-Gx加速度对家兔肺组织基质金属蛋白酶9和基质金属蛋白酶抑制剂1水平的影响

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英文篇名：Effects of -Gx on MMP9 and TIMP1 expression in rabbit lung 作者：韩磊 ; 李鸣皋 ; 周家兴 ; 陈宇亮 ; 孙海文 英文作者：HAN Lei;LI Minggao;ZHOU Jiaxing;CHEN Yuliang;SUN Haiwen;Aviation and Nautical Medical Center,Navy General Hospital; 关键词：-Gx ; 肺脏 ; 基质金属蛋白酶9 ; 基质金属蛋白酶抑制剂1 ; 家兔 英文关键词：-Gx;;Lung;;Matrix metalloproteinase(MMP) 9;;Tissue inhibitor of metalloproteinase(TIMP) 1;;Rabbit 中文刊名：HJZY 英文刊名：Translational Medicine Journal 机构：海军总医院全军航海航空医学中心; 出版日期：2018-10-20 出版单位：转化医学杂志 年：2018 期：v.7;No.38 基金：全军十二五重大专项课题资助项目(AHJ11Z001) 语种：中文; 页：HJZY201805011 页数：4 CN：05 ISSN：10-1042/R 分类号：46-49

摘要

目的通过离体实验的方法,观察-Gx加速度作用对家兔肺脏组织基质金属蛋白酶(matrix metalloproteinase,MMP) 9和基质金属蛋白酶抑制剂(tissue inhibitor of metalloproteinase,TIMP) 1表达水平的影响,探索-Gx致肺损伤的作用机制。方法选取1月龄的雄性新西兰家兔20只,随机分为实验组和对照组,每组10只。实验组动物采用水平加速度试验平台进行-Gx加速度作用,加速度作用峰值3. 6 G,持续时间为2 s,每天进行加速度作用20次,间隔5 min,连续30 d。对照组动物不进行加速度作用,其他处理同实验组。采用免疫组化方法检测各组动物肺组织MMP9和TIMP1蛋白含量变化情况。结果 (1)对照组动物肺组织每高倍镜视野MMP9阳性细胞数为(13. 7±7. 2),-Gx暴露组动物每高倍镜视野阳性细胞数为(28. 3±12. 5),与对照组相比,-Gx暴露组动物肺组织的MMP9表达水平显著增高(P<0. 05);(2)对照组动物肺组织每高倍镜视野TIMP1阳性细胞数为(17. 4±10. 2),-Gx暴露组动物每高倍镜视野阳性细胞数为(8. 6±5. 9),与对照组相比,-Gx暴露组动物肺组织的TIMP1表达水平显著降低(P<0. 05)。结论 -Gx水平加速度作用可导致家兔肺组织MMP9和TIMP1表达水平的改变,其可能参与了-Gx加速度作用致肺损伤的发生发展过程。
        Objective To investigate the effects of-Gx on matrix metalloproteinase( MMP) 9 and tissue inhibitor of metalloproteinase( TIMP) 1 expression in rabbit lung. Methods Twenty male rabbits at 1 month old were divided into control group and-Gx group. The-Gx group rabbits were exposured by-Gx acceleration with peak of 3. 6 G and 2 s. Rabbits of-Gx group were exposured total 30 days and 20 times per day. The expression of MMP9 and TIMP1 were evalulated by immunohistochemical method. Results(1) The positive cell number of MMP9 in control group is( 13. 7±7. 2),that in-Gx group is( 28. 3±12. 5). The expression of MMP9 protein level in-Gx group rabbit is higher than that in control group( P<0. 05);(2)The positive cell number of TIMP1 in control group is( 17. 4±10. 2),while in-Gx group is( 8. 6±5. 9). The expression level of TIMP1 protein in-Gx group is lower than that of in control group( P<0. 05). Conclusion The MMP9 and TIMP1 expression in rabbit lung could be changed by-Gx action,which could be involed with the mechanism of lung injury induced by-Gx.

引文

[1]师绿江,尹昭云,洪欣,等.加速度致大鼠心肌细胞凋亡及金属硫蛋白变化[J].解放军预防医学杂志,2003,21(6):406-409.
    [2]Villalta PC,Rocic P,Townsley MI.Role of MMP2 in TRPV4-induced lung injury[J].Am J Physiol Lung Cell Mol Physiol,2014,307(8):L652-L659.
    [3]从金鹏,郝文嘉,宇文成.MMP9与TIMP1及其在慢性阻塞性肺疾病中的作用[J].青岛大学医学院学报,2015,51(2),241-243.
    [4]Evrosimovska B,Dimova C,Kovacevska I,et al.Concentration of collagenases MMP-1 in patients with chronically inflamed dental pulp tissue[J].Prilozi,2012,33(2):191-204.
    [5]姚豫桐,骆乐,李可洲,等.机械应力对人脐静脉内皮细胞MMP9 mRNA表达的调节[J].四川医学,2016,37(5):473-476.
    [6]Visse R,Nagase H. Matrix metalloproteinases and tissue inhibitors of metalloproteinases structure function and biochemistry[J].Crirc Res,2003,92(8):827-839.
    [7]王海波,俞为荣.MMP-2/MMP-9在炎症中的研究进展[J].医学综述,2014,20(17):3120-3122.
    [8]Guzel S,Serin O,Guzel EC,et al. Interleukin-33,matrix metalloproteinase-9,and tissue inhibitor[corrected]of matrix metalloproteinase-1 in myocardial infarction[J].Korean J Intern Med,2013,28(2):165-173.
    [9]Martinez LA,Galinanes EL.Matrix metalloproteinases and small artery remodeling[J]. Drug Discov Today Dis Models,2011,8(1):21-28.
    [10]于沛霞,薄立军,黄立宁,等.P38MAPK/MMP-9信号通路对新生大鼠脑缺氧缺血损伤的影响[J].河北医科大学学报,2017,38(9):1063-1067.
    [11]Nagy B,Woth G,Mérei,et al.Perioperative time course of matrix metalloproteinase-9(MMP-9),its tissue inhibitor TIMP-1&S100B protein in carotid surgery[J].Indian J Med Res,2016,143(2):220-226.
    [12]Lu Y,An J,Liu Y,et al.MMP9 is involved in HO-1-mediated upregulation of apical junctional complex in Caco-2cells under oxygen-glucose deprivation[J]. Biochem Biophys Res Commun,2018,498(1):125-131.
    [13]高娟,王添印,韩茹,等.MMP-9、TIMP-1在肺纤维化中作用的研究进展[J].山东医药,2017,57(26):104-106.
    [14]Zuo WL,Zhao JM,Huang JX,et al.Effect of bosentan is correlated with MM-9/TIMP-1 ratio in bleomycin-induced pulmonary fibrosis[J].Biomed Rep,2017,6(2):201-205.
    [15]Szymanowski K,Mikolajczyk M,Wirstlein P,et al.Matrix metalloptoteinase-2(MMP-2),MMP-9,tissue inhibitor of matrix metalloproteinases(TIMP-1)and transforming growth factor-β2(TGF-β2)expression in eutopic endometrium of women with peritoneal endometriosis[J]. Ann Agric Environ Med,2016,23(4):649-653.