不同栽培品种高良姜HPLC指纹图谱研究及指标成分含量测定
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摘要
【目的】比较不同栽培品种高良姜(竹头姜与蜂窝姜)HPLC特征图谱,并测定3个指标成分的含量,为高良姜的品种鉴定、质量控制及优良品种的选育提供参考。【方法】采用高效液相色谱法(HPLC)测定样品指纹图谱,并对其结果进行相似度分析、聚类分析及主成分分析。同时测定二苯基庚烷A、高良姜素、山柰素的含量。【结果】不同栽培品种高良姜指纹图谱共有峰12个,但各组分比例差异显著。竹头姜与蜂窝姜之间的相似度为0.760～0.860,明显低于同品种高良姜之间的相似度。聚类分析和主成分分析均可将竹头姜与蜂窝姜样品分开。竹头姜与蜂窝姜中二苯基庚烷A、山柰素的平均含量较接近,而高良姜素的平均含量差异显著,分别为0.30%、0.98%。【结论】HPLC指纹图谱可用于区分不同栽培品种高良姜;指标成分含量测定方法可靠,重复性好,对高良姜栽培品种的鉴定、质量控制及品种选育具有指导意义和参考价值。
Objective To compare the HPLC fingerprint of different cultivars of Alpinia officinarum Hance(Zhutou galangal and Fengwo galangal) and to determine the contents of their 3 index components, so as to provide areference for the identification,quality control and breeding of excellent varieties of Alpinia officinarum Hance.Methods The fingerprints of the samples were determined by high performance liquid chromatography(HPLC),and the results were analyzed by similarity analysis, cluster analysis and principal component analysis. Thecontents of diphenylheptane A,galangin and kaempferid were simultaneously determined. Results There were 12 common peaks in the fingerprints of different cultivars of Alpinia officinarum Hance,but the proportions of eachcomponent were significantly different. The similarity between Zhutou galangal and Fengwo galangal was 0.760-0.860, which was significantly lower than the similarity among the samples of the same variety of Alpinia officinarum Hance. According to the results of both of the cluster analysis and the principal component analysis,Zhutou galangal can be identified from Fengwo galangal. The average contents of diphenylheptane A andkaempferid in Zhutou galangal were close to those of Fengwo galangal, while the average content of galanginbetween the two cultivars was significantly different, being 0.30%, 0.98% respectively. Conclusion HPLCfingerprint can be used to distinguish different cultivars of Alpiniao officinarum Hance. The method for determinationof index components is reliable and reproducible, which has guiding significance and reference value for theidentification,quality control and variety breeding of Alpinia officinarum Hance.
Objective To compare the HPLC fingerprint of different cultivars of Alpinia officinarum Hance(Zhutou galangal and Fengwo galangal) and to determine the contents of their 3 index components, so as to provide areference for the identification,quality control and breeding of excellent varieties of Alpinia officinarum Hance.Methods The fingerprints of the samples were determined by high performance liquid chromatography(HPLC),and the results were analyzed by similarity analysis, cluster analysis and principal component analysis. Thecontents of diphenylheptane A,galangin and kaempferid were simultaneously determined. Results There were 12 common peaks in the fingerprints of different cultivars of Alpinia officinarum Hance,but the proportions of eachcomponent were significantly different. The similarity between Zhutou galangal and Fengwo galangal was 0.760-0.860, which was significantly lower than the similarity among the samples of the same variety of Alpinia officinarum Hance. According to the results of both of the cluster analysis and the principal component analysis,Zhutou galangal can be identified from Fengwo galangal. The average contents of diphenylheptane A andkaempferid in Zhutou galangal were close to those of Fengwo galangal, while the average content of galanginbetween the two cultivars was significantly different, being 0.30%, 0.98% respectively. Conclusion HPLCfingerprint can be used to distinguish different cultivars of Alpiniao officinarum Hance. The method for determinationof index components is reliable and reproducible, which has guiding significance and reference value for theidentification,quality control and variety breeding of Alpinia officinarum Hance.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015.
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[3]Basri A M,Taha H,Ahmad N. A review on the pharmacological activities and phytochemicals of Alpinia officinarum(galangal)extracts derived from bioassay-guided fractionation and isolation[J]. Pharmacogn Rev,2017,11(21):43.
[4]刘华强.高良姜走势分析[J].中国现代中药,2011,13(12):63.
[5]阮薇儒,蒋林,陈昌胜,等.高良姜规范化生产标准操作规程(试行)[J].中国现代中药,2008,10(5):10.
[6]詹若挺,黄海波,潘超美,等.高良姜种植基地不同栽培类型的品种调查和形态比较研究[J].现代中药研究与实践,2007,21(2):3.
[7]袁源,林丽静,李积华,等.两种徐闻高良姜挥发油成分对比分析[J].中药材,2014,37(9):1613.
[8]罗焱,尤朋涛,杨敏,等.高良姜素诱导人乳腺癌细胞MCF-7凋亡的研究[J].中药新药与临床药理,2017,28(2):151.
[9] Ren K,Zhang W,Wu G,et al. Synergistic anti-cancer effects of galangin and berberine through apoptosis induction and proliferation inhibition in oesophageal carcinoma cells[J]. Biomed Pharmacother,2016,84:1748.
[10] Eumkeb G, Sakdarat S, Siriwong S. Reversingβ-lactam antibiotic resistance of Staphylococcus aureus with galangin from Alpinia officinarum Hance and synergism with ceftazidime[J].Phytomedicine,2010,18(1):40.
[11]陈晓素,杨晓凯,林一均,等.高良姜素对PC12细胞氧化损伤的保护作用及其机制研究[J].上海中医药大学学报,2016,30(3):93.
[12] Honmore V S,Kandhare A D,Kadam P P,et al. Isolates of Alpinia officinarum Hance as COX-2 inhibitors:Evidence from anti-inflammatory, antioxidant and molecular docking studies[J]. Int Immunopharmacol,2016,33:8.
[13] Luo Q,Zhu L,Ding J,et al. Protective effect of galangin in Concanavalin A-induced hepatitis in mice[J]. Drug Des Devel Ther,2015,9:2983.
[14] Yun C J,Mi E K,Ju H Y,et al. Anti-inflammatory effects of galangin on lipopolysaccharide-activated macrophages via ERK and NF-κB pathway regulation[J]. Immunopharmacol Immunotoxicol,2014,36(6):426.
[15]卢赣鹏. 500味常用中药材的经验鉴别[M].北京:中国中医药出版社,1999.
[16]冯丽娜,邓亦峰,梁念慈.高良姜中芳香类化合物的分离及其活性研究[J].时珍国医国药,2009,20(11):2732.
[2]杨全,严寒静,庞玉新,等.南药高良姜药用植物资源调查研究[J].广东药学院学报,2012,28(4):382.
[3]Basri A M,Taha H,Ahmad N. A review on the pharmacological activities and phytochemicals of Alpinia officinarum(galangal)extracts derived from bioassay-guided fractionation and isolation[J]. Pharmacogn Rev,2017,11(21):43.
[4]刘华强.高良姜走势分析[J].中国现代中药,2011,13(12):63.
[5]阮薇儒,蒋林,陈昌胜,等.高良姜规范化生产标准操作规程(试行)[J].中国现代中药,2008,10(5):10.
[6]詹若挺,黄海波,潘超美,等.高良姜种植基地不同栽培类型的品种调查和形态比较研究[J].现代中药研究与实践,2007,21(2):3.
[7]袁源,林丽静,李积华,等.两种徐闻高良姜挥发油成分对比分析[J].中药材,2014,37(9):1613.
[8]罗焱,尤朋涛,杨敏,等.高良姜素诱导人乳腺癌细胞MCF-7凋亡的研究[J].中药新药与临床药理,2017,28(2):151.
[9] Ren K,Zhang W,Wu G,et al. Synergistic anti-cancer effects of galangin and berberine through apoptosis induction and proliferation inhibition in oesophageal carcinoma cells[J]. Biomed Pharmacother,2016,84:1748.
[10] Eumkeb G, Sakdarat S, Siriwong S. Reversingβ-lactam antibiotic resistance of Staphylococcus aureus with galangin from Alpinia officinarum Hance and synergism with ceftazidime[J].Phytomedicine,2010,18(1):40.
[11]陈晓素,杨晓凯,林一均,等.高良姜素对PC12细胞氧化损伤的保护作用及其机制研究[J].上海中医药大学学报,2016,30(3):93.
[12] Honmore V S,Kandhare A D,Kadam P P,et al. Isolates of Alpinia officinarum Hance as COX-2 inhibitors:Evidence from anti-inflammatory, antioxidant and molecular docking studies[J]. Int Immunopharmacol,2016,33:8.
[13] Luo Q,Zhu L,Ding J,et al. Protective effect of galangin in Concanavalin A-induced hepatitis in mice[J]. Drug Des Devel Ther,2015,9:2983.
[14] Yun C J,Mi E K,Ju H Y,et al. Anti-inflammatory effects of galangin on lipopolysaccharide-activated macrophages via ERK and NF-κB pathway regulation[J]. Immunopharmacol Immunotoxicol,2014,36(6):426.
[15]卢赣鹏. 500味常用中药材的经验鉴别[M].北京:中国中医药出版社,1999.
[16]冯丽娜,邓亦峰,梁念慈.高良姜中芳香类化合物的分离及其活性研究[J].时珍国医国药,2009,20(11):2732.
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