基于加速器质谱测量的~(14)C呼气试验样品制备方法研究
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摘要
采用加速器质谱法研究~(14)C呼气试验样品的制备方法。采用长寿命放射性核素~(14)C作为示踪核素,目前已广泛应用于生物代谢、疾病诊断和脏器功能评估。离子源引出的束流强度是衡量加速器质谱灵敏度的重要参数,为了获得较高的束流强度,本工作系统研究了~(14)C呼气试验样品的石墨法、碳酸盐法两种制备方法,拟建立~(14)C呼气试验样品制备流程,确定最高束流强度引出时的最优~(14)C呼气试验样品制备参数,为~(14)C呼气试验广泛应用提供实验基础。
Carbon plays an important role in the metabolism of plants and human.Radiocarbon(~(14)C)is an ideal tracer for vivo human metabolism,disease diagnosis and organ function assessment studies.In this paper,the preparation method of ~(14)C breath test samples for accelerator mass spectrometry(AMS)measurement was developed in China.AMS,with its advantages of high sensitivity,small radioactivity dose,high precision,large measuring range,and long tracer cycle,can be used to measure cosmogenic nuclide ~(14)C,which has long half-life.The current intensity of interested ion in ion source is an important parameter for the sensitivity of AMS measurement.The high beam current can improve the sensitivity of AMS.The preparation methods of breathtest samples of ~(14)C tracer were systematically studied to obtain high beam current using graphitization and carbonatization methods.A reliable preparation procedure of breath test samples for ~(14)C tracer and its optimization parameters were determined by testing beam currents of various samples,which has laid a foundation for ~(14)C-AMS technology at breath test tracer application.
Carbon plays an important role in the metabolism of plants and human.Radiocarbon(~(14)C)is an ideal tracer for vivo human metabolism,disease diagnosis and organ function assessment studies.In this paper,the preparation method of ~(14)C breath test samples for accelerator mass spectrometry(AMS)measurement was developed in China.AMS,with its advantages of high sensitivity,small radioactivity dose,high precision,large measuring range,and long tracer cycle,can be used to measure cosmogenic nuclide ~(14)C,which has long half-life.The current intensity of interested ion in ion source is an important parameter for the sensitivity of AMS measurement.The high beam current can improve the sensitivity of AMS.The preparation methods of breathtest samples of ~(14)C tracer were systematically studied to obtain high beam current using graphitization and carbonatization methods.A reliable preparation procedure of breath test samples for ~(14)C tracer and its optimization parameters were determined by testing beam currents of various samples,which has laid a foundation for ~(14)C-AMS technology at breath test tracer application.
引文
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[14]赵庆章,杨宪林,林德雨,等.基于加速器质谱测量的41 Ca植物示踪样品制备方法研究[J].同位素,2016,29(3):193-197.Zhao Qingzhang,Jiang Pingping,Lin Deyu,et al.Preparation of plant 41 Ca tracer samples for accelerator mass spectrometry[J].Journal of Isotopes,2016,29(4):193-197(in Chinese).
[15]赵墨田,曹永明,陈刚,等.无机质谱概论[M].北京:化学工业出版社,2006:153-164.
[16]杨旭冉,庞义俊,何明,等.用于AMS测量的14 C样品制备方法[J].同位素,2015,28(2):65-68.Yang Xuran,Pang Yijun,He Ming,et al.Method of 14 C sample preparation for AMS measurement[J].Journal of Isotopes,2015,28(2):65-68(in Chinese).
[17]Choe K,Song S,Lee J H,et al.A Study on trapping CO2 by using a molecular sieve for 14 C AMS sample preparation[J].Radiocarbon,2013,55(2-3):421-425.
[18]Xu X,Trumbore S E,Zheng S,et al.Modifying a sealed tube zinc reduction method for preparation of AMS graphite targets:reducing background and attaining high precision[J].Nucl Instrum Meth B,2007,259(1):320-329.
[19]奚娴婷,丁杏芳,付东坡,等.用一年生植物研究大气Δ14 C分布与化石源CO2排放[J].科学通报,2011,56(13):1 026-1 031.
[20]庞义俊.14 C-AMS测量及其在PM2.5源解析中的应用[D].广西师范大学:国家图书馆,2016.
[2]Salehpour M,Possnert G,Bryhni H.Subattomole sensitivity in biological accelerator mass spectrometry[J].Analytical Chemistry,2008,80(10):3 515-3 521.
[3]Vogel J S,Love A H.Quantitating isotopic molecular labels with accelerator mass spectrometry[J].Methods in Enzymology,2005,402:402-422.
[4]Kim S H,Chuang J C,Kelly P B,et al.Carbon isotopes profiles of human whole blood,plasma,red blood cells,urine and feces for biological/biomedical 14 C-accelerator mass spectrometry applications[J].Analytical Chemistry,2011,83(9):3 312-3 318.
[5]Vogel J S,Palmblad N M,Ognibene T,et al.Biochemical paths in humans and cells:Frontiers of AMS bioanalysis[J].Nucl Instrum Meth B,2007,259(1):745-751.
[6]张厚德.呼气试验[M].北京:科学出版社,2007:3-8.
[7]赵思倩,陈宝军,罗志福.CO2呼气试验和H2呼气试验的临床应用[J].同位素,2016,29(3):170-175.Zhao Siqian,Chen Baojun,Luo Zhifu.Clinical applications of CO2and H2breath test[J].Journal of Isotopes,2016,29(3):170-175(in Chinese).
[8]沈承德,黄宝林.14 C年龄测定的液体闪烁计数法[J].地球化学,1980(3):278-281.Shen Chengde,Huang Baolin.Liquid scintillation method for radiocarbon dating[J].Geochimica,1980(3):278-281(in Chinese).
[9]周卫健,周明富,杨礼平.14 C测年系列方法中的小样品14 C液体闪烁计数测年法[J].地球化学,1995(2):146-151.Zhou Weijian,Zhou Mingfu,Yang Liping.Samll sample liquid scintillation counting technique for radiocarbon dating[J].Geochimica,1995(2):146-151(in Chinese).
[10]Bennett C L,Beuken R P,Clover M R,et al.Radiocarbon dating using electrostatic accelerators:negative ion provide the key[J].Science,1977,198:508-510.
[11]Nelson D E,Korteling R G,Stott W R.Carbon14:direct detection at natural concentrations[J].Science,1977,198:507-508.
[12]姜山,董克君,何明.超灵敏加速器质谱技术进展及应用[J].岩矿测试,2012,31(1):7-23.Jiang Shan,Dong Kejun,He Ming.Development and application of ultrasensitive accelerator mass spectrometry[J].Rock and Mineral Analysis,2012,31(1):7-23(in Chinese).
[13]王晓波,王伟,胡金君,等.加速器质谱在核科学研究中的应用进展[J].原子核物理评论,2013,30(2):161-165.Wang Xiaobo,Wang Wei,Hu Jinjun,et al.Application of accelerator mass spectrometry in nuclear science[J].Nuclear Physics Review,2013,30(2):161-165(in Chinese).
[14]赵庆章,杨宪林,林德雨,等.基于加速器质谱测量的41 Ca植物示踪样品制备方法研究[J].同位素,2016,29(3):193-197.Zhao Qingzhang,Jiang Pingping,Lin Deyu,et al.Preparation of plant 41 Ca tracer samples for accelerator mass spectrometry[J].Journal of Isotopes,2016,29(4):193-197(in Chinese).
[15]赵墨田,曹永明,陈刚,等.无机质谱概论[M].北京:化学工业出版社,2006:153-164.
[16]杨旭冉,庞义俊,何明,等.用于AMS测量的14 C样品制备方法[J].同位素,2015,28(2):65-68.Yang Xuran,Pang Yijun,He Ming,et al.Method of 14 C sample preparation for AMS measurement[J].Journal of Isotopes,2015,28(2):65-68(in Chinese).
[17]Choe K,Song S,Lee J H,et al.A Study on trapping CO2 by using a molecular sieve for 14 C AMS sample preparation[J].Radiocarbon,2013,55(2-3):421-425.
[18]Xu X,Trumbore S E,Zheng S,et al.Modifying a sealed tube zinc reduction method for preparation of AMS graphite targets:reducing background and attaining high precision[J].Nucl Instrum Meth B,2007,259(1):320-329.
[19]奚娴婷,丁杏芳,付东坡,等.用一年生植物研究大气Δ14 C分布与化石源CO2排放[J].科学通报,2011,56(13):1 026-1 031.
[20]庞义俊.14 C-AMS测量及其在PM2.5源解析中的应用[D].广西师范大学:国家图书馆,2016.
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