饲料中多肽类抗生素检测技术的研究进展
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摘要
本文主要综述了近年来饲料基质中多肽类抗生素检测分析方法。简要介绍了关于多肽类抗生素在饲料中使用的相关规定,以及添加使用多肽类抗生素的用途和违禁使用的危害。重点介绍了饲料中多肽类抗生素含量检测的前处理技术和检测技术,包括样品的提取、分离、纯化和检测等,并分析了不同检测技术的优缺点。最后指出了在多肽类抗生素的各种检测方法中,液相色谱-质谱联用技术用的最普遍,技术最成熟。同时,需要加大力度研究饲料中多肽类抗生素的检测,为更好地监督和规范饲料中多肽类抗生素的使用提供可靠的依据。
The methods for the detection and analysis of polypeptide antibiotics in feed in recent years were reviewed. A brief introduction on the relative laws and regulations regarding the usage of polypeptide antibiotics, as well as the advantages of using polypeptide antibiotics and the harm of illegal uses of polypeptide antibiotics in feed were summarized. Emphasis was laid on pretreatment technology and detection technology of feed matrices, including sample extraction and separation as well as purification and different detection methods, being in use for the determination of polypeptide antibiotics. Meanwhile, the advantages and disadvantages of different detection techniques in the detection of polypeptide antibiotics were analyzed. Finally in the variety of the detection technologies methods of polypeptide antibiotics, UPLC-MS/MS is the most common and the most mature detection technique used. And it is necessary to study the detection of polypeptide antibiotics in feed so as to provide reliable basis for better supervision and standardization of the use of polypeptide antibiotics in feed.
The methods for the detection and analysis of polypeptide antibiotics in feed in recent years were reviewed. A brief introduction on the relative laws and regulations regarding the usage of polypeptide antibiotics, as well as the advantages of using polypeptide antibiotics and the harm of illegal uses of polypeptide antibiotics in feed were summarized. Emphasis was laid on pretreatment technology and detection technology of feed matrices, including sample extraction and separation as well as purification and different detection methods, being in use for the determination of polypeptide antibiotics. Meanwhile, the advantages and disadvantages of different detection techniques in the detection of polypeptide antibiotics were analyzed. Finally in the variety of the detection technologies methods of polypeptide antibiotics, UPLC-MS/MS is the most common and the most mature detection technique used. And it is necessary to study the detection of polypeptide antibiotics in feed so as to provide reliable basis for better supervision and standardization of the use of polypeptide antibiotics in feed.
引文
[1]农业农村部兽医局.农业农村部办公厅关于开展兽用抗菌药使用减量化行动试点工作的通知[EB/OL].[2018-12-26].http://www.moa.gov.cn/nybgb/2018/201805/201806/t20180620_6152765.html.
[2]农业部兽医局.关于征求《关于发布<药物饲料添加剂品种目录及使用规范>的公告(征求意见稿)》意见的通知[EB/OL].[2018-12-26].www.moa.gov.cn/govpublic/SYJ/201711/t20171102_5858821.html.
[3]孙兴权,李哲,林维宣.动物源性食品中多肽类抗生素残留检测技术研究进展[J].中国食品卫生杂志,2008(3):263-266.
[4]杜鑫,苏旭,李国烈,等.超高效液相色谱-串联质谱测定猪肉中恩拉霉素残留量[J].当代畜牧,2018(27):35-36.
[5]张子臣,张翠芬,刘宪军,等.恩拉霉素的最新研究进展[J].中国抗生素杂志,2013,38(3):185-190.
[6]汪城墙,戴莉,刘凯,等.多粘类芽孢杆菌脂肽类抗生素合成与分泌机制研究进展[J].山东农业科学,2018,50(9):157-163.
[7]Y.Li,F.Wu,D.Cai,et al.MaEnhanced production of bacitracin by knocking out of amino acid permease gene yhdG in Bacillus Licheniformis DW2[J].Sheng Wu Gong Cheng Xue Bao,2018,34(6):916-927.
[8]徐加兵,魏晓东,那可,等.杆菌肽产生菌的诱变育种及培养基优化[J].中国医药工业杂志,2013,44(5):446-448+452.
[9]周欣,李宝库.杆菌肽的研究进展及应用[J].科技信息,2010(14):92-93.
[10]杨汉博,王峰.新型肽类添加剂--亚甲基水杨酸杆菌肽[J].饲料工业,2013,34(14):46-48.
[11]Stewart L L,Kim B G,Gramm B R,et al.Effect of virginiamycin on the apparent ileal digestibility of amino acids by growing pigs[J].Journal of Animal Science,2010,88(5):1718-1724.
[12]岳秀英,孙吉,吴晓岚,等.液相色谱-串联质谱法检测猪肉、鸡肉组织中维吉尼霉素M_1残留量的研究[J].中国兽药杂志,2017,51(2):40-43.
[13]于方林.颗粒包膜型杆菌肽锌对蛋鸡抗热应激效果的研究[J].辽宁畜牧兽医,2001(4):1-2.
[14]柏云江,李闯.辽宁省饲料生产企业抗生素使用情况的调研[J].现代畜牧兽医,2012(5):17-19.
[15]杜业刚,阳洪波,古丽君,等.UPLC-MS/MS法同时测定动物源性食品中8种多肽类抗生素[J].食品工业科技,2016,37(8):85-91.
[16]陈沁,房克艳,赵超敏,等.饲料中兽药含量的检测技术进展[J].现代食品科技,2018,34(10):281-290.
[17]钱卓真,罗冬莲,罗方方,等.高效液相色谱-串联质谱法测定养殖环境沉积物中多肽类抗生素残留量[J].分析化学,2016,44(6):870-875.
[18]王玉琴,刘华,郝红元,等.固相萃取-液相色谱/质谱联用法测定猪肉中3种多肽类抗生素[J].分析试验室,2017,36(1):73-77.
[19]王正彬,刘永涛,董靖,等.水产品中杆菌肽残留的微生物法检测[J].华中农业大学学报,2015,34(5):105-110.
[20]陈慧华,应永飞,杜旭奕,等.QuEChERS样品前处理-高效液相色谱法测定动物组织中那西肽残留量[J].中国兽药杂志,2014,48(2):50-53.
[21]A.R.Potts,T.Psurek,C.Jones,et al.Validation of a quantitative HPLC method for bacitracin and bacitracin zinc using EDTA as a mobile-phase modifier[J].Pharm.Biomed.Anal.,2012,70:619-623.
[22]林维宣,孙兴权,田苗,等.动物组织中粘杆菌素、杆菌肽及维吉尼霉素残留量的液相色谱-串联质谱检测[J].分析测试学报,2009,28(2):212-215.
[23]D.W.Sin,C.Ho,Y.C.Wong,et al.Analysis of major components of residual bacitracin and colistin in food samples by liquid chromatography tandem mass spectrometry[J].Anal.Chim.Acta,2005,535:23-31.
[24]雷霄云,宋云萍,李茜诺,等.毛细管电色谱-激发诱导荧光检测动物性食品中多肽类抗生素[J].色谱,2018,36(3):309-316.
[25]罗方方,钱卓真,朱世超,等.粘菌素、杆菌肽及维吉尼霉素的检测方法研究进展[J].福建水产,2012,34(6):509-513.
[26]郭桂芳,贺文庆,李维静,等.猪可食性组织中杆菌肽残留量测定的微生物学方法[J].中国兽医杂志,2014,50(2):76-78.
[27]王正彬,刘永涛,艾晓辉,等.微生物法检测水产品中粘杆菌素的残留[J].南方水产科学,2016,12(3):98-105.
[28]刘宏军,冯才伟,王瑟如,等.杆菌肽ELISA检测试剂盒的研制[J].湖南畜牧兽医,2013(5):3-6.
[29]王小莺,杨海翠.薄层色谱法检测饲料中的恩拉霉素[J].黑龙江畜牧兽医,2014(17):208-209.
[30]林仙军,朱聪英,应永飞,等.高效液相色谱法测定饲料中的那西肽[J].饲料研究,2017(12):24-27.
[31]林仙军,陆春波,包爱情,等.高效液相色谱法测定饲料中那西肽A[J].中国饲料,2018(13):54-59.
[32]D.G.Mascher,C.P.Unger,H.J.Mascher Determination of neomycin and bacitracin in human or rabbit serum by HPLC-MS/MS[J].Pharm.Biomed.Anal.,2007,43:691-700.
[33]L.F.Capitan-Vallvey,A.Titos,R.Checa,et al.High-performance liquid chromatography determination of Zn-bacitracin in animal feed by post-column derivatization and fluorescence detection[J].Chromatogr.A,2002,943:227-234.
[2]农业部兽医局.关于征求《关于发布<药物饲料添加剂品种目录及使用规范>的公告(征求意见稿)》意见的通知[EB/OL].[2018-12-26].www.moa.gov.cn/govpublic/SYJ/201711/t20171102_5858821.html.
[3]孙兴权,李哲,林维宣.动物源性食品中多肽类抗生素残留检测技术研究进展[J].中国食品卫生杂志,2008(3):263-266.
[4]杜鑫,苏旭,李国烈,等.超高效液相色谱-串联质谱测定猪肉中恩拉霉素残留量[J].当代畜牧,2018(27):35-36.
[5]张子臣,张翠芬,刘宪军,等.恩拉霉素的最新研究进展[J].中国抗生素杂志,2013,38(3):185-190.
[6]汪城墙,戴莉,刘凯,等.多粘类芽孢杆菌脂肽类抗生素合成与分泌机制研究进展[J].山东农业科学,2018,50(9):157-163.
[7]Y.Li,F.Wu,D.Cai,et al.MaEnhanced production of bacitracin by knocking out of amino acid permease gene yhdG in Bacillus Licheniformis DW2[J].Sheng Wu Gong Cheng Xue Bao,2018,34(6):916-927.
[8]徐加兵,魏晓东,那可,等.杆菌肽产生菌的诱变育种及培养基优化[J].中国医药工业杂志,2013,44(5):446-448+452.
[9]周欣,李宝库.杆菌肽的研究进展及应用[J].科技信息,2010(14):92-93.
[10]杨汉博,王峰.新型肽类添加剂--亚甲基水杨酸杆菌肽[J].饲料工业,2013,34(14):46-48.
[11]Stewart L L,Kim B G,Gramm B R,et al.Effect of virginiamycin on the apparent ileal digestibility of amino acids by growing pigs[J].Journal of Animal Science,2010,88(5):1718-1724.
[12]岳秀英,孙吉,吴晓岚,等.液相色谱-串联质谱法检测猪肉、鸡肉组织中维吉尼霉素M_1残留量的研究[J].中国兽药杂志,2017,51(2):40-43.
[13]于方林.颗粒包膜型杆菌肽锌对蛋鸡抗热应激效果的研究[J].辽宁畜牧兽医,2001(4):1-2.
[14]柏云江,李闯.辽宁省饲料生产企业抗生素使用情况的调研[J].现代畜牧兽医,2012(5):17-19.
[15]杜业刚,阳洪波,古丽君,等.UPLC-MS/MS法同时测定动物源性食品中8种多肽类抗生素[J].食品工业科技,2016,37(8):85-91.
[16]陈沁,房克艳,赵超敏,等.饲料中兽药含量的检测技术进展[J].现代食品科技,2018,34(10):281-290.
[17]钱卓真,罗冬莲,罗方方,等.高效液相色谱-串联质谱法测定养殖环境沉积物中多肽类抗生素残留量[J].分析化学,2016,44(6):870-875.
[18]王玉琴,刘华,郝红元,等.固相萃取-液相色谱/质谱联用法测定猪肉中3种多肽类抗生素[J].分析试验室,2017,36(1):73-77.
[19]王正彬,刘永涛,董靖,等.水产品中杆菌肽残留的微生物法检测[J].华中农业大学学报,2015,34(5):105-110.
[20]陈慧华,应永飞,杜旭奕,等.QuEChERS样品前处理-高效液相色谱法测定动物组织中那西肽残留量[J].中国兽药杂志,2014,48(2):50-53.
[21]A.R.Potts,T.Psurek,C.Jones,et al.Validation of a quantitative HPLC method for bacitracin and bacitracin zinc using EDTA as a mobile-phase modifier[J].Pharm.Biomed.Anal.,2012,70:619-623.
[22]林维宣,孙兴权,田苗,等.动物组织中粘杆菌素、杆菌肽及维吉尼霉素残留量的液相色谱-串联质谱检测[J].分析测试学报,2009,28(2):212-215.
[23]D.W.Sin,C.Ho,Y.C.Wong,et al.Analysis of major components of residual bacitracin and colistin in food samples by liquid chromatography tandem mass spectrometry[J].Anal.Chim.Acta,2005,535:23-31.
[24]雷霄云,宋云萍,李茜诺,等.毛细管电色谱-激发诱导荧光检测动物性食品中多肽类抗生素[J].色谱,2018,36(3):309-316.
[25]罗方方,钱卓真,朱世超,等.粘菌素、杆菌肽及维吉尼霉素的检测方法研究进展[J].福建水产,2012,34(6):509-513.
[26]郭桂芳,贺文庆,李维静,等.猪可食性组织中杆菌肽残留量测定的微生物学方法[J].中国兽医杂志,2014,50(2):76-78.
[27]王正彬,刘永涛,艾晓辉,等.微生物法检测水产品中粘杆菌素的残留[J].南方水产科学,2016,12(3):98-105.
[28]刘宏军,冯才伟,王瑟如,等.杆菌肽ELISA检测试剂盒的研制[J].湖南畜牧兽医,2013(5):3-6.
[29]王小莺,杨海翠.薄层色谱法检测饲料中的恩拉霉素[J].黑龙江畜牧兽医,2014(17):208-209.
[30]林仙军,朱聪英,应永飞,等.高效液相色谱法测定饲料中的那西肽[J].饲料研究,2017(12):24-27.
[31]林仙军,陆春波,包爱情,等.高效液相色谱法测定饲料中那西肽A[J].中国饲料,2018(13):54-59.
[32]D.G.Mascher,C.P.Unger,H.J.Mascher Determination of neomycin and bacitracin in human or rabbit serum by HPLC-MS/MS[J].Pharm.Biomed.Anal.,2007,43:691-700.
[33]L.F.Capitan-Vallvey,A.Titos,R.Checa,et al.High-performance liquid chromatography determination of Zn-bacitracin in animal feed by post-column derivatization and fluorescence detection[J].Chromatogr.A,2002,943:227-234.
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