紫檀芪对2型糖尿病大鼠学习记忆能力及海马氧化应激水平的影响
|
摘要
目的:探讨紫檀芪对2型糖尿病大鼠学习记忆能力及海马组织氧化应激水平的影响。方法:100只4周龄雄性SD大鼠适应性喂养1周后,选取10只作为正常对照组,其余大鼠给予高脂高糖饲料喂养。4周后夜间禁食12 h,按40 mg/kg剂量一次性空腹注射链脲佐菌素溶液,制备2型糖尿病大鼠模型。选取造模成功的大鼠40只分为2型糖尿病组、紫檀芪低剂量组、紫檀芪中剂量组、紫檀芪高剂量组,每组10只。紫檀芪干预7周后,采用全自动生化分析仪检测血糖和血清胰岛素,用Morris水迷宫测定大鼠学习记忆能力,用氧化酶试剂盒检测海马组织中SOD活性和MDA水平。结果:与正常对照组相比,2型糖尿病组大鼠血糖、血清胰岛素水平升高(P<0.05);与2型糖尿病组大鼠相比,紫檀芪低、中剂量组血糖降低,紫檀芪低、中、高剂量组血清胰岛素水平降低(P<0.05)。同时,与正常对照组相比,2型糖尿病组大鼠逃避潜伏期延长,穿越平台次数减少,海马组织SOD活性降低,MDA水平增加(P<0.05);与2型糖尿病组大鼠相比,紫檀芪低、中、高剂量组逃避潜伏期缩短,穿越平台次数增加,海马组织SOD活性升高,MDA水平降低(P<0.05)。结论:紫檀芪可以改善2型糖尿病大鼠学习记忆能力,提高海马组织SOD活性,对2型糖尿病大鼠认知障碍具有一定的改善作用。
Aim:To explore the effects of pterostilbene(PTE) on learning and memory ability and oxidative stress in hippocampus of type 2 diabetic rats.Methods:A total of 100 SD rats were randomly allocated into control group(n=10)and test group(n=90).The test group was for making type 2 diabetes model(T2DM),which was induced by combination of high-fat and high-sugar diet combined with streptozotocin(40 mg/kg).Then 40 T2DM rats were randomly allocated into model group,PTE low dose group,PTE middle dose group and PTE high dose group.After administration of PTE for 7 weeks,fasting blood glucose(FBG) and serum insulin(INS) were examined,the learning and memory performance were detected by Morris water maze,and the SOD activity and MDA level were evaluated.Results:Compared with normal rats,the levels of FBG and INS were increased in T2DM group(P<0.05),whereas the level of FBG in PTE(low and middle dose) groups was lower than that of T2DM group(P<0.05),the level of INS in PTE(low,middle and high dose) groups was lower than that of T2DM group(P<0.05).Compared with normal rats,the escape latency extended,the times across the platform shortened,the activity of SOD reduced,and the level of MDA augmented in T2DM group(P<0.05).Moreover,compared with T2DM rats,the escape latency diminished,the times across the platform increased,the activity of SOD augmented,and the level of MDA lessened in PTE(low,middle and high dose) groups(P<0.05).Conclusion:PTE could improve the learning and memory ability and increase SOD activity of type 2 diabetic rats,which might improve the cognitive decline.
Aim:To explore the effects of pterostilbene(PTE) on learning and memory ability and oxidative stress in hippocampus of type 2 diabetic rats.Methods:A total of 100 SD rats were randomly allocated into control group(n=10)and test group(n=90).The test group was for making type 2 diabetes model(T2DM),which was induced by combination of high-fat and high-sugar diet combined with streptozotocin(40 mg/kg).Then 40 T2DM rats were randomly allocated into model group,PTE low dose group,PTE middle dose group and PTE high dose group.After administration of PTE for 7 weeks,fasting blood glucose(FBG) and serum insulin(INS) were examined,the learning and memory performance were detected by Morris water maze,and the SOD activity and MDA level were evaluated.Results:Compared with normal rats,the levels of FBG and INS were increased in T2DM group(P<0.05),whereas the level of FBG in PTE(low and middle dose) groups was lower than that of T2DM group(P<0.05),the level of INS in PTE(low,middle and high dose) groups was lower than that of T2DM group(P<0.05).Compared with normal rats,the escape latency extended,the times across the platform shortened,the activity of SOD reduced,and the level of MDA augmented in T2DM group(P<0.05).Moreover,compared with T2DM rats,the escape latency diminished,the times across the platform increased,the activity of SOD augmented,and the level of MDA lessened in PTE(low,middle and high dose) groups(P<0.05).Conclusion:PTE could improve the learning and memory ability and increase SOD activity of type 2 diabetic rats,which might improve the cognitive decline.
引文
[1]刘畅,李明哲.2型糖尿病患者认知功能障碍影响因素研究[J].中国临床研究,2016,29(10):1362
[2]丁玎,洪震.老年性痴呆和轻度认知功能障碍的流行病学研究进展[J].中国临床神经科学,2013,21(1):101
[3]贺钰梅,汪丽丽,韩秀平,等.2型糖尿病患者合并认知功能障碍的危险因素分析[J].中国实用神经疾病杂志,2016,19(18):80
[4]MIKSTACKA R,RIMANDO AM,IGNATOWICZ E.Antioxidant effect of trans-resveratrol,pterostilbene,quercetin and their combinations in human erythrocytes in vitro[J].Plant Foods Hum Nutr,2010,65(1):57
[5]MCCORMACK D,MCFADDEN D.A review of pterostilbene antioxidant activity and disease modification[J].Oxid Med Cell Longev,2013,2013:575482
[6]PAUL S,RIMANDO AM,LEE HJ,et al.Anti-inflammatory action of pterostilbene is mediated through the p38 mitogen-activated protein kinase pathway in colon cancer cells[J].Cancer Prev Res(Phila),2009,2(7):650
[7]RIMANDOAM,NAGMANI R,FELLER DR,et al.Pterostilbene,a new agonist for the peroxisome proliferator-activated receptor alpha-isoform,lowers plasma lipoproteins and cholesterol in hypercholesterolemic hamsters[J].J Agric Food Chem,2005,53(9):3403
[8]PARI L,SATHEESH MA.Effect of pterostilbene on hepatic key enzymes of glucose metabolism in streptozotocin-and nicotinamide-induced diabetic rats[J].Life Sci,2006,79(7):641
[9]AMARNATH SATHEESH M,PARI L.The antioxidant role of pterostilbene in streptozotocin-nicotinamide-induced type2 diabetes mellitus in Wistar rats[J].J Pharm Pharmacol,2006,58(11):1483
[10]HSU CL,LIN YJ,HO CT,et al.Inhibitory effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells[J].Food Funct,2012,3(1):49
[11]刘丹丹,张伟,朱浩,等.Ⅱ型糖尿病大鼠动物模型的构建与验证[J].中国兽医学报,2016,36(7):1201
[12]张灵敏.黄秋葵水提物对实验性2型糖尿病大鼠血糖、血脂及血尿酸水平的影响[D].郑州:郑州大学,2016.
[13]张晓欢,孙贝贝,尹延彦,等.EGCG和维生素E对孕前期铅暴露仔鼠学习记忆能力的影响[J].郑州大学学报(医学版),2017,52(5):574
[14]刘欣欣,孙华磊,葛惠娜,等.紫檀芪对Ⅱ型糖尿病大鼠血脂的改善及对PPARγ的调控作用[J].现代食品科技,2018,34(9):6
[15]LUCHSINGER JA,REITZ C,PATEL B,et al.Relation of diabetes to mild cognitive impairment[J].Arch Neurol,2007,64(4):570
[16]宋珍.紫檀芪对神经细胞损伤模型的保护作用研究[D].济南:山东大学,2014.
[17]MA C,LONG H.Protective effect of betulin on cognitive decline in streptozocin(STZ)-induced diabetic rats[J].Neurotoxicology,2016,57:104
[18]赵晴,杜建时,刘松岩,等.依达拉奉对血管性痴呆大鼠海马组织SOD、MDA的影响[J].现代预防医学,2008,35(10):1950
[2]丁玎,洪震.老年性痴呆和轻度认知功能障碍的流行病学研究进展[J].中国临床神经科学,2013,21(1):101
[3]贺钰梅,汪丽丽,韩秀平,等.2型糖尿病患者合并认知功能障碍的危险因素分析[J].中国实用神经疾病杂志,2016,19(18):80
[4]MIKSTACKA R,RIMANDO AM,IGNATOWICZ E.Antioxidant effect of trans-resveratrol,pterostilbene,quercetin and their combinations in human erythrocytes in vitro[J].Plant Foods Hum Nutr,2010,65(1):57
[5]MCCORMACK D,MCFADDEN D.A review of pterostilbene antioxidant activity and disease modification[J].Oxid Med Cell Longev,2013,2013:575482
[6]PAUL S,RIMANDO AM,LEE HJ,et al.Anti-inflammatory action of pterostilbene is mediated through the p38 mitogen-activated protein kinase pathway in colon cancer cells[J].Cancer Prev Res(Phila),2009,2(7):650
[7]RIMANDOAM,NAGMANI R,FELLER DR,et al.Pterostilbene,a new agonist for the peroxisome proliferator-activated receptor alpha-isoform,lowers plasma lipoproteins and cholesterol in hypercholesterolemic hamsters[J].J Agric Food Chem,2005,53(9):3403
[8]PARI L,SATHEESH MA.Effect of pterostilbene on hepatic key enzymes of glucose metabolism in streptozotocin-and nicotinamide-induced diabetic rats[J].Life Sci,2006,79(7):641
[9]AMARNATH SATHEESH M,PARI L.The antioxidant role of pterostilbene in streptozotocin-nicotinamide-induced type2 diabetes mellitus in Wistar rats[J].J Pharm Pharmacol,2006,58(11):1483
[10]HSU CL,LIN YJ,HO CT,et al.Inhibitory effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells[J].Food Funct,2012,3(1):49
[11]刘丹丹,张伟,朱浩,等.Ⅱ型糖尿病大鼠动物模型的构建与验证[J].中国兽医学报,2016,36(7):1201
[12]张灵敏.黄秋葵水提物对实验性2型糖尿病大鼠血糖、血脂及血尿酸水平的影响[D].郑州:郑州大学,2016.
[13]张晓欢,孙贝贝,尹延彦,等.EGCG和维生素E对孕前期铅暴露仔鼠学习记忆能力的影响[J].郑州大学学报(医学版),2017,52(5):574
[14]刘欣欣,孙华磊,葛惠娜,等.紫檀芪对Ⅱ型糖尿病大鼠血脂的改善及对PPARγ的调控作用[J].现代食品科技,2018,34(9):6
[15]LUCHSINGER JA,REITZ C,PATEL B,et al.Relation of diabetes to mild cognitive impairment[J].Arch Neurol,2007,64(4):570
[16]宋珍.紫檀芪对神经细胞损伤模型的保护作用研究[D].济南:山东大学,2014.
[17]MA C,LONG H.Protective effect of betulin on cognitive decline in streptozocin(STZ)-induced diabetic rats[J].Neurotoxicology,2016,57:104
[18]赵晴,杜建时,刘松岩,等.依达拉奉对血管性痴呆大鼠海马组织SOD、MDA的影响[J].现代预防医学,2008,35(10):1950
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |