群体感应AHLs对大菱鲆体内温和气单胞菌致腐能力的影响
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摘要
将温和气单胞菌(AS7)及添加不同种类的AHLs信号分子标准品(C4-HSL、C6-HSL和C8-HSL)的菌液接种于大菱鲆无菌鱼块中,通过测定其在4℃冷藏过程中新鲜度指标(感官评定、菌落总数、pH、K值、持水性)的变化,研究群体感应对温和气单胞菌体内致腐能力的调控作用。结果表明,在贮藏期间添加C4-HSL和C8-HSL信号分子标准品,能够明显促进温和气单胞菌的生长,且对鱼块pH、K值、持水性及其品质变化影响较明显;而添加C6-HSL对其新鲜度指标均没有显著影响,说明群体感应可以通过AHLs来调控温和气单胞菌致腐能力。
The regulation of quorum sensing to the spoilage ability of Aeromonas sobria AS7 was studied by measuring the freshness indicators(sensory evaluation, total bacterial count, p H, K-value, water binding capacity) of turbot sterile fish blocks inoculated strains AS7 refrigerated at 4 ℃, contrasted to the samples adding different kinds of AHLs standard signal molecules. The results showed that adding C4-HSL and C8-HSL respectively showed significant correlation in different freshness index, but adding C6-HSL had no significant impact on the spoilage ability of bacteria. In conclusion, quorum sensing could regulate the spoilage ability of AS7 by AHLs.
The regulation of quorum sensing to the spoilage ability of Aeromonas sobria AS7 was studied by measuring the freshness indicators(sensory evaluation, total bacterial count, p H, K-value, water binding capacity) of turbot sterile fish blocks inoculated strains AS7 refrigerated at 4 ℃, contrasted to the samples adding different kinds of AHLs standard signal molecules. The results showed that adding C4-HSL and C8-HSL respectively showed significant correlation in different freshness index, but adding C6-HSL had no significant impact on the spoilage ability of bacteria. In conclusion, quorum sensing could regulate the spoilage ability of AS7 by AHLs.
引文
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[8] SHOBHARANI P, AGRAWAL R. Interception of quorum sensing signal molecule by furanone to en hance shelf life of fermented milk[J]. Food Control,2010, 21(1):61-69.
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[11]李婷婷,励建荣,赵崴.壳聚糖涂膜对冷藏美国红鱼品质的影响[J].食品科学, 2013, 34(10):299-303.
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[14]卫生部. GB18406.4-2001农产品安全质量:无公害水产品安全要求[S].北京:中国标准出版社, 2001.
[15]蓝蔚青,谢晶,杨胜平,等. Nisin生物保鲜剂对冷藏带鱼的保鲜效果研究[J].天然产物研究与开发,2010, 22(4):683-686.
[16] FAN W, SUN J, CHEN Y, et al. Effects of chitosan coating on quality and shelf life of silver carp during frozen storage[J]. Food Chemistry, 2009, 115(1):66-70.
[17]李婷婷,励建荣,胡文忠.可食性壳聚糖涂膜保鲜大黄鱼品质控制研究[J].中国食品学报, 2013, 13(6):147-152.
[18] OLAFSDOTTIR G, NESVADBA P, NATALE C D,et al. Multisensor for fish quality determination[J].Trends in Food Science&Technology, 2004, 15(2):86-93.
[19] WILKINSON R J, PATON N, PORTER M J R.The effects of pre-harvest stress and harvest method on the stress response, rigor onset, muscle pH and drip loss in barramundi(Lates calcarifer)[J]. Aquaculture, 2008, 282(1):26-32.
[20] BAHUAUD D, MORKORE T, LANGSRUD O, et al. Effects of-1.5℃super-chilling on quality of atlantic salmon(Salmo salar)prerigor fillets:cathepsin activity,muscle histology, texture and liquid leakage[J]. Food Chemistry, 2008, 111(2):329-339.
[21] ZHU S, WU H, ZENG M, et al. The involvement of bacterial quorum sensing in the spoilage of re frigerated Litopenaeus vannamei[J]. International Journal of Food Microbiology, 2015, 192:26-33.
[22] BRUHN J B, CHRISTENSEN A B, FLODGAARD L R, et al. Presence of acylated homoserine lactones(AHLs)and AHL-producing bacteria in meat and potential role of AHL in spoilage of meat[J].Applied and Environmental Microbiology, 2004, 70(7):4293-4302.
[2]綦国红,董明盛,陈晓红,等.鱼源假单胞菌群体感应信号分子与腐败特性相关关系的研究[J].中国农业科学, 2007, 40(7):1486-1491.
[3] FUQUA W C, WINANS S C, GREENBERG E P.Quorum sensing in bacteria:the LuxR-LuxI family of cell density-responsive transcriptional regulators[J].Journal of Bacteriology, 1994, 176(2):269.
[4] SOLANO C, ECHEVERZ M, LASA I. Biofilm dispersion and quorum sensing[J]. Current Opinion in Microbiology, 2014, 18:96-104.
[5] ANTUNES L C M, FERREIRA R B R, BUCKNER M M C, et al. Quorum sensing in bacterial virulence[J]. Microbiology, 2010, 156(8):2271-2282.
[6] RAVN L, CHRISTENSEN A B, MOLIN S, et al.Methods for detecting acylated homoserine lactones produced by gram-negative bacteria and their application in studies of ahl-production kinetics[J]. Journal of Microbiological Methods, 2001, 44(3):239-251.
[7] GRAM L, RAVN L, RASCH M, et al. Food spoilage-interactions between food spoilage bacteria[J].International Journal of Food Microbiology, 2002,78(1):79-97.
[8] SHOBHARANI P, AGRAWAL R. Interception of quorum sensing signal molecule by furanone to en hance shelf life of fermented milk[J]. Food Control,2010, 21(1):61-69.
[9] HERBERT R A, HENDRIE M S, GIBSON D M,et al. Bacteria active in the spoilage of certain sea foods[J]. Journal of Applied Microbiology, 2008, 34(1):41-50.
[10]李学英,杨宪时,郭全友,等.大黄鱼腐败菌腐败能力的初步分析[J].食品工业科技, 2009, 30(6):316-319.
[11]李婷婷,励建荣,赵崴.壳聚糖涂膜对冷藏美国红鱼品质的影响[J].食品科学, 2013, 34(10):299-303.
[12]卫生部. GB 4789.2-2010食品微生物学检验:菌落总数测定[S].北京:中国标准出版社, 2010.
[13] LI T T, LI J R, HU W Z, et al. Shelf-life extension of crucian carp(Carassius auratus)using natural preservatives during chilled storage[J]. Food Chemistry, 2012, 135(1):140-145.
[14]卫生部. GB18406.4-2001农产品安全质量:无公害水产品安全要求[S].北京:中国标准出版社, 2001.
[15]蓝蔚青,谢晶,杨胜平,等. Nisin生物保鲜剂对冷藏带鱼的保鲜效果研究[J].天然产物研究与开发,2010, 22(4):683-686.
[16] FAN W, SUN J, CHEN Y, et al. Effects of chitosan coating on quality and shelf life of silver carp during frozen storage[J]. Food Chemistry, 2009, 115(1):66-70.
[17]李婷婷,励建荣,胡文忠.可食性壳聚糖涂膜保鲜大黄鱼品质控制研究[J].中国食品学报, 2013, 13(6):147-152.
[18] OLAFSDOTTIR G, NESVADBA P, NATALE C D,et al. Multisensor for fish quality determination[J].Trends in Food Science&Technology, 2004, 15(2):86-93.
[19] WILKINSON R J, PATON N, PORTER M J R.The effects of pre-harvest stress and harvest method on the stress response, rigor onset, muscle pH and drip loss in barramundi(Lates calcarifer)[J]. Aquaculture, 2008, 282(1):26-32.
[20] BAHUAUD D, MORKORE T, LANGSRUD O, et al. Effects of-1.5℃super-chilling on quality of atlantic salmon(Salmo salar)prerigor fillets:cathepsin activity,muscle histology, texture and liquid leakage[J]. Food Chemistry, 2008, 111(2):329-339.
[21] ZHU S, WU H, ZENG M, et al. The involvement of bacterial quorum sensing in the spoilage of re frigerated Litopenaeus vannamei[J]. International Journal of Food Microbiology, 2015, 192:26-33.
[22] BRUHN J B, CHRISTENSEN A B, FLODGAARD L R, et al. Presence of acylated homoserine lactones(AHLs)and AHL-producing bacteria in meat and potential role of AHL in spoilage of meat[J].Applied and Environmental Microbiology, 2004, 70(7):4293-4302.
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