回回药方圣世散对先兆流产小鼠母胎界面SOCS1、SOCS3表达的影响
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摘要
目的:通过观察回回药方圣世散对先兆流产模型小鼠母胎界面SOCS1、SOCS3蛋白表达的影响,从免疫学角度探讨圣世散的保胎机制。方法:用CBA/J×BALB/C小鼠建立正常妊娠组,CBA/J×DBA/2小鼠建立先兆流产模型对照组及圣世散高、中、低剂量组,免疫组化法检测母胎界面SOCS1、SOCS3蛋白表达。结果:与正常妊娠组比较,先兆流产模型组SOCS1升高,SOCS3降低;与先兆流产模型组比较,圣世散高、中剂量组SOCS1降低,SOCS3升高。结论:圣世散可能通过降低先兆流产小鼠母胎界面SOCS1表达,升高SOCS3表达,调节Th1/Th2的平衡,发挥保胎作用。
Objective: Through the observation of Hui prescription Shenshi Powder on the matermal-fetal interface SOCS1 and SOCS3 protein expression of threatened abortion mice model. Inquire the immune mechanism of Shengshi Powder on protecting the fetus. Methods: The normal pregnancy group was established by mating CBA/J × BALB/c mice.The model group of threatened abortion and the high,medium,low dose groups of Shengshi Powder were established by mating CBA/J × DBA/2 mice. To observe the expression of SOCS1 and SOCS3 in the maternal-fetal interface by immunohistochemical method. Result: Compared with normal pergnancy mice,the SOCS1 expression of threatened abortion group had increased and the SOCS3 had decreased; Compared with the model group of threatened abortion,Both high and middle group of Shengshi powder could decrease SOCS1 and increase SOCS3. Conclusion: Shengshi Powder may reduce the expression of SOCS1 and rise SOCS3. It may regulate the imbalance of Th1/Th2 to crue Threaened abortion.
Objective: Through the observation of Hui prescription Shenshi Powder on the matermal-fetal interface SOCS1 and SOCS3 protein expression of threatened abortion mice model. Inquire the immune mechanism of Shengshi Powder on protecting the fetus. Methods: The normal pregnancy group was established by mating CBA/J × BALB/c mice.The model group of threatened abortion and the high,medium,low dose groups of Shengshi Powder were established by mating CBA/J × DBA/2 mice. To observe the expression of SOCS1 and SOCS3 in the maternal-fetal interface by immunohistochemical method. Result: Compared with normal pergnancy mice,the SOCS1 expression of threatened abortion group had increased and the SOCS3 had decreased; Compared with the model group of threatened abortion,Both high and middle group of Shengshi powder could decrease SOCS1 and increase SOCS3. Conclusion: Shengshi Powder may reduce the expression of SOCS1 and rise SOCS3. It may regulate the imbalance of Th1/Th2 to crue Threaened abortion.
引文
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[3]LINDEMANN C,HACKMANN O,DELIC S,et al.SOCS3promoter methylation is mutually exclusive to EGFR amplification in gliomas and promotes glioma cell invasion through STAT3 and FAK activation[J].Acta Neuropathol,2011,122(2):241-251.
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[5]杜小利,吴心芳,罗文玉,等.回回药方圣世散对早期先兆流产患者血人绒毛膜促性腺激素、雌激素及孕激素的影响[J].四川中医,2013,31(11):63-64.
[6]苏艳姣,刘宏奇.中医药治疗Th1/Th2细胞因子失衡致自然流产的机理研究[J].中国中医药现代远程教育,2016,2(14):146-148.
[7]王若光,尤昭玲,刘小丽,等.黄芪丹参复方成分对妊高征模型孕鼠胎盘滋养细胞内皮型一氧化氮合酶及血管内皮生长因子mRNA表达影响研究[J].中国中医药信息杂志,2005,12(2):44-47.
[8]周雯慧,杜美蓉,李大金,等.环素A对人蜕膜免疫细胞分泌IL-10与IFN-γ的调控作用[J].现代免疫学,2006,26(4):314-317.
[9]孙燕,陈佩.TNF-á和IL-6与复发性流产的相关性研究[J].中国妇幼保健,2012,25(27):3940-3943.
[10]KUBO M,HANADA T,YOSHIMURA A.Suppressors of cytokine signaling and immunity[J].Nature Immun,2003,41(3):1169-1176.
[11]KREBS D L,HILTON D J.SOCS physiological suppressors of cytokine signaling[J].Sci,2000,113(16):2813-2819.
[12]SEKI Y H,INOUE N,NAGATA N,et al.SOCS-3 regulates onset and maintenance of Th2 mediated allergic responses[J].Nat Med,2003,9(8):1047-1054.
[13]徐晓琴,徐向荣.补肾中药对卵巢早衰患者卵巢储备功能及免疫调节影响的研究[J].中华全科医学,2014,12(12):2004-2006.
[14]杜小利,朱宁娃.回回药方圣世散治疗先兆流产35例[J].中医杂志,2012,53(3):247.
[15]林羿,曾耀英.反复自然流产小鼠妊振免疫耐受机理研究[J].中华妇产科杂志,2002,37(2):122-123.
[16]赵爱民,林其德.Th1和Th2型细胞因子与复发性流产的相关性[J].上海第二医科大学学报,2000,20(S1):16-18.
[2]尤昭玲,赖毛华.何冬梅,等.正常妊娠与复发性流产小鼠母界面SOCS1、SOCS2和SOCS3mRNA表达的差异[J].中国中医药科技,2008,15(6):4531-4535.
[3]LINDEMANN C,HACKMANN O,DELIC S,et al.SOCS3promoter methylation is mutually exclusive to EGFR amplification in gliomas and promotes glioma cell invasion through STAT3 and FAK activation[J].Acta Neuropathol,2011,122(2):241-251.
[4]高如宏,陈卫川.回族医方集粹[M].宁夏:人民出版社,2008:213-214.
[5]杜小利,吴心芳,罗文玉,等.回回药方圣世散对早期先兆流产患者血人绒毛膜促性腺激素、雌激素及孕激素的影响[J].四川中医,2013,31(11):63-64.
[6]苏艳姣,刘宏奇.中医药治疗Th1/Th2细胞因子失衡致自然流产的机理研究[J].中国中医药现代远程教育,2016,2(14):146-148.
[7]王若光,尤昭玲,刘小丽,等.黄芪丹参复方成分对妊高征模型孕鼠胎盘滋养细胞内皮型一氧化氮合酶及血管内皮生长因子mRNA表达影响研究[J].中国中医药信息杂志,2005,12(2):44-47.
[8]周雯慧,杜美蓉,李大金,等.环素A对人蜕膜免疫细胞分泌IL-10与IFN-γ的调控作用[J].现代免疫学,2006,26(4):314-317.
[9]孙燕,陈佩.TNF-á和IL-6与复发性流产的相关性研究[J].中国妇幼保健,2012,25(27):3940-3943.
[10]KUBO M,HANADA T,YOSHIMURA A.Suppressors of cytokine signaling and immunity[J].Nature Immun,2003,41(3):1169-1176.
[11]KREBS D L,HILTON D J.SOCS physiological suppressors of cytokine signaling[J].Sci,2000,113(16):2813-2819.
[12]SEKI Y H,INOUE N,NAGATA N,et al.SOCS-3 regulates onset and maintenance of Th2 mediated allergic responses[J].Nat Med,2003,9(8):1047-1054.
[13]徐晓琴,徐向荣.补肾中药对卵巢早衰患者卵巢储备功能及免疫调节影响的研究[J].中华全科医学,2014,12(12):2004-2006.
[14]杜小利,朱宁娃.回回药方圣世散治疗先兆流产35例[J].中医杂志,2012,53(3):247.
[15]林羿,曾耀英.反复自然流产小鼠妊振免疫耐受机理研究[J].中华妇产科杂志,2002,37(2):122-123.
[16]赵爱民,林其德.Th1和Th2型细胞因子与复发性流产的相关性[J].上海第二医科大学学报,2000,20(S1):16-18.
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