小分子阿胶对小鼠免疫功能的影响
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摘要
目的研究小分子阿胶对小鼠免疫功能的调节作用。方法将ICR小鼠随机分为对照组、模型组、生血丸3.00 g/kg组和小分子阿胶3.00、1.50、0.75 g/kg组,ig给药14 d,ip环磷酰胺或氢化可的松建立免疫低下模型,用鸡红细胞诱导小鼠溶血素,测定小鼠血清溶血素水平,观察小分子阿胶对体液免疫的影响;采用二硝基氯苯诱导小鼠耳廓肿胀,检测小鼠耳廓肿胀度,观察小分子阿胶对细胞免疫的影响;利用流式细胞术对小鼠淋巴细胞亚群进行分析,观察小分子阿胶对小鼠淋巴细胞分型的影响。结果与模型组比较,小分子阿胶3.00、1.50、0.75 g/kg显著提高免疫功能低下小鼠血清溶血素含量(P<0.01);3.00、1.50 g/kg显著提高小鼠耳廓肿胀度(P<0.01、0.05);3.00 g/kg显著提高小鼠T淋巴细胞(CD3~+)、辅助性T细胞和迟发超敏性T细胞(CD3~+CD4~+)占淋巴细胞的比例(P<0.01)。结论小分子阿胶能提高小鼠体液免疫和细胞免疫功能,对免疫功能有正向调节作用。
Objective To study the effect of small molecular Asini Corii Colla(SMACC) on immune function in mice. Methods ICR mice were randomly divided into control group, model group, Shengxue Pills(3.00 g/kg) group, and SMACC(3.00, 1.50, 0.75 g/kg) group, and they were ig administered for 14 d. Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide or hydrocortisone. The use of red blood cells in chicken was to induce the production of hemolysin in mice. The content of serum hemolysin was measured to determine the effect of SMACC on humoral immunity. The ear swelling of mice was induced by two nitrochlorobenzene, and the auricular swelling of mice was detected to observe the effect of SMACC on cell immunity. Lymphocyte subsets in mice were analyzed by flow cytometry to observe the effect of SMACC on lymphocyte subtyping.Results Compared with model group, SMACC significantly increased the serum hemolysin content at doses of 3.00, 1.50, and 0.75 g/kg(P < 0.01), significantly increased the degree of auricular swelling in mice at doses of 3.00 and 1.50 g/kg(P < 0.01 and 0.05), and significantly promoted the proportion of T lymphocytes(CD3+), helper T cells, and delayed hypersensitivity T cells(CD3+CD4+) at doses of 3.00 g/kg(P < 0.01). Conclusions SMACC can enhance humoral immunity and cellular immune function in mice and has a positive regulatory effect on immune function in mice.
Objective To study the effect of small molecular Asini Corii Colla(SMACC) on immune function in mice. Methods ICR mice were randomly divided into control group, model group, Shengxue Pills(3.00 g/kg) group, and SMACC(3.00, 1.50, 0.75 g/kg) group, and they were ig administered for 14 d. Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide or hydrocortisone. The use of red blood cells in chicken was to induce the production of hemolysin in mice. The content of serum hemolysin was measured to determine the effect of SMACC on humoral immunity. The ear swelling of mice was induced by two nitrochlorobenzene, and the auricular swelling of mice was detected to observe the effect of SMACC on cell immunity. Lymphocyte subsets in mice were analyzed by flow cytometry to observe the effect of SMACC on lymphocyte subtyping.Results Compared with model group, SMACC significantly increased the serum hemolysin content at doses of 3.00, 1.50, and 0.75 g/kg(P < 0.01), significantly increased the degree of auricular swelling in mice at doses of 3.00 and 1.50 g/kg(P < 0.01 and 0.05), and significantly promoted the proportion of T lymphocytes(CD3+), helper T cells, and delayed hypersensitivity T cells(CD3+CD4+) at doses of 3.00 g/kg(P < 0.01). Conclusions SMACC can enhance humoral immunity and cellular immune function in mice and has a positive regulatory effect on immune function in mice.
引文
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[7]李敏,庞萌萌,田晨颖,等.不同阿胶酶解液相对分子量分布及补血升白作用对比研究[J].中国食品添加剂,2017,6:105-111.
[8]周娅红.人参总皂苷对免疫低下小鼠免疫功能的影响[J].中国中医急症,2010,19(9):1559-1561.
[9]曾永君,胡金芳,万梅绪,等.注射用益气复脉(冻干)对辐射所致小鼠免疫低下的改善作用[J].药物评价研究,2018,41(3):386-390.
[10]钟建春,张艳,丁振涛,等.人工冬虫夏草多糖提取物对小鼠免疫功能的影响[J].中山大学学报,2011,50(6):99-102.
[10]李覃,陈虹,白淑芳,等.青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用[J].中国组织工程研究,2012,16(5):843-846.
[11]安梦培,张守元,张淹,等.阿胶对免疫低下模型小鼠免疫功能的影响[J].药物评价研究,2018,41(4):567-571.
[12]Bellissimo F,Pinzone M R,Celesia B M,et al.Baseline CD4/CD8 T-cell ratio predicts prompt immune restoration upon c ART initiation[J].Curr HIV Res,2016,14(6):491-496.
[13]朱孝娟,孙玉薇,宋柏捷.大豆皂苷对荷瘤小鼠化疗疗效及T淋巴细胞亚群的影响[J].实用预防医学,2015,22(3):292-295.
[2]吴海燕,孙佳明,张辉.阿胶的研究进展[J].吉林中医药,2016,36(1):57-60.
[3]伊娜,杨铧,武勇,等.阿胶药理药效研究进展[J].世界最新医学信息文摘,2017,17(54):12-15.
[4]庞萌萌,李敏,田晨颖,等.阿胶酶解液相对分子质量分布及其补血升白作用[J].中国实验方剂学杂志,2017,23(12):13-17.
[5]付英杰.阿胶低肽及其制剂的研究[D].济南:山东中医药大学,2010.
[6]刘元涛,张惠惠,王升光,等.阿胶仿生酶解前后提高免疫力作用对比研究[J].时珍国医国药,2016,27(9):2158-2160.
[7]李敏,庞萌萌,田晨颖,等.不同阿胶酶解液相对分子量分布及补血升白作用对比研究[J].中国食品添加剂,2017,6:105-111.
[8]周娅红.人参总皂苷对免疫低下小鼠免疫功能的影响[J].中国中医急症,2010,19(9):1559-1561.
[9]曾永君,胡金芳,万梅绪,等.注射用益气复脉(冻干)对辐射所致小鼠免疫低下的改善作用[J].药物评价研究,2018,41(3):386-390.
[10]钟建春,张艳,丁振涛,等.人工冬虫夏草多糖提取物对小鼠免疫功能的影响[J].中山大学学报,2011,50(6):99-102.
[10]李覃,陈虹,白淑芳,等.青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用[J].中国组织工程研究,2012,16(5):843-846.
[11]安梦培,张守元,张淹,等.阿胶对免疫低下模型小鼠免疫功能的影响[J].药物评价研究,2018,41(4):567-571.
[12]Bellissimo F,Pinzone M R,Celesia B M,et al.Baseline CD4/CD8 T-cell ratio predicts prompt immune restoration upon c ART initiation[J].Curr HIV Res,2016,14(6):491-496.
[13]朱孝娟,孙玉薇,宋柏捷.大豆皂苷对荷瘤小鼠化疗疗效及T淋巴细胞亚群的影响[J].实用预防医学,2015,22(3):292-295.
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