不同参数组合电针对炎性痛模型大鼠镇痛效应及中枢内啡肽的影响
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摘要
目的:评价不同参数组合电针对炎性痛模型大鼠的镇痛效应,筛选出电针治疗炎性痛的最佳参数组合。方法:42只雄性SD大鼠,随机分为空白组、模型组、2Hz组、100Hz组、120Hz组、2/100Hz组和2/120Hz组。用完全弗氏佐剂(CFA)建立大鼠炎性痛模型,电针干预大鼠患侧"足三里"和"昆仑"穴,于造模前,造模后24h,治疗第1、3、5、7、9、10天检测大鼠右后足机械缩足阈值(PWTs)。用放射免疫分析法(RIA)检测下丘脑β-内啡肽(β-END)与脑啡肽(ENK)含量、脊髓背角强啡肽(DYN)含量。结果:在电针干预后第7～10天,2/100Hz组和2/120Hz组大鼠PWTs不同程度高于其他电针组(P<0.01);各电针组大鼠下丘脑β-END、ENK含量均不同程度高于空白组和模型组(P<0.01,P<0.05),而2Hz电针组大鼠下丘脑ENK含量、脊髓背角DYN含量高于其他电针组(P<0.01,P<0.05)。结论:不同参数组合电针均能减轻大鼠炎性疼痛,炎性痛早期不同参数组合电针镇痛疗效相当,而后期2/100Hz或2/120Hz频率电针疗效更好。中枢内啡肽分泌是电针镇痛起效机制之一。
Objective: To evaluate the different parameters combinations electro-acupuncture(EA) on analgesia and central endorphins in inflammatory pain model rats and select the best parameter combination of electro-acupuncture treatment for inflammatory pain. Methods: A total of 42 SD male rats were randomly divided into control group(C group), model group(M group), 2 Hz EA group, 100 Hz EA group, 120 Hz EA group, 2/100 Hz EA group and 2/120 Hz EA group. The inflammatory pain model was established by injecting CFA. EA groups were given the electro-acupuncture on Zusanli(ST36) and Kunlun(BL60) of affected sideat at 24 hours before and after modeling. PWTs were detected in the 1, 3, 5, 7, 9, 10 day after the treatment. The α-END and ENK concentration of hypothalamus, as well as DYN concentration of spinal dorsal horn were measured by RIA. Results:Day7 to day10 after electro-acupuncture treatment, 2/100 Hz and 2/120 Hz EA groups PWTs was higher than other EA groups(P<0.01). The α-END and ENK concentration of hypothalamus in all EA groups were higher than C group and M group(P<0.01,P<0.05). The ENK concentration of hypothalamus and the DYN concentration of spinal dorsal horn in 2 Hz EA group was higher than the other groups(P<0.01, P<0.05). Conclusion: Different parameter combinations electro-acupuncture has analgesic action for inflammatory pain modal rat. At early stage of inflammatory pain, different parameter combinations electro-acupuncture is similar efficacious, while at late stage of inflammatory pain, 2/100 Hz or 2/120 Hz electro-acupuncture is more efficacious. The secretion of central endorphins is one of the effective mechanisms of electro-acupuncture analgesia.
Objective: To evaluate the different parameters combinations electro-acupuncture(EA) on analgesia and central endorphins in inflammatory pain model rats and select the best parameter combination of electro-acupuncture treatment for inflammatory pain. Methods: A total of 42 SD male rats were randomly divided into control group(C group), model group(M group), 2 Hz EA group, 100 Hz EA group, 120 Hz EA group, 2/100 Hz EA group and 2/120 Hz EA group. The inflammatory pain model was established by injecting CFA. EA groups were given the electro-acupuncture on Zusanli(ST36) and Kunlun(BL60) of affected sideat at 24 hours before and after modeling. PWTs were detected in the 1, 3, 5, 7, 9, 10 day after the treatment. The α-END and ENK concentration of hypothalamus, as well as DYN concentration of spinal dorsal horn were measured by RIA. Results:Day7 to day10 after electro-acupuncture treatment, 2/100 Hz and 2/120 Hz EA groups PWTs was higher than other EA groups(P<0.01). The α-END and ENK concentration of hypothalamus in all EA groups were higher than C group and M group(P<0.01,P<0.05). The ENK concentration of hypothalamus and the DYN concentration of spinal dorsal horn in 2 Hz EA group was higher than the other groups(P<0.01, P<0.05). Conclusion: Different parameter combinations electro-acupuncture has analgesic action for inflammatory pain modal rat. At early stage of inflammatory pain, different parameter combinations electro-acupuncture is similar efficacious, while at late stage of inflammatory pain, 2/100 Hz or 2/120 Hz electro-acupuncture is more efficacious. The secretion of central endorphins is one of the effective mechanisms of electro-acupuncture analgesia.
引文
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[3]杜俊英,房军帆,陈宜恬,等.电针抗大鼠骨癌痛的参数优选及其对阿片受体和前体mRNA表达的干预.中国针灸,2015,33(2):161-168
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[5]胡海宇,丁家威,吴叶琪,等.电针对痛觉敏化大鼠背根神经节蛋白酶激活受体2的影响.针刺研究,2018,43(1):14-19
[6]项璇儿,杜俊英,房军帆,等.不同病理痛早期电针镇痛频率优选及其脊髓背角TRPV1活化机制研究.中医中医药杂志,2018,33(5):1707-1712
[7]林蓓菌.电针仪设计技术及其参数的相关因素研究.广州:广州中医药大学,2010
[8]李雨衡,王文,罗层,等.电针抑制脊髓内趋化因子CX3CL1表达减轻炎性痛的实验研究.神经解剖学杂志,2016,32(2):169-173
[9]刘盈君,林小溪,方芳,等.Mas相关G蛋白偶联受体C参与电针干预慢性炎性痛及其外周δ-阿片受体机制.中华中医药杂志,2017,32(5):1988-1993
[10]周杰,庄晟坚,龚杰,等.不同频率电针对神经病理痛模型大鼠镇痛效应观察.浙江中医药大学学报,2016,40(12):887-890
[11]周杰,庄晟坚,龚杰,等.不同频率和波宽组合电刺激对健康大鼠痛阈变化的研究.浙江中医杂志,2017,52(10):764-766
[12]Malekzadeh G,Dashti-Rahmatabadi M H,Zanbagh S,et al.Mumijoattenuates chemically induced inflammatory pain in mice.AlternTher Health Med,2015,21(2):42-47
[13]梁宜,周杰,房军帆,等.一种用于清醒大鼠的柔性固定器:201420473579.9.2014-12-24
[14]华兴邦,周浩良.大鼠穴位图谱的研制.实验动物与动物实验,1991,2(1):1-5
[15]李芸,傅志俭.臭氧治疗炎性痛的应用研究.中国疼痛医学杂志,2016,22(3):168-171
[16]Old E A,Clark A K,Malcangio M.The role of glia in the spinalcord in neuropathic and inflammatory pain.Handb ExpPharmacol,2015,227:145-170
[17]Beyer A,Sch?fer M,Stein C.Antinociceptive effects of dynorphinpeptides in a model of inflammatory pain.Pain,1997,70(2-3):141-147
[18]M Lukács,K Warfvinge,J Tajti,et al.Topical dura mater applicationof CFA induces enhanced expression of c-fos and glutamate in rattrigeminal nucleus caudalis:attenuated by KYNA derivate(SZR72).Journal of Headache&Pain,2017,18(1):39-46
[19]温存,杜俊英,房军帆,等.电针干预慢性炎性痛大鼠痛情绪的前扣带皮层PKCζ调控机制.浙江中医药大学学报,2017,41(1):25-31,45
[20]池细绿,方芳,方剑乔,等.BAM22多肽对慢性炎性痛大鼠痛阈和电针镇痛作用的影响.上海针灸杂志,2014,33(5):468-471
[21]林丽莉,陈惠娥,张志灿,等.脊髓小胶质细胞激活与电针镇痛效应研究.辽宁中医杂志,2015,42(12):2300-2303
[22]陆琪赟,陈颢,崔光卫,等.电针结合吗啡对骨癌痛大鼠痛觉敏化及脊髓星形胶质细胞的活化作用.上海中医药杂志,2016,50(5):77-82
[23]庄伊洢,陈玄,叶笑然,等.宽波电脉冲电针足三里防治下肢肌萎缩研究.上海针灸杂志,2016,35(6):742-744
[24]石艳红,邵秀芹,冯珍,等.正中神经电刺激对脑外伤后昏迷促醒治疗的参数研究.中国康复理论与实践,2017,23(2):207-210
[2]王海华,王海珍,曾瑾,等.水溶性蜂胶联合阿司匹林对大鼠炎性痛作用.中成药,2015,37(6):1157-1164
[3]杜俊英,房军帆,陈宜恬,等.电针抗大鼠骨癌痛的参数优选及其对阿片受体和前体mRNA表达的干预.中国针灸,2015,33(2):161-168
[4]张慧,何胜东,胡幼平,等.电针预处理对偏头痛大鼠三叉神经尾核CB1和C-fos蛋白表达的影响.中华中医药杂志,2017,32(1):324-328
[5]胡海宇,丁家威,吴叶琪,等.电针对痛觉敏化大鼠背根神经节蛋白酶激活受体2的影响.针刺研究,2018,43(1):14-19
[6]项璇儿,杜俊英,房军帆,等.不同病理痛早期电针镇痛频率优选及其脊髓背角TRPV1活化机制研究.中医中医药杂志,2018,33(5):1707-1712
[7]林蓓菌.电针仪设计技术及其参数的相关因素研究.广州:广州中医药大学,2010
[8]李雨衡,王文,罗层,等.电针抑制脊髓内趋化因子CX3CL1表达减轻炎性痛的实验研究.神经解剖学杂志,2016,32(2):169-173
[9]刘盈君,林小溪,方芳,等.Mas相关G蛋白偶联受体C参与电针干预慢性炎性痛及其外周δ-阿片受体机制.中华中医药杂志,2017,32(5):1988-1993
[10]周杰,庄晟坚,龚杰,等.不同频率电针对神经病理痛模型大鼠镇痛效应观察.浙江中医药大学学报,2016,40(12):887-890
[11]周杰,庄晟坚,龚杰,等.不同频率和波宽组合电刺激对健康大鼠痛阈变化的研究.浙江中医杂志,2017,52(10):764-766
[12]Malekzadeh G,Dashti-Rahmatabadi M H,Zanbagh S,et al.Mumijoattenuates chemically induced inflammatory pain in mice.AlternTher Health Med,2015,21(2):42-47
[13]梁宜,周杰,房军帆,等.一种用于清醒大鼠的柔性固定器:201420473579.9.2014-12-24
[14]华兴邦,周浩良.大鼠穴位图谱的研制.实验动物与动物实验,1991,2(1):1-5
[15]李芸,傅志俭.臭氧治疗炎性痛的应用研究.中国疼痛医学杂志,2016,22(3):168-171
[16]Old E A,Clark A K,Malcangio M.The role of glia in the spinalcord in neuropathic and inflammatory pain.Handb ExpPharmacol,2015,227:145-170
[17]Beyer A,Sch?fer M,Stein C.Antinociceptive effects of dynorphinpeptides in a model of inflammatory pain.Pain,1997,70(2-3):141-147
[18]M Lukács,K Warfvinge,J Tajti,et al.Topical dura mater applicationof CFA induces enhanced expression of c-fos and glutamate in rattrigeminal nucleus caudalis:attenuated by KYNA derivate(SZR72).Journal of Headache&Pain,2017,18(1):39-46
[19]温存,杜俊英,房军帆,等.电针干预慢性炎性痛大鼠痛情绪的前扣带皮层PKCζ调控机制.浙江中医药大学学报,2017,41(1):25-31,45
[20]池细绿,方芳,方剑乔,等.BAM22多肽对慢性炎性痛大鼠痛阈和电针镇痛作用的影响.上海针灸杂志,2014,33(5):468-471
[21]林丽莉,陈惠娥,张志灿,等.脊髓小胶质细胞激活与电针镇痛效应研究.辽宁中医杂志,2015,42(12):2300-2303
[22]陆琪赟,陈颢,崔光卫,等.电针结合吗啡对骨癌痛大鼠痛觉敏化及脊髓星形胶质细胞的活化作用.上海中医药杂志,2016,50(5):77-82
[23]庄伊洢,陈玄,叶笑然,等.宽波电脉冲电针足三里防治下肢肌萎缩研究.上海针灸杂志,2016,35(6):742-744
[24]石艳红,邵秀芹,冯珍,等.正中神经电刺激对脑外伤后昏迷促醒治疗的参数研究.中国康复理论与实践,2017,23(2):207-210
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