,减弱酒精对食欲和胃肠消化的不利影响,对酒精性脑损伤起到预防性保护作用。
Fifty Sprague-Dawley rats were randomly divided into normal group,model group,and low,middle,high dose group of proanthocyanidins resveratrol pulp intervention groups [0.5,1.5,4.5 g/(kg·bw)].Normal group was given normal saline in the same volume while other groups were given 52 ° liquor into stomach of rats to establish alcoholic brain damage model. Meanwhile,rats in low,middle,high proanthocyanidins resveratrol pulp groups were administered with corresponding foods once a day. After 8 days of gavage,weight gain ranges were calculated,and the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and Ca~(2+)-Mg~(2+)-ATPase,the suppression ability of hydroxyl free radical,and the contents of malondialdehyde(MDA),Ca~(2+) were detected in brain homogenate,and the activities of SOD and the contents of MDA were also detected in serum. The effects of different doses of proanthocyanidins resveratrol pulp on alcoholic brain damage in rats were analyzed. The results showed that proanthocyanidins resveratrol pulp might have preventive protection effects on alcoholic brain damage by scavenging free radicals,increasing the activity of SOD,GSH-Px,Ca~(2+)-Mg~(2+)-ATPase and the suppression ability of hydroxyl free radical,decreasing the contents of MDA amd Ca~(2+),alleviating the mental and behavioral disorders,weakening the adverse effects of alcohol on appetite and gastrointestinal digestion.
Fifty Sprague-Dawley rats were randomly divided into normal group,model group,and low,middle,high dose group of proanthocyanidins resveratrol pulp intervention groups [0.5,1.5,4.5 g/(kg·bw)].Normal group was given normal saline in the same volume while other groups were given 52 ° liquor into stomach of rats to establish alcoholic brain damage model. Meanwhile,rats in low,middle,high proanthocyanidins resveratrol pulp groups were administered with corresponding foods once a day. After 8 days of gavage,weight gain ranges were calculated,and the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and Ca~(2+)-Mg~(2+)-ATPase,the suppression ability of hydroxyl free radical,and the contents of malondialdehyde(MDA),Ca~(2+) were detected in brain homogenate,and the activities of SOD and the contents of MDA were also detected in serum. The effects of different doses of proanthocyanidins resveratrol pulp on alcoholic brain damage in rats were analyzed. The results showed that proanthocyanidins resveratrol pulp might have preventive protection effects on alcoholic brain damage by scavenging free radicals,increasing the activity of SOD,GSH-Px,Ca~(2+)-Mg~(2+)-ATPase and the suppression ability of hydroxyl free radical,decreasing the contents of MDA amd Ca~(2+),alleviating the mental and behavioral disorders,weakening the adverse effects of alcohol on appetite and gastrointestinal digestion.
引文
[1]HUSAIN K,SCOTT B R,REDDY S K,et al.Chronic ethanol and nicotine interaction on rat tissue antioxidant defense system[J].Alcohol,2001,25(2):89-97
[2]ROUACH H,HOUSE P,GENTIL M,et al.Changes in some proand antioxidants in rat cerebellum after chronic alcohol intake[J].Biochem Pharmacol,1997,53(3):539-545
[3]PIERCE D R,COOK C C,HINSON J A,et al.Are oxidative mechanisms primary on ethanol induced Purkinje neuron death of the neonatal rat?[J]Neurosci Lett,2006,40(7):130-134
[4]熊峰,周毅,赵娟.神经递质及其受体在酒精诱导神经系统损伤中的作用[J].神经损伤与功能重建,2014,9(3):243-245
[5]王佳宁,郜玉钢,臧埔,等.原花青素的提取方法与药理作用研究进展[J].上海中医药杂志,2015,49(10):94-97
[6]WANG Z,SU B,FAN S,et al.Protective effect of oligomeric proanthocyanidins against alcohol-induced liver steatosis and injury in mice[J].Biochem Biophys Research Commun,2015,458(4):757-762
[7]TANG L,YANG F,FANG Z,et al.Resveratrol ameliorates alcoholic fatty liver by inducing autophagy[J].Am J Chin Med,2016,44(6):1207-1220
[8]刘玮,钱善军,亓飞,等.白藜芦醇干预大鼠酒精性脂肪肝的研究[J].中药药理与临床,2016,32(1):72-75
[9]钟飞,高辉.野鸦椿果实水提物对急性酒精性肝损伤的保护作用[J].怀化学院学报,2012,30(2):29-31
[10]陈旭,王建国.乙醇在体内的代谢及对机体的损害[J].科技信息,2008(35):460
[11]ALBANO E.Alcohol,oxidative stress and free radical damage[J].Proc Nutr Soc,2006,65(3):278-290
[12]姜秀梅,徐小磊,殷一民,等.金针菇多糖对衰老小鼠大脑抗氧化能力的影响[J].中国老年学杂志,2016,36(23):5797-5799
[13]郭永月,陶眀煊,程光宇,等.黑牛肝菌多糖对急性酒精肝损伤小鼠的保护作用[J].中国食品学报,2016,16(1):35-41
[14]刘青青,甘仲霖,谢惠波,等.原花青素白藜芦醇浆对急性酒精性肝损伤大鼠SOD和MDA的影响[J].食品研究与开发,2017,38(19):183-186
[15]ANDREYEV A Y,TSUI H S,MILNE G L,et al.Isotope-reinforced polyunsaturated fatty acids protect mitochondria from oxidative stress[J].Free Radic Biol Med,2015,82(5):63-72
[16]唐放鸣,耿德勤,王然,等.脑缺血病人血小板肌球蛋白轻链激酶和Ca2+、Mg2+-ATP酶活性及其与[Ca2+]i关系的研究[J].中风与神经疾病杂志,1999,16(6):361-363
[2]ROUACH H,HOUSE P,GENTIL M,et al.Changes in some proand antioxidants in rat cerebellum after chronic alcohol intake[J].Biochem Pharmacol,1997,53(3):539-545
[3]PIERCE D R,COOK C C,HINSON J A,et al.Are oxidative mechanisms primary on ethanol induced Purkinje neuron death of the neonatal rat?[J]Neurosci Lett,2006,40(7):130-134
[4]熊峰,周毅,赵娟.神经递质及其受体在酒精诱导神经系统损伤中的作用[J].神经损伤与功能重建,2014,9(3):243-245
[5]王佳宁,郜玉钢,臧埔,等.原花青素的提取方法与药理作用研究进展[J].上海中医药杂志,2015,49(10):94-97
[6]WANG Z,SU B,FAN S,et al.Protective effect of oligomeric proanthocyanidins against alcohol-induced liver steatosis and injury in mice[J].Biochem Biophys Research Commun,2015,458(4):757-762
[7]TANG L,YANG F,FANG Z,et al.Resveratrol ameliorates alcoholic fatty liver by inducing autophagy[J].Am J Chin Med,2016,44(6):1207-1220
[8]刘玮,钱善军,亓飞,等.白藜芦醇干预大鼠酒精性脂肪肝的研究[J].中药药理与临床,2016,32(1):72-75
[9]钟飞,高辉.野鸦椿果实水提物对急性酒精性肝损伤的保护作用[J].怀化学院学报,2012,30(2):29-31
[10]陈旭,王建国.乙醇在体内的代谢及对机体的损害[J].科技信息,2008(35):460
[11]ALBANO E.Alcohol,oxidative stress and free radical damage[J].Proc Nutr Soc,2006,65(3):278-290
[12]姜秀梅,徐小磊,殷一民,等.金针菇多糖对衰老小鼠大脑抗氧化能力的影响[J].中国老年学杂志,2016,36(23):5797-5799
[13]郭永月,陶眀煊,程光宇,等.黑牛肝菌多糖对急性酒精肝损伤小鼠的保护作用[J].中国食品学报,2016,16(1):35-41
[14]刘青青,甘仲霖,谢惠波,等.原花青素白藜芦醇浆对急性酒精性肝损伤大鼠SOD和MDA的影响[J].食品研究与开发,2017,38(19):183-186
[15]ANDREYEV A Y,TSUI H S,MILNE G L,et al.Isotope-reinforced polyunsaturated fatty acids protect mitochondria from oxidative stress[J].Free Radic Biol Med,2015,82(5):63-72
[16]唐放鸣,耿德勤,王然,等.脑缺血病人血小板肌球蛋白轻链激酶和Ca2+、Mg2+-ATP酶活性及其与[Ca2+]i关系的研究[J].中风与神经疾病杂志,1999,16(6):361-363
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |