Tegillarca granosa Extract Haishengsu Induces Apoptosis in Human Hepatocellular Carcinoma Cell Line BEL-7402 Via Fas-Signaling Pathways

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• 作者：Xuehong Chen (1)
  Yantao Han (1)
  Songmei Zhan (2)
  Chunbo Wang (1)
  Shouguo Chen (3)
  
  1. Medical College of Qingdao University ; Qingdao ; 266071 ; China
  2. The Affiliated Hospital of Qingdao University ; Qingdao ; 266071 ; China
  3. Qingdao Haihui Biochemical Pharmaceutical Company ; Qingdao ; 266031 ; China
  
• 关键词：Haishengsu ; BEL ; 7402 cells ; Apoptosis ; Hepatocellular carcinoma
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：71
• 期：2
• 页码：837-844
• 全文大小：903 KB
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• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

This study was designed to investigate the apoptosis-inducing properties of Tegillarca granosa extract Haishengsu (HSS) in human hepatocellular carcinoma cell line BEL-7402. Proliferation inhibition of the human hepatocellular carcinoma BEL-7402 cells was determined by the MTT assay, and the cell viability was determined by trypan blue dye exclusion assay. Apoptosis of BEL-7402 cells was demonstrated by fluorescence microscope with flow cytometry with Annexin V-FITC/PI double staining and Hoechst 33258 staining. Western blot analysis and RT-PCR were used to determine the expression levels of Fas. Expressions of caspase-8 and caspase-3 were examined by caspase activity assay and western blot analysis. HSS inhibited the proliferation of human hepatocellular carcinoma BEL-7402 cells in a dose- and time-dependent manner. Our results showed HSS had positive effect on apoptosis through flow cytometry assay and fluorescence microscope. The expressions of Fas protein and mRNA were up-regulated following the treatment. Caspase-8 and caspase-3 were activated in the cells cultured with HSS. In conclusion, HSS induced apoptosis of human hepatocellular carcinoma BEL-7402 cells. The apoptosis was associated with the up-regulation of Fas and the activations of caspase-8 and caspase-3.