通过式高效净化-超高效液相色谱-串联质谱测定猪肉、猪肝及其脂肪组织中21种三唑类杀菌剂

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英文篇名：Detection of Twenty-one Triazole Fungicides in Pork,Pork Liver and Pork Fat by Going Through Column Purification and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry 作者：李晓丹 ; 汪建妹 ; 王向军 ; 杨华 ; 吉小凤 ; 徐杰 ; 钱鸣蓉 英文作者：LI Xiao-Dan;WANG Jian-Mei;WANG Xiang-Jun;YANG Hua;JI Xiao-Feng;XU Jie;QIAN Ming-Rong;College of Forestry and Bio-technology,Zhejiang Agricultural and Forestry University;Institute of Quality and Standard for Agro-products,Zhejiang Academy of Agricultural Sciences; 关键词：超高效液相色谱-串联质谱 ; 猪肉 ; 猪肝 ; 猪脂肪 ; 三唑类杀菌剂 英文关键词：Ultra performance liquid chromatography-tandem mass spectrometry;;Pork;;Pork liver;;Pork fat;;Triazole fungicides 中文刊名：FXHX 英文刊名：Chinese Journal of Analytical Chemistry 机构：浙江农林大学林业与生物技术学院;浙江省农业科学院农产品质量标准研究所; 出版日期：2019-02-15 出版单位：分析化学 年：2019 期：v.47 基金：农业行业标准制定项目(No.2016R19A24C08);; 国家风险评估项目(No.GJFP2018007)资助~~ 语种：中文; 页：FXHX201902020 页数：9 CN：02 ISSN：22-1125/O6 分类号：138-146

摘要

建立了猪肉、猪肝及其脂肪组织中21种三唑类杀菌剂的超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。样品经乙腈提取,浓缩后加水混匀,经PRiME HLB柱净化,收集流出液,用UPLC-MS/MS法测定,基质标准曲线定量。21种三唑类杀菌剂在0.5～100μg/L浓度范围内线性良好,相关系数均大于0.99;在3个添加浓度(2.0、5.0和10. 0μg/kg)下,各杀菌剂的回收率为69. 5%～109. 5%,相对标准偏差为0. 4%～10.8%。此方法前处理操作简便,灵敏度高,精密度好,能较大程度除去基质干扰,可用于猪肉、猪肝及其脂肪组织中21种三唑类杀菌剂残留的测定。
        A ultra performance liquid chromatography-tandem mass spectrometry method for determination of triazole fungicides in pork,pork liver and pork fat tissue was established. Samples were extracted with acetonitrile,concentrated,and well-mixed with water. The mixed extract was then purified by a PRiME HLB column,and the elution was collected and detected by ultra performance liquid chromatography-tandem mass spectrometry. The matrix-matched standard curve was applied for quantification. The curve was linear in the range of 0.5-100 μg/L with the correlation coefficients of higher than 0.99. The recoveries ranged from 69.5% to 109.5% at the spiked levels of 2.0 μg/kg,5.0 μg/kg and 10.0 μg/kg,with relative standard deviations of 0.4%-10.8%. The method has many advantages such as simple operation,high sensitivity and good precision,and can largely eliminate the interference in the matrix and is suitable for the residue detection of 21 triazolecompounds in the muscle,liver and fat tissues of animal derived food.

引文

1 Reeves R,Thiruchelvam M,Richfield E K,Cory-Slechta,D.A.Pharmacol.Biochem.Behavior,2003,76(2):315-326
    2 HU Yan-Yun,XU Hui-Qun,YAO Jian,LYa-Ning,SONG Wei,LI Wen-Jing,HAN Fang,ZHENG Ping.Chinese J.Anal.Chem.,2014,42(2):227-232胡艳云,徐慧群,姚剑,吕亚宁,宋伟,李文静,韩芳,郑平.分析化学,2014,42(2):227-232
    3 LI Ji-Ge,WANG Yu-Fei,SHIJia-Wei,JIANG Li,YAO Xun-Ping,FANG Lan-Yun.Chinese Journal of Health Laboratory Technology,2013,(6):1345-1349李继革,王玉飞,施家威,蒋丽,姚浔平,方兰云.中国卫生检验杂志,2013,(6):1345-1349
    4 ZHANG Wei-Guo,GAO Jin-Shan,CHEN Shan-Shan,LI Chong-Jiu.Chinese J.Anal.Chem.,2005,33(10):1442-1444张伟国,高金山,陈姗姗,李重九.分析化学,2005,33(10):1442-1444
    5 Li Y B,Dong F S,Liu X,Xu J,Li J,Kong Z,Chen X,Liang X,Zheng Y.J.Chromatogr.A,2012,1224:51-60
    6 SUN Rui-Juan,GUO Jing,SONG Qian-Qian,SONG Wen-Hua.Acta Scientiarum Naturalium Universitatis Nankaiensis,2013,46(1):83-87孙瑞娟,郭晶,宋千千,宋文华.南开大学学报(自然科学版),2013,46(1):83-87
    7 Mistry of Health,Labour and Welfare.Positive List System for Agricultural Chemical Residues in Foods.(2006-5-29)[2018-10-22].http://www.ffcr.or.jp/zaidan/FFCRHOME.nsf/pages/MRLs-p
    8 Codex Alimentarius Commission Pesticide Residues in Food and Feed.(2016-07-01)[2018-10-22]http://www.fao.org/faowho-codexalimentarius//standards/pestres/pesticide-detail/en/?p_id=202
    9 GB2763-2016,National Food Safety Standard-Maximum Residue Limits for Pesticides in Food.National Standards of the People's Republic of China食品安全国家标准-食品中农药最大残留限量.中华人民共和国国家标准.GB 2763-2016
    10 HUANG Chao-Qun,DING Hui-Ying,ZHU Xiao-Yu,CHEN Ling-Ling,LI Bo,LOU Cheng-Jie.Journal of Instrumental Analysis,2011,30(6):656-660黄超群,丁慧瑛,朱晓雨,陈玲玲,李铂,楼成杰.分析测试学报,2011,30(6):656-660
    11 YAN Hong-Fei,LI Yong-Jun,WANG Mei-Ling,DAI Hua,ZHANG Ying,HUANG Zhi-Qiang.Analysis and Testing Technology and Instruments,2012,18(4):204-207颜鸿飞,李拥军,王美玲,戴华,张莹,黄志强.分析测试技术与仪器,2012,18(4):204-207
    12 WANG Fei,LI Tong,MA Chen.Chinese Journal of Chromatography,2013,31(3):191-199王菲,李彤,马辰.色谱,2013,31(3):191-199
    13 GE Na,LIU Xiao-Mao,LI Xue-Min,HUANG Xue-Zhe,WU Yan-Ping,LI Jin,LIU Yong-Ming.Journal of Instrumental Analysis,2011,30(12):1351-1355葛娜,刘晓茂,李学民,黄学者,吴艳萍,李金,刘永明.分析测试学报,2011,30(12):1351-1355
    14 WU Li,CHEN Ming-Xue,MU Ren-Xiang,YING Xing-Hua,CAO Zhao-Yun.Journal of Instrumental Analysis,2009,28(7):846-854吴俐,陈铭学,牟仁祥,应兴华,曹赵云.分析测试学报,2009,28(7):846-854
    15 LI Ji-Ge,WANG Yu-Fei,SHI Jia-Wei,JIANG Li,YAO Xun-Ping,FANG Lan-Yun.Chinese Journal of Chromatography,2012,30(3):262-266李继革,王玉飞,施家威,蒋丽,姚浔平,方兰云.色谱,2012,30(3):262-266
    16 WANG Hong-Bin,ZHAO Tian-Tian,LI Gui-Zhen,TAN Wei,LI Bin,YANG Min.Environmental Pollution&Control,2015,37(4):42-46王红斌,赵田甜,李桂镇,谭伟,李彬,杨敏.环境污染与防治,2015,37(4):42-46
    17 LIU Bo,YIN Hang,GAO Wen-Hui.Journal of Hebei University of Science and Technology,2013,34(5):417-421刘博,尹航,高文惠.河北科技大学学报,2013,34(5):417-421
    18 GUO Ya-Yun,DING Yan,HAN Xiao-Mei,WANG Zhe,SHI Hong-Mei.Journal of Analytical Science,2018,34(3):409-412郭亚芸,丁燕,韩晓梅,王哲,史红梅.分析科学学报,2018,34(3):409-412
    19 Pastor-Belda M,Garrido I,Campillo N,Vi1as P,Hellín P,Flores P.Food Chem.,2017,233:69-76
    20 GB23200.49-2016,National Food Safety Standards-Determination of Difenoconazole Residue in FOODS Gas Chromatography-Mass Spectrometry.National Standards of the People’s Republic of China食品安全国家标准-食品中苯醚甲环唑残留量的测定气相色谱-质谱法.中华人民共和国国家标准.GB 23200.49-2016
    21 GB 23200.53-2016,National Food Safety Standards-Determination of Flusilazole Residue in Foods Gas Chromatography-Mass Spectrometry.National Standards of the People’s Republic of China食品安全国家标准-食品中氟硅唑残留量的测定气相色谱-质谱法.中华人民共和国国家标准.GB 23200.53-2016
    22 SN/T 3935-2014.Determination of Uniconazole Class of Plant Growth Regulators Residue in Foods of export-GC-MS method.People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard出口食品中烯效唑类植物生长调节剂残留量的测定气相色谱-质谱法.中华人民共和国出入境检验检疫行业标准.SN/T 3935-2014
    23 Souza R,Pareja L,Cesio M V,Heinzen H.Chromatographia,2016,79(17-18):1-12
    24 LV Bing,CHEN Da-Wei,MIAO Hong.Journal of Instrumental Analysis,2015,34(6):639-645吕冰,陈达炜,苗虹.分析测试学报,2015,34(6):639-645
    25 Hong Y H,Xu X L,Li W Q,Xu B Z,Wu H Q,Cheng Y,Chen H,Zhang B S,Zhang F.Anal.Methods,2017,9(2):6534-6548
    26 WANG Zhi,SHI Zong-Wei,XI Cun-Xian,CAO Shu-Rui,WANG Guo-Min,TANG Bai-Bin,ZHENG Jia,MU Zhao-De.Journal of Instrumental Analysis,2017,36(10):1219-1223王智,施宗伟,郗存显,曹淑瑞,王国民,唐柏彬,郑佳,母昭德.分析测试学报,2017,36(10):1219-1223
    27 Matuszewski B K,Constanzer M L,Chavez-Eng C M.Anal.Chem.,2003,75(13):3019-3030