改进分子印迹固相萃取——高效液相色谱法同时测定辣椒制品中4种苏丹红含量
|
摘要
目的建立分子印迹固相萃取—高效液相色谱法同时测定辣椒制品中4种苏丹红(苏丹红Ⅰ、Ⅱ、Ⅲ和Ⅳ)的含量。方法改进GB/T19681-2005食品中苏丹红染料的检测方法高效液相色谱法中的试样处理方法,使用Welchrom~?SDH分子印迹固相萃取小柱对多种辣椒制品基质进行净化,并采用Ultimate~?XB-C18(250 mm×4.6 mm, 5μm)色谱柱,以乙腈和水为流动相进行等度洗脱,于500 nm波长下测定。结果苏丹红Ⅰ、Ⅱ、Ⅲ和Ⅳ在0.1～10.0μg/mL范围内线性关系良好,相关系数r~2为0.9995～0.9999,回收率为88.9%～106.6%,检出限为0.01 mg/kg。结论该方法简便、灵敏度高、准确性好,适用于食品中4种苏丹红的定量分析。
Objective To establish a method for simultaneous determination of 4 kinds of Sudan red in chili products by molecular imprinted solid phase extraction-high performance liquid chromatography. Methods GB/T 19681-2005 Method for the detection of Sudan red dyes in food-High performance liquid chromatography was improved, and various chili product substrates were purified using Welchrom~? SDH molecularly imprinted solid phase extraction column. Samples were separated with a Ultimate~? XB-C18(250 mm×4.6 mm, 5 μm) column by isocratic elution using acetonitrile and water as the mobile phase and detected at 500 nm. Results Sudan red I, II, III and IV had a good linear relationship in the range of 0.1-10.0 μg/mL, the correlation coefficients r~2 were 0.9995-0.9999, the recovery rates were 88.9%-106.6%, and the limit of detection was 0.01 mg/kg. Conclusion This method is simple, sensitive, accurate, and it is suitable for the quantitative analysis of 4 kinds of Sudan red in chili products sample at the same time.
Objective To establish a method for simultaneous determination of 4 kinds of Sudan red in chili products by molecular imprinted solid phase extraction-high performance liquid chromatography. Methods GB/T 19681-2005 Method for the detection of Sudan red dyes in food-High performance liquid chromatography was improved, and various chili product substrates were purified using Welchrom~? SDH molecularly imprinted solid phase extraction column. Samples were separated with a Ultimate~? XB-C18(250 mm×4.6 mm, 5 μm) column by isocratic elution using acetonitrile and water as the mobile phase and detected at 500 nm. Results Sudan red I, II, III and IV had a good linear relationship in the range of 0.1-10.0 μg/mL, the correlation coefficients r~2 were 0.9995-0.9999, the recovery rates were 88.9%-106.6%, and the limit of detection was 0.01 mg/kg. Conclusion This method is simple, sensitive, accurate, and it is suitable for the quantitative analysis of 4 kinds of Sudan red in chili products sample at the same time.
引文
[1]汪开银,熊晓辉,游京晶,等.辣椒制品中苏丹红Ⅰ快速检测的方法研究[J].生物加工过程,2018,16(2):63-68.Wang KY,Xiong XH,You JJ,et al.Rapid detection of Sudan redⅠin pepper products[J].Chin J Bioproc Eng,2018,16(2):63-68.
[2]程晓宏,杨俊,周楠,等.分子印迹固相萃取小柱-高效液相色谱法测定辣酱中4种苏丹红[J].理化检验-化学分册,2017,53(1):1336-1338.Cheng XH,Yang J,Zhou N,et al.Simultaneous determination of 4 SudanⅠ-Ⅳin chili sauce with molecular imprinted solid phase extraction and high performance liquid chromatography[J].Phys Test Chem Anal B,2017,53(1):1336-1338.
[3]StiborováM,Martínek V,RydlováH,et al.Expression of cytochrome P450 1A1 and its contribution to oxidation of a potential hunman carcinogen 1-phenylazo-2-naphthol(SudanⅠ)in human livers[J].Cancer Lett,2005,220(2):145-154.
[4]詹克航,时连.SPE-HPLC法测定辣椒及其制品中苏丹红色素含量[J].中国卫生检验杂志,2015,25(8):1169-1174.Zhan KH,Shi L.Detection of Sudan red colorants contents in chili and pepper-based products by SPE-HPLC[J].Chin J Health Lab Technol,2015,25(8):1169-1174.
[5]GB/T 2760-2014食品安全国标标准食品添加剂使用卫生标准[S].GB/T 2760-2014 National food safety standard-Hygienic standards for the use of food additives[S].
[6]刘正才,杨方,尹太坤,等.固相萃取/超高效液相色谱-串联质谱法同时测定禽蛋中4种苏丹红染料残留量[J].分析测试学报,2015,34(2):171-176.Liu ZC,Yang F,Yin TK,et al.Simultaneous determination of four Sudan red dyes residues in poultry eggs by solid-phase extraction combined with ultra performance liquid chromatography-tandem mass spectrometry[J].JInstrum Anal,2015,34(2):171-176.
[7]陈波.固相萃取-高效液相色谱法同时测定番茄酱中4种苏丹红染料[J].中国生化药物杂志,2016,36(10):152-154.Chen B.Simultaneous determination of four kinds of tonyred in ketchup by SPE-HPLC[J].Chin J Biochem Pharm,2016,36(10):152-154.
[8]朱浩,李小平,邹宝波,等.分散固相萃取/高效液相色谱法测定鸡蛋中对位红与苏丹红染料残留[J].分析测试学报,2013,32(11):1379-1383.Zhu H,Li XP,Zou BB,et al.Determination of para red and four Sudan dyes residues in eggs by dispersive solid-phase extraction combined with high performance liquid chromatography[J].J Instrum Anal,2013,32(11):1379-1383.
[9]陈静,刘召金,安宝超,等.全自动在线固相萃取-二维高效液相色谱与质谱联用测定辣椒油中苏丹红[J].分析化学,2013,41(9):1418-1422.Chen J,Liu ZJ,An BC,et al.Two-dimensional high performance liquid chromatography combined with on-line solid phase extraction and mass spectrometry for determination of Sudan dyes in chili oil[J].Chin J Anal Chem,2013,41(9):1418-1422.
[10]王重洋,王宁,吴琼,等.基质固相分散-超快速液相色谱测定山楂片中的4种苏丹红染料[J].分析化学,2014,42(4):597-601.Wang CY,Wang N,Wu Q,et al.Determination of four Sudan dyes in hawthorn tablets by matrix solid phase dispersion and ultra-fast liquid chromatography[J].Chin J Anal Chem,2014,42(4):597-601.
[11]陈煦,张絜青,王敏群,等.固相萃取-超高效液相色谱串联质谱测定辣椒中苏丹红染料方法研究[J].中国卫生检验杂志,2013,23(15):3047-3053.Chen X,Zhang JQ,Wang MQ,et al.Determination of Sudan dyes in chili products by solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry[J].Chin J Health Lab Technol,2013,23(15):3047-3053.
[12]He LM,Su YJ,Fang BH,et al.Determination of Sudan dye residues in eggs by liquid chromatography and gas chromatography-mass spectrometry[J].Anal Chim Acta,2007,594(1):139-146.
[13]Erdemir US,Izgi B,Gucer S.An alternative method for screening of Sudan dyes in red paprika paste by gas chromatography-mass spectrometry[J].Anal Method,2013,5(7):1790-1798.
[14]赵珊,张晶,丁晓静,等.凝胶净化/超高效液相色谱电喷雾质谱法检测调味油中11种禁用偶氮染料及罗丹明B[J].分析测试学报,2012,31(4):448-452.Zhao S,Zhang J,Ding XJ,et al.Determination of 11 azo-dyes and rhodamine B in seasoning oil by gel permeation chromatography/ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry[J].J Instrum Anal,2012,31(4):448-452.
[15]GB/T 19681-2005食品中苏丹红染料的检测方法高效液相色谱法[S].GB/T 19681-2005 Method for the determination of Sudan dyes in foods-High performance liquid chromatography[S].
[16]丁轶聪,王群,伊芳.对苏丹红GB/T 19681-2005检测方法的探讨与改进[J].化学分析计量,2010,19(4):53-55.Ding YC,Wang Q,Yi F.Discussion and improvement of GB/T19681-2005 examination method for Sudan red[J].Chem Anal Meter,2010,19(4):53-55.
[2]程晓宏,杨俊,周楠,等.分子印迹固相萃取小柱-高效液相色谱法测定辣酱中4种苏丹红[J].理化检验-化学分册,2017,53(1):1336-1338.Cheng XH,Yang J,Zhou N,et al.Simultaneous determination of 4 SudanⅠ-Ⅳin chili sauce with molecular imprinted solid phase extraction and high performance liquid chromatography[J].Phys Test Chem Anal B,2017,53(1):1336-1338.
[3]StiborováM,Martínek V,RydlováH,et al.Expression of cytochrome P450 1A1 and its contribution to oxidation of a potential hunman carcinogen 1-phenylazo-2-naphthol(SudanⅠ)in human livers[J].Cancer Lett,2005,220(2):145-154.
[4]詹克航,时连.SPE-HPLC法测定辣椒及其制品中苏丹红色素含量[J].中国卫生检验杂志,2015,25(8):1169-1174.Zhan KH,Shi L.Detection of Sudan red colorants contents in chili and pepper-based products by SPE-HPLC[J].Chin J Health Lab Technol,2015,25(8):1169-1174.
[5]GB/T 2760-2014食品安全国标标准食品添加剂使用卫生标准[S].GB/T 2760-2014 National food safety standard-Hygienic standards for the use of food additives[S].
[6]刘正才,杨方,尹太坤,等.固相萃取/超高效液相色谱-串联质谱法同时测定禽蛋中4种苏丹红染料残留量[J].分析测试学报,2015,34(2):171-176.Liu ZC,Yang F,Yin TK,et al.Simultaneous determination of four Sudan red dyes residues in poultry eggs by solid-phase extraction combined with ultra performance liquid chromatography-tandem mass spectrometry[J].JInstrum Anal,2015,34(2):171-176.
[7]陈波.固相萃取-高效液相色谱法同时测定番茄酱中4种苏丹红染料[J].中国生化药物杂志,2016,36(10):152-154.Chen B.Simultaneous determination of four kinds of tonyred in ketchup by SPE-HPLC[J].Chin J Biochem Pharm,2016,36(10):152-154.
[8]朱浩,李小平,邹宝波,等.分散固相萃取/高效液相色谱法测定鸡蛋中对位红与苏丹红染料残留[J].分析测试学报,2013,32(11):1379-1383.Zhu H,Li XP,Zou BB,et al.Determination of para red and four Sudan dyes residues in eggs by dispersive solid-phase extraction combined with high performance liquid chromatography[J].J Instrum Anal,2013,32(11):1379-1383.
[9]陈静,刘召金,安宝超,等.全自动在线固相萃取-二维高效液相色谱与质谱联用测定辣椒油中苏丹红[J].分析化学,2013,41(9):1418-1422.Chen J,Liu ZJ,An BC,et al.Two-dimensional high performance liquid chromatography combined with on-line solid phase extraction and mass spectrometry for determination of Sudan dyes in chili oil[J].Chin J Anal Chem,2013,41(9):1418-1422.
[10]王重洋,王宁,吴琼,等.基质固相分散-超快速液相色谱测定山楂片中的4种苏丹红染料[J].分析化学,2014,42(4):597-601.Wang CY,Wang N,Wu Q,et al.Determination of four Sudan dyes in hawthorn tablets by matrix solid phase dispersion and ultra-fast liquid chromatography[J].Chin J Anal Chem,2014,42(4):597-601.
[11]陈煦,张絜青,王敏群,等.固相萃取-超高效液相色谱串联质谱测定辣椒中苏丹红染料方法研究[J].中国卫生检验杂志,2013,23(15):3047-3053.Chen X,Zhang JQ,Wang MQ,et al.Determination of Sudan dyes in chili products by solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry[J].Chin J Health Lab Technol,2013,23(15):3047-3053.
[12]He LM,Su YJ,Fang BH,et al.Determination of Sudan dye residues in eggs by liquid chromatography and gas chromatography-mass spectrometry[J].Anal Chim Acta,2007,594(1):139-146.
[13]Erdemir US,Izgi B,Gucer S.An alternative method for screening of Sudan dyes in red paprika paste by gas chromatography-mass spectrometry[J].Anal Method,2013,5(7):1790-1798.
[14]赵珊,张晶,丁晓静,等.凝胶净化/超高效液相色谱电喷雾质谱法检测调味油中11种禁用偶氮染料及罗丹明B[J].分析测试学报,2012,31(4):448-452.Zhao S,Zhang J,Ding XJ,et al.Determination of 11 azo-dyes and rhodamine B in seasoning oil by gel permeation chromatography/ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry[J].J Instrum Anal,2012,31(4):448-452.
[15]GB/T 19681-2005食品中苏丹红染料的检测方法高效液相色谱法[S].GB/T 19681-2005 Method for the determination of Sudan dyes in foods-High performance liquid chromatography[S].
[16]丁轶聪,王群,伊芳.对苏丹红GB/T 19681-2005检测方法的探讨与改进[J].化学分析计量,2010,19(4):53-55.Ding YC,Wang Q,Yi F.Discussion and improvement of GB/T19681-2005 examination method for Sudan red[J].Chem Anal Meter,2010,19(4):53-55.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |