Quantitative Analysis of the Human AKR Family Members in Cancer Cell Lines Using the mTRAQ/MRM Approach

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• 作者：Shenyan Zhang ; Bo Wen ; Baojin Zhou ; Lei Yang ; Chao Cha ; Shaoxing Xu ; Xuemei Qiu ; Quanhui Wang ; Haidan Sun ; Xiaomin Lou ; Jin Zi ; Yong Zhang ; Liang Lin ; Siqi Liu
• 刊名：Journal of Proteome Research
• 出版年：2013
• 出版时间：May 3, 2013
• 年：2013
• 卷：12
• 期：5
• 页码：2022-2033
• 全文大小：433K
• 年卷期：v.12,no.5(May 3, 2013)
• ISSN：1535-3907

文摘

Members of human aldo-keto reductase (AKR) superfamily have been reported to be involved in cancer progression, whereas the final conclusion is not generally accepted. Herein, we propose a quantitative method to measure human AKR proteins in cells using mTRAQ-based multiple reaction monitoring (MRM). AKR peptides with multiple transitions were carefully selected upon tryptic digestion of the recombinant AKR proteins, while AKR proteins were identified by SDS-PAGE fractionation coupled with LC鈥揗S/MS. Utilizing mTRAQ triplex labeling to produce the derivative peptides, calibration curves were generated using the mixed lysate as background, and no significantly different quantification of AKRs was elicited from the two sets of calibration curves under the mixed and single lysate as background. We employed this approach to quantitatively determine the 6 AKR proteins, AKR1A1, AKR1B1, AKR1B10, AKR1C1/C2, AKR1C3, and AKR1C4, in 7 different cancer cell lines and for the first time to obtain the absolute quantities of all the AKR proteins in each cell. The cluster plot revealed that AKR1A and AKR1B were widely distributed in most cancer cells with relatively stable abundances, whereas AKR1Cs were unevenly detected among these cells with diverse dynamic abundances. The AKR quantitative distribution in different cancer cells, therefore, may assist further exploration toward how the AKR proteins are involved in tumorigenesis.

Keywords:

MRM; AKR; mTRAQ labeling; absolute quantification; cancer cell lines