玉米低聚肽硒螯合物的体外抗氧化作用
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摘要
以玉米低聚肽和亚硒酸钠为原料经螯合工艺制得玉米低聚肽硒螯合物,对其水分、粗蛋白、酸溶蛋白、分子量分布情况进行了考察,然后从DPPH自由基、OH自由基清除能力和还原能力3方面对螯合原料和螯合物进行抗氧化功能对比与评价。结果表明,螯合得率为53.02%±0.17%,水分含量为7.46%±0.07%,酸溶蛋白占粗蛋白的比例为77.38%,分子量分布在1000u以下的比例超过85%。螯合原料中亚硒酸钠仅对OH自由基有较强清除能力;玉米低聚肽对DPPH自由基、OH自由基均有一定清除能力,且具有一定还原性;螯合物清除DPPH自由基、OH自由基能力及还原能力均比玉米低聚肽强,且具有剂量依赖。
Selenium-chelating corn oligopeptide were prepared by corn oligopeptide and sodium selenite. The properties of moisture, acid soluble protein, total nitrogen and molecular weight distribution were analyzed. Taking scavenging effects on DPPH, OH free radicals and reducing power as indicators, the antioxidant function of raw materials and chelates were evaluated and compared in vitro. The results showed that the yield was 53.02%±0.17%, the moisture content of the product was 7.46%±0.07%, the acid soluble protein accounted for 77.38% of the crude protein, and the molecular weight below 1000 u was more than 85%. In the raw materials, sodium selenite only had strong OH radicals scavenging ability.Corn oligopeptide had some scavenging ability on DPPH and OH radicals, and had some reducibility.Compared with corn oligopeptide, the selenium-chelating corn oligopeptide had better ability on scavenging DPPH and OH radicals, and reducing power, which were dose-dependent.
Selenium-chelating corn oligopeptide were prepared by corn oligopeptide and sodium selenite. The properties of moisture, acid soluble protein, total nitrogen and molecular weight distribution were analyzed. Taking scavenging effects on DPPH, OH free radicals and reducing power as indicators, the antioxidant function of raw materials and chelates were evaluated and compared in vitro. The results showed that the yield was 53.02%±0.17%, the moisture content of the product was 7.46%±0.07%, the acid soluble protein accounted for 77.38% of the crude protein, and the molecular weight below 1000 u was more than 85%. In the raw materials, sodium selenite only had strong OH radicals scavenging ability.Corn oligopeptide had some scavenging ability on DPPH and OH radicals, and had some reducibility.Compared with corn oligopeptide, the selenium-chelating corn oligopeptide had better ability on scavenging DPPH and OH radicals, and reducing power, which were dose-dependent.
引文
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[2] Bocco A,Cuvelier M E,Richard H,et al. Antioxidant activity and phenolic composition of citrus peel andseed extracts[J]. Journal of Agricultural&Food Chemistry,2015,46(6):2123-2129
[3]王征帆,杜艳,余莎,等.3种野菜提取物对羟基自由基清除率的实验研究[J].应用化工,2016,45(10):1849-1851
[4] Lin B, Yong-Chao Y U, Chen Y, et al. Effect of corn peptide on acute alcoholism in rats[J]. Food&Nutrition in China,2014
[5] Huang W H, Sun J, He H, et al. Antihypertensive effect of corn peptides, produced by a continuous production in enzymatic membrane reactor,in spontaneously hypertensive rats[J]. Food Chemistry,2011,128(4):968-973
[6] Liu D, Shi-Min L I, Zhang L J. Effect of corn antihypertensive peptide on ACE activity in vivo and in vitro[J]. Chinese Journal of Biochemical Pharmaceutics,2007
[7] Rayman M P. The importance of selenium to human health[J]. Lancet,2000,9225(356):233-241
[8] Shen H M, Yang C F, Ong C N. Sodium seleniteinduced oxidative stress and apoptosis in human hepatoma HepG 2, cells[J]. International Journal of Cancer,1999,81(5):820-828
[9]刘庆,田侠,史衍玺.外源硒矿粉对玉米硒累积及矿质元素吸收的影响[J].植物营养与肥料学报,2016,22(2):403-409
[10] Ahmad H, Tian J, Wang J, et al. Effects of dietary sodium selenite and selenium yeast on antioxidant enzyme activities and oxidative stability of chicken breast meat[J]. Journal of Agricultural&Food Chemistry,2012,60(29):7111-20
[11] GB 5009.3-2016,食品安全国家标准食品中水分的测定[S].2016
[12] GB 5009.5-2016,食品安全国家标准食品中蛋白质的测定[S].2016
[13] GB/T 22729-2008,海洋鱼低聚肽粉[S].2008
[14]刘文颖,林单,林峰,等.小麦低聚肽对HepG2细胞胆固醇代谢的影响[J].食品工业科技,2014,35(7):126-129
[15] Plis A. Application of free radical diphenylpicrylhydrazyl(DPPH)to estimate antioxidant capacity of food samples[J]. Analytical Methods,2013,17(5):4288-4295
[16]熊双丽,卢飞,史敏娟,等.DPPH自由基清除活性评价方法在抗氧化剂筛选中的研究进展[J].食品工业科技,2012,33(8):380-383
[17]吴道兵.SPATA12基因在氧化损伤中的作用及机制[D].长沙:湖南大学,2016
[18]徐霄龙,余进,刘涛,等.高温诱导氧化应激对大鼠小肠上皮细胞凋亡损伤的机制研究[C].中国畜牧兽医学会中兽医学分会2013年学术年会论文集,2013
[19]刘文颖,林峰,金振涛,等.玉米低聚肽的体外抗氧化作用[J].食品科学,2011,32(5):22-26
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