杨梅酒对小鼠腹泻的防治作用及其作用机理
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摘要
为研究杨梅酒对腹泻的防治效果及其作用机制,采用生长曲线法研究了杨梅酒对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌生长增殖的影响,分析乙酸乙酯、乙醚等溶剂萃取的杨梅酒活性组分的体外抑菌效果,并以腹泻模型小鼠为对象,探究了不同杨梅酒萃取物对小鼠腹泻的防治效果。结果表明,与生理盐水和高粱烧相比,杨梅酒对致病菌生长增殖的抑制作用十分明显且杨梅酒乙酸乙酯提取物的抑菌作用最强。杨梅酒乙酸乙酯提取物对模型小鼠腹泻的防治效果最佳,能够显著抑制NF-κB信号通路的激活,抑制IL-8、TNF-α等炎症因子的表达,说明杨梅酒中活性成分可通过抑制NF-κB信号通路,发挥抗炎作用,这为阐释杨梅酒防治腹泻的分子机制提供了理论参考,为进一步探明杨梅酒中具有抗菌消炎作用的活性成分及其药理作用提供了新线索。
To study the control effects of Chinese bayberry wine on the diarrhea and investigate its mechanism,growth curve method was used to determine the effects on E. coli,S. aureus,and B. subtilis. The antibacterial activities of functional ingredients extracted by ethyl acetate,ether or water were analyzed by in vitro experiments. The anti-diarrheal function of the active ingredients was investigated in a mice model. The results showed Chinese bayberry wine significantly inhibited the growth and proliferation of the pathogenic bacteria compared to normal saline or kaoliang spirit,the ethyl acetate extract showed stronger antibacterial and anti-diarrheal action,which could obviously suppress the activation of NF-κB signaling pathway and downregulate the expression of IL-8 and TNF-α. In conclusion,Chinese bayberry wine played important roles on anti-inflammation by inhibiting the activation of NF-κB signaling pathway. The results of this study will provide important references for disclosing the anti-diarrheal molecular mechanism of Chinese bayberry wine,and supply new clues for exploring the anti-inflammatory active ingredient and its pharmacological action.
To study the control effects of Chinese bayberry wine on the diarrhea and investigate its mechanism,growth curve method was used to determine the effects on E. coli,S. aureus,and B. subtilis. The antibacterial activities of functional ingredients extracted by ethyl acetate,ether or water were analyzed by in vitro experiments. The anti-diarrheal function of the active ingredients was investigated in a mice model. The results showed Chinese bayberry wine significantly inhibited the growth and proliferation of the pathogenic bacteria compared to normal saline or kaoliang spirit,the ethyl acetate extract showed stronger antibacterial and anti-diarrheal action,which could obviously suppress the activation of NF-κB signaling pathway and downregulate the expression of IL-8 and TNF-α. In conclusion,Chinese bayberry wine played important roles on anti-inflammation by inhibiting the activation of NF-κB signaling pathway. The results of this study will provide important references for disclosing the anti-diarrheal molecular mechanism of Chinese bayberry wine,and supply new clues for exploring the anti-inflammatory active ingredient and its pharmacological action.
引文
[1]胡桂仙,邵圣枝,张永志,朱加虹,赵首萍,袁玉伟.杨梅中稳定同位素和多元素特征在其产地溯源中的应用[J].核农学报,2017,31(12):2450-2459
[2]林旭东,凌建刚,朱麟,康孟利.杨梅活性成分的药理作用与产品开发[J].农产品加工,2015(10):56-59,62
[3] Li J,Han Q,Chen W,Ye L. Antimicrobial activity of Chinesebayberry extract for the preservation of surimi[J]. Journal of theScience of Food and Agriculture,2012,92(11):2358-2365
[4] Kim H H,Oh M H,Park K J,Heo J H,Lee M W. Anti-inflammatory activity of sulfate-containing phenolic compoundsisolated from the leaves of Myrica rubra[J]. Fitoterapia,2014,92:188-193
[5] Fu Y,Qiao L,Cao Y,Zhou X,Liu Y,Ye X. Structural elucidationand antioxidant activities of proanthocyanidins from Chinese bayberry(Myrica rubra Sieb. et Zucc.)leaves[J]. PLoS One,2014,9(5):e96162
[6] Zhang Y,Chen S,Wei C,Rankin G O,Ye X,Chen Y C. Dietarycompound proanthocyanidins from Chinese bayberry(Myrica rubraSieb. et Zucc.)leaves attenuate chemotherapy-resistant ovariancancer stem cell traits via targeting the Wnt/β-catenin signalingpathway and inducing G1 cell cycle arrest[J]. Food&Function,2018,9(1):525-533
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[8] Chen W,Zhou S,Zheng X. A new function of Chinese bayberryextract:Protection against oxidative DNA damage[J]. LWT-FoodScience and Technology,2015,60(2):1200-1205
[9] Zhou X, Chen S, Ye X. The anti-obesity properties of theproanthocyanidin extract from the leaves of Chinese bayberry(Myricarubra Sieb.et Zucc.)[J]. Food&Function,2017,8(9):3259-3270
[10]刘合生,柳余莉,陈秋平,曹少谦,戚向阳.杨梅黄酮类化合物对α-葡萄糖苷酶活性的抑制作用[J].核农学报,2018,32(1):70-77
[11] Yu L,Cai W,Zhang Y,Feng L,Huang C. Red bayberry extractprevents high-fat diet-induced metabolic disorders in C57BL/6 mice[J]. Journal of Functional Foods,2015,14:278-288
[12] Zhang X,Huang H,Zhao X,Lv Q,SunC,Li X,Chen K. Effectsof flavonoids-rich Chinese bayberry(Myrica rubra Sieb. et Zucc.)pulp extracts on glucose consumption in human HepG2 cells[J].Journal of Functional Foods,2015,14:144-153
[13] Sun C D,Zhang B,Zhang J K,Xu C J,Wu Y L,Li X,Chen K S.Cyanidin-3-glucoside-rich extract from Chinese bayberry fruitprotects pancreaticβcells and ameliorates hyperglycemia instreptozotocin-induced diabetic mice[J]. Journal of Medicinal Food,2012,15(3):288-298
[14] Yao W R,Wang H Y,Wang S T,Sun S L,Zhou J,Luan Y Y.Assessment of the antibacterial activity and the antidiarrheal functionof flavonoids from bayberry fruit[J]. Journal of Agricultural and FoodChemistry,2011,59(10):5312-5317
[15]周洁,汪仕韬,孙石磊,汪何雅,姚卫蓉.杨梅果实提取物抗小鼠腹泻作用的研究[J].中国微生态学杂志,2009,21(4):323-326
[16]周洁.杨梅果实提取物的体内抑菌性研究及其主要活性成分的分离鉴定[D].无锡:江南大学,2009:13-16
[17]丁慧,胡龙华,钟桥石,杭亚平,刘衍伶,俞凤,陈艳慧,胡晓彦,张黎明,章白苓,张楠,王小中. 2015年临床常见分离菌分布及耐药特性分析[J].中国抗生素杂志,2017,42(11):967-973
[18]商军,顾欣,张文刚,姜芹,孙冰清.猪、鸡源分离粪肠球菌的核糖体分型及耐药性分析[J].中国抗生素杂志,2017,42(3):230-236
[19]聂荣,刘素纯,王建新.浸泡型杨梅酒花色苷稳定性的研究[J].酿酒科技,2014(5):56-58
[20]秦红,宋庆庆,华晓燕.杨梅果酒对肠道细菌的抑菌效果[J].贵州农业科学,2012,40(10):157-159
[21]徐岩,范文来,葛向阳,黄永光.科学认识中国白酒中的生物活性成分[J].酿酒科技,2013(9):1-6
[22] Qian J,Zhao W,Miao X,Li L,Zhang D. Sam68 modulatesapoptosis of intestinal epithelial cells via mediating NF-κB activationin ulcerative colitis[J]. Molecular Immunology,2016,75:48-59
[23] Dou W,Zhang J,Ren G,Ding L,Sun A,Deng C,Wu X,Wei X,Mani S,Wang Z. Mangiferin attenuates the symptoms of dextransulfate sodium-induced colitis in mice via NF-κB and MAPKsignaling inactivation[J]. International Immunopharmacology,2014,23(1):170-178
[24] Daniele S,Da Pozzo E,Zappelli E,Martini C. Trazodone treatmentprotects neuronal-like cells from inflammatory insult by inhibitingNF-κB,p38 and JNK[J]. Cellular Signalling,2015,27(8):1609-1629
[25] Villalba M,Fredericksen F,Otth C,OlavarríaⅤH. Molecularcharacterization of the bovine IER3 gene:Down-regulation of IL-8by blocking NF-κB activity mediated by IER3 overexpression inMDBK cells infected with bovine viral diarrhea virus-1[J].Molecular Immunology,2017,92:169-179
[26] Uwada J,Yazawa T,Islam M T,Khan M,Krug S,Fromm M,Karaki SⅠ, Suzuki Y, Kuwahara A, Yoshiki H, Sada K,MuramatsuⅠ, Taniguchi T. Activation of muscarinic receptorsprevents TNF-α-mediated intestinal epithelial barrier disruptionthrough p38 MAPK[J]. Cellular Signalling,2017,35:188-196
[27] Jeong C H, Seok J S, Petriello M C, Han S G. Arsenicdownregulates tight junction claudin proteins through p38 andNF-κB in intestinal epithelial cell line,HT-29[J]. Toxicology,2017,379:31-39
[28] Guo H,Zhong R,Liu Y,Jiang X,Tang X,Li Z,Xia M,Ling W.Effects of bayberry juice on inflammatory and apoptotic markers inyoung adults with features of non-alcoholic fatty liver disease[J].Nutrition,2014,30(2):198-203
[2]林旭东,凌建刚,朱麟,康孟利.杨梅活性成分的药理作用与产品开发[J].农产品加工,2015(10):56-59,62
[3] Li J,Han Q,Chen W,Ye L. Antimicrobial activity of Chinesebayberry extract for the preservation of surimi[J]. Journal of theScience of Food and Agriculture,2012,92(11):2358-2365
[4] Kim H H,Oh M H,Park K J,Heo J H,Lee M W. Anti-inflammatory activity of sulfate-containing phenolic compoundsisolated from the leaves of Myrica rubra[J]. Fitoterapia,2014,92:188-193
[5] Fu Y,Qiao L,Cao Y,Zhou X,Liu Y,Ye X. Structural elucidationand antioxidant activities of proanthocyanidins from Chinese bayberry(Myrica rubra Sieb. et Zucc.)leaves[J]. PLoS One,2014,9(5):e96162
[6] Zhang Y,Chen S,Wei C,Rankin G O,Ye X,Chen Y C. Dietarycompound proanthocyanidins from Chinese bayberry(Myrica rubraSieb. et Zucc.)leaves attenuate chemotherapy-resistant ovariancancer stem cell traits via targeting the Wnt/β-catenin signalingpathway and inducing G1 cell cycle arrest[J]. Food&Function,2018,9(1):525-533
[7] Sun C,Huang H,Xu C,Li X,Chen K. Biological activities ofextracts from Chinese bayberry(Myrica rubra Sieb. et Zucc.):areview[J]. Plant Foods for Human Nutrition,2013,68(2):97-106
[8] Chen W,Zhou S,Zheng X. A new function of Chinese bayberryextract:Protection against oxidative DNA damage[J]. LWT-FoodScience and Technology,2015,60(2):1200-1205
[9] Zhou X, Chen S, Ye X. The anti-obesity properties of theproanthocyanidin extract from the leaves of Chinese bayberry(Myricarubra Sieb.et Zucc.)[J]. Food&Function,2017,8(9):3259-3270
[10]刘合生,柳余莉,陈秋平,曹少谦,戚向阳.杨梅黄酮类化合物对α-葡萄糖苷酶活性的抑制作用[J].核农学报,2018,32(1):70-77
[11] Yu L,Cai W,Zhang Y,Feng L,Huang C. Red bayberry extractprevents high-fat diet-induced metabolic disorders in C57BL/6 mice[J]. Journal of Functional Foods,2015,14:278-288
[12] Zhang X,Huang H,Zhao X,Lv Q,SunC,Li X,Chen K. Effectsof flavonoids-rich Chinese bayberry(Myrica rubra Sieb. et Zucc.)pulp extracts on glucose consumption in human HepG2 cells[J].Journal of Functional Foods,2015,14:144-153
[13] Sun C D,Zhang B,Zhang J K,Xu C J,Wu Y L,Li X,Chen K S.Cyanidin-3-glucoside-rich extract from Chinese bayberry fruitprotects pancreaticβcells and ameliorates hyperglycemia instreptozotocin-induced diabetic mice[J]. Journal of Medicinal Food,2012,15(3):288-298
[14] Yao W R,Wang H Y,Wang S T,Sun S L,Zhou J,Luan Y Y.Assessment of the antibacterial activity and the antidiarrheal functionof flavonoids from bayberry fruit[J]. Journal of Agricultural and FoodChemistry,2011,59(10):5312-5317
[15]周洁,汪仕韬,孙石磊,汪何雅,姚卫蓉.杨梅果实提取物抗小鼠腹泻作用的研究[J].中国微生态学杂志,2009,21(4):323-326
[16]周洁.杨梅果实提取物的体内抑菌性研究及其主要活性成分的分离鉴定[D].无锡:江南大学,2009:13-16
[17]丁慧,胡龙华,钟桥石,杭亚平,刘衍伶,俞凤,陈艳慧,胡晓彦,张黎明,章白苓,张楠,王小中. 2015年临床常见分离菌分布及耐药特性分析[J].中国抗生素杂志,2017,42(11):967-973
[18]商军,顾欣,张文刚,姜芹,孙冰清.猪、鸡源分离粪肠球菌的核糖体分型及耐药性分析[J].中国抗生素杂志,2017,42(3):230-236
[19]聂荣,刘素纯,王建新.浸泡型杨梅酒花色苷稳定性的研究[J].酿酒科技,2014(5):56-58
[20]秦红,宋庆庆,华晓燕.杨梅果酒对肠道细菌的抑菌效果[J].贵州农业科学,2012,40(10):157-159
[21]徐岩,范文来,葛向阳,黄永光.科学认识中国白酒中的生物活性成分[J].酿酒科技,2013(9):1-6
[22] Qian J,Zhao W,Miao X,Li L,Zhang D. Sam68 modulatesapoptosis of intestinal epithelial cells via mediating NF-κB activationin ulcerative colitis[J]. Molecular Immunology,2016,75:48-59
[23] Dou W,Zhang J,Ren G,Ding L,Sun A,Deng C,Wu X,Wei X,Mani S,Wang Z. Mangiferin attenuates the symptoms of dextransulfate sodium-induced colitis in mice via NF-κB and MAPKsignaling inactivation[J]. International Immunopharmacology,2014,23(1):170-178
[24] Daniele S,Da Pozzo E,Zappelli E,Martini C. Trazodone treatmentprotects neuronal-like cells from inflammatory insult by inhibitingNF-κB,p38 and JNK[J]. Cellular Signalling,2015,27(8):1609-1629
[25] Villalba M,Fredericksen F,Otth C,OlavarríaⅤH. Molecularcharacterization of the bovine IER3 gene:Down-regulation of IL-8by blocking NF-κB activity mediated by IER3 overexpression inMDBK cells infected with bovine viral diarrhea virus-1[J].Molecular Immunology,2017,92:169-179
[26] Uwada J,Yazawa T,Islam M T,Khan M,Krug S,Fromm M,Karaki SⅠ, Suzuki Y, Kuwahara A, Yoshiki H, Sada K,MuramatsuⅠ, Taniguchi T. Activation of muscarinic receptorsprevents TNF-α-mediated intestinal epithelial barrier disruptionthrough p38 MAPK[J]. Cellular Signalling,2017,35:188-196
[27] Jeong C H, Seok J S, Petriello M C, Han S G. Arsenicdownregulates tight junction claudin proteins through p38 andNF-κB in intestinal epithelial cell line,HT-29[J]. Toxicology,2017,379:31-39
[28] Guo H,Zhong R,Liu Y,Jiang X,Tang X,Li Z,Xia M,Ling W.Effects of bayberry juice on inflammatory and apoptotic markers inyoung adults with features of non-alcoholic fatty liver disease[J].Nutrition,2014,30(2):198-203
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