A~2/O-MBBR反硝化除磷工艺中有机物的迁移转化及利用
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摘要
采用厌氧/缺氧/好氧与移动床生物膜反应器(A~2/O-MBBR)组成的双污泥系统处理实际生活污水,通过投加乙酸钠调节进水碳氮比(C/N),考察系统中有机物的转化利用和代谢途径.试验结果表明:当进水C/N在3.11~6.09范围内波动时,系统均可实现COD的高效去除,最大去除率可达83.85%;但是从VFA和PHAs的转化利用角度,进水C/N不宜超过5.42.此外,碳平衡分析结果也表明,当进水C/N小于5.42时,A~2/O反应器去除的COD百分比最高可达70.10%,MBBR反应器氧化的COD仅占4.32%~5.89%,低C/N条件下碳源的高效利用是促进菌群优化以及提高脱氮除磷效率的关键所在.溶解性碳源组分分布及三维荧光特性的结果显示,尽管系统沿程COD浓度变化不大,但蛋白质和多糖之间存在着转化,且类蛋白物质占主导地位.
A two-sludge system combined anaerobic/anoxic/oxic with moving bed biofilm reactor(A~2/O-MBBR) was used to treat real domestic wastewater. By adding sodium acetate to adjust influent carbon/nitrogen ratio(C/N), the transformation and utilization and metabolic pathways of organic matter in the system were investigated. When the influent C/N ratio varied from 3.11 to 6.09, the system all achieved efficient COD removal with the maximum removal of 83.85%. However, from the perspective of VFA and PHAs transformation, the influent C/N ratio should not exceed 5.42. Additionally, the material balance analysis of carbon also revealed that when the influent C/N ratio was below 5.42, COD removal percentage in the A~2/O reactor was up to 70.10% and that in the MBBR reactor only accounted for 4.32%~5.89%, where the efficient utilization of carbon source was the key to improve microbial community optimization and enhance denitrification and phosphorus removals under the condition of low C/N ratio. The results of dissolved carbon source component distribution and three-dimensional fluorescence properties manifested that proteins and polysaccharides transformed although COD changed gently along the reactor, in which proteins-like substance was the dominant.
A two-sludge system combined anaerobic/anoxic/oxic with moving bed biofilm reactor(A~2/O-MBBR) was used to treat real domestic wastewater. By adding sodium acetate to adjust influent carbon/nitrogen ratio(C/N), the transformation and utilization and metabolic pathways of organic matter in the system were investigated. When the influent C/N ratio varied from 3.11 to 6.09, the system all achieved efficient COD removal with the maximum removal of 83.85%. However, from the perspective of VFA and PHAs transformation, the influent C/N ratio should not exceed 5.42. Additionally, the material balance analysis of carbon also revealed that when the influent C/N ratio was below 5.42, COD removal percentage in the A~2/O reactor was up to 70.10% and that in the MBBR reactor only accounted for 4.32%~5.89%, where the efficient utilization of carbon source was the key to improve microbial community optimization and enhance denitrification and phosphorus removals under the condition of low C/N ratio. The results of dissolved carbon source component distribution and three-dimensional fluorescence properties manifested that proteins and polysaccharides transformed although COD changed gently along the reactor, in which proteins-like substance was the dominant.
引文
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[26]Peng Y,Ge S.Enhanced nutrient removal in three types of step feeding process from municipal wastewater[J].Bioresource Technology,2011,102(11):6405-6413.
[27]Chen Y,Peng C,Wang J,et al.Effect of nitrate recycling ratio on simultaneous biological nutrient removal in a novel anaerobic/anoxic/oxic(A2/O)-biological aerated filter(BAF)system[J].Bioresource Technology,2011,102(10):5722-5727.
[28]Guo J,Sheng F,Guo J,et al.Characterization of the dissolved organic matter in sewage effluent of sequence batch reactor:the impact of carbon source[J].Frontiers of Environmental Science&Engineering in China,2011,6(2):1-8.
[29]崔福义,张兵,唐利.曝气生物滤池技术研究与应用进展[J].环境污染治理技术与设备,2005,6(10):1-7.
[30]Sheng G P,Yu H Q.Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy[J].Water Research,2006,40(6):1233-1239.
[31]Leenheer J A,Jean-Philippe C.Characterizing aquatic dissolved organic matter[J].Environmental Science&Technology,2003,37(1):18-26.
[32]杨毅,杨霞霞.城市污水处理过程中DOM的三维荧光光谱及紫外谱图特性[J].环境工程学报,2015,9(12):5672-5676.
[33]姚萌,罗红元,谢小青,等.城市污水厂活性污泥胞外聚合物的三维荧光特性分析[J].中国环境科学,2012,32(1):94-99.
[2]Wang Z W,Wu Z C.Distribution and transformation of molecular weight of organic matters in membrane bioreactor and conventional activated sludge process[J].Chemical Engineering Journal,2009,150(2):396-402.
[3]Ilani T,Schulz E,Chefetz B.Interactions of organic compounds with wastewater dissolved organic matter:role of hydrophobic fractions[J].Journal of Environmental Quality,2005,34(2):552-562.
[4]Guo J,Peng Y,Guo J,et al.Dissolved organic matter in biologically treated sewage effluent(BTSE):Characteristics and comparison[J].Desalination,2011,278(1-3):365-372.
[5]Wang X,Wang S,Xue T,et al.Treating low carbon/nitrogen(C/N)wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal(SNDPR)systems by strengthening anaerobic intracellular carbon storage[J].Water Research,2015,77:191-200.
[6]Cao G,Wang S,Peng Y,et al.Biological nutrient removal by applying modified four step-feed technology to treat weak wastewater[J].Bioresource Technology,2013,128(1):604-611.
[7]葛士建,彭永臻,曹旭,等.改良UCT分段进水工艺处理生活污水性能优化研究[J].环境科学,2011,32(7):2006-2012.
[8]Wang Y,Peng Y,Stephenson T.Effect of influent nutrient ratios and hydraulic retention time(HRT)on simultaneous phosphorus and nitrogen removal in a two-sludge sequencing batch reactor process[J].Bioresource Technology,2009,100(14):3506-3512.
[9]张淼,何成达,王淑莹,等.A2/O+MBBR系统的快速启动及反硝化除磷特性[J].工程科学与技术,2017,49(2):240-247.
[10]Zhang M,Peng Y,Wang C,et al.Optimization denitrifying phosphorus removal at different hydraulic retention times in a novel anaerobic anoxic oxic-biological contact oxidation process[J].Biochemical Engineering Journal,2016,106:26-36.
[11]张淼,彭永臻,王聪,等.容积分配比对A2/O-生物接触氧化工艺反硝化除磷特性的影响[J].东南大学学报:自然科学版,2015,45(3):531-538.
[12]王聪,王淑莹,张淼,等.硝化液回流比对A2/O-BCO工艺反硝化除磷特性的影响[J].中国环境科学,2014,34(11):2844-2850.
[13]Zhang M,Qing Y,Jianhua Z,et al.Enhancement of denitrifying phosphorus removal and microbial community of long-term operation in an anaerobic anoxic oxic–biological contact oxidation system[J].Journal of Bioscience&Bioengineering,2016,122(4):456-466.
[14]Franson M A H.American public health association American water works association water environment federation[M].Methods,1995.
[15]Oehmen A,Keller-Lehmann B,Zeng RJ,et al.Optimisation of poly-β-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems[J].Journal of Chromatography A,2005,1070(1/2):131-136.
[16]Lowry O H,Rosebrough N J,Farr A L,et al.Protein measurement with the Folin phenol reagent[J].Journal of Biological Chemistry,1951,193(1):265-275.
[17]王聪,王淑莹,张淼,等.厌氧/缺氧/好氧生物接触氧化处理低碳氮比污水的物料平衡[J].农业工程学报,2014,30(19):273-281.
[18]Chuang S H,Ouyang C F.The biomass fractions of heterotrophs and phosphate-accumulating organisms in a nitrogen and phosphorus removal system[J].Water Research,2000,34(8):2283-2290.
[19]王亚宜,王鸿,郭刚,等.厌氧反应时间对反硝化聚磷工艺的影响[J].同济大学学报:自然科学版,2013,41(3):422-427.
[20]Wang X,Peng Y,Wang S,et al.Influence of wastewater composition on nitrogen and phosphorus removal and process control in A2O process[J].Bioprocess&Biosystems Engineering,2006,28(6):397-404.
[21]Chen Y,Peng C,Wang J,et al.Effect of nitrate recycling ratio on simultaneous biological nutrient removal in a novel anaerobic/anoxic/oxic(A2/O)-biological aerated filter(BAF)system[J].Bioresource Technology,2011,102(10):5722-5727.
[22]Wachtmeister A,Kuba T,Loosdrecht M C M V,et al.A sludge characterization assay for aerobic and denitrifying phosphorus removing sludge[J].Water Research,1997,31(3):471-478.
[23]Wei Y,Wang S,Ma B,et al.The effect of poly-β-hydroxyalkanoates degradation rate on nitrous oxide production in a denitrifying phosphorus removal system[J].Bioresource Technology,2014,170(3):175-182.
[24]Chen Y,Li B,Ye L,et al.The combined effects of COD/N ratio and nitrate recycling ratio on nitrogen and phosphorus removal in anaerobic/anoxic/aerobic(A2/O)-biological aerated filter(BAF)systems[J].Biochemical Engineering Journal,2015,93(10):235-242.
[25]葛士建,彭永臻,张亮,等.改良UCT分段进水脱氮除磷工艺性能及物料平衡[J].化工学报,2010,61(4):1009-1017.
[26]Peng Y,Ge S.Enhanced nutrient removal in three types of step feeding process from municipal wastewater[J].Bioresource Technology,2011,102(11):6405-6413.
[27]Chen Y,Peng C,Wang J,et al.Effect of nitrate recycling ratio on simultaneous biological nutrient removal in a novel anaerobic/anoxic/oxic(A2/O)-biological aerated filter(BAF)system[J].Bioresource Technology,2011,102(10):5722-5727.
[28]Guo J,Sheng F,Guo J,et al.Characterization of the dissolved organic matter in sewage effluent of sequence batch reactor:the impact of carbon source[J].Frontiers of Environmental Science&Engineering in China,2011,6(2):1-8.
[29]崔福义,张兵,唐利.曝气生物滤池技术研究与应用进展[J].环境污染治理技术与设备,2005,6(10):1-7.
[30]Sheng G P,Yu H Q.Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy[J].Water Research,2006,40(6):1233-1239.
[31]Leenheer J A,Jean-Philippe C.Characterizing aquatic dissolved organic matter[J].Environmental Science&Technology,2003,37(1):18-26.
[32]杨毅,杨霞霞.城市污水处理过程中DOM的三维荧光光谱及紫外谱图特性[J].环境工程学报,2015,9(12):5672-5676.
[33]姚萌,罗红元,谢小青,等.城市污水厂活性污泥胞外聚合物的三维荧光特性分析[J].中国环境科学,2012,32(1):94-99.
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