Probing Solvent Fractionation in Electrospray Droplets with Laser-Induced Fluorescence of a Solvatochromic Dye

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• 作者：Shaolian Zhou and Kelsey D. Cook
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：March 1, 2000
• 年：2000
• 卷：72
• 期：5
• 页码：963 - 969
• 全文大小：92K
• 年卷期：v.72,no.5(March 1, 2000)
• ISSN：1520-6882

文摘

Laser-induced fluorescence spectroscopy is used to profile solvent fractionation in an electrospray plume containing Nile Red, a solvatochromic dye (i.e., a dye for whichspectral features are sensitive to solvent polarity). Theresults confirm gradual enrichment of the less volatilecomponent in spray droplets as a result of solventevaporation. Changes in solvent composition are evidentin both axial and lateral profiles. The influences ofcapillary voltage, nebulizing gas, an electrolyte modifier,and flow rate on the extent of solvent fractionation areassessed. The results suggest that, in addition to volatilityof the solvents, major factors affecting solvent fractionationinclude initial droplet size, droplet velocity, and thecooling effect of the nebulizing gas. Isolating contributionsfrom these factors will require complementary data fromother experiments.