miR-574-5p在子痫前期中的表达研究
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摘要
目的探讨miR-574-5p在子痫前期患者血浆中的表达水平及其与胎盘组织中表达量的关系。方法收集2014年2月至2015年12月在深圳市罗湖区人民医院住院的子痫前期和同期住院正常晚期妊娠孕妇各20例,应用Real Time PCR法检测血浆和胎盘组织miR-574-5p的表达水平。结果子痫前期患者的血浆和胎盘组织中miR-574-5p表达量较正常对照组均明显升高。与对照组(1.01±0.03)相比,子痫前期组的血浆中miR-574-5p的表达量(3.80±0.19)显著升高(P<0.01);子痫前期组的胎盘组织miR-574-5p水平(4.15±0.18)明显高于正常对照组(1.13±0.10)(P<0.01);且随着子痫前期血浆中miR-574-5p表达量的增加,胎盘组织中miR-574-5p表达量也呈现增高趋势,二者具有明显的相关性(r=0.843)。结论 miR-574-5p有望成为子痫前期筛查的生物标志物。
Objective To investigate the expression level of miR-574-5 p in the plasma of the patients with preeclampsia,and to explore its relationship with that in placentas.Methods Twenty patients with preeclampsia and 20 normal pregnant women(the control group)were selected,who were hospitalized in the People's Hospital of Luohu District of Shenzhen city from February 2014 to December 2015.The expression of miR-574-5 p in the plasma and placentas were measured by real-time PCR.Results The expression of miR574-5 p in the plasma and placenta of preelampsia patients was increased singnificantly,compared with the control group.The expression of miR-574-5 p in the preeclampsia plasma was significantly higher than that in the control group(3.80±0.19 vs. 1.01±0.03),and the differences were statistically significant(P<0.01).The expression of miR-574-5 p in the preeclampsia placenta tissue was higher than that in the control group(4.15±0.18 vs. 1.13±0.10),the differences being statistically significant(P<0.01).With the increase of miR-574-5 p in the preeclampsia plasma,the expression of miR-574-5 p in the placenta tissue also increased. The expression of placenta miR-574-5 p was positively correlated with plasma miR-574-5 p(r=0.843).Conclusion The miR-574-5 p may become a biomarker to diagnose preeclampsia.
Objective To investigate the expression level of miR-574-5 p in the plasma of the patients with preeclampsia,and to explore its relationship with that in placentas.Methods Twenty patients with preeclampsia and 20 normal pregnant women(the control group)were selected,who were hospitalized in the People's Hospital of Luohu District of Shenzhen city from February 2014 to December 2015.The expression of miR-574-5 p in the plasma and placentas were measured by real-time PCR.Results The expression of miR574-5 p in the plasma and placenta of preelampsia patients was increased singnificantly,compared with the control group.The expression of miR-574-5 p in the preeclampsia plasma was significantly higher than that in the control group(3.80±0.19 vs. 1.01±0.03),and the differences were statistically significant(P<0.01).The expression of miR-574-5 p in the preeclampsia placenta tissue was higher than that in the control group(4.15±0.18 vs. 1.13±0.10),the differences being statistically significant(P<0.01).With the increase of miR-574-5 p in the preeclampsia plasma,the expression of miR-574-5 p in the placenta tissue also increased. The expression of placenta miR-574-5 p was positively correlated with plasma miR-574-5 p(r=0.843).Conclusion The miR-574-5 p may become a biomarker to diagnose preeclampsia.
引文
[1]杨孜.多因素、多通路、多机制致病解子痫前期综合征制胜真实世界临床实践[J].中国实用妇科与产科杂志,2017,33(1):45-51.
[2]Chen DB,Wang W.Human placental micro RNAs and preeclampsia[J].Biol Reprod,2013,88(5):130.
[3]Foss KM,Sima C,Ugolini D,et al.Mi R-1254 and mi R-574-5p:serum-based micro RNA biomarkers for early-stage non-small cell lung cancer[J].J Thorac Oncol,2011,6:482-488.
[4]Chim SS,Shing TK,Hung EC,et al.Detection and characterization of placental micro RNA in matemal plasma[J].Clin Chem,2008,54(3):482-490.
[5]Al-Kafaji G,Al-Mahroos G,Al-Muhtaresh HA,et al.Circulating endothelium-enriched microrna-126 as a potential biomarker for coronary artery disease in type 2 diabetes mellitus patients[J].Biomarkers,2016,20:1-40.
[6]卢健翔,石瑾秋,周芸,等.mi R-155对子痫前期滋养层细胞炎症应答的调控研究[J].中国实用妇科与产科杂志,2017,33(7):742-745.
[7]Zhang WC,Chin TM,Yang H,et al.Tumour-initiating cell-specific mir-1246 and mir-1290 expression converge to promote non-small cell lung cancer progression[J].Nat Commun,2016,7:11702.
[8]Zhou J,Shao G,Chen X,et al.mi RNA 206 and mi RNA 574-5p are highly expressed in coronary artery disease[J].Biosci Rep,2015,36(1):e00295.
[9]Zhou R,Zhou X,Yin Z,et al.Micro RNA-574-5p promotes metastasis of non-small cell lung cancer by targeting PTPRU[J].Sci Rep,2016,6:35714.
[10]Li Q,Li X,Guo Z,et al.Micro RNA-574-5p was pivotal for TLR9 signaling enhanced tumor progression via down-regulating checkpoint suppressor 1 in human lung cancer[J].Plo S One,2012,7(11):e48278.
[11]Ji S,Ye G,Zhang J,et al.Mi R-574-5p negatively regulates Qki6/7 to impactβ-catenin/Wnt signaling and the development of colorectal cancer[J].Gut,2013,62(5):716-726.
[12]Armstrong RA,Reynolds RM,Leask R,et al.Decreased serum levels of kisspeptin in early pregnancy are associated with intrauterine growth restriction and preeclampsia[J].Prenat Diagn,2009,29(10):982-985.
[13]Gonen R,Shahar R,Grimpel YI,et al.Placental protein 13 as an early marker for pre-eclampsia:a prospective longitudinal study[J].BJOG,2008,115(12):1465-1472.
[2]Chen DB,Wang W.Human placental micro RNAs and preeclampsia[J].Biol Reprod,2013,88(5):130.
[3]Foss KM,Sima C,Ugolini D,et al.Mi R-1254 and mi R-574-5p:serum-based micro RNA biomarkers for early-stage non-small cell lung cancer[J].J Thorac Oncol,2011,6:482-488.
[4]Chim SS,Shing TK,Hung EC,et al.Detection and characterization of placental micro RNA in matemal plasma[J].Clin Chem,2008,54(3):482-490.
[5]Al-Kafaji G,Al-Mahroos G,Al-Muhtaresh HA,et al.Circulating endothelium-enriched microrna-126 as a potential biomarker for coronary artery disease in type 2 diabetes mellitus patients[J].Biomarkers,2016,20:1-40.
[6]卢健翔,石瑾秋,周芸,等.mi R-155对子痫前期滋养层细胞炎症应答的调控研究[J].中国实用妇科与产科杂志,2017,33(7):742-745.
[7]Zhang WC,Chin TM,Yang H,et al.Tumour-initiating cell-specific mir-1246 and mir-1290 expression converge to promote non-small cell lung cancer progression[J].Nat Commun,2016,7:11702.
[8]Zhou J,Shao G,Chen X,et al.mi RNA 206 and mi RNA 574-5p are highly expressed in coronary artery disease[J].Biosci Rep,2015,36(1):e00295.
[9]Zhou R,Zhou X,Yin Z,et al.Micro RNA-574-5p promotes metastasis of non-small cell lung cancer by targeting PTPRU[J].Sci Rep,2016,6:35714.
[10]Li Q,Li X,Guo Z,et al.Micro RNA-574-5p was pivotal for TLR9 signaling enhanced tumor progression via down-regulating checkpoint suppressor 1 in human lung cancer[J].Plo S One,2012,7(11):e48278.
[11]Ji S,Ye G,Zhang J,et al.Mi R-574-5p negatively regulates Qki6/7 to impactβ-catenin/Wnt signaling and the development of colorectal cancer[J].Gut,2013,62(5):716-726.
[12]Armstrong RA,Reynolds RM,Leask R,et al.Decreased serum levels of kisspeptin in early pregnancy are associated with intrauterine growth restriction and preeclampsia[J].Prenat Diagn,2009,29(10):982-985.
[13]Gonen R,Shahar R,Grimpel YI,et al.Placental protein 13 as an early marker for pre-eclampsia:a prospective longitudinal study[J].BJOG,2008,115(12):1465-1472.