Liposomes Physically Coated with Peptides: Preparation and Characterization

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• 作者：Cuicui Su ; Yuqiong Xia ; Jianbo Sun ; Nan Wang ; Lin Zhu ; Tao Chen ; Yanyi Huang ; Dehai Liang
• 刊名：Langmuir
• 出版年：2014
• 出版时间：June 3, 2014
• 年：2014
• 卷：30
• 期：21
• 页码：6219-6227
• 全文大小：380K
• 年卷期：v.30,no.21(June 3, 2014)
• ISSN：1520-5827

文摘

Physically coating liposomes with peptides of desirable functions is an economic, versatile, and less time-consuming approach to prepare drug delivery vehicles. In this work, we designed three peptides鈥擜c-WWKKKGGNNN-NH2 (W2K3), Ac-WWRRRGGNNN-NH2(W2R3), Ac-WWGGGGGNNN-NH2(W2G3)鈥攁nd studied their coating ability on negatively charged liposomes. It was found that the coating was mainly driven by the electrostatic interaction between the peptides鈥?cationic side groups and the acidic lipids, which also mediated the 鈥渁nchoring 鈥?of Trp residuals in the interfacial region of lipid bilayers. At the same conditions, the amount of the coated W2R3 was more than that of W2K3, but the stability of the liposome coated with W2R3 was deteriorated. This was caused by the delocalized charge of the guanidinium group of arginine. The coating of the peptide rendered the liposome pH-responsive behavior but did not prominently change the phase transition temperature. The liposome coated with peptides displayed appropriate pH/temperature dual responsive characteristics and was able to release the content in a controlled manner.