Highly sensitive lable-free electrochemical aptasensor for thrombin detection with cobalt hexacyanoferrate as the electrochemical probe

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• 作者：Shaoming Yang ; Hong Li ; Wenling Zha ; Qing Sun…
• 关键词：Cobalt hexacyanoferrates ; Thrombin ; Aptamer ; Electrochemical aptasensor ; Redox probe
• 刊名：Journal of Solid State Electrochemistry
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：17
• 期：10
• 页码：2603-2610
• 全文大小：600KB
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• 作者单位：Shaoming Yang (1)
  Hong Li (1)
  Wenling Zha (1)
  Qing Sun (1)
  Longzhen Zheng (1)
  Aixi Chen (1)
  
  1. Department of Chemistry and Chemical Engineering, East China Jiaotong University, 330013, Nanchang, People’s Republic China
  
• ISSN：1433-0768

文摘

A highly sensitive label-free electrochemical aptasensor has been constructed for the electrochemical detection of thrombin (TB), where two layers of cobalt hexacyanoferrate (CoHCF) redox probes sandwiched with carbon nanotubes–Nafion were directly immobilized on the electrode surface by electrodeposition. Through the strong interaction between CN?/sup> (CoHCF) and gold nanoparticles (GNPs), GNPs were assembled on the CoHCF-modified electrode for the immobilization of thiolated thrombin aptamers (TBA). In the presence of target TB, TBA on the electrode surface could catch TB to form TBA–TB complex, which made a barrier for the electron transfer, resulting in a greater decrease in CoHCF redox probe signals. Thus, the proposed aptasensor showed a high sensitivity and a much wider linearity to TB in the range of 1.0?pg/mL?～-.0?μg/mL with a detection limit of 0.28?pg/mL.