Proteomic Profile of Unstable Atheroma Plaque: Increased Neutrophil Defensin 1, Clusterin, and Apolipoprotein E Levels in Carotid Secretome

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• 作者：Gemma Aragonès ; Teresa Auguet ; Esther Guiu-Jurado ; Alba Berlanga ; Marta Curriu ; Salomé Martinez ; Ajla Alibalic ; Carmen Aguilar ; Esteban Hernández ; María-Luisa Camara ; Núria Canela ; Pol Herrero ; Xavier Ruyra ; Vicente Martín-Paredero ; Cristóbal Richart
• 刊名：Journal of Proteome Research
• 出版年：2016
• 出版时间：March 4, 2016
• 年：2016
• 卷：15
• 期：3
• 页码：933-944
• 全文大小：439K
• ISSN：1535-3907

文摘

Because of the clinical significance of carotid atherosclerosis, the search for novel biomarkers has become a priority. The aim of the present study was to compare the protein secretion profile of the carotid atherosclerotic plaque (CAP, n = 12) and nonatherosclerotic mammary artery (MA, n = 10) secretomes. We used a nontargeted proteomic approach that incorporated tandem immunoaffinity depletion, iTRAQ labeling, and nanoflow liquid chromatography coupled to high-resolution mass spectrometry. In total, 162 proteins were quantified, of which 25 showed statistically significant differences in secretome levels between carotid atherosclerotic plaque and nondiseased mammary artery. We found increased levels of neutrophil defensin 1, apolipoprotein E, clusterin, and zinc-alpha-2-glycoprotein in CAP secretomes. Results were validated by ELISA assays. Also, differentially secreted proteins are involved in pathways such as focal adhesion and leukocyte transendothelial migration. In conclusion, this study provides a subset of identified proteins that are differently expressed in secretomes of clinical significance.