Supramolecular Immobilization of Xanthine Oxidase on Electropolymerized Matrix of Functionalized Hybrid Gold Nanoparticles/Single-Walled Carbon Nanotubes for the Preparation of Electrochemical Biosens

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• 作者：Reynaldo Villalonga ; Paula D铆ez ; Marcos Egu铆laz ; Paloma Mart铆nez ; Jos茅 M. Pingarr贸n
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2012
• 出版时间：August 22, 2012
• 年：2012
• 卷：4
• 期：8
• 页码：4312-4319
• 全文大小：406K
• 年卷期：v.4,no.8(August 22, 2012)
• ISSN：1944-8252

文摘

Glassy carbon electrodes modified with single-walled carbon nanotubes and a three-dimensional network of electropolymerized Au nanoparticles capped with 2-mercaptoethanesulfonic acid, p-aminothiophenol, and 1-adamantanethiol were used as hybrid electrochemical platforms for supramolecular immobilization of a synthesized artificial neoglycoenzyme of xanthine oxidase and 尾-cyclodextrin through host鈥揼uest interactions. The ensemble was further employed for the bioelectrochemical determination of xanthine. The biosensor showed fast amperometric response within 5 s and a linear behavior in the 50 nM to 9.5 渭M xanthine concentration range with high sensitivity, 2.47 A/(M cm²), and very low detection limit of 40 nM. The stability of the biosensor was significantly improved and the interferences caused by ascorbic and uric acids were noticeably minimized by coating the electrode surface with a Nafion thin film.

Keywords:

single-walled carbon nanotube; gold nanoparticle; supramolecular assembly; enzyme biosensor; cyclodextrin