脂联素基因+45和+276多态性与川崎病的关系
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摘要
目的检测川崎病患儿脂联素基因+45T/G、+276G/T多态性分布情况,探讨其与川崎病发病和冠状动脉损伤(CAL)发生的关系。方法采取病例对照研究方法,选取81例川崎病患儿(其中11例并发CAL)和100例正常体检儿童(对照组)。采用基因测序方法测定脂联素基因+45和+276位点的多态性分布。结果川崎病组脂联素基因+45位点基因型TT、TG、GG以及T/G的等位基因频率与对照组比较差异无统计学意义(P>0.05);川崎病组中并发CAL者与无CAL者该位点的基因型分布频率和等位基因频率比较差异亦无统计学意义(P>0.05)。川崎病组与对照组脂联素基因+276位点基因型GG、GT、TT及G/T等位基因频率的比较差异均有统计学意义(P<0.05);GG基因型为川崎病发病的危险因素(OR=2.313,P=0.006)。川崎病患儿中CAL组脂联素基因+276位点基因型分布与非CAL组比较差异无统计学意义(P>0.05)。结论脂联素基因+276位点多态性可能与川崎病的发生相关,但与CAL的发生无关;脂联素基因+45位点多态性可能与川崎病和CAL的发生均无关。
Objective To investigate the distribution of adiponectin +45 T/G and +276 G/T polymorphisms and its association with the development of Kawasaki disease and coronary artery lesion(CAL). Methods A total of 81 children with Kawasaki disease(among whom 11 had CAL) and 100 normal children who underwent physical examination(control group) were enrolled in a case-control study. Sequencing was performed to investigate the distribution of adiponectin +45 T/G and +276 G/T polymorphisms. Results There were no significant differences between the Kawasaki disease and control groups in the frequencies of TT, TG, and GG genotypes and T/G alleles of +45 T/G polymorphism in the adiponectin gene(P>0.05). In the Kawasaki disease group, there were also no significant differences in the genotype and allele frequencies of the +45 T/G polymorphism between the children with CAL and those without(P>0.05). There were significant differences between the Kawasaki disease and control groups in the frequencies of GG, GT, and TT genotypes and G/T alleles of +276 G/T polymorphism in the adiponectin gene(P<0.05). GG genotype was a risk factor for the development of Kawasaki disease(OR=2.313, P=0.006). In the Kawasaki disease group, there was no significant difference in the genotype distribution of the +276 G/T polymorphism between the children with CAL and those without(P>0.05). Conclusions The adiponectin +276 G/T polymorphism may be associated with the development of Kawasaki disease, but not associated with CAL. The adiponectin +45 T/G polymorphism may not be associated with Kawasaki disease or CAL.
Objective To investigate the distribution of adiponectin +45 T/G and +276 G/T polymorphisms and its association with the development of Kawasaki disease and coronary artery lesion(CAL). Methods A total of 81 children with Kawasaki disease(among whom 11 had CAL) and 100 normal children who underwent physical examination(control group) were enrolled in a case-control study. Sequencing was performed to investigate the distribution of adiponectin +45 T/G and +276 G/T polymorphisms. Results There were no significant differences between the Kawasaki disease and control groups in the frequencies of TT, TG, and GG genotypes and T/G alleles of +45 T/G polymorphism in the adiponectin gene(P>0.05). In the Kawasaki disease group, there were also no significant differences in the genotype and allele frequencies of the +45 T/G polymorphism between the children with CAL and those without(P>0.05). There were significant differences between the Kawasaki disease and control groups in the frequencies of GG, GT, and TT genotypes and G/T alleles of +276 G/T polymorphism in the adiponectin gene(P<0.05). GG genotype was a risk factor for the development of Kawasaki disease(OR=2.313, P=0.006). In the Kawasaki disease group, there was no significant difference in the genotype distribution of the +276 G/T polymorphism between the children with CAL and those without(P>0.05). Conclusions The adiponectin +276 G/T polymorphism may be associated with the development of Kawasaki disease, but not associated with CAL. The adiponectin +45 T/G polymorphism may not be associated with Kawasaki disease or CAL.
引文
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[13]曾婷,施丹,顾科峰,等.类风湿性关节炎患者脂联素水平与动脉粥样硬化的相关性[J].中华老年心脑血管病杂志,2016,18(2):161-164.
[14]Zhan D,Thumtecho S,Tanavalee A,et al.Association of adiponectin gene polymorphisms with knee osteoarthritis[J].World J Orthop,2017,8(9):719-725.
[15]Rodríguez-Rodríguez L,García-Bermúdez M,González-Juanatey C,et al.Lack of association between ADIPOQ rs266729 and ADIPOQ rs1501299 polymorphisms and cardiovascular disease in rheumatoid arthritis patients[J].Tissue Antigens,2011,77(1):74-78.
[16]Daniels LB,Tjajadi MS,Walford HH,et al.Prevalence of Kawasaki disease in young adults with suspected myocardial ischemia[J].Circulation,2012,125(20):2447-2453.
[17]Daniels LB,Gordon JB,Burns JC.Kawasaki disease:late cardiovascular sequelae[J].Curr Opin Cardiol,2012,27(6):572-577.
[18]Zhang BC,Li WM,Xu YW.A meta-analysis of the association of adiponectin gene polymorphisms with coronary heart disease in Chinese Han population[J].Clin Endocrinol(Oxf),2012,76(3):358-364.
[19]Cheung CY,Hui EY,Cheung BM,et al.Adiponectin gene variants and the risk of coronary heart disease:a 16-year longitudinal study[J].Eur J Endocrinol,2014,171(1):107-115.
[20]Hou H,Ge S,Zhao L,et al.An updated systematic review and meta-analysis of association between adiponectin gene polymorphisms and coronary artery disease[J].OMICS,2017,21(6):340-351.
[2]Villarreal-Molina MT,Antuna-Puente B.Adiponectin:antiinflammatory and cardioprotective effects[J].Biochimie,2012,94(10):2143-2149.
[3]张闽,彭瑜,吕树铮.脂联素基因多态性与早发心肌梗死的相关性研究[J].中华心血管病杂志,2016,44(7):577-582.
[4]黄秒,董国庆,蒋红英,等.川崎病患儿血清脂联素水平的变化[J].中国当代儿科杂志,2015,17(1):35-39.
[5]杜忠东,梁翊常.皮肤黏膜淋巴结综合征[M]//胡亚美,江载芳.诸福棠实用儿科学.第7版.北京:人民卫生出版社,2002:778-788.
[6]Denby KJ,Clark DE,Markham LW.Management of Kawasaki disease in adults[J].Heart,2017,103(22):1760-1769.
[7]Uehara R,Belay ED.Epidemiology of Kawasaki disease in Asia,Europe,and the United States[J].J Epidemiol,2012,22(2):79-85.
[8]Onouchi Y.The genetics of Kawasaki disease[J].Int J Rheum Dis,2017,21(1):26-30.
[9]Kim HJ,Choi EH,Kil HR.Association between adipokines and coronary artery lesions in children with Kawasaki disease[J].J Korean Med Sci,2014,29(10):1385-1390.
[10]Gupta AC,Misra R,Sakhuja P,et al.Association of adiponectin gene functional polymorphisms(-11377C/G and+45T/G)with nonalcoholic fatty liver disease[J].Gene,2012,496(1):63-67.
[11]Peters KE,Beilby J,Cadby G,et al.A comprehensive investigation of variants in genes encoding adiponectin(ADIPOQ)and its receptors(ADIPOR1/R2),and their association with serum adiponectin,type 2 diabetes,insulin resistance and the metabolic syndrome[J].BMC Med Genet,2013,14:15.
[12]Maintinguer Norde M,Okié,de Castro IA,et al.Influence of adiponectin gene variants and plasma fatty acids on systemic inflammation state association-A cross-sectional population-based study,S?o Paulo,Brazil[J].Mol Nutr Food Res,2016,60(2):278-286.
[13]曾婷,施丹,顾科峰,等.类风湿性关节炎患者脂联素水平与动脉粥样硬化的相关性[J].中华老年心脑血管病杂志,2016,18(2):161-164.
[14]Zhan D,Thumtecho S,Tanavalee A,et al.Association of adiponectin gene polymorphisms with knee osteoarthritis[J].World J Orthop,2017,8(9):719-725.
[15]Rodríguez-Rodríguez L,García-Bermúdez M,González-Juanatey C,et al.Lack of association between ADIPOQ rs266729 and ADIPOQ rs1501299 polymorphisms and cardiovascular disease in rheumatoid arthritis patients[J].Tissue Antigens,2011,77(1):74-78.
[16]Daniels LB,Tjajadi MS,Walford HH,et al.Prevalence of Kawasaki disease in young adults with suspected myocardial ischemia[J].Circulation,2012,125(20):2447-2453.
[17]Daniels LB,Gordon JB,Burns JC.Kawasaki disease:late cardiovascular sequelae[J].Curr Opin Cardiol,2012,27(6):572-577.
[18]Zhang BC,Li WM,Xu YW.A meta-analysis of the association of adiponectin gene polymorphisms with coronary heart disease in Chinese Han population[J].Clin Endocrinol(Oxf),2012,76(3):358-364.
[19]Cheung CY,Hui EY,Cheung BM,et al.Adiponectin gene variants and the risk of coronary heart disease:a 16-year longitudinal study[J].Eur J Endocrinol,2014,171(1):107-115.
[20]Hou H,Ge S,Zhao L,et al.An updated systematic review and meta-analysis of association between adiponectin gene polymorphisms and coronary artery disease[J].OMICS,2017,21(6):340-351.
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