高效液相色谱法检测鸡肉中的泰乐菌素

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英文篇名：Determination of tylosin residue in chicken using high performance liquid chromatograph 作者：罗玉莹 ; 王艳 ; 李艳萍 英文作者：LUO Yuying;WANG Yan;LI Yanping;School of Petrochemical Engineering,Yinchuan University; 关键词：高效液相色谱 ; 泰乐菌素 ; 鸡肉 英文关键词：high performance liquid chromatography;;tylosin;;chicken 中文刊名：NXSH 英文刊名：Petrochemical Industry Application 机构：银川大学石油化工学院; 出版日期：2018-06-25 出版单位：石油化工应用 年：2018 期：v.37;No.199 基金：宁夏回族自治区创新创业项目,项目编号:NXCX2016389 语种：中文; 页：NXSH201806029 页数：4 CN：06 ISSN：64-1058/TE 分类号：133-136

摘要

建立了鸡肉中泰乐菌素的高效液相色谱检测方法。样品经甲醇提取,正己烷脱脂。流动相为甲醇:磷酸二氢钾(0.04 mol/m L,p H 2.5)溶液,流速为1 m L/min,等度洗脱。泰乐菌素浓度在2μg/m L~16μg/m L范围内呈良好线性关系,相关系数R2=0.999 7。在2.0μg/m L,3.0μg/m L,4.0μg/m L3个添加水平下,回收率范围为96.33%~101.73%,精密度为4.41%。该方法灵敏,简单,快速且准确,适用于鸡肉样品中泰乐菌素残留量的检测。
        An efficient liquid chromatography was developed to test tylosin in chicken. The sample was extracted with methanol and degreased with hexane. The mobile phase was methanol-0.04 mol/m L monopotassium phosphate and the flow rate was 1 m L/min with isocratic elution. The tylosin concentrations within the range of 2 μg/m L~16 μg/m L exhibited a better linearity, with the coefficient of association R~2=0.999 7. At different addition levels(2.0 μg/m L, 3.0 μg/m L and 4.0 μg/m L), the range of recovery was 96.33 %~101.73 % and the precision reached 4.41 %. The liquid chromatography is a sensitive and quick method of easy operation and precise results. The method is suitable for the detection of tylosin residue in chicken.

引文

[1]赵东豪,贺利民,聂建荣,等.HPLC-MS-MS检测猪肉中六种大环内酯类抗生素[J].分析实验室,2009,28(1):117-119.
    [2]崔阳,王冰,徐刚,等.超高效液相色谱-串联质谱法检测猪血清中泰乐菌素残留的建立[J].东北农业大学学报,2011,42(9):125-130.
    [3]SCHEER M.Studies on the antimicrobial agents against five pathogens isolated in calves in the Netherlands[J].Vet Quart,1990,12(4):212-219.
    [4]陆孙琴,李轶,黄晶晶,等.污水处理厂二级出水中总异养菌群对6种抗生素的耐受性研究[J].环境科学,2011,11(32):3419-3424.
    [5]Sanbonsuge A,Takease T,Shiho D,et al.Gas chromatographymass spectrometric determination of ivermectin following trim-ethysilylation with application to residue analysis in biological meat tissue samples[J].Analytical Methods,2011,(3):2016-2064.
    [6]王凤美,陈军辉,林黎明,等.UPLC-MS/MS法对动物源性食品中12种大环内酯类抗生素残留的测定[J].分析测试学报,2009,28(7):784-788.
    [7]SAMANIDOU V F,NIKOLAIDOU K I,PAPADOYANNIS I N.Development and validation of an HPLC confirma-tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC[J].Journal of Separation Science,2005,28(17):2247-2258.
    [8]严丽娟,张峰,方恩华,等.超高效液相色谱-电喷雾串联质谱法测定动物饲料中的大环内酯类抗生素和林可胺类抗生素[J].色谱,2010,28(11):1038-1042.