水稻耐逆境种质创新研究十年回顾
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摘要
十多年来,我们围绕中国南方水稻生产遇到的主要逆境因素,开展了水稻种质挖掘和创新研究。克隆或优化了耐旱基因AtDREB1A和CbDREB1A,抗除草剂基因Epsps~#、Pat~#和Mat~#,抗螟虫基因Cry2Aa~#和Cry1Ca~#,以及植酸酶-牛乳铁蛋白肽融合基因PhyLf。培育出了抗除草剂早籼稻新种质Bar68,抗2种除草剂水稻新种质EB7001S,抗除草剂、具植酸酶和抗菌肽活性的水稻新种质BPL9K,抗螟虫、抗除草剂的水稻新种质B2A4008S、B2A68、B1C893和B1C106,以及一些耐旱、耐盐、耐低温并抗除草剂的水稻新种质。从爪哇稻、籼爪交重组自交系、粳稻中筛选出了一些耐高温、耐旱性较好的种质资源。改良了华占、岳恢9113、Y58S、广占63S等骨干杂交稻亲本的褐飞虱和白叶枯病抗性。培育出了耐涝中间材料Sub-1BS。这些耐逆境新种质的创制,将为水稻耐逆境育种提供有力的技术支撑。
Focusing on the dominant stresses of rice production in Southern China, we have carried out germplasm evaluation and innovation in rice for more than 10 years. A set of genes have been cloned or sequence-optimized, including the drought tolerance genes AtDREB1A and CbDREB1A, the herbicide resistance genes Epsps#, Pat# and Mat#, the lepidopteran resistance genes Cry2Aa~# and Cry1Ca~#, and the phytase-lactoferricin fusion gene PhyLf. The innovated rice germplasm covered Bar68, a glufosinate resistance indica rice variety for early cropping season; EB7001 S, a male sterile line with both glufosinate and glyphosate resistance; BPL9K, a glufosinate resistance rice variety as well as with both phytase and antibacterial activity in grains; B2A4008S, B2A68, B1C893 and B1C106, the rice varieties with both glufosinate resistance and lepidopteran resistance; and some glufosinate resistance rice varieties with drought tolerance, or with salinity tolerance, or with cold tolerance. Some rice germplasm with better thermal tolerance or drought tolerance were selected out from javanica rice resource, or from indica/javanica recombinant inbred lines, or from japonica rice resource. Some key parental lines of hybrid rice, including Huazhan, Yuehui9113, Y58S, and Guangzhan63 S etc., were improved in both brown planthopper resistance and bacterial blight resistance. A submergence tolerance line Sub-1 BS was developed by marker-assisted selection and twice submergence stress method. The evaluated and innovated rice germplasm as described above will provide strong support for future stress tolerance breeding in rice.
Focusing on the dominant stresses of rice production in Southern China, we have carried out germplasm evaluation and innovation in rice for more than 10 years. A set of genes have been cloned or sequence-optimized, including the drought tolerance genes AtDREB1A and CbDREB1A, the herbicide resistance genes Epsps#, Pat# and Mat#, the lepidopteran resistance genes Cry2Aa~# and Cry1Ca~#, and the phytase-lactoferricin fusion gene PhyLf. The innovated rice germplasm covered Bar68, a glufosinate resistance indica rice variety for early cropping season; EB7001 S, a male sterile line with both glufosinate and glyphosate resistance; BPL9K, a glufosinate resistance rice variety as well as with both phytase and antibacterial activity in grains; B2A4008S, B2A68, B1C893 and B1C106, the rice varieties with both glufosinate resistance and lepidopteran resistance; and some glufosinate resistance rice varieties with drought tolerance, or with salinity tolerance, or with cold tolerance. Some rice germplasm with better thermal tolerance or drought tolerance were selected out from javanica rice resource, or from indica/javanica recombinant inbred lines, or from japonica rice resource. Some key parental lines of hybrid rice, including Huazhan, Yuehui9113, Y58S, and Guangzhan63 S etc., were improved in both brown planthopper resistance and bacterial blight resistance. A submergence tolerance line Sub-1 BS was developed by marker-assisted selection and twice submergence stress method. The evaluated and innovated rice germplasm as described above will provide strong support for future stress tolerance breeding in rice.
引文
[1]肖国樱,陈芬,孟秋成,等.论湖南水稻育种的主攻方向和技术策略[J].杂交水稻,2015,30(4):1-5.Xiao G Y,Chen F,Meng Q C,et al.Key targets and technical strategy for rice breeding in Hunan Province[J].Hybrid Rice,2015,30(4):1-5.
[2]Farooq M,Siddique K H M,Rehman H,et al.Rice direct seeding:Experiences,challenges and opportunities[J].Soil and Tillage Research,2011,111(2):87-98.DOI:10.1016/j.still.2010.10.008.
[3]肖国樱,陈芬,孟秋成,等.我国转基因抗除草剂水稻的生态风险与控制[J].农业生物技术学报,2015,23(1):1-11.Xiao G Y,Chen F,Meng Q C,et al.Ecological risk and management of herbicide-resistant transgenic rice(Oryza sativa)in China[J].Journal of Agricultural Biotechnology,2015,23(1):1-11.
[4]Bzour M,Zuki F M,Mispan M S.Introduction of imidazolinone herbicide and Clearfield?rice between weedy rice-control efficiency and environmental concerns[J].Environmental Reviews,2018,26(2):181-198.http://dx.doi.org/10.1139/er-2017-0096.
[5]肖国樱.作物对除草剂的抗性及其在杂种优势中应用策略的探讨[J].杂交水稻,1997,12(5):1-3.Xiao G Y.The view of crop herbicide resistance for heterosis utilization[J].Hybrid Rice,1997,12(5):1-3.
[6]肖国樱,唐俐,袁定阳,等.转Bar基因抗除草剂两系杂交早稻恢复系Bar68-1的培育研究[J].杂交水稻,2007,22(6):57-61.Xiao G Y,Tang L,Yuan D Y,et al.Studies on development of Bar68-1,a Bar-transgenic restorer line with herbicide resistance of two-line early hybrid rice[J].Hybrid Rice,2007,22(6):57-61.
[7]Xiao G Y,Yuan L P,Sun S S M.Strategy and utilization of a herbicide resistance gene in two-line hybrid rice[J].Molecular Breeding,2007,20(3):287-292.
[8]肖国樱,邓力华.人工优化合成的Epsps基因与重组载体以及改变作物抗性的方法[P].中国,ZL201110270905.7.2015-01-14.Xiao G Y,Deng L H.Artificially optimized Epsps gene,recombinant vector and methods to enhance crop resistance[P].China,ZL201110270905.7.2015-01-14.
[9]Deng L H,Weng L S,Xiao G Y.Optimization of Epsps gene and development of double herbicide tolerant transgenic PGMSrice[J].Journal of Agricultural Science and Technology,2014,16(1):217-228.
[10]肖国樱,魏岁军,邓力华.核酸和检测转基因水稻EB7001S及其衍生系的方法以及试剂盒及其用途[P].中国,ZL201310362525.5.2018-04-13.Xiao G Y,Wei S J,Deng L H.Nucleic acid,method and kit for detecting transgenic rice EB7001S and its derived lines,as well as their application[P].China,ZL201310362525.5.2018-04-13.
[11]秦冠男,翁绿水,肖国樱.水稻密码子优化基因Mat#增强草铵膦抗性[J].农业生物技术学报,2016,24(10):1457-1465.Qin G N,Weng L S,Xiao G Y.The Mat#gene optimized by codon bias of rice(Oryza sativa)enhances resistance to glufosinate[J].Journal of Agricultural Biotechnology,2016,24(10):1457-1465.
[12]肖国樱,邓力华,翁绿水.人工优化合成的Pat#基因与重组载体以及改变作物抗性的方法[P].中国,201710093699.4.2017-06-13.Xiao G Y,Deng L H,Weng L S.Artificially optimized Pat#gene,recombinant vector and methods to enhance crop resistance[P].China,201710093699.4.2017-06-13.
[13]肖国樱,翁绿水,秦冠男.人工优化合成的Mat基因与重组载体以及改变作物抗性的方法[P].中国,201610213307.9.2016-04-07.Xiao G Y,Weng L S,Qin G N.Artificially optimized Mat gene,recombinant vector and methods to enhance crop resistance[P].China,201610213307.9.2016-04-07.
[14]Wang Z P,Deng L H,Weng L S,et al.Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity[J].Journal of Integrative Agriculture,2017,16(4):774-788.
[15]肖国樱,王作平,邓力华,等.人工合成的融合基因PhyLf与重组载体以及改良作物磷有效性和抗菌活性的方法[P].中国,201610549891.5.2016-7-14.Xiao G Y,Wang Z P,Deng L H,et al.Synthetic fusion gene Phy Lf,recombinant vector and methods to enhance grain phosphorus availability and antibacterial activity in crops[P].China,201610549891.5.2016-7-14.
[16]翁绿水,陈芬,肖国樱.Cry2Aa基因优化设计及功能验证[J].农业生物技术学报,2013,21(11):1261-1269.Weng L S,Chen F,Xiao G Y.Optimization and functional verification of Cry2Aa gene[J].Journal of Agricultural Biotechnology,2013,21(11):1261-1269.
[17]肖国樱,翁绿水.密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法[P].中国,ZL201210280832.4.2016-01-20.Xiao G Y,Weng L S.Codon optimization Cry2Aa gene,recombinant vector and methods to enhance crop resistance[P].China,ZL201210280832.4.2016-01-20.
[18]Weng L S,Deng L H,Lai X F,et al.Optimization of the Cry2Aa gene and development of insect-resistant and herbicide-tolerant photoperiod-sensitive genic male sterile rice[J].Czech Journal of Genetics and Plant Breeding,2014,50(1):19-25.
[19]翁绿水,蒋利平,肖国樱.抗虫抗除草剂转基因水稻恢复系B2A68的培育[J].杂交水稻,2013,28(1):63-67.Weng L S,Jiang L P,Xiao G Y.Development of an insect-resistant and herbicide-resistant transgenic restorer line B2A68 in rice[J].Hybrid Rice,2013,28(1):63-67.
[20]Liu W Q,Meng Q C,Weng L S,et al.A comparative study of twoline early season hybrid rice with lepidopteran resistance[J].Filed Crops Research,2016,187:107-112.
[21]蒋利平,翁绿水,肖国樱.转基因水稻B2A68事件特异性检测方法的建立[J].杂交水稻,2013,28(5):60-67.Jiang L P,Weng L S,Xiao G Y.Establishment of an event-specific method to detect transgenic rice B2A68[J].Hybrid Rice,2013,28(5):60-67.
[22]肖国樱,蒋利平,翁绿水.核酸和检测转基因水稻B2A68及其衍生系的方法以及试剂盒及其用途[P].中国,ZL201310068126.8.2017-05-03.Xiao G Y,Jiang L P,Weng L S.Nucleic acid,method and kit for detecting transgenic rice B2A68 and its derived lines,as well as their application[P].China,ZL201310068126.8.2017-05-03.
[23]翁绿水,王作平,肖国樱.转基因水稻B2A68中抗虫蛋白Cry2Aa的表达特征和抗性分析[J].农业生物技术学报,2018,26(5):756-763.Weng L S,Wang Z P,Xiao G Y.Expression profile of insecticidal protein Cry2Aa and lepidopteran resistance in transgenic rice(Oryza sativa)B2A68[J].Journal of Agricultural Biotechnology,2018,26(5):756-763.
[24]肖国樱,邓力华,邓晓湘,等.密码子优化的Cry1Ca#基因与重组载体以及改变作物抗性的方法[P].中国,ZL201310305677.1.2017-11-17.Xiao G Y,Deng L H,Deng X X,et al.Codon optimization Cry1Ca#gene,recombinant vector and methods to enhance crop resistance[P].China,ZL201310305677.1.2017-11-17.
[25]邓力华,邓晓湘,曹正春,等.抗虫抗除草剂转基因水稻B1C893的获得与鉴定[J].杂交水稻,2014,29(1):67-75.Deng L H,Deng X X,Cao Z C,et al.Development and identification of herbicide and insect resistant transgenic plant B1C893 in rice[J].Hybrid Rice,2014,29(1):67-75.
[26]肖国樱,魏岁军,邓力华.核酸和检测转基因水稻B1C893及其衍生系的方法以及试剂盒及其用途[P].中国,201610209349.5.2016-04-06.Xiao G Y,Wei S J,Deng L H.Nucleic acid,method and kit for detecting transgenic rice B1C893 and its derived lines,as well as their application[P].China,201610209349.5.2016-04-06.
[27]Hu W B,Deng X Y,Deng X X,et al.Characteristic analysis of tetra-resistant genetically modified rice[J].Journal of Integrative Agriculture,2018,17(3):493-506.
[28]杜洪伟,陈芬,肖国樱.拟南芥、荠菜DREB1A基因的克隆与植物表达载体的构建[J].生物技术通报,2008(4):99-103.Du H W,Chen F,Xiao G Y.Cloning of DREB1A gene from Arabidopsis thaliana and Capsella bursa-pastoris and construction of plant expression vector[J].Biotechnology Bulletin,2008(4):99-103.
[29]陈芬,杜洪伟,陈浩东,等.干旱响应转录因子DREB1A基因导入水稻光温敏核不育系的研究[J].生命科学研究,2008,12(1):24-28.Chen F,Du H W,Chen H D,et al.Studies on transformation of photo-sensitive and thermo-sensitive genic male sterile rice with dehydration responsive transcription factor gene DREB1A[J].Life Science Research,2008,12(1):24-28.
[30]陈浩东,罗伯祥,陈芬,等.CbDREB1A基因表达载体构建及转化水稻光温敏核不育系的研究[J].杂交水稻,2009,24(6):49-53.Chen H D,Luo B X,Chen F,et al.Construction of plant expression vector of CbDREB1A gene and its transformation into photoperiod-and thermo-sensitive genic male sterile line in rice[J].Hybrid Rice,2009,24(6):49-53.
[31]罗伯祥,邓力华,陈芬,等.OsbHLH1基因过表达载体构建及转化水稻研究[J].生物技术通报,2009(7):76-81.Luo B X,Deng L H,Chen F,et al.Construction of overexpression vector of OsbHLH1 gene and transformation of rice[J].Biotechnology Bulletin,2009(7):76-81.
[32]罗伯祥,肖自友,肖国樱.转OsbHLH1和Bar基因水稻及相关特性分析[J].农业生物技术学报,2012,20(1):30-37.Luo B X,Xiao Z Y,Xiao G Y.Transgenic rice with Bar and OsbHLH1 genes and its agronomic trait analyses[J].Journal of Agricultural Biotechnology,2012,20(1):30-37.
[33]罗伯祥,肖自友,邓力华,等.转山菠菜胆碱单加氧酶基因(AhCMO)水稻创制及其耐盐性研究[J].生命科学研究,2010,14(3):219-225.Luo B X,Xiao Z Y,Deng L H,et al.The development of transgenic rice with AhCMO gene and analysis of its salinity tolerance[J].Life Science Research,2010,14(3):219-225.
[34]金曦,罗伯祥,陈受宜,等.转BADH和Bar基因水稻培育及其相关特性评价[J].生命科学研究,2011,15(3):209-217.Jin X,Luo B X,Chen S Y,et al.Breeding and traits evaluation of BADH and Bar gene transgenic rice[J].Life Science Research,2011,15(3):209-217.
[35]Deng L X,Chen F,Jiang L P,et al.Ectopic expression of GmPAP3enhances salt tolerance in rice by alleviating oxidative damage[J].Plant Breeding,2014,133(3):348-355.
[36]Xiao Y L,Li J J,Yu J H,et al.Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza[J].Field Crops Research,2016,186:47-57.
[37]He C,Xiao Y L,Yu J H,et al.Pyramiding Xa21,Bph14,and Bph15 genes into the elite restorer line Yuehui9113 increases resistance to bacterial blight and the brown planthopper in rice[J].Crop Protection,2019,115:31-39.https://doi.org/10.1016/j.cropro.2018.09.001.
[38]肖友伦.聚合抗白叶枯病和抗褐飞虱基因的杂交稻亲本培育与相关性状研究[D].长沙:湖南农业大学,2016.Xiao Y L.Development of hybrid rice parental lines pyramiding bacterial blight and brown planthopper resistant genes and evaluation on related target traits of them[D].Changsha:Hunan Agricultural University,2016.
[39]樊庆鲁,郭加沅,肖国樱.水稻籼爪重组自交系群体芽期耐旱性鉴定[J].广西植物,2009,29(1):74-77.Fan Q L,Guo J Y,Xiao G Y.Drought tolerance of indica/javanica recombinant inbred line at germinating stage in rice[J].Guihaia,2009,29(1):74-77.
[40]周浩,胡文彬,王作平,等.抽穗扬花期高温对水稻重组自交系群体RIL47结实率的影响[J].中国生态农业学报,2011,19(1):69-74.Zhou H,Hu W B,Wang Z P,et al.Effect of high temperature at heading and flowering stage on seed-setting rate of rice recombinant inbreed line population(RIL47)[J].Chinese Journal of Eco-agriculture,2011,19(1):69-74.
[41]赵森,于江辉,周浩,等.抽穗开花期耐高温的爪哇稻资源筛选[J].植物遗传资源学报,2013,14(3):384-389.Zhao S,Yu J H,Zhou H,et al.Screening of javanica rice for thermo-tolerance at heading stage[J].Journal of Plant Genetic Resources,2013,14(3):384-389.
[42]于江辉,赵森,周浩,等.抽穗开花期高温对东北粳稻花粉育性和结实率的影响[J].湖南农业科学,2012(5):1-4,8.Yu J H,Zhao S,Zhou H,et al.Effect of high temperature at heading stage on pollen fertility and seed setting rate of japonica rice from Northeast China[J].Hunan Agricultural Sciences,2012(5):1-4,8.
[43]于江辉,赵森,周浩,等.分期播种对耐高温东北粳稻农艺性状的影响及耐热性评价[J].中国生态农业学报,2012,20(8):1037-1042.Yu J H,Zhao S,Zhou H,et al.Effect of interval sowing on agronomic traits and thermo-tolerance of japonica rice from Northeast China[J].Chinese Journal of Eco-Agriculture,2012,20(8):1037-1042.
[44]Li J J,Xiao Y L,Xiao G Y.Selection of submergence tolerant homozygous line by STS marker and twice submergence stress[J].Journal of Integrative Agriculture,2012,11(12):1940-1947.
[45]曾波,李爱宏,吕海霞.近年来中国水稻品种审定和推广应用的几个特点[J].湖北农业科学,2017,56(21):4035-4039,4055.Zeng B,Li A H,LüH X.Several features of approval and promotion of rice varieties in recent years in China[J].Hubei Agricultural Sciences,2017,56(21):4035-4039,4055.
[2]Farooq M,Siddique K H M,Rehman H,et al.Rice direct seeding:Experiences,challenges and opportunities[J].Soil and Tillage Research,2011,111(2):87-98.DOI:10.1016/j.still.2010.10.008.
[3]肖国樱,陈芬,孟秋成,等.我国转基因抗除草剂水稻的生态风险与控制[J].农业生物技术学报,2015,23(1):1-11.Xiao G Y,Chen F,Meng Q C,et al.Ecological risk and management of herbicide-resistant transgenic rice(Oryza sativa)in China[J].Journal of Agricultural Biotechnology,2015,23(1):1-11.
[4]Bzour M,Zuki F M,Mispan M S.Introduction of imidazolinone herbicide and Clearfield?rice between weedy rice-control efficiency and environmental concerns[J].Environmental Reviews,2018,26(2):181-198.http://dx.doi.org/10.1139/er-2017-0096.
[5]肖国樱.作物对除草剂的抗性及其在杂种优势中应用策略的探讨[J].杂交水稻,1997,12(5):1-3.Xiao G Y.The view of crop herbicide resistance for heterosis utilization[J].Hybrid Rice,1997,12(5):1-3.
[6]肖国樱,唐俐,袁定阳,等.转Bar基因抗除草剂两系杂交早稻恢复系Bar68-1的培育研究[J].杂交水稻,2007,22(6):57-61.Xiao G Y,Tang L,Yuan D Y,et al.Studies on development of Bar68-1,a Bar-transgenic restorer line with herbicide resistance of two-line early hybrid rice[J].Hybrid Rice,2007,22(6):57-61.
[7]Xiao G Y,Yuan L P,Sun S S M.Strategy and utilization of a herbicide resistance gene in two-line hybrid rice[J].Molecular Breeding,2007,20(3):287-292.
[8]肖国樱,邓力华.人工优化合成的Epsps基因与重组载体以及改变作物抗性的方法[P].中国,ZL201110270905.7.2015-01-14.Xiao G Y,Deng L H.Artificially optimized Epsps gene,recombinant vector and methods to enhance crop resistance[P].China,ZL201110270905.7.2015-01-14.
[9]Deng L H,Weng L S,Xiao G Y.Optimization of Epsps gene and development of double herbicide tolerant transgenic PGMSrice[J].Journal of Agricultural Science and Technology,2014,16(1):217-228.
[10]肖国樱,魏岁军,邓力华.核酸和检测转基因水稻EB7001S及其衍生系的方法以及试剂盒及其用途[P].中国,ZL201310362525.5.2018-04-13.Xiao G Y,Wei S J,Deng L H.Nucleic acid,method and kit for detecting transgenic rice EB7001S and its derived lines,as well as their application[P].China,ZL201310362525.5.2018-04-13.
[11]秦冠男,翁绿水,肖国樱.水稻密码子优化基因Mat#增强草铵膦抗性[J].农业生物技术学报,2016,24(10):1457-1465.Qin G N,Weng L S,Xiao G Y.The Mat#gene optimized by codon bias of rice(Oryza sativa)enhances resistance to glufosinate[J].Journal of Agricultural Biotechnology,2016,24(10):1457-1465.
[12]肖国樱,邓力华,翁绿水.人工优化合成的Pat#基因与重组载体以及改变作物抗性的方法[P].中国,201710093699.4.2017-06-13.Xiao G Y,Deng L H,Weng L S.Artificially optimized Pat#gene,recombinant vector and methods to enhance crop resistance[P].China,201710093699.4.2017-06-13.
[13]肖国樱,翁绿水,秦冠男.人工优化合成的Mat基因与重组载体以及改变作物抗性的方法[P].中国,201610213307.9.2016-04-07.Xiao G Y,Weng L S,Qin G N.Artificially optimized Mat gene,recombinant vector and methods to enhance crop resistance[P].China,201610213307.9.2016-04-07.
[14]Wang Z P,Deng L H,Weng L S,et al.Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity[J].Journal of Integrative Agriculture,2017,16(4):774-788.
[15]肖国樱,王作平,邓力华,等.人工合成的融合基因PhyLf与重组载体以及改良作物磷有效性和抗菌活性的方法[P].中国,201610549891.5.2016-7-14.Xiao G Y,Wang Z P,Deng L H,et al.Synthetic fusion gene Phy Lf,recombinant vector and methods to enhance grain phosphorus availability and antibacterial activity in crops[P].China,201610549891.5.2016-7-14.
[16]翁绿水,陈芬,肖国樱.Cry2Aa基因优化设计及功能验证[J].农业生物技术学报,2013,21(11):1261-1269.Weng L S,Chen F,Xiao G Y.Optimization and functional verification of Cry2Aa gene[J].Journal of Agricultural Biotechnology,2013,21(11):1261-1269.
[17]肖国樱,翁绿水.密码子优化的Cry2Aa基因与重组载体以及改变作物抗性的方法[P].中国,ZL201210280832.4.2016-01-20.Xiao G Y,Weng L S.Codon optimization Cry2Aa gene,recombinant vector and methods to enhance crop resistance[P].China,ZL201210280832.4.2016-01-20.
[18]Weng L S,Deng L H,Lai X F,et al.Optimization of the Cry2Aa gene and development of insect-resistant and herbicide-tolerant photoperiod-sensitive genic male sterile rice[J].Czech Journal of Genetics and Plant Breeding,2014,50(1):19-25.
[19]翁绿水,蒋利平,肖国樱.抗虫抗除草剂转基因水稻恢复系B2A68的培育[J].杂交水稻,2013,28(1):63-67.Weng L S,Jiang L P,Xiao G Y.Development of an insect-resistant and herbicide-resistant transgenic restorer line B2A68 in rice[J].Hybrid Rice,2013,28(1):63-67.
[20]Liu W Q,Meng Q C,Weng L S,et al.A comparative study of twoline early season hybrid rice with lepidopteran resistance[J].Filed Crops Research,2016,187:107-112.
[21]蒋利平,翁绿水,肖国樱.转基因水稻B2A68事件特异性检测方法的建立[J].杂交水稻,2013,28(5):60-67.Jiang L P,Weng L S,Xiao G Y.Establishment of an event-specific method to detect transgenic rice B2A68[J].Hybrid Rice,2013,28(5):60-67.
[22]肖国樱,蒋利平,翁绿水.核酸和检测转基因水稻B2A68及其衍生系的方法以及试剂盒及其用途[P].中国,ZL201310068126.8.2017-05-03.Xiao G Y,Jiang L P,Weng L S.Nucleic acid,method and kit for detecting transgenic rice B2A68 and its derived lines,as well as their application[P].China,ZL201310068126.8.2017-05-03.
[23]翁绿水,王作平,肖国樱.转基因水稻B2A68中抗虫蛋白Cry2Aa的表达特征和抗性分析[J].农业生物技术学报,2018,26(5):756-763.Weng L S,Wang Z P,Xiao G Y.Expression profile of insecticidal protein Cry2Aa and lepidopteran resistance in transgenic rice(Oryza sativa)B2A68[J].Journal of Agricultural Biotechnology,2018,26(5):756-763.
[24]肖国樱,邓力华,邓晓湘,等.密码子优化的Cry1Ca#基因与重组载体以及改变作物抗性的方法[P].中国,ZL201310305677.1.2017-11-17.Xiao G Y,Deng L H,Deng X X,et al.Codon optimization Cry1Ca#gene,recombinant vector and methods to enhance crop resistance[P].China,ZL201310305677.1.2017-11-17.
[25]邓力华,邓晓湘,曹正春,等.抗虫抗除草剂转基因水稻B1C893的获得与鉴定[J].杂交水稻,2014,29(1):67-75.Deng L H,Deng X X,Cao Z C,et al.Development and identification of herbicide and insect resistant transgenic plant B1C893 in rice[J].Hybrid Rice,2014,29(1):67-75.
[26]肖国樱,魏岁军,邓力华.核酸和检测转基因水稻B1C893及其衍生系的方法以及试剂盒及其用途[P].中国,201610209349.5.2016-04-06.Xiao G Y,Wei S J,Deng L H.Nucleic acid,method and kit for detecting transgenic rice B1C893 and its derived lines,as well as their application[P].China,201610209349.5.2016-04-06.
[27]Hu W B,Deng X Y,Deng X X,et al.Characteristic analysis of tetra-resistant genetically modified rice[J].Journal of Integrative Agriculture,2018,17(3):493-506.
[28]杜洪伟,陈芬,肖国樱.拟南芥、荠菜DREB1A基因的克隆与植物表达载体的构建[J].生物技术通报,2008(4):99-103.Du H W,Chen F,Xiao G Y.Cloning of DREB1A gene from Arabidopsis thaliana and Capsella bursa-pastoris and construction of plant expression vector[J].Biotechnology Bulletin,2008(4):99-103.
[29]陈芬,杜洪伟,陈浩东,等.干旱响应转录因子DREB1A基因导入水稻光温敏核不育系的研究[J].生命科学研究,2008,12(1):24-28.Chen F,Du H W,Chen H D,et al.Studies on transformation of photo-sensitive and thermo-sensitive genic male sterile rice with dehydration responsive transcription factor gene DREB1A[J].Life Science Research,2008,12(1):24-28.
[30]陈浩东,罗伯祥,陈芬,等.CbDREB1A基因表达载体构建及转化水稻光温敏核不育系的研究[J].杂交水稻,2009,24(6):49-53.Chen H D,Luo B X,Chen F,et al.Construction of plant expression vector of CbDREB1A gene and its transformation into photoperiod-and thermo-sensitive genic male sterile line in rice[J].Hybrid Rice,2009,24(6):49-53.
[31]罗伯祥,邓力华,陈芬,等.OsbHLH1基因过表达载体构建及转化水稻研究[J].生物技术通报,2009(7):76-81.Luo B X,Deng L H,Chen F,et al.Construction of overexpression vector of OsbHLH1 gene and transformation of rice[J].Biotechnology Bulletin,2009(7):76-81.
[32]罗伯祥,肖自友,肖国樱.转OsbHLH1和Bar基因水稻及相关特性分析[J].农业生物技术学报,2012,20(1):30-37.Luo B X,Xiao Z Y,Xiao G Y.Transgenic rice with Bar and OsbHLH1 genes and its agronomic trait analyses[J].Journal of Agricultural Biotechnology,2012,20(1):30-37.
[33]罗伯祥,肖自友,邓力华,等.转山菠菜胆碱单加氧酶基因(AhCMO)水稻创制及其耐盐性研究[J].生命科学研究,2010,14(3):219-225.Luo B X,Xiao Z Y,Deng L H,et al.The development of transgenic rice with AhCMO gene and analysis of its salinity tolerance[J].Life Science Research,2010,14(3):219-225.
[34]金曦,罗伯祥,陈受宜,等.转BADH和Bar基因水稻培育及其相关特性评价[J].生命科学研究,2011,15(3):209-217.Jin X,Luo B X,Chen S Y,et al.Breeding and traits evaluation of BADH and Bar gene transgenic rice[J].Life Science Research,2011,15(3):209-217.
[35]Deng L X,Chen F,Jiang L P,et al.Ectopic expression of GmPAP3enhances salt tolerance in rice by alleviating oxidative damage[J].Plant Breeding,2014,133(3):348-355.
[36]Xiao Y L,Li J J,Yu J H,et al.Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza[J].Field Crops Research,2016,186:47-57.
[37]He C,Xiao Y L,Yu J H,et al.Pyramiding Xa21,Bph14,and Bph15 genes into the elite restorer line Yuehui9113 increases resistance to bacterial blight and the brown planthopper in rice[J].Crop Protection,2019,115:31-39.https://doi.org/10.1016/j.cropro.2018.09.001.
[38]肖友伦.聚合抗白叶枯病和抗褐飞虱基因的杂交稻亲本培育与相关性状研究[D].长沙:湖南农业大学,2016.Xiao Y L.Development of hybrid rice parental lines pyramiding bacterial blight and brown planthopper resistant genes and evaluation on related target traits of them[D].Changsha:Hunan Agricultural University,2016.
[39]樊庆鲁,郭加沅,肖国樱.水稻籼爪重组自交系群体芽期耐旱性鉴定[J].广西植物,2009,29(1):74-77.Fan Q L,Guo J Y,Xiao G Y.Drought tolerance of indica/javanica recombinant inbred line at germinating stage in rice[J].Guihaia,2009,29(1):74-77.
[40]周浩,胡文彬,王作平,等.抽穗扬花期高温对水稻重组自交系群体RIL47结实率的影响[J].中国生态农业学报,2011,19(1):69-74.Zhou H,Hu W B,Wang Z P,et al.Effect of high temperature at heading and flowering stage on seed-setting rate of rice recombinant inbreed line population(RIL47)[J].Chinese Journal of Eco-agriculture,2011,19(1):69-74.
[41]赵森,于江辉,周浩,等.抽穗开花期耐高温的爪哇稻资源筛选[J].植物遗传资源学报,2013,14(3):384-389.Zhao S,Yu J H,Zhou H,et al.Screening of javanica rice for thermo-tolerance at heading stage[J].Journal of Plant Genetic Resources,2013,14(3):384-389.
[42]于江辉,赵森,周浩,等.抽穗开花期高温对东北粳稻花粉育性和结实率的影响[J].湖南农业科学,2012(5):1-4,8.Yu J H,Zhao S,Zhou H,et al.Effect of high temperature at heading stage on pollen fertility and seed setting rate of japonica rice from Northeast China[J].Hunan Agricultural Sciences,2012(5):1-4,8.
[43]于江辉,赵森,周浩,等.分期播种对耐高温东北粳稻农艺性状的影响及耐热性评价[J].中国生态农业学报,2012,20(8):1037-1042.Yu J H,Zhao S,Zhou H,et al.Effect of interval sowing on agronomic traits and thermo-tolerance of japonica rice from Northeast China[J].Chinese Journal of Eco-Agriculture,2012,20(8):1037-1042.
[44]Li J J,Xiao Y L,Xiao G Y.Selection of submergence tolerant homozygous line by STS marker and twice submergence stress[J].Journal of Integrative Agriculture,2012,11(12):1940-1947.
[45]曾波,李爱宏,吕海霞.近年来中国水稻品种审定和推广应用的几个特点[J].湖北农业科学,2017,56(21):4035-4039,4055.Zeng B,Li A H,LüH X.Several features of approval and promotion of rice varieties in recent years in China[J].Hubei Agricultural Sciences,2017,56(21):4035-4039,4055.
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