藏族药小檗皮中6种成分的含量测定及不同品种比较研究
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摘要
为明确藏族药小檗皮中的特征成分,该文采用制备液相色谱法对小檗皮水提液中未知成分进行定向分离,利用波谱分析鉴定其结构,结果从中分离得到2个化合物,分别鉴定为蟾毒色胺内盐(Ⅰ)和阿魏酸4-O-β-D-吡喃葡萄糖苷(Ⅱ),两者均为首次从小檗皮及小檗属植物中分离得到。此外,成功建立了小檗皮中6种成分的HPLC含量测定方法,并结合化学计量学方法研究了3个品种的化学成分差异,结果药根碱和蟾毒色胺内盐为主要的种间差异成分,与甘肃小檗及鲜黄小檗相比,匙叶小檗含有明显更多的药根碱(P<0. 01),且匙叶小檗中的蟾毒色胺内盐含量明显比甘肃小檗更高(P<0. 05)。今后应进一步研究蟾毒色胺内盐的药理活性及小檗皮3个品种的药效差异,该研究结果可为小檗皮药材的质量控制、药效物质基础研究及其开发利用提供参考依据。
In order to clarify the characteristic components of Berberidis Cortex,the preparative liquid chromatography and spectral analysis methods were used to separate and identify the unknown components in the water extract of Berberidis Cortex. Two compounds were isolated and identified as bufotenidine and ferulic acid 4-O-β-D-glucopyranoside. They were both isolated for the first time from Berberidis Cortex and Berberis. In addition,an HPLC method was successfully established for simultaneously determination of six compounds in Berberidis Cortex,and chemometric methods were used to study the chemical differences among three main species of Berberidis Cortex. The results suggested that jatrorrhizine and bufotenidine are the main difference compounds among the three species.Compared with B. kansuensis and B. diaphana,B. vernae contains significantly more jatrorrhizine(P<0. 01),and the content of bufotenidine in B. vernae was significantly higher than that in B. kansuensis(P<0. 05). Considering these results,further research is necessary to reveal the pharmacological activities of bufotenidine and the pharmacodynamic differences between the three species. The results could provide a reference for quality control,the basic research on effective substances,and development of Berberidis Cortex.
In order to clarify the characteristic components of Berberidis Cortex,the preparative liquid chromatography and spectral analysis methods were used to separate and identify the unknown components in the water extract of Berberidis Cortex. Two compounds were isolated and identified as bufotenidine and ferulic acid 4-O-β-D-glucopyranoside. They were both isolated for the first time from Berberidis Cortex and Berberis. In addition,an HPLC method was successfully established for simultaneously determination of six compounds in Berberidis Cortex,and chemometric methods were used to study the chemical differences among three main species of Berberidis Cortex. The results suggested that jatrorrhizine and bufotenidine are the main difference compounds among the three species.Compared with B. kansuensis and B. diaphana,B. vernae contains significantly more jatrorrhizine(P<0. 01),and the content of bufotenidine in B. vernae was significantly higher than that in B. kansuensis(P<0. 05). Considering these results,further research is necessary to reveal the pharmacological activities of bufotenidine and the pharmacodynamic differences between the three species. The results could provide a reference for quality control,the basic research on effective substances,and development of Berberidis Cortex.
引文
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[9]李艳.藏药小檗皮DNA条形码鉴定及质量控制研究[D].成都:成都中医药大学,2016.
[10]张屏,崔征,刘雅姝,等.蟾酥中吲哚烷胺类生物碱的分离与结构鉴定[J].沈阳药科大学学报,2006,23(4):216.
[11] Chemam Y, Benayache S, Marchioni E, et al. On-line screening,isolation and identification of antioxidant compounds of Helianthemum ruficomum[J]. Molecules,2017,22(2):239.
[12] Bhattacharya S K,Sanyal A K. Neuromuscular blocking activity of bufotenidine isolated from Arundo donax L.[J]. Naturwissenschaften,1972,59(12):650.
[13] Ghosal S,Dutta S K,Sanyal A K,et al. Arundo donax phytochemical and pharmacological evaluation[J]. J Med Chem,1969,12(3):480.
[14] Deng Y C,Zhang M,Luo H Y. Identification and antimicrobial activity of two alkaloids from traditional Chinese medicinal plant Tinospora capillipes[J]. Ind Crops Prod,2012,37(1):298.
[15] Cho Y J. Anti-inflammatory effect of jatrorrhizine from Phellodendron amurense in lipopolysaccharide-stimulated raw 264. 7 cells[J]. J Appl Biol Chem,2011,54(2):114.
[16] Fu Y,Hu B R,Tang Q,et al. Hypoglycemic activity of jatrorrhizine[J]. J Huazhong Univ Sci Technol Med Sci,2005,25(5):491.
[2]西藏、青海、四川、甘肃、云南、新疆卫生局.藏药标准[S].西宁:青海人民出版社,1979:11.
[3]帝玛尔·丹增彭措.晶珠本草[M].上海:上海科学技术出版社,1986:76.
[4]周邦华,叶凡,岳丽珺,等.小檗皮水浸膏对db/db糖尿病小鼠视网膜病变的影响(Ⅱ)[J].中国实验方剂学杂志,2016,22(24):116.
[5]张燕,孟宪丽,岳丽珺,等.藏药小檗皮对糖尿病模型小鼠血糖水平影响的初步研究[J].现代生物医学进展,2013,13(19):3619.
[6]张琦,田淑琴,郑丽琴,等.藏药小檗皮中小檗碱的含量测定[J].中国民族医药杂志,2000,6(3):41.
[7]张静,吕秀梅,李艳,等.藏族药小檗皮不同药用部位中4种生物碱成分的HPLC含量测定[J].中国实验方剂学杂志,2017,23(2):37.
[8]李艳,吕秀梅,林亚丽,等.藏药小檗皮的质量标准研究[J].中国中药杂志,2016,41(4):592.
[9]李艳.藏药小檗皮DNA条形码鉴定及质量控制研究[D].成都:成都中医药大学,2016.
[10]张屏,崔征,刘雅姝,等.蟾酥中吲哚烷胺类生物碱的分离与结构鉴定[J].沈阳药科大学学报,2006,23(4):216.
[11] Chemam Y, Benayache S, Marchioni E, et al. On-line screening,isolation and identification of antioxidant compounds of Helianthemum ruficomum[J]. Molecules,2017,22(2):239.
[12] Bhattacharya S K,Sanyal A K. Neuromuscular blocking activity of bufotenidine isolated from Arundo donax L.[J]. Naturwissenschaften,1972,59(12):650.
[13] Ghosal S,Dutta S K,Sanyal A K,et al. Arundo donax phytochemical and pharmacological evaluation[J]. J Med Chem,1969,12(3):480.
[14] Deng Y C,Zhang M,Luo H Y. Identification and antimicrobial activity of two alkaloids from traditional Chinese medicinal plant Tinospora capillipes[J]. Ind Crops Prod,2012,37(1):298.
[15] Cho Y J. Anti-inflammatory effect of jatrorrhizine from Phellodendron amurense in lipopolysaccharide-stimulated raw 264. 7 cells[J]. J Appl Biol Chem,2011,54(2):114.
[16] Fu Y,Hu B R,Tang Q,et al. Hypoglycemic activity of jatrorrhizine[J]. J Huazhong Univ Sci Technol Med Sci,2005,25(5):491.