Locations and Reorientations of Multi-Ring-Fused 2-Pyridones in Ganglioside GM1 Micelles

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• 作者：Radek S虒achl ; Erik Rosenbaum ; Magnus Sellstedt ; Fredrik Almqvist ; Lennart B.-脜. Johansson
• 刊名：Langmuir
• 出版年：2011
• 出版时间：March 1, 2011
• 年：2011
• 卷：27
• 期：5
• 页码：1662-1667
• 全文大小：800K
• 年卷期：v.27,no.5(March 1, 2011)
• ISSN：1520-5827

文摘

Fluorescent multi-ring-fused 2-pyridones, with chemical resemblance to other biologically active 2-pyridone systems, were solubilized in spherical micelles formed by the gangloiside G_M1 and studied with respect to their spatial localization and rotational mobility. For this, electronic energy transfer between the multi-ring-fused 2-pyridone (donor) and BODIPY-FL-labeled G_M1 was determined, as well as their fluorescence depolarization. From the obtained efficiency of energy transfer to the acceptor group (BODIPY-FL), either localized in the polar or in the nonpolar part of the ganglioside, it has been possible to estimate the most likely localization of the multi-ring-fused 2-pyridones. The center of mass of the studied multi-ring-fused 2-pyridones are located at approximately 33 脜 from the micellar center of mass, which corresponds to the internal hydrophobic鈭抙ydrophilic interfacial region. At this location, the reorienting rates of the multi-ring-fused 2-pyridones are surprisingly slow with typical correlation times of 35鈭?5 ns. No evidence was found for the formation of ground and excited state dimers, even when two monomers were forced to be near each other via a short covalent linker.