乳腺增生动物模型的建立及其检测指标的变化分析
|
摘要
目的:通过阅读动物实验文献,对其常用动物、造模方法及检测乳腺增生动物模型的指标变化进行分析。方法:通过中国知网(CNKI)、Pubmed数据库检索与MGH相关的动物实验研究文献,共检索出97篇符合要求的期刊文献,并对其文献中相关数据进行数据分析。结果:复制MGH动物模型常用的动物是雌性未孕大鼠,常用的造模方法为肌注雌、孕激素;判断动物模型复制是否成功的指标有乳腺组织病理形态观察、乳头的直径、高度。乳头高度、直径与疾病的程度成正比;乳腺病理组织镜下观察:导管上皮细胞增生层数增加;乳腺小叶数及腺泡数明显增多。结论:复制MGH动物模型常用雌性未孕大鼠,采用雌、孕激素肌注复制MGH动物模型的方法简便、安全、成功率及成活率高,乳头高度、直径及乳腺组织病理形态观察均可作为复制动物模型判断MGH的指标。该研究的指标范围具有一定的参考价值。
Objective: To analyze the indexes changes of commonly used animals, modeling methods and animal models for detecting hyperplasia of mammary glands by reading animal experimental literature.Methods: Through the China Knowledge Network(CNKI) and Pubmed database retrieval of animal experimental research literature related to MGH, a total of 97 journal articles that met the requirements were retrieved and the data in the literature was analyzed. Results: Animals commonly used in the animal model of MGH replication were female non-pregnant rats. The commonly used modeling methods were intramuscular injection of estrogen and progesterone. The indicators for judging the successful replication of the animal model included pathological morphology of breast tissue and diameter and height of the nipple. Nipple height, diameter and the degree of disease werepositive proportional. The breast pathological tissue microscopic observation showed increased number of ductal epithelial cell proliferation; breast lobular number and acini increased significantly.Conclusions: The replication of MGH animal model is commonly used in female non-pregnant rats and the animal model of intramuscular injection of female and progesterone to MGH animal model is simple and safe with high success rate and survival rate. The nipple height, diameter and pathological morphology of breast tissuecan be used as indicators of MGH in replicating animal models. The index range of the study has a certain reference value.
Objective: To analyze the indexes changes of commonly used animals, modeling methods and animal models for detecting hyperplasia of mammary glands by reading animal experimental literature.Methods: Through the China Knowledge Network(CNKI) and Pubmed database retrieval of animal experimental research literature related to MGH, a total of 97 journal articles that met the requirements were retrieved and the data in the literature was analyzed. Results: Animals commonly used in the animal model of MGH replication were female non-pregnant rats. The commonly used modeling methods were intramuscular injection of estrogen and progesterone. The indicators for judging the successful replication of the animal model included pathological morphology of breast tissue and diameter and height of the nipple. Nipple height, diameter and the degree of disease werepositive proportional. The breast pathological tissue microscopic observation showed increased number of ductal epithelial cell proliferation; breast lobular number and acini increased significantly.Conclusions: The replication of MGH animal model is commonly used in female non-pregnant rats and the animal model of intramuscular injection of female and progesterone to MGH animal model is simple and safe with high success rate and survival rate. The nipple height, diameter and pathological morphology of breast tissuecan be used as indicators of MGH in replicating animal models. The index range of the study has a certain reference value.
引文
[1] Huang Jihan,Tang Haitao,Cao Sumin, et al. Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology[J] .Evid Based Complement Alternat Med, 2017, 2017: 1930598.
[2] Lv Pin,Chong Yuping,Zou Huagang, et al. A Study of Using Massage Therapy Accompanied with Stretching Exercise for Rehabilitation of Mammary Gland Hyperplasia[J] .Biomed Res Int, 2016, 2016: 9426167.
[3] Fan Yingyi,Pei Xiaohua,Liu Zhaolan, et al. Effectiveness of external Sanjierupi Gao on mastalgia caused by mammary gland hyperplasia: a placebo controlled trial[J] .J Tradit Chin Med, 2013, 33(5): 603-607.
[4] Chen Xi,Zhang Jian-Guo,Liu Yang. Experimental study on Ruxian Pill I in treating mammary gland hyperplasia rabbits[J] .Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007, 27(9): 822-826.
[5] Xiu He-ming,Liu Ii-jun,Liu Yan-qing. Observation on the effect of acupuncture of "Wuyi" (ST 15) and multiple acupoints on the size of lacteal gland in rats with cyclomastopathy[J] .Zhen Ci Yan Jiu, 2007, 32(3): 179-181.
[6] Liu Li-Jun,Du Hui-Lan,Jin Ya-Ci, et al. Effects of combined acupuncture and medicine therapy on the breast tissue and expressions of estrogen receptor subgroups in the rat of hyperplasia of mammary glands[J] .Zhongguo Zhen Jiu, 2007, 27(4): 279-283.
[7] 王建久,徐鸿婕,江泓颖,等.乳复康治疗乳腺增生病的作用机制[J].中国医院药学杂志,2010,30(20):1743-1744.
[8] 姜云云,叶光明,沐韦,等.乳癖消减方抗实验性大鼠乳腺增生作用[J].中国实验方剂学杂志,2013,19(12):269-272.
[9] 马民,张桂娟,马义,等.活血散结中药外用治疗老年乳腺增生病模型大鼠的疗效研究[J].中国老年学杂志,2009,29(21):2739-2742.
[10] 周艳玲,王丽娜,肖洪彬,等.乳癖消颗粒治疗大鼠乳腺增生的实验研究[J].中华中医药杂志,2009,24(11):1512-1513.
[11] 余成浩,李文军,王帅,等.复方美登木片防治大鼠乳腺增生的研究[J].中药药理与临床,2012,28(2):138-140.
[12] 刘红春,刘旺根,杨丽萍.桂枝茯苓胶囊对乳腺增生病大鼠内分泌和免疫功能的影响[J].中国临床康复,2005(2):194-195.
[13] 段好刚,魏玉辉,李波霞,等.疏乳消块丸对大鼠乳腺增生的治疗作用及其机制[J].中国医院药学杂志,2012,32(16):1273-1276.
[14] 邢松丽,罗小光,刘凯.刮痧及中药对乳腺增生病大鼠血清性激素研究的影响[J].中华中医药杂志,2012,27(7):1950-1952.
[15] 王雄,吴金虎,陈永刚,等.回乳抑增Ⅰ号对实验性乳腺增生大鼠的作用及其机制研究[J].中国实验方剂学杂志,2013,19(13):274-279.
[16] 张琳,张彬,马丽杰.蒙药乳腺-Ⅰ号对实验性乳腺增生大鼠血脂及血清性激素水平的影响[J].时珍国医国药,2012,23(4):785-787.
[17] 徐庆鑫,谷丽艳,解静茹,等.针刺对乳腺增生大鼠乳腺组织Fasl、Fas表达的影响[J].中华中医药学刊,2017,35(11):2895-2898.
[18] 李祥,冯程,郭涛,等.肋间神经切断对大鼠乳腺增生的影响[J].重庆医科大学学报,2013,38(11):1335-1340.
[19] 邓国兴,王文智,李春香,等.乳康平对“肝郁型”乳腺增生病模型的干预作用[J].中国中医基础医学杂志,2010,16(9):777-779
[20] 常秀娟,周军,张帅,等.桂枝茯苓胶囊对乳腺增生大鼠性激素水平和乳腺组织的影响[J].中国中药杂志,2014,39(21):4139-4142.
[21] Li Xiaoliang,Xin Ping,Wang Changfu, et al. Mechanisms of Traditional Chinese Medicine in the Treatment of Mammary Gland Hyperplasia[J].Am J Chin Med, 2017, 45(3): 443-458.
[22] Chen Dong,Xia Youbing,Ling Lijun, et al. Effect observation of float needle combined with reperfusion activity for pain induced by cyclomastopathy[J] .Zhongguo Zhen Jiu, 2016, 36(3): 251-255.
[23] Ma Min,Zhai He-ning. Thinking path and method for establishing the disease/Chinese medicine syndrome conjugated pattern of a mammary hyperplasia animal model[J].Chin J Integr Med, 2010, 16(5): 457-463.
[24] Chang Xiu-juan,Zhou Jun,Zhang Shuai, et al. Effects of guizhi fuling capsule on sex hormone levels and breast issue morphology of mammary gland hyperplasia model rats[J].Zhongguo Zhong Yao Za Zhi, 2014, 39(21): 4139-4142.
[25] Zhou Lei,Lu Xia,Feng Zhen-gen, et al. Effect of acupoint-catgut-embedment on mammary gland hyperplasia[J] .Zhen Ci Yan Jiu, 2011, 36(1): 52-56.
[26] Zhang Jian,Sun Yibin,Liu Yunhai, et al. Synergistic effects of androgen and estrogen on the mouse uterus and mammary gland[J].Oncol Rep, 2004, 12(4): 709-716.
[27] Milliken Erin L,Ameduri Rebecca K,Landis Melissa D, et al. Ovarian hyperstimulation by LH leads to mammary gland hyperplasia and cancer predisposition in transgenic mice[J].Endocrinology, 2002, 143(9): 3671-3680.
[28] Thakkar JP,Mehta DG.A review of an unfavorable subset of breast cancer:estrogen receptor positive progesterone recaptor negative[J].Oncologist,2011,16(3):276-285.
[29] Zheng Juanjuan,Zhao Yi,Wang Yafang, et al. The infrared radiation temperature characteristic of acupoints of mammary gland hyperplasia patients[J].Evid Based Complement Alternat Med, 2013, 2013: 567987.
[30] 向骏马,张蓉,邢茂,等.中药治疗乳腺增生动物模型评价指标的研究进展[J].中国药房,2015,26(4):550-552.
[31] QIAN Li-qi,PEI Xiao-hua,CHEN Rong-ming, et al.Effect of Lirukang Oral Liquid on Neurotransmitters and Estrogen in Rats with Hyperplasia of Mammary Gland[J].Chinese Journal of Integrated Traditional and Western Medicine,2004(2):131-134.
[32] 陈枫,袁婉丽,张岱权,等.分时针刺胆经穴位对卵巢缺失大鼠 TNF-β、IL-4以及T淋巴细胞亚群的影响[J].中国免疫学杂志,2011,27(6): 518-520.
[33] 张拴成,肖红玲,王丽娜, 等.消瘀通络散对乳腺增生大鼠乳头高度、直径、E2、ER蛋白表达水平及病理组织形态的影响[J].河北中医药学报,2012(3):5-7.
[2] Lv Pin,Chong Yuping,Zou Huagang, et al. A Study of Using Massage Therapy Accompanied with Stretching Exercise for Rehabilitation of Mammary Gland Hyperplasia[J] .Biomed Res Int, 2016, 2016: 9426167.
[3] Fan Yingyi,Pei Xiaohua,Liu Zhaolan, et al. Effectiveness of external Sanjierupi Gao on mastalgia caused by mammary gland hyperplasia: a placebo controlled trial[J] .J Tradit Chin Med, 2013, 33(5): 603-607.
[4] Chen Xi,Zhang Jian-Guo,Liu Yang. Experimental study on Ruxian Pill I in treating mammary gland hyperplasia rabbits[J] .Zhongguo Zhong Xi Yi Jie He Za Zhi, 2007, 27(9): 822-826.
[5] Xiu He-ming,Liu Ii-jun,Liu Yan-qing. Observation on the effect of acupuncture of "Wuyi" (ST 15) and multiple acupoints on the size of lacteal gland in rats with cyclomastopathy[J] .Zhen Ci Yan Jiu, 2007, 32(3): 179-181.
[6] Liu Li-Jun,Du Hui-Lan,Jin Ya-Ci, et al. Effects of combined acupuncture and medicine therapy on the breast tissue and expressions of estrogen receptor subgroups in the rat of hyperplasia of mammary glands[J] .Zhongguo Zhen Jiu, 2007, 27(4): 279-283.
[7] 王建久,徐鸿婕,江泓颖,等.乳复康治疗乳腺增生病的作用机制[J].中国医院药学杂志,2010,30(20):1743-1744.
[8] 姜云云,叶光明,沐韦,等.乳癖消减方抗实验性大鼠乳腺增生作用[J].中国实验方剂学杂志,2013,19(12):269-272.
[9] 马民,张桂娟,马义,等.活血散结中药外用治疗老年乳腺增生病模型大鼠的疗效研究[J].中国老年学杂志,2009,29(21):2739-2742.
[10] 周艳玲,王丽娜,肖洪彬,等.乳癖消颗粒治疗大鼠乳腺增生的实验研究[J].中华中医药杂志,2009,24(11):1512-1513.
[11] 余成浩,李文军,王帅,等.复方美登木片防治大鼠乳腺增生的研究[J].中药药理与临床,2012,28(2):138-140.
[12] 刘红春,刘旺根,杨丽萍.桂枝茯苓胶囊对乳腺增生病大鼠内分泌和免疫功能的影响[J].中国临床康复,2005(2):194-195.
[13] 段好刚,魏玉辉,李波霞,等.疏乳消块丸对大鼠乳腺增生的治疗作用及其机制[J].中国医院药学杂志,2012,32(16):1273-1276.
[14] 邢松丽,罗小光,刘凯.刮痧及中药对乳腺增生病大鼠血清性激素研究的影响[J].中华中医药杂志,2012,27(7):1950-1952.
[15] 王雄,吴金虎,陈永刚,等.回乳抑增Ⅰ号对实验性乳腺增生大鼠的作用及其机制研究[J].中国实验方剂学杂志,2013,19(13):274-279.
[16] 张琳,张彬,马丽杰.蒙药乳腺-Ⅰ号对实验性乳腺增生大鼠血脂及血清性激素水平的影响[J].时珍国医国药,2012,23(4):785-787.
[17] 徐庆鑫,谷丽艳,解静茹,等.针刺对乳腺增生大鼠乳腺组织Fasl、Fas表达的影响[J].中华中医药学刊,2017,35(11):2895-2898.
[18] 李祥,冯程,郭涛,等.肋间神经切断对大鼠乳腺增生的影响[J].重庆医科大学学报,2013,38(11):1335-1340.
[19] 邓国兴,王文智,李春香,等.乳康平对“肝郁型”乳腺增生病模型的干预作用[J].中国中医基础医学杂志,2010,16(9):777-779
[20] 常秀娟,周军,张帅,等.桂枝茯苓胶囊对乳腺增生大鼠性激素水平和乳腺组织的影响[J].中国中药杂志,2014,39(21):4139-4142.
[21] Li Xiaoliang,Xin Ping,Wang Changfu, et al. Mechanisms of Traditional Chinese Medicine in the Treatment of Mammary Gland Hyperplasia[J].Am J Chin Med, 2017, 45(3): 443-458.
[22] Chen Dong,Xia Youbing,Ling Lijun, et al. Effect observation of float needle combined with reperfusion activity for pain induced by cyclomastopathy[J] .Zhongguo Zhen Jiu, 2016, 36(3): 251-255.
[23] Ma Min,Zhai He-ning. Thinking path and method for establishing the disease/Chinese medicine syndrome conjugated pattern of a mammary hyperplasia animal model[J].Chin J Integr Med, 2010, 16(5): 457-463.
[24] Chang Xiu-juan,Zhou Jun,Zhang Shuai, et al. Effects of guizhi fuling capsule on sex hormone levels and breast issue morphology of mammary gland hyperplasia model rats[J].Zhongguo Zhong Yao Za Zhi, 2014, 39(21): 4139-4142.
[25] Zhou Lei,Lu Xia,Feng Zhen-gen, et al. Effect of acupoint-catgut-embedment on mammary gland hyperplasia[J] .Zhen Ci Yan Jiu, 2011, 36(1): 52-56.
[26] Zhang Jian,Sun Yibin,Liu Yunhai, et al. Synergistic effects of androgen and estrogen on the mouse uterus and mammary gland[J].Oncol Rep, 2004, 12(4): 709-716.
[27] Milliken Erin L,Ameduri Rebecca K,Landis Melissa D, et al. Ovarian hyperstimulation by LH leads to mammary gland hyperplasia and cancer predisposition in transgenic mice[J].Endocrinology, 2002, 143(9): 3671-3680.
[28] Thakkar JP,Mehta DG.A review of an unfavorable subset of breast cancer:estrogen receptor positive progesterone recaptor negative[J].Oncologist,2011,16(3):276-285.
[29] Zheng Juanjuan,Zhao Yi,Wang Yafang, et al. The infrared radiation temperature characteristic of acupoints of mammary gland hyperplasia patients[J].Evid Based Complement Alternat Med, 2013, 2013: 567987.
[30] 向骏马,张蓉,邢茂,等.中药治疗乳腺增生动物模型评价指标的研究进展[J].中国药房,2015,26(4):550-552.
[31] QIAN Li-qi,PEI Xiao-hua,CHEN Rong-ming, et al.Effect of Lirukang Oral Liquid on Neurotransmitters and Estrogen in Rats with Hyperplasia of Mammary Gland[J].Chinese Journal of Integrated Traditional and Western Medicine,2004(2):131-134.
[32] 陈枫,袁婉丽,张岱权,等.分时针刺胆经穴位对卵巢缺失大鼠 TNF-β、IL-4以及T淋巴细胞亚群的影响[J].中国免疫学杂志,2011,27(6): 518-520.
[33] 张拴成,肖红玲,王丽娜, 等.消瘀通络散对乳腺增生大鼠乳头高度、直径、E2、ER蛋白表达水平及病理组织形态的影响[J].河北中医药学报,2012(3):5-7.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |