Enhancement of Enzymatic Activity Using Microfabricated Poly(ε-caprolactone)/Silica Hybrid Microspheres with Hierarchically Porous Architecture

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• 作者：Yimei Fan ; Xiaodong Cao ; Tao Hu ; Xiaoguang Lin ; Hua Dong ; Xuenong Zou
• 刊名：Journal of Physical Chemistry C
• 出版年：2016
• 出版时间：February 25, 2016
• 年：2016
• 卷：120
• 期：7
• 页码：3955-3963
• 全文大小：633K
• ISSN：1932-7455

文摘

In this paper, we present a novel and facile microfluidic method to fabricate hierarchically porous poly(ε-caprolactone)/silica hybrid microspheres and further investigate in detail their performance as enzyme carriers by three famous proteins and enzymes. Because of the synergy effect between sol–gel process and solvent extraction in microdroplets, hierarchically porous architecture can be formed in situ without the use of porogens and templates. More importantly, the surface porosity or the specific surface area of such microspheres can be precisely tuned via adjusting the hydrolysis/condensation rate by ammonia catalyst and thus the competition between the two above-mentioned processes. Fluorescein isothiocyanate-bovine serum albumin, alcohol dehydrogenase, and superoxide dismutase are immobilized via either physical adsorption or covalent binding to evaluate the performance of hierarchically porous microspheres as enzyme carriers. All the qualitative and quantitative data including fluorescence images, enzymatic activity, immobilization yield, and activity yield prove that enzymes covalently immobilized on hierarchically porous microspheres exhibit the optimal immobilization capacity, enzymatic activity, stability, and reusability, which shows very promising application of such microspheres in enzymatic catalysis.