Integrated Microcapillary for Sample-to-Answer Nucleic Acid Pretreatment, Amplification, and Detection

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• 作者：Lu Zhang ; Yi Zhang ; Chunyan Wang ; Qiang Feng ; Fei Fan ; Guojun Zhang ; Xixiong Kang ; Xuzhen Qin ; Jiashu Sun ; Yinghui Li ; Xingyu Jiang
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：October 21, 2014
• 年：2014
• 卷：86
• 期：20
• 页码：10461-10466
• 全文大小：351K
• ISSN：1520-6882

文摘

This work develops an integrated microcapillary-based loop-mediated isothermal amplification (icLAMP) containing preloaded reagents and DNA extraction card, allowing for sample-to-answer screening of single nucleotide polymorphisms (SNPs) typing of the CYP2C19 gene from untreated blood samples with minimal user operation. With all reagents and the DNA extraction card preloaded inside the capillary, this icLAMP system can achieve on-site pretreatment, extraction, amplification, and detection of nucleic acids within 150 min, without the requirement for advanced instruments. As icLAMP technology carries many advantages such as disposability, easy operation, low cost, and reduced cross contamination and biohazard risks, we expect this system to have a great impact on point-of-care (POC) nucleic acid detection.