Cell Screening Using Disposable Photonic Lab on a Chip Systems

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• 作者：Bergoi Ibarlucea ; Elisabet Fernandez-Rosas ; Jordi Vila-Planas ; Stefanie Demming ; Carme Nogues ; Jose A. Plaza ; Stephanus Bttgenbach ; Andreu Llobera
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：May 15, 2010
• 年：2010
• 卷：82
• 期：10
• 页码：4246-4251
• 全文大小：304K
• 年卷期：v.82,no.10(May 15, 2010)
• ISSN：1520-6882

文摘

A low-cost photonic lab on a chip with three different working regimes for cell screening is presented. The proposed system is able to perform scattering, scattering + absorption, and absorption measurements without any modification. Opposite to the standard flow cytometers, in this proposed configuration, a single 30 ms scan allows to obtain information regarding the cell optical properties. An additional novelty is that the whole spectrum is obtained and analyzed, being then possible to determine for each regime which is the optimal working wavelength that would provide the best performance in terms of sensitivity and limit of detection (LOD). Experimental results have provided with an LOD of 54.9 ± 0.7 cells (in the scattering regime using unlabeled cells), 53 ± 1 cells (in the scattering + absorption regime using labeled cells), and 105 ± 4 cells (in the absorption regime using labeled cells). Finally, the system has also been used for measuring the dead/live cell ratio, obtaining LODs between 7.6 ± 0.4% and 6.7 ± 0.3%, depending on the working regime used.