棉花Bax inhibitor-1影响内质网胁迫介导的细胞死亡的研究
|
摘要
Bax inhibitor-1(BI-1)是调控内质网胁迫(endoplasmic reticulum stress,ER stress)介导的细胞死亡的关键因子,在植物耐逆中具有重要作用。目前,棉花BI-1(GhBI-1)的耐逆功能及其调控细胞死亡的相关报道较少。在本研究中,采用ER stress专一性诱导毒素——衣霉素(tunicamycin,TM)对棉花幼苗进行胁迫处理,实时定量PCR结果表明,GhBI-1的TM诱导表达具有组织特异性,根GhBI-1对TM的响应更加强烈。通过TM抗性试验及细胞死亡的分析发现,GhBI-1的表达提高了拟南芥的TM抗性,减缓了ER stress介导的细胞死亡。此外,未折叠蛋白反应信号通路中的bZip60转录因子基因的表达受GhBI-1的调控。
Bax inhibitor-1(BI-1) regulates cell death mediated by endoplasmic reticulum stress(ER stress),and plays important roles in plant stress tolerance. Up to now,the role of cotton BI-1(GhBI-1)involved in stress tolerance and cell death remain largely unknown. In the present study,we characterized the expression patterns of GhBI-1 under ER stress conditions induced by tunicamycin(TM). The results of real-time quantitative PCRs showed that the inducible expression pattern of GhBI-1 was tissue specific. The expression of GhBI-1 in roots is stronger compared with in leaves under ER stress conditions. The overexpression of GhBI-1 increased the TM resistance of transgenic Arabidopsis and delayed cell death mediated by ER stress. The analysis of the expression of b Zip60 involved in unfolded protein response showed that GhBI-1 activated the avtivity of b Zip60 and improved cell tolerance to ER stress.
Bax inhibitor-1(BI-1) regulates cell death mediated by endoplasmic reticulum stress(ER stress),and plays important roles in plant stress tolerance. Up to now,the role of cotton BI-1(GhBI-1)involved in stress tolerance and cell death remain largely unknown. In the present study,we characterized the expression patterns of GhBI-1 under ER stress conditions induced by tunicamycin(TM). The results of real-time quantitative PCRs showed that the inducible expression pattern of GhBI-1 was tissue specific. The expression of GhBI-1 in roots is stronger compared with in leaves under ER stress conditions. The overexpression of GhBI-1 increased the TM resistance of transgenic Arabidopsis and delayed cell death mediated by ER stress. The analysis of the expression of b Zip60 involved in unfolded protein response showed that GhBI-1 activated the avtivity of b Zip60 and improved cell tolerance to ER stress.
引文
[1]Welihinda A A,Tirasophon W,Kaufman R J.The cellular response to protein misfolding in the endoplasmic reticulum[J].Gene Expr.,1999,7:293-300.
[2]Iwata Y,Fedoroff N V,Koizumi N.Arabidopsis b ZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response[J].Plant Cell,2008,20:3107-3121.
[3]Watanabe N,Lam E.BAX inhibitor-1 modulates endoplasmic reticulum stress-mediated programmed cell death in Arabidopsis[J].J.Biol.Chem.,2008,283:3200-3210.
[4]Ruberti C,Kim S J,Stefano G,et al.Unfolded protein response in plants:one master,many questions[J].Curr.Opin.Plant Biol.,2015,27:59-66.
[5]Lam E.Controlled cell death,plant survival and development[J].Nat.Rev.Mol.Cell Biol.,2004,5:305-315.
[6]Watanabe N,Lam E.Bax Inhibitor-1,a conserved cell death suppressor,is a key molecular switch downstream from a variety of biotic and abiotic stress signals in plants[J].Int.J.Mol.Sci.,2009,10:3149-3167.
[7]Xu Q,Reed J C.Bax inhibitor-1,a mammalian apoptosis suppressor identified by functional screening in yeast[J].Mol.Cell,1998,1:337-346.
[8]Bultynck G,Kiviluoto S,Henke N,et al.The C terminus of Bax inhibitor-1 forms a Ca2+-permeable channel pore[J].J.Biol.Chem.,2012,287:2544-2557.
[9]Hunsberger J G,Machado-Vieira R,Austin D R,et al.Bax inhibitor 1,a modulator of calcium homeostasis,confers affective resilience[J].Brain Res.,2011,1403:19-27.
[10]Li B,Yadav R K,Jeong G S,et al.The characteristics of Bax inhibitor-1 and its related diseases[J].Curr.Mol.Med.,2014,14:603-615.
[11]Eichmann R,Bischof M,Weis C,et al.BAX INHIBITOR-1is required for full susceptibility of barley to powdery mildew[J].Mol.Plant Microbe Interact.,2010,23:1217-1227.
[12]Isbat M,Zeba N,Kim S R,et al.A BAX inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transgenic tobacco[J].J.Plant Physiol.,2009,166:1685-1693.
[13]Ishikawa T,Takahara K,Hirabayashi T,et al.Metabolome analysis of response to oxidative stress in rice suspension cells overexpressing cell death suppressor Bax inhibitor-1[J],Plant Cell Physiol.,2010,51:9-20.
[14]Kawai-Yamada M,Jin L,Yoshinaga K,et al.Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1(At BI-1)[J].Proc.Natl.Acad.Sci.USA,2001,98:12295-12300.
[15]Bolduc N,Ouellet M,Pitre F,et al.Molecular characterization of two plant BI-1 homologues which suppress Bax-induced apoptosis in human 293 cells[J].Planta,2003,216:377-386.
[16]霍雪寒,张景霞,高阳,等.陆地棉Gh BI-1基因在拟南芥中的异位表达及耐盐性研究[J].山东农业科学,2013,45(3):1-3.
[17]郑玲,霍雪寒,王芙蓉,等.陆地棉两个Gh BI-1基因的生物信息学分析[J].山东农业科学,2011(3):4-7.
[18]Koch E,Slusarenko A.Arabidopsis is susceptible to infection by a downy mildew fungus[J].Plant Cell,1990,2:437-445.
[19]Castellani F,Van Rossum B,Diehl A,et al.Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy[J].Nature,2002,420:98-102.
[20]Schroder M,Kaufman R J.ER stress and the unfolded protein response[J].Mutat.Res.,2005,569:29-63.
[21]Ruberti C,Lai Y,Brandizzi F.Recovery from temporary endoplasmic reticulum stress in plants relies on the tissue-specific and largely independent roles of b ZIP28 and b ZIP60,as well as an antagonizing function of BAX-Inhibitor 1 upon the pro-adaptive signaling mediated by b ZIP28[J].Plant J.,2018,93:155-165.
[22]Lu S J,Yang Z T,Sun L,et al.Conservation of IRE1-regulated b ZIP74 mRNA unconventional splicing in rice(Oryza sativa L.)involved in ER stress responses[J].Mol.Plant,2012,5:504-514.
[23]Chi Y H,Melencion S M B,Alinapon C V,et al.The membrane-tethered NAC transcription factor,At NTL7,contributes to ER-stress resistance in Arabidopsis[J].Biochem.Biophys.Res.Commun.,2017,488:641-647.
[24]Angelos E,Ruberti C,Kim S J,et al.Maintaining the factory:the roles of the unfolded protein response in cellular homeostasis in plants[J].Plant J.,2017,90:671-682.
[25]Ruberti C,Brandizzi F.Conserved and plant-unique strategies for overcoming endoplasmic reticulum stress[J].Front Plant Sci.,2014,5:69.
[26]Watanabe N,Lam E.Arabidopsis Bax inhibitor-1:a rheostat for ER stress-induced programmed cell death[J].Plant Signal Behav.,2008,3:564-566.
[27]Yoshida H,Matsui T,Yamamoto A,et al.XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor[J].Cell,2001,107:881-891.
[28]Liu J X,Srivastava R,Che P,et al.An endoplasmic reticulum stress response in Arabidopsis is mediated by proteolytic processing and nuclear relocation of a membrane-associated transcription factor,b ZIP28[J].Plant Cell,2007,19:4111-4119.
[29]Sun L,Lu S J,Zhang S S,et al.The lumen-facing domain is important for the biological function and organelle-to-organelle movement of b ZIP28 during ER stress in Arabidopsis[J].Mol.Plant,2013,6:1605-1615.
[2]Iwata Y,Fedoroff N V,Koizumi N.Arabidopsis b ZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response[J].Plant Cell,2008,20:3107-3121.
[3]Watanabe N,Lam E.BAX inhibitor-1 modulates endoplasmic reticulum stress-mediated programmed cell death in Arabidopsis[J].J.Biol.Chem.,2008,283:3200-3210.
[4]Ruberti C,Kim S J,Stefano G,et al.Unfolded protein response in plants:one master,many questions[J].Curr.Opin.Plant Biol.,2015,27:59-66.
[5]Lam E.Controlled cell death,plant survival and development[J].Nat.Rev.Mol.Cell Biol.,2004,5:305-315.
[6]Watanabe N,Lam E.Bax Inhibitor-1,a conserved cell death suppressor,is a key molecular switch downstream from a variety of biotic and abiotic stress signals in plants[J].Int.J.Mol.Sci.,2009,10:3149-3167.
[7]Xu Q,Reed J C.Bax inhibitor-1,a mammalian apoptosis suppressor identified by functional screening in yeast[J].Mol.Cell,1998,1:337-346.
[8]Bultynck G,Kiviluoto S,Henke N,et al.The C terminus of Bax inhibitor-1 forms a Ca2+-permeable channel pore[J].J.Biol.Chem.,2012,287:2544-2557.
[9]Hunsberger J G,Machado-Vieira R,Austin D R,et al.Bax inhibitor 1,a modulator of calcium homeostasis,confers affective resilience[J].Brain Res.,2011,1403:19-27.
[10]Li B,Yadav R K,Jeong G S,et al.The characteristics of Bax inhibitor-1 and its related diseases[J].Curr.Mol.Med.,2014,14:603-615.
[11]Eichmann R,Bischof M,Weis C,et al.BAX INHIBITOR-1is required for full susceptibility of barley to powdery mildew[J].Mol.Plant Microbe Interact.,2010,23:1217-1227.
[12]Isbat M,Zeba N,Kim S R,et al.A BAX inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transgenic tobacco[J].J.Plant Physiol.,2009,166:1685-1693.
[13]Ishikawa T,Takahara K,Hirabayashi T,et al.Metabolome analysis of response to oxidative stress in rice suspension cells overexpressing cell death suppressor Bax inhibitor-1[J],Plant Cell Physiol.,2010,51:9-20.
[14]Kawai-Yamada M,Jin L,Yoshinaga K,et al.Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1(At BI-1)[J].Proc.Natl.Acad.Sci.USA,2001,98:12295-12300.
[15]Bolduc N,Ouellet M,Pitre F,et al.Molecular characterization of two plant BI-1 homologues which suppress Bax-induced apoptosis in human 293 cells[J].Planta,2003,216:377-386.
[16]霍雪寒,张景霞,高阳,等.陆地棉Gh BI-1基因在拟南芥中的异位表达及耐盐性研究[J].山东农业科学,2013,45(3):1-3.
[17]郑玲,霍雪寒,王芙蓉,等.陆地棉两个Gh BI-1基因的生物信息学分析[J].山东农业科学,2011(3):4-7.
[18]Koch E,Slusarenko A.Arabidopsis is susceptible to infection by a downy mildew fungus[J].Plant Cell,1990,2:437-445.
[19]Castellani F,Van Rossum B,Diehl A,et al.Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy[J].Nature,2002,420:98-102.
[20]Schroder M,Kaufman R J.ER stress and the unfolded protein response[J].Mutat.Res.,2005,569:29-63.
[21]Ruberti C,Lai Y,Brandizzi F.Recovery from temporary endoplasmic reticulum stress in plants relies on the tissue-specific and largely independent roles of b ZIP28 and b ZIP60,as well as an antagonizing function of BAX-Inhibitor 1 upon the pro-adaptive signaling mediated by b ZIP28[J].Plant J.,2018,93:155-165.
[22]Lu S J,Yang Z T,Sun L,et al.Conservation of IRE1-regulated b ZIP74 mRNA unconventional splicing in rice(Oryza sativa L.)involved in ER stress responses[J].Mol.Plant,2012,5:504-514.
[23]Chi Y H,Melencion S M B,Alinapon C V,et al.The membrane-tethered NAC transcription factor,At NTL7,contributes to ER-stress resistance in Arabidopsis[J].Biochem.Biophys.Res.Commun.,2017,488:641-647.
[24]Angelos E,Ruberti C,Kim S J,et al.Maintaining the factory:the roles of the unfolded protein response in cellular homeostasis in plants[J].Plant J.,2017,90:671-682.
[25]Ruberti C,Brandizzi F.Conserved and plant-unique strategies for overcoming endoplasmic reticulum stress[J].Front Plant Sci.,2014,5:69.
[26]Watanabe N,Lam E.Arabidopsis Bax inhibitor-1:a rheostat for ER stress-induced programmed cell death[J].Plant Signal Behav.,2008,3:564-566.
[27]Yoshida H,Matsui T,Yamamoto A,et al.XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor[J].Cell,2001,107:881-891.
[28]Liu J X,Srivastava R,Che P,et al.An endoplasmic reticulum stress response in Arabidopsis is mediated by proteolytic processing and nuclear relocation of a membrane-associated transcription factor,b ZIP28[J].Plant Cell,2007,19:4111-4119.
[29]Sun L,Lu S J,Zhang S S,et al.The lumen-facing domain is important for the biological function and organelle-to-organelle movement of b ZIP28 during ER stress in Arabidopsis[J].Mol.Plant,2013,6:1605-1615.