MC3T3-E1 Cell Response to Ti1‿i>xAgx and Ag-TiNx Electrodes Deposited on Piezoelectric Poly(vinylidene fluoride) Substrates for Sensor Applications

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• 作者：S. M. Marques ; P. Rico ; I. Carvalho ; J. L. Gómez Ribelles ; L. Fialho ; S. Lanceros-Méndez ; M. Henriques ; S. Carvalho
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：February 17, 2016
• 年：2016
• 卷：8
• 期：6
• 页码：4199-4207
• 全文大小：538K
• ISSN：1944-8252

文摘

In the sensors field, titanium based coatings are being used for the acquisition/application of electrical signals from/to piezoelectric materials. In this particular case, sensors are used to detect dynamic mechanical loads at early stages after intervention of problems associated with prostheses implantation. The aim of this work is to select an adequate electrode for sensor applications capable, in an initial stage to avoid bone cell adhesion, but at a long stage, permit osteointegration and osteoinduction. This work reports on the evaluation of osteoblast MC3T3-E1 cells behavior in terms of proliferation, adhesion and long-term differentiation of two different systems used as sensor electrodes: Ti_1–xAg_x and Ag-TiN_x deposited by d.c. and pulsed magnetron sputtering at room temperature on poly(vinylidene fluoride) (PVDF). The results indicated an improved effect of Ag-TiN_x electrodes compared with Ti_1–xAg_x and TiN, in terms of diminished cell adhesion and proliferation at an initial cell culture stage. Nevertheless, when cell culture time is longer, cells grown onto Ag-TiN_x electrodes are capable to proliferate and also differentiate at proper rates, indicating the suitability of this coating for sensor application in prostheses devices. Thus, the Ag-TiN_x system was considered the most promising electrode for tissue engineering applications in the design of sensors for prostheses to detect dynamic mechanical loads.