Prognostic significance of SERPINE2 in gastric cancer and its biological function in SGC7901 cells

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• 作者：Kun Wang (1)
  Bin Wang (1)
  Ai Yan Xing (2) (3)
  Ke Sen Xu (4)
  Guang Xin Li (1)
  Zhen Hai Yu (1)
  
  1. Department of General Surgery ; Qianfoshan Hospital ; Shandong University ; 16766 Jing Shi Road ; Jinan ; 250014 ; China
  2. Department of Pathology ; School of Medicine ; Shandong University ; 44 Wen Hua Xi Road ; Jinan ; 250012 ; China
  3. Department of Pathology ; Qilu Hospital ; Shandong University ; 107 Wen Hua Xi Road ; Jinan ; 250012 ; China
  4. Department of General Surgery ; Qilu Hospital ; Shandong University ; 107 Wen Hua Xi Road ; Jinan ; 250012 ; China
  
• 关键词：SERPINE2 ; Gastric cancer ; Prognosis ; Migration ; Invasion ; Growth ; Tumor aggressiveness
• 刊名：Journal of Cancer Research and Clinical Oncology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：141
• 期：5
• 页码：805-812
• 全文大小：993 KB
• 参考文献：1. Bergeron, S, Lemieux, E, Durand, V, Cagnol, S, Carrier, JC, Lussier, JG, Boucher, MJ, Rivard, N (2010) The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesis. Mol Cancer 9: pp. 271 CrossRef
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Cancer Research
  Internal Medicine
  Hematology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1335

文摘

Purpose Altered expression of serine protease inhibitor peptidase inhibitor clade E member 2 (SERPINE2) associates with human cancer development and progression; thus, this study investigated SERPINE2 expression in gastric cancer tissues for association with clinicopathological and survival data from the patients and then investigated the role of SERPINE2 in gastric cancer cells in vitro. Methods The levels of SERPINE2 mRNA in 243 gastric cancer tissues and paired non-cancerous mucosa were determined using quantitative PCR. Inhibition of SERPINE2 expression by small interfering RNA (siRNA) was detected by Western blotting. tetrazolium, soft agar, and transwell assays were performed to evaluate the proliferation, anchorage-independent growth, and motility of gastric cancer SGC7901 cells transfected with SERPINE2 siRNA. Results Compared with the normal mucosa, SERPINE2 mRNA was increased in gastric cancer tissues and cells. Analysis of the 243 matched specimens showed that high SERPINE2 levels were associated with lymph node metastasis, distant metastasis, and clinical stage. Patients with high SERPINE2 mRNA levels had poorer survival compared with patients with low SERPINE2 mRNA levels. In vitro, SERPINE2 inhibited anchorage-independent growth, migration, and invasion of SGC7901 cells, but not proliferation. Conclusions Our findings indicate that upregulated SERPINE2 may contribute to the aggressive phenotype of gastric cancer and suggest that SERPINE2 can be used as a novel prognostic factor and anticancer target in patients with gastric cancer.