肥胖复合牙周炎建模相关体重丢失的不对称问题
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摘要
目的:探索肥胖复合牙周炎建模的主要实验因素对结扎后体重丢失(POWL)的影响。方法:建立饮食诱导型肥胖与结扎诱导牙周炎的小鼠复合模型。四因素方差分析判别饲料期(8、16、30周)、饲料、结扎、结扎期(5、10d)对POWL的效应。结果:肥胖小鼠、牙周炎小鼠的POWL比各自对照组分别高70%(10.4%vs.6.1%,P<0.001)、30%(9.6%vs.7.4%,P=0.002)。随着肥胖程度增加,肥胖小鼠的POWL不断加重(P<0.001),与对照组的POWL差距拉大,且丢失体重恢复更困难。在特定饲料期(16周),POWL随结扎期延长而加重。结论:建立肥胖复合牙周炎模型时,POWL在主要实验因素(肥胖及其程度、牙周炎及其程度)的组内分布均不对称,应慎重评估并弱化其对实验目标的潜在干扰。
Objective:To explore the effects of main experimental factors on post-operative weight loss(POWL)when establishing combined obesity and periodontitis models.Methods:Combined models of diet-induced obesity and ligation-induced periodontitis were established.Effects of feeding duration(8,16 and 30weeks),diet,ligation and ligation duration(5and 10days)on POWL were evaluated by 4-way ANOVA.Results:DIO and periodontitits mice had 70%(10.4%vs.6.1%,P<0.001)and 30%(9.6%vs.7.4%,P=0.002)higher POWL,respectively,in contrast to their corresponding controls.As severity of obesity increased,POWL of the DIO mice was elevated and more difficult to recover and POWL difference between DIO mice and the controls was further enhanced.More POWL went with longer ligation duration after specific feeding duration(16weeks).Conclusion:When establishing combined obesity and periodontitis models,imbalanced POWL occurs within groups of the main experimental factors(including obesity and periodontitis and their severity),which should be critically assessed to minimize its potential disturbance on experimental objectives.
Objective:To explore the effects of main experimental factors on post-operative weight loss(POWL)when establishing combined obesity and periodontitis models.Methods:Combined models of diet-induced obesity and ligation-induced periodontitis were established.Effects of feeding duration(8,16 and 30weeks),diet,ligation and ligation duration(5and 10days)on POWL were evaluated by 4-way ANOVA.Results:DIO and periodontitits mice had 70%(10.4%vs.6.1%,P<0.001)and 30%(9.6%vs.7.4%,P=0.002)higher POWL,respectively,in contrast to their corresponding controls.As severity of obesity increased,POWL of the DIO mice was elevated and more difficult to recover and POWL difference between DIO mice and the controls was further enhanced.More POWL went with longer ligation duration after specific feeding duration(16weeks).Conclusion:When establishing combined obesity and periodontitis models,imbalanced POWL occurs within groups of the main experimental factors(including obesity and periodontitis and their severity),which should be critically assessed to minimize its potential disturbance on experimental objectives.
引文
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[2]Khan S,Barrington G,Bettiol S,et al.Is overweight/obesity a risk factor for periodontitis in young adults and adolescents?a systematic review[J].Obes Rev,2018,19(6):852-883.
[3]Martinezherrera M,Silvestrerangil J,Silvestre FJ.Association between obesity and periodontal disease.A systematic review of epidemiological studies and controlled clinical trials[J].Med Oral Patol Oral Cir Bucal,2017,22(6):e708-e715.
[4]Wu C,Liu C,Luo K,et al.Changes in expression of the membrane receptors CD14,MHC-Ⅱ,SR-A,and TLR4in tissue-specific monocytes/macrophages following Porphyromonas gingivalis-LPS stimulation[J].Inflammation,2018,41(2):418-431.
[5]Muluke M,Gold T,Kiefhaber K,et al.Diet-induced obesity and its differential impact on periodontal bone loss[J].J Dent Res,2016,95(2):223-229.
[6]Yu T,Zhao L,Huang X,et al.Postoperative weight loss masks metabolic impacts of periodontitis in obese rodents[J].J Periodontol,2017,88(6):e97-e108.
[7]Huang X,Yu T,Ma C,et al.Macrophages play a key role in the obesity induced periodontal innate immune dysfunction via NLRP3pathway[J].J Periodontol,2016,87(10):1195-1205.
[8]Zuza EP,Garcia VG,Theodoro LH,et al.Influence of obesity on experimental periodontitis in rats:histopathological,histometric and immunohistochemical study[J].Clin Oral Investig,2018,22(3):1197-1208.
[9]Cavagni J,de Macedo IC,Gaio EJ,et al.Obesity and hyperlipidemia modulate alveolar bone loss in Wistar rats[J].J Periodontol,2016,87(2):e9-e17.
[10]余挺,赵莉,章锦才,等.超重小鼠应对牙周感染的血清炎症因子反应[J].口腔医学研究,2018,34(6):644-647.
[11]余挺,赵莉,章锦才,等.麻醉对肥胖复合牙周炎模型副作用的研究[J].口腔医学,2018,38(10):885-889.
[12]Yu T,Zhao L,Huang X,et al.Enhanced activity of the macrophage M1/M2phenotypes and phenotypic switch to M1in periodontal infection[J].J Periodontol,2016,87(9):1092-1102.
[13]Mahan L.Krause's Food,Nutrition&Diet Therapy[M].10th ed.Philadelphia:W.B.Saunders Co.,2000.
[14]Dobner J,Kaser S.Body mass index and the risk of infectionfrom underweight to obesity[J].Clin Microbiol Infect,2018,24(1):24-28.
[15]Ibrahim MK,Zambruni M,Melby CL,et al.Impact of childhood malnutrition on host defense and infection[J].Clin Microbiol Rev,2017,30(4):919-971.
[16]Kiekkas P,Stefanopoulos N,Bakalis N,et al.Perioperative adverse respiratory events in overweight/obese children:systematic review[J].J Perianesth Nurs,2016,31(1):11-22.
[17]Good DJ.Using obese mouse models in research:special considerations for IACUC members,animal care technicians,and researchers[J].Lab Anim(NY),2005,34(2):30-37.
[18]余挺,赵莉,章锦才,等.高脂软饲料增加肥胖复合牙周炎小鼠模型丝线松脱率的研究[J].口腔医学,2018,38(6):491-494.
[19]Franco NH,Correia-Neves M,Olsson IA.How“humane”is your endpoint?Refining the science-driven approach for termination of animal studies of chronic infection[J].PLoSPathog,2012,8(1):e1002399.
[20]Ni J,Chen L,Zhong S,et al.Influence of periodontitis and scaling and root planing on insulin resistance and hepatic CD36in obese rats[J].J Periodontol,2018,89(4):476-485.
[21]余挺.肥胖状态下牙周感染对宿主免疫反应的影响及机制初探[D].南方医科大学,2015.