Synthesized Peptides from Yam Dioscorin Hydrolysis in Silico Exhibit Dipeptidyl Peptidase-IV Inhibitory Activities and Oral Glucose Tolerance Improvements in Normal Mice

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• 作者：Yin-Shiou Lin ; Chuan-Hsiao Han ; Shyr-Yi Lin ; Wen-Chi Hou
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：August 24, 2016
• 年：2016
• 卷：64
• 期：33
• 页码：6451-6458
• 全文大小：501K
• 年卷期：0
• ISSN：1520-5118

文摘

RRDY, RL, and DPF were the top 3 of 21 peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico and were further investigated in a proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by a feeding gauge; 30 min later, the glucose loads (2.5 g/kg) were performed. RRDY, yam dioscorin, or sitagliptin preload, but not DPF, lowered the area under the curve (AUC_0–120) of blood glucose and DPP-IV activity and elevated the AUC_0–120 of blood insulin, which showed significant differences compared to control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic control in normal mice and need further investigations in diabetic animal models.