Designing an Antibody-Based Chaperoning System through Programming the Binding and Release of the Folding Intermediate

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• 作者：Tingting Liu ; Caixian Sun ; Cong Li ; Jinhyuk Lee ; Yong-Doo Park ; Yixin Zhang ; Sen Li
• 刊名：ACS Chemical Biology
• 出版年：2016
• 出版时间：April 15, 2016
• 年：2016
• 卷：11
• 期：4
• 页码：1090-1097
• 全文大小：465K
• 年卷期：0
• ISSN：1554-8937

文摘

The protein folding pathway consists of sequential intramolecular interactions, while chaperones exert their functions either by stabilizing folding intermediates or by preventing nonspecific intermolecular interactions, which are often associated with aggregation involving exposed hydrophobic residues in folding intermediates. As chaperones do not possess specificity for individual client proteins, we designed an antibody-based chaperoning system to mimic the sequential binding and release of client proteins undergoing folding. The single-chain variable fragment of antibody (scFv) A4 binds to human muscle creatine kinase (HCK) and prevents it from aggregating. The slow dissociation of HCK from A4 resulted in delayed but eventually high-quality refolding, as reflected by the higher recovery of enzymatic activity as well as abolished aggregation. Peptide P6, a sequence in HCK involved in A4 binding, competes with HCK, promotes its dissociation from A4, and accelerates the rate of high-quality refolding. The sequential addition of A4 and P6 is essential for the chaperoning effect. The programmed binding/release method can also be applied to refold HCK from inclusion bodies. Because the association/dissociation of the folding intermediate with the antibody is highly specific, the method can be used to design tailored refolding systems and to investigate chaperoning effects on protein folding/aggregation in a sequence-specific manner.